Direct in vitro organogenesis from sprouted seeds of a highly economical and ecological valued tree, Korean pine

Abstract The de novo organogenesis system for Korean pine of this economically and ecologically coniferous species was success fully established using sprouted seeds as the original explants. After 30 days of incubation, 92.67% of explants produced direct shoots on Gupta and Durzan(DCR) medium containing 2 mg · L −1 kinetin (KT) in combination 0.5 mg · L −1 thidiazuron (TDZ) with a maximum of about 15 shoots per explant respectively. We also confirmed the organogenic regeneration pattern by scanning electron microscopy (SEM) observation. For shoot elongation and growth after 60 days of culture, we obtained the highest mean length of 34.99 mm from DCR basal media supplemented with 6-benzyladenine (6-BA; 0.2 mg · L −1 ), 1-Naphthaleneacetic acid (NAA; 0.1 mg · L −1 ), and activated charcoal (AC; 1 g · L −1 ). The highest rooting percentages of 20.74-21.48% were observed within two months in the 1/2 DCR medium (major elements halved) enriched with 0.05 mg·L −1 NAA and 0.5 or 1 mg · L −1 indole-3-butyric acid (IBA). Rooted plants showed a survival rate of 90.28% in perlite: peat: vermiculite = 1:1:1 after acclimatization. This protocol is a successful and efficient biotechnological approach to the micropropagation of Korean pine, and these data will be helpful to the clonal propagation and conservation of Korean pine.

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Biochemical profile of cuttings used for in vitro organogenesis of Prunus africana: An endangered species in Cameroon

AFRICAN JOURNAL OF BIOTECHNOLOGY ◽

10.5897/ajb2014.14198 ◽

2015 ◽

Vol 14 (33) ◽

pp. 2568-2575

Author(s):

Germo Nzweundji Justine ◽

Niemenak Nicolas ◽

Oumar ◽

Judith Tsafack Julie ◽

Konan Koffi ◽

...

Keyword(s):

Endangered Species ◽

Biochemical Profile ◽

In Vitro Organogenesis ◽

Prunus Africana

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An efficient protocol for in vitro organogenesis and antioxidant studies in Melia dubia Cav.

AFRICAN JOURNAL OF BIOTECHNOLOGY ◽

10.5897/ajb2015.15153 ◽

2016 ◽

Vol 15 (19) ◽

pp. 768-775 ◽

Cited By ~ 3

Author(s):

Naseer Shah Syed ◽

Ahmad Wani Tareq ◽

Ram Bhimi ◽

Koul Monika ◽

Awasthi Praveen ◽

...

Keyword(s):

In Vitro Organogenesis ◽

Melia Dubia

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In vitro organogenesis and plant regeneration from unpollinated ovary cultures of Azadirachta indica

Biologia Plantarum ◽

10.1007/s10535-009-0067-2 ◽

2009 ◽

Vol 53 (2) ◽

pp. 360-364 ◽

Cited By ~ 13

Author(s):

P. Srivastava ◽

M. Singh ◽

P. Mathur ◽

R. Chaturvedi

Keyword(s):

Plant Regeneration ◽

Azadirachta Indica ◽

In Vitro Organogenesis

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Genetics of in vitro organogenesis and precocious germination of wheat embryos

Genetics and Molecular Biology ◽

10.1590/s1415-47571998000100015 ◽

1998 ◽

Vol 21 (1) ◽

pp. 87-92 ◽

Cited By ~ 2

Author(s):

Claudia E. Lange ◽

Luiz C. Federizzi ◽

Fernando I.F. Carvalho ◽

Ana L.C. Dornelles ◽

Cristine L. Handel

Keyword(s):

Gene Action ◽

Precocious Germination ◽

Genetic Determination ◽

Wheat Embryo ◽

In Vitro Organogenesis ◽

Generation Means ◽

Generation Means Analysis ◽

Wheat Embryos ◽

Segregating Population

The genetic bases of in vitro organogenesis and precocious germination of embryos in immature wheat embryo culture were investigated using six Brazilian genotypes and their F1, F2, BC1F1 and BC2F1 generations in a generation means analysis. Four parents and one set of F1’s were also analyzed in a diallel experiment. The results indicated a complex gene action controlling both traits, with additive, dominant and epistatic effects. High broad sense heritability values were found, indicating genetic determination. Considering the complexity of gene control, genetic gain could be achieved by selecting for the traits in advanced generations of the segregating population. Low correlation values between organogenesis, precocious germination, regeneration and somatic embryogenesis (data shown in a previous report) indicated the possibility of obtaining recombinant genotypes.

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