A high throughput single-stranded conformation polymorphism assay for detection, subtyping and genotyping of influenza viruses

2004 ◽  
Vol 1263 ◽  
pp. 653-657 ◽  
Author(s):  
Joseph D Quinto ◽  
Chiaoyin Kathy Wang
Keyword(s):  
High Throughput ◽  
Influenza Viruses ◽  
Single Stranded Conformation Polymorphism ◽  
Conformation Polymorphism

scholarly journals Asymmetric synthesis of host-directed inhibitors of myxoviruses

2013 ◽  
Vol 9 ◽  
pp. 197-203 ◽  
Author(s):  
Terry W Moore ◽  
Kasinath Sana ◽  
Dan Yan ◽  
Pahk Thepchatri ◽  
John M Ndungu ◽  
...  
Keyword(s):  
Antiviral Activity ◽  
Asymmetric Synthesis ◽  
High Throughput ◽  
High Throughput Screening ◽  
Aqueous Solubility ◽  
Influenza Viruses ◽  
Lead Compound ◽  
Synthetic Strategy ◽  
Aqueous Solubilities ◽  
Nanomolar Range

High-throughput screening (HTS) previously identified benzimidazole 1 (JMN3-003) as a compound with broad antiviral activity against different influenza viruses and paramyxovirus strains. In pursuit of a lead compound from this series for development, we sought to increase both the potency and the aqueous solubility of 1. Lead optimization has achieved compounds with potent antiviral activity against a panel of myxovirus family members (EC50 values in the low nanomolar range) and much improved aqueous solubilities relative to that of 1. Additionally, we have devised a robust synthetic strategy for preparing 1 and congeners in an enantio-enriched fashion, which has allowed us to demonstrate that the (S)-enantiomers are generally 7- to 110-fold more potent than the corresponding (R)-isomers.

scholarly journals Genotyping of human parvovirus B19 in clinical samples from Brazil and Paraguay using heteroduplex mobility assay, single-stranded conformation polymorphism and nucleotide sequencing

2011 ◽  
Vol 106 (4) ◽  
pp. 502-504 ◽  
Author(s):  
Marcos César Lima de Mendonça ◽  
Ana Maria de Amorim Ferreira ◽  
Marta Gonçalves Matos dos Santos ◽  
Elva Cristina Oviedo ◽  
Maria Sônia Dal Bello ◽  
...  
Keyword(s):  
Parvovirus B19 ◽  
Clinical Samples ◽  
Nucleotide Sequencing ◽  
Human Parvovirus B19 ◽  
Single Stranded Conformation Polymorphism ◽  
Heteroduplex Mobility Assay ◽  
Conformation Polymorphism

scholarly journals Subtyping of Swine Influenza Viruses Using a High-Throughput Real-Time PCR Platform

2018 ◽  
Vol 8 ◽  
Author(s):  
Nicole B. Goecke ◽  
Jesper S. Krog ◽  
Charlotte K. Hjulsager ◽  
Kerstin Skovgaard ◽  
Timm C. Harder ◽  
...  
Keyword(s):  
Real Time ◽  
High Throughput ◽  
Real Time Pcr ◽  
Swine Influenza ◽  
Influenza Viruses
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