Laminaria japonica cyclic peptides exert anti‐colorectal carcinoma effects through apoptosis induction in vitro and in vivo

Four Pt(II) complexes of the general formula [Pt(L)(5,6-epoxy-1,10-phen)], where L is anion of malonic (mal, Pt1), 2-methylmalonic (Me-mal, Pt2), 2,2-dimethylmalonic (Me2-mal, Pt3) or 1,1- cyclobutanedicarboxylic (CBDCA, Pt4) acid while 5,6-epoxy-1,10-phen is bidentately coordinated 5,6-epoxy-5,6-dihydro-1,10-phenanthroline were synthesized and characterized by elemental microanalysis, IR, UV-Vis and NMR (1H and 13C) spectroscopic techniques. In vitro anticancer activity of novel platinum(II) complexes have been investigated on human and murine cancer cell lines, as well as normal murine cell line by MTT assay. The obtained results indicate that studied platinum(II) complexes exhibited strong cytotoxic activity against murine breast carcinoma cells (4T1), human (HCT116) and murine (CT26) colorectal carcinoma cells. Complex Pt3 display stronger selectivity toward carcinoma cells in comparison to other tested platinum(II) complexes exhibiting beneficial antitumor activity mainly via the induction of apoptosis, as well as inhibition of cell proliferation and migration. Further study showed that Pt3 complex also carry significant in vivo antitumor activity in orthotopical 4T1 tumor model without detected liver, kidney, lung, and heart toxicity. All results imply that these novel platinum(II) complexes have a good anti-tumor effect on breast and colorectal cancer in vivo and in vitro and the affinity to become possible candidates for treatment in anticancer therapy.

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Anti-Diabetic Effects and Anti-Inflammatory Effects of Laminaria japonica and Hizikia fusiforme in Skeletal Muscle: In Vitro and In Vivo Model

Nutrients ◽

10.3390/nu10040491 ◽

2018 ◽

Vol 10 (4) ◽

pp. 491 ◽

Cited By ~ 11

Author(s):

Sae-ym Kang ◽

Eunyoung Kim ◽

Inhae Kang ◽

Myoungsook Lee ◽

Yunkyoung Lee

Keyword(s):

Skeletal Muscle ◽

Laminaria Japonica ◽

In Vivo Model ◽

Hizikia Fusiforme ◽

Anti Inflammatory

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Apoptosis induction and AKT/NF-κB inactivation are associated with regroafenib-inhibited tumor progression in non-small cell lung cancer in vitro and in vivo

Biomedicine & Pharmacotherapy ◽

10.1016/j.biopha.2019.109032 ◽

2019 ◽

Vol 116 ◽

pp. 109032 ◽

Cited By ~ 8

Author(s):

Mao-Chi Weng ◽

Ming-Hsin Li ◽

Jing Gung Chung ◽

Yu-Chang Liu ◽

Jeng-Yuan Wu ◽

...

Keyword(s):

Lung Cancer ◽

Small Cell Lung Cancer ◽

Tumor Progression ◽

Cell Lung Cancer ◽

Small Cell ◽

Apoptosis Induction ◽

Small Cell Lung

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Protective Effect of Fucoxanthin Isolated from Laminaria japonica against Visible Light-Induced Retinal Damage Both in Vitro and in Vivo

Journal of Agricultural and Food Chemistry ◽

10.1021/acs.jafc.5b05436 ◽

2016 ◽

Vol 64 (2) ◽

pp. 416-424 ◽

Cited By ~ 16

Author(s):

Yixiang Liu ◽

Meng Liu ◽

Xichun Zhang ◽

Qingchou Chen ◽

Haixiu Chen ◽

...

Keyword(s):

Visible Light ◽

Protective Effect ◽

Laminaria Japonica ◽

Retinal Damage

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TREM2 Acts as a Tumor Suppressor in Colorectal Carcinoma through Wnt1/β-catenin and Erk Signaling

Cancers ◽

10.3390/cancers11091315 ◽

2019 ◽

Vol 11 (9) ◽

pp. 1315 ◽

Cited By ~ 3

Author(s):

Su-Man Kim ◽

Eun-Mi Kim ◽

Kon-Young Ji ◽

Hwa-Youn Lee ◽

Su-Min Yee ◽

...

Keyword(s):

Colorectal Cancer ◽

Tumor Suppressor ◽

Colorectal Carcinoma ◽

D1 Protein ◽

Erk Signaling ◽

In Vitro Assays ◽

Related Factors ◽

Human Colon Cancer

TREM2 (triggering receptor expressed on myeloid cells) is involved in the development of malignancies. However, the function of TREM2 in colorectal cancer has not been clearly elucidated. Here, we investigated TREM2 function for the first time in colorectal epithelial cancer cells and demonstrated that TREM2 is a novel tumor suppressor in colorectal carcinoma. Blockade of TREM2 significantly promoted the proliferation of HT29 colorectal carcinoma cells by regulating cell cycle-related factors, such as p53 phosphorylation and p21 and cyclin D1 protein levels. HT29 cell migration was also increased by TREM2 inhibition via MMP9 (matrix metalloproteinase 9) expression upregulation. Furthermore, we found that the tumor suppressor effects of TREM2 were associated with Wnt/β-catenin and extracellular signal-regulated kinase (ERK) signaling. Importantly, the effect of TREM2 in the suppression of tumor development was demonstrated by in vivo and in vitro assays, as well as in human colon cancer patient tissue arrays. Overall, our results identify TREM2 as a potential prognostic biomarker and therapeutic target for colorectal cancer.

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A Limulus Antilipopolysaccharide Factor-Derived Peptide Exhibits a New Immunological Activity with Potential Applicability in Infectious Diseases

Clinical and Diagnostic Laboratory Immunology ◽

10.1128/cdli.7.4.669-675.2000 ◽

2000 ◽

Vol 7 (4) ◽

pp. 669-675 ◽

Cited By ~ 28

Author(s):

Maribel G. Vallespi ◽

Luis A. Glaria ◽

Osvaldo Reyes ◽

Hilda E. Garay ◽

Joel Ferrero ◽

...

Keyword(s):

Infectious Diseases ◽

Cyclic Peptides ◽

Mononuclear Cells ◽

Therapeutic Potential ◽

Peritoneal Macrophages ◽

Induced Response ◽

No Production ◽

Human Mononuclear Cells

ABSTRACT Previous studies have shown that cyclic peptides corresponding to residues 35 to 52 of the Limulus antilipopolysaccharide (anti-LPS) factor (LALF) bind and neutralize LPS-mediated in vitro and in vivo activities. Therapeutic approaches based on agents which bind and neutralize LPS activities are particularly attractive because these substances directly block the primary stimulus for the entire proinflammatory cytokine cascade. Here we describe new activities of the LALF31–52 peptide, other than its LPS binding ability. Surprisingly, supernatants from human mononuclear cells stimulated with the LALF peptide are able to induce in vitro antiviral effects on the Hep-2 cell line mediated by gamma interferon (IFN-γ) and IFN-α. Analysis of the effect of LALF31–52 on tumor necrosis factor (TNF) and nitric oxide (NO) production by LPS-stimulated peritoneal macrophages revealed that a pretreatment with the peptide decreased LPS-induced TNF production but did not affect NO generation. This indicates that the LALF peptide modifies the LPS-induced response. In a model in mice with peritoneal fulminating sepsis, LALF31–52 protected the mice when administered prophylactically, and this effect is related to reduced systemic TNF-α levels. This study demonstrates, for the first time, the anti-inflammatory properties of the LALF-derived peptide. These properties widen the spectrum of the therapeutic potential for this LALF-derived peptide and the molecules derived from it. These agents may be useful in the prophylaxis and therapy of viral and bacterial infectious diseases, as well as for septic shock.

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