Description of a serpin toxin in Loxosceles (Brown spider) venoms: Cloning, expression in baculovirus-infected insect cells and functional characterization

2021 ◽  
Author(s):  
Zelinda Schemczssen-Graeff ◽  
Hanna Câmara da Justa ◽  
Jenifer Nowatzki ◽  
Antonielle Beatriz Baldissera ◽  
Nayanne Louise Costacurta Polli ◽  
...  
Keyword(s):  
Insect Cells ◽  
Functional Characterization ◽  
Infected Insect ◽  
Brown Spider

scholarly journals Purification and functional characterization of the human β2 -adrenergic receptor produced in baculovirus-infected insect cells

FEBS Letters ◽  
1991 ◽  
Vol 282 (2) ◽  
pp. 441-444 ◽  
Author(s):  
Helmut Reiländer ◽  
Fritz Boege ◽  
Subhash Vasudevan ◽  
Gabi Maul ◽  
Mirko Hekman ◽  
...  
Keyword(s):  
Adrenergic Receptor ◽  
Insect Cells ◽  
Functional Characterization ◽  
Infected Insect ◽  
Β2 Adrenergic Receptor

Production of a novel recombinant brown spider hyaluronidase in baculovirus-infected insect cells

2021 ◽  
pp. 109759
Author(s):  
Elidiana De-Bona ◽  
Daniele Chaves-Moreira ◽  
Thiago Beltrami Dias Batista ◽  
Hanna Câmara da Justa ◽  
Gustavo Rodrigues Rossi ◽  
...  
Keyword(s):  
Insect Cells ◽  
Infected Insect ◽  
Brown Spider

scholarly journals Production, processing and partial purification of functional G protein βγ subunits in baculovirus-infected insect cells

1992 ◽  
Vol 286 (3) ◽  
pp. 677-680 ◽  
Author(s):  
J D Robishaw ◽  
V K Kalman ◽  
K L Proulx
Keyword(s):  
G Protein ◽  
G Proteins ◽  
Insect Cells ◽  
Expression System ◽  
Baculovirus Expression System ◽  
Partial Purification ◽  
Baculovirus Expression ◽  
Gamma Subunits ◽  
Infected Insect ◽  
Beta 2

As a result of the inability to resolve the heterogeneous mixture of G protein beta gamma subunits present in tissues, it has not been possible to compare different beta gamma subunits of the G proteins in terms of their proposed roles in receptor-effector coupling. This study was undertaken to establish the utility of the baculovirus expression system in producing homogeneous beta gamma subunits of defined composition for the comparative analysis of these subunits in reconstitution systems. In this study we report the expression, and appropriate post-translational processing, of recombinant beta 2, gamma 2 and gamma 3 subunits. In addition, we show that the recombinant beta gamma subunits can be readily purified, and can functionally interact with the alpha subunits of the G proteins.

Expression and Characterization of Human Dopamine D2Receptor in Baculovirus-Infected Insect Cells

1994 ◽  
Vol 14 (2) ◽  
pp. 99-117 ◽  
Author(s):  
J. A. Javitch ◽  
J. Kaback ◽  
X. Li ◽  
A. Karlin
Keyword(s):  
Insect Cells ◽  
Infected Insect

Expression of murine soluble CD4 protein in baculovirus infected insect cells

Immunobiology ◽  
1992 ◽  
Vol 186 (3-4) ◽  
pp. 254-267 ◽  
Author(s):  
Jörg Kupsch ◽  
Kai M. Saizawa ◽  
Klaus Eichmann
Keyword(s):  
Insect Cells ◽  
Infected Insect ◽  
Soluble Cd4
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