Antagonistic activity of lipopeptide-biosurfactant producing Bacillus subtilis AKP, against Colletotrichum capsici, the causal organism of anthracnose disease of chilli

2021 ◽  
Vol 36 ◽  
pp. 102133
Author(s):  
Amrit Kumar ◽  
Jintu Rabha ◽  
Dhruva Kumar Jha
Keyword(s):  
Bacillus Subtilis ◽  
Antagonistic Activity ◽  
Causal Organism ◽  
Anthracnose Disease ◽  
Colletotrichum Capsici ◽  
Lipopeptide Biosurfactant

Antagonistic activity of Bacillus subtilis strains isolated from various sources

2018 ◽  
Vol 8 (2) ◽  
pp. 354-364
Author(s):  
A. N. Irkitova ◽  
A. V. Grebenshchikova ◽  
A. V. Matsyura
Keyword(s):  
Escherichia Coli ◽  
Bacillus Subtilis ◽  
Wild Strain ◽  
Fish Farming ◽  
Antagonistic Activity ◽  
Antagonistic Effect ◽  
E Coli ◽  
Long Period ◽  
Test Culture ◽  
Agar Media

<p>An important link in solving the problem of healthy food is the intensification of the livestock, poultry and fish farming, which is possible only in the adoption and rigorous implementation of the concept of rational feeding of animals. In the implementation of this concept required is the application of probiotic preparations. Currently, there is an increased interest in spore probiotics. In many ways, this can be explained by the fact that they use no vegetative forms of the bacilli and their spores. This property provides spore probiotics a number of advantages: they are not whimsical, easily could be selected, cultivated, and dried. Moreover, they are resistant to various factors and could remain viable during a long period. One of the most famous spore microorganisms, which are widely used in agriculture, is <em>Bacillus subtilis</em>. Among the requirements imposed to probiotic microorganisms is mandatory – antagonistic activity to pathogenic and conditional-pathogenic microflora. The article presents the results of the analysis of antagonistic activity of collection strains of <em>B. subtilis</em>, and strains isolated from commercial preparations. We studied the antagonistic activity on agar and liquid nutrient medias to trigger different antagonism mechanisms of <em>B. subtilis</em>. On agar media, we applied three diffusion methods: perpendicular bands, agar blocks, agar wells. We also applied the method of co-incubating the test culture (<em>Escherichia coli</em>) and the antagonist (or its supernatant) in the nutrient broth. Our results demonstrated that all our explored strains of <em>B. subtilis</em> have antimicrobial activity against a wild strain of <em>E. coli</em>, but to varying degrees. We identified strains of <em>B. subtilis</em> with the highest antagonistic effect that can be recommended for inclusion in microbial preparations for agriculture.</p><p><em><br /></em><em></em></p>

scholarly journals Synchrotron-based FTIR microspectroscopy of chili resistance induced by Bacillus subtilis strain D604 against anthracnose disease

2017 ◽  
Vol 12 (1) ◽  
pp. 255-263 ◽  
Author(s):  
Kanjana Thumanu ◽  
Darawadee Wongchalee ◽  
Mathukorn Sompong ◽  
Piyaporn Phansak ◽  
Toan Le Thanh ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Subtilis Strain ◽  
Anthracnose Disease ◽  
Ftir Microspectroscopy

scholarly journals Antagonistic activity of Pseudomonas fluorescens isolates against Colletotrichum capsici

2019 ◽  
Vol 7 (4) ◽  
pp. 621-626
Author(s):  
Thaveedu, S. ◽  
Sutha Raja kumar, R., ◽  
Darwin Christdhas Henry, L. ◽  
Jaiganesh, V. ◽  
Kannan, C.
Keyword(s):  
Pseudomonas Fluorescens ◽  
Antagonistic Activity ◽  
Colletotrichum Capsici

scholarly journals Identification of the Fungal Pathogens of Postharvest Disease on Peach Fruits and the Control Mechanisms of Bacillus subtilis JK-14

Toxins ◽  
2019 ◽  
Vol 11 (6) ◽  
pp. 322 ◽  
Author(s):  
Shuwu Zhang ◽  
Qi Zheng ◽  
Bingliang Xu ◽  
Jia Liu
Keyword(s):  
Biological Control ◽  
Bacillus Subtilis ◽  
Fungal Pathogens ◽  
Biological Control Agent ◽  
Storage Period ◽  
Control Agent ◽  
Antagonistic Activity ◽  
Postharvest Disease ◽  
Economic Losses ◽  
Control Mechanisms

Postharvest fungal disease is one of the significant factors that limits the storage period and marketing life of peaches, and even result in serious economic losses worldwide. Biological control using microbial antagonists has been explored as an alternative approach for the management of postharvest disease of fruits. However, there is little information available regarding to the identification the fungal pathogen species that cause the postharvest peach diseases and the potential and mechanisms of using the Bacillus subtilis JK-14 to control postharvest peach diseases. In the present study, a total of six fungal isolates were isolated from peach fruits, and the isolates of Alternaria tenuis and Botrytis cinerea exhibited the highest pathogenicity and virulence on the host of mature peaches. In the culture plates, the strain of B. subtilis JK-14 showed the significant antagonistic activity against the growth of A. tenuis and B. cinerea with the inhibitory rates of 81.32% and 83.45% at 5 days after incubation, respectively. Peach fruits treated with different formulations of B. subtilis JK-14 significantly reduced the mean disease incidences and lesion diameters of A. tenuis and B. cinerea. The greatest mean percent reduction of the disease incidences (81.99% and 71.34%) and lesion diameters (82.80% and 73.57%) of A. tenuis and B. cinerea were obtained at the concentration of 1 × 107 CFU mL−1 (colony forming unit, CFU). Treatment with the strain of B. subtilis JK-14 effectively enhanced the activity of the antioxidant enzymes-superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) in A. tenuis and B. cinerea inoculated peach fruits. As such, the average activities of SOD, POD and CAT were increased by 36.56%, 17.63% and 20.35%, respectively, compared to the sterile water treatment. Our results indicate that the isolates of A. tenuis and B. cinerea are the main pathogens that cause the postharvest peach diseases, and the strain of B. subtilis JK-14 can be considered as an environmentally-safe biological control agent for the management of postharvest fruits diseases. We propose the possible mechanisms of the strain of B. subtilis JK-14 in controlling of postharvest peach diseases.

Evaluation of Bacillus subtilis SPB1 Lipopeptide Biosurfactant Toxicity Towards Mice

2014 ◽  
Vol 20 (3) ◽  
pp. 333-340 ◽  
Author(s):  
Rihab Sahnoun ◽  
Inès Mnif ◽  
Hammadi Fetoui ◽  
Radhouan Gdoura ◽  
Khansa Chaabouni ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Lipopeptide Biosurfactant

Characterization of an antifungal soil bacterium and its antagonistic activities againstFusariumspecies

2003 ◽  
Vol 49 (4) ◽  
pp. 253-262 ◽  
Author(s):  
Yiu-Kwok Chan ◽  
Wayne A McCormick ◽  
Keith A Seifert
Keyword(s):  
Bacillus Subtilis ◽  
Culture Filtrate ◽  
Fusarium Species ◽  
Hyphal Growth ◽  
Antagonistic Activity ◽  
Plant Diseases ◽  
Ear Rot ◽  
In Planta ◽  
Head Blight

Bacteria were isolated from a cultivated soil and screened for antagonistic activity against Fusarium graminearum, a predominant agent of ear rot and head blight in cereal crops. Based on its in vitro effectiveness, isolate D1/2 was selected for characterization and identified as a strain of Bacillus subtilis by phenotypic tests and comparative analysis of its 16S ribosomal RNA gene (rDNA) sequence. It inhibited the mycelial growth of a collection of common fungal phytopathogens, including eight Fusarium species, three other ascomycetes, and one basidiomycete. The cell-free culture filtrate of D1/2 at different dilutions was active against macroconidium germination and hyphal growth of F. graminearum, depending on the initial macroconidium density. It induced the formation of swollen hyphal cells in liquid cultures of this fungus grown from macroconidia. A bioassay also demonstrated that D1/2 offered in planta protection against the damping-off disease in alfalfa seedlings caused by F. graminearum, while the type strain of B. subtilis was ineffective. Hence, B. subtilis D1/2 or its culture filtrate has potential application in controlling plant diseases caused by Fusarium.Key words: antifungal activity, Bacillus subtilis, biological control, biopesticide, Fusarium species.

scholarly journals Management of Anthracnose Disease of Aloe vera in Bangladesh

2021 ◽  
pp. 7-15
Author(s):  
Abu Noman Faruq Ahmmed ◽  
Md. Al-Amin Islam ◽  
Fatema Begum ◽  
Salma Sarker
Keyword(s):  
Disease Severity ◽  
Field Experiments ◽  
Bordeaux Mixture ◽  
Block Design ◽  
Aloe Vera ◽  
Pathogenicity Test ◽  
Traditional Use ◽  
Causal Organism ◽  
Anthracnose Disease ◽  
Garlic Bulb

Aims: To identify the causal organisms of anthracnose disease of Aloe vera in Bangladesh and to manage this disease in field condition. Study Design: The experiment was designed by Randomized Complete Block Design (RCBD) with three replications. Place and Duration of Study: The field experiments were conducted in Natore, Bangladesh and the laboratory experiments were carried out at the Department of Plant Pathology, Sher-e-Bangla Agricultural University, Dhaka, Bangladesh from January, 2017 to December, 2018. Methodology: The causal organism Colletotrichum gloeosporioides was isolated by tissue plating method and identified based on morphological and cultural characteristics and that was confirmed by pathogenicity test. Infested farmer’s fields were selected in rainy season under natural epiphytic condition to evaluate the efficacy of eleven treatments. Results: Among the treatments, Bordeaux mixture gave best result against this disease. Moreover, Tilt 250 EC and Folicur 25 EC and Garlic bulb extract showed better effect against the disease than the other treatments. Lime also has moderate effect against anthracnose disease of A. vera. In 2017, after 4th spray, the lowest incidence was recorded in Bordeaux mixture (58.33%) which was statistically identical with Folicur (64.58%), Tilt (64.58%) and Garlic bulb extract (66.67%). Similarly, the lowest disease severity was found in Bordeaux mixture (3.55) followed by Folicur (5.67%), Tilt (6.67%) and Garlic bulb extract (7.67%). Similar result also found in 2018. After 4th spray, the lowest incidence was recorded in Bordeaux mixture (38.58%) which was statistically identical with Lime (41.66%) and Garlic bulb extract (45.83%). Similarly, the lowest disease severity was found in Bordeaux mixture (0.20%) followed by Lime (0.25%) and Garlic bulb extract (0.36%). Conclusion: Garlic bulb extract could be used as eco-friendly approach. Moreover, use of Bordeaux mixture is better than the traditional use of lime. From chemical pesticides, Tilt 250 EC and Folicur 25 EC could be used for controlling the disease as the last option.

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