Seed Germination and Reserve Mobilization

ABSTRACT: Erythrina velutina Willd. (Fabaceae) is a tree species that bears social, ecological, and economic importance for northeastern Brazil, a region with frequent temperature variations. The objective of this work was to evaluate the mobilization of reserves during seed germination and seedling establishment of E. velutina at different temperatures, and how these mechanisms are affected in a thermal-stress situation. The seeds germinated at 5, 15, 25, 35, and 45 °C, and the physiological and biochemical aspects of reserve mobilization (starch, lipids, and total proteins) were assessed during seedling establishment. Germination, vigor, and the mobilization of reserves from the cotyledons to the embryonic axis are affected according to the incubation temperature. The temperature of 25 °C favors the mobilization of reserves during seed germination and seedling establishment in E. velutina. In a situation of thermal stress, high temperatures affect the establishment of the seedlings, whereas low temperatures compromise the mobilization of reserves.

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Humic acid improved germination rate, seedling growth and antioxidant system of pea (Pisum sativum L. var. Alicia) grown in water polluted with CdCl2

AIMS Environmental Science ◽

10.3934/environsci.2021023 ◽

2021 ◽

Vol 8 (4) ◽

pp. 358-370

Author(s):

Mohammed Basahi ◽

Keyword(s):

Oxidative Stress ◽

Humic Acid ◽

Seed Germination ◽

Pisum Sativum ◽

Seedling Growth ◽

Antioxidant System ◽

Germination Rate ◽

Guaiacol Peroxidase ◽

Reserve Mobilization ◽

Plant Life

<abstract> <p>Seeds of pea (<italic>Pisum sativum</italic> L. var. Alicia) were germinated for 5 d by soaking in distilled water or treated with cadmium and/or humic acid (HA). Interaction between humic acid and cadmium was previously discussed. However, the regulation of the humic acid / cadmium interaction during seed germination, the first crucial stage of the plant life cycle, need to be clarified. Seed germination is an important stage of the plant life, which is highly sensitive to surrounding medium changes, since the germinating seed is the first interface of material exchange between plant cycle and environment. Relationships among cadmium stress, HA-treatment, germination rate, and changes in reserve mobilization were studied. Cadmium disrupted the soaking process that was a major event of germination. Moreover, important solutes leakage due to Cd-treatment caused disorder in reserve mobilization from cotyledons to growing embryonic axes. The observed delay in germination rate and seedling growth after Cd-treatment could be explained, partially, by the disturbance in the mobilization of water and nutrients. NADH-and MDA-activities were considered as markers of oxidative stress. they were substantially increased after Cd-treatment compared with controls but decreased in presence of AH. Excessive NADH oxidase activities proved the install of oxidative stress in Cd poisoned seeds. We suggested an antagonist effect of Cd and HA on oxidative stress enzyme activities. Treatment of seeds with Cd and/or AH maintained a high level of guaiacol peroxidase activities during five days. The possible implication of GPOX against Cd toxicity and the improvement of the antioxidant system after AH treatment proved an important implication of these findings during germination.</p> </abstract>

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Seed germination of Egyptian Pancratium maritimum under salinity with regard to cytology, antioxidant and reserve mobilization enzymes, and seed anatomy

Flora ◽

10.1016/j.flora.2018.03.011 ◽

2018 ◽

Vol 242 ◽

pp. 120-127 ◽

Cited By ~ 2

Author(s):

Elsayed Mohamed ◽

Ahmed M.M. Kasem ◽

Kotb A. Farghali

Keyword(s):

Seed Germination ◽

Reserve Mobilization ◽

Seed Anatomy ◽

Pancratium Maritimum

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Seed soaking with melatonin promotes seed germination under chromium stress via enhancing reserve mobilization and antioxidant metabolism in wheat

Ecotoxicology and Environmental Safety ◽

10.1016/j.ecoenv.2021.112241 ◽

2021 ◽

Vol 220 ◽

pp. 112241

Author(s):

Kangqi Lei ◽

Shuzhen Sun ◽

Kaitai Zhong ◽

Shiyu Li ◽

Hang Hu ◽

...

Keyword(s):

Seed Germination ◽

Antioxidant Metabolism ◽

Reserve Mobilization ◽

Seed Soaking ◽

Chromium Stress

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A genome-wide association study reveals that the cytochrome b5 involved in seed reserve mobilization during seed germination in rice

Theoretical and Applied Genetics ◽

10.1007/s00122-021-03948-2 ◽

2021 ◽

Author(s):

Zhibo Huang ◽

Jifeng Ying ◽

Liling Peng ◽

Shan Sun ◽

Chengwei Huang ◽

...

Keyword(s):

Seed Germination ◽

Association Study ◽

Genome Wide Association Study ◽

Cytochrome B5 ◽

Genome Wide Association ◽

Reserve Mobilization ◽

Genome Wide ◽

A Genome

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Seed Priming With an Animal-derived Protein Hydrolysate Improves Drought Tolerance of Tomato Seeds by Enhancing Reserve Mobilization, Osmotic Adjustment, and Antioxidant Mechanism

10.21203/rs.3.rs-939786/v1 ◽

2021 ◽

Author(s):

Weixuan Wang ◽

Chenglong Zhang ◽

Wenlong Zheng ◽

Haofeng Lv ◽

Weiwei Zhou ◽

...

Keyword(s):

Drought Stress ◽

Seed Germination ◽

Seedling Growth ◽

Soluble Sugar ◽

Seed Priming ◽

Protein Hydrolysates ◽

Tomato Seed ◽

Reserve Mobilization ◽

Tomato Seeds ◽

Germination Traits

Abstract Purpose Protein hydrolysates obtained from agro-industrial byproducts have received much attentions due to their positive roles in regulating plant responses to environmental stresses. However, little is known about the roles of animal protein hydrolysates in mediating seed drought tolerance and the underlying mechanism. Here, the effects of seed priming with pig blood protein hydrolysates (PP) on tomato seed germination and seedling growth under drought stress were investigated. Methods Tomato seeds were soaked with different concentrations of PP solutions for 24 h, and then transferred to filter paper moistened with distilled water or 10% PEG-6000 solution in Petri dish. The germination traits, seeding growth, reserve mobilization, osmolytes, and antioxidant system were determined.Results PP priming effectively alleviated the reduction in seed germination traits, resulting in improved tomato seedling growth under drought stress. PP priming enhanced amylase and sucrose synthase activities, soluble sugar, soluble protein, and free amino acid levels, thereby promoting reserve mobilization in seeds. Moreover, PP priming also reduces osmotic toxicity by increasing the accumulation of proline, soluble protein, and soluble sugar. Drought stress substantially enhanced the production of ROS and subsequent increases in MDA and Evans blue uptake, which were significantly alleviated after PP priming by improving the activities of SOD, POD, and CAT, and non-enzymatic antioxidants. Conclusion PP priming is a feasible method for improving tomato seed germination and seedling growth under drought stress by enhancing reserve mobilization, osmolyte accumulation, and antioxidant systems.

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