Rapid Fibroblast Cell Culture Characterization with Impedimetric Label‐free Heat Shock Protein – 47 Biosensor

2020 ◽  
Vol 32 (10) ◽  
pp. 2310-2315
Author(s):  
Duygu Harmanci ◽  
Zihni Onur Uygun ◽  
Ayşe Koçak Sezgin ◽  
Cenk Demirdöver ◽  
Ferhan Girgin Sagin ◽  
...  
Keyword(s):  
Cell Culture ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Fibroblast Cell ◽  
Label Free ◽  
Heat Shock Protein 47 ◽  
Fibroblast Cell Culture

Treatment of Idiopathic Myelofibrosis Employing siRNA for Heat Shock Protein 47 (siRNA/HSP47) Encapsulated in Liposomes.

Blood ◽  
2007 ◽  
Vol 110 (11) ◽  
pp. 4646-4646
Author(s):  
Junichi Watanabe ◽  
Takuya Matsunaga ◽  
Kazuyuki Murase ◽  
Yasushi Sato ◽  
Taiko Nagaya ◽  
...  
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Fibroblast Cell ◽  
Idiopathic Myelofibrosis ◽  
Hematopoietic Stem ◽  
Heat Shock Protein 47 ◽  
Collagen Secretion ◽  
Novel Approach ◽  
Murine Fibroblast ◽  
Chronic Myeloproliferative Disorder

Abstract Idiopathic myelofibrosis (IMF) is a chronic myeloproliferative disorder characterized by bone marrow (BM) fibrosis, splenomegaly, extramedurally hematopoiesis and anemia. At present, there is no curative treatment available for this disease except allogeneic hematopoietic stem cell transplantation (allo-HSCT). However, the high therapy-related mortality of allo-HSCT, even in reduced-intensity transplantation, could not be ignored. We report a novel approach for the treatment of IMF, utilizing siRNA for a collagen specific chaperon, heat shock protein 47 (HSP47) encapsulated in liposomes (lip-siRNA/HSP47). We first confirmed suppression of HSP47 in NIH3T3 murine fibroblast cell line and primary murine BM fibroblasts, and collagen secretion from these cells by siRNA/HSP47. We next treated thrombopoietin (TPO) transgenic mice exhibiting IMF like feature (Shimoda K, Harada M et al. Leuk Res29, 761–769, 2005; Am J Hematol82, 802–806, 2007). In these mice, treatment by intravenous injection of lip-siRNA/HSP47 resulted in histological resolution of myelofibrosis associated with apoptotic death of BM fibroblasts, and improvement of anemia and splenomegaly. Thus, lip-siRNA/HSP47 therapy may be a promising treatment for IMF.

Increased expression of collagen-binding heat shock protein 47 in murine bleomycin-induced pneumopathy

2003 ◽  
Vol 285 (4) ◽  
pp. L957-L963 ◽  
Author(s):  
Hiroshi Ishii ◽  
Hiroshi Mukae ◽  
Tomoyuki Kakugawa ◽  
Tetsuji Iwashita ◽  
Hideyuki Kaida ◽  
...  
Keyword(s):  
Heat Shock ◽  
Pulmonary Fibrosis ◽  
Heat Shock Protein ◽  
Smooth Muscle Actin ◽  
Protein A ◽  
Immunohistochemical Analysis ◽  
Rt Pcr ◽  
Heat Shock Protein 47 ◽  
Positive Type ◽  
Fibrotic Process

The 47-kDa heat shock protein 47 (HSP47) is a collagen-specific molecular chaperone that has been shown to play a major role during the processing and/or secretion of procollagen. Expression of HSP47 has been reported to increase in parallel with expression of collagens during the progression of various fibrosis models. The aim of the present study was to investigate the association between HSP47 expression and collagen accumulation in bleomycin (BLM)-induced murine fibrosis. We investigated the expression of HSP47 protein and mRNA using immunohistochemical analysis and semi-quantitative RT-PCR in murine BLM-induced pulmonary fibrosis. Immunohistochemical analysis showed that higher expression of HSP47 protein was present in BLM-induced pulmonary fibrosis compared with controls. HSP47 was localized predominantly in α-smooth muscle actin-positive myofibroblasts, F4/80 negative, surfactant protein-A-positive type II pneumocytes, and F4/80-positive macrophages. RT-PCR also demonstrated an increase of HSP47 mRNA expression in BLM-treated lungs. Moreover, the relative amounts of HSP47 mRNA correlated significantly with the lung hydroxyproline content as an indicator of pulmonary fibrosis in BLM-treated lungs ( r = 0.406, P <0.05). Our results suggest that these cells may play a role in the fibrotic process of BLM-treated lungs through upregulation of HSP47.

Heat Shock Protein 47 (HSP47)

2018 ◽  
pp. 1-7
Author(s):  
Fatin Hazwani Ibrahim ◽  
Normala Abd Latip ◽  
Mohd Firdaus Abdul-Wahab
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Heat Shock Protein 47

Human foreskin fibroblasts: from waste bag to important biomedical applications

2018 ◽  
Vol 11 (6) ◽  
pp. 385-394 ◽  
Author(s):  
Thomaz Oliveira ◽  
Ilana Costa ◽  
Victor Marinho ◽  
Valécia Carvalho ◽  
Karla Uchôa ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Culture ◽  
Experimental Studies ◽  
Embryonic Stem ◽  
Fibroblast Cell ◽  
Human Foreskin Fibroblast ◽  
Cellular Interactions ◽  
Human Foreskin Fibroblasts ◽  
Fibroblast Cell Culture ◽  
Human Foreskin Fibroblast Cell

Circumcision is one of the most performed surgical procedures worldwide, and it is estimated that one in three men worldwide is circumcised, which makes the preputial skin removed after surgery an abundant material for possible applications. In particular, it is possible efficiently to isolate the cells of the foreskin, with fibroblasts being the most abundant cells of the dermis and the most used in biomedical research. This work aimed to review the knowledge and obtain a broad view of the main applications of human foreskin fibroblast cell culture. A literature search was conducted, including clinical trials, preclinical basic research studies, reviews and experimental studies. Several medical and laboratory applications of human foreskin fibroblast cell culture have been described, especially when it comes to the use of human foreskin fibroblasts as feeder cells for the cultivation of human embryonic stem cells, in addition to co-culture with other cell types. The culture of foreskin fibroblasts has also been used to: obtain induced pluripotent stem cells; the diagnosis of Clostridium difficile; to test the toxicity and effect of substances on normal cells, especially the toxicity of possible antineoplastic drugs; in viral culture, mainly of the human cytomegalovirus, study of the pathogenesis of other microorganisms; varied studies of cellular physiology and cellular interactions. Fibroblasts are important for cell models for varied application cultures, demonstrating how the preputial material can be reused, making possible new applications. Level of evidence: Not applicable for this multicentre audit.

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