SIRT2 Contributes to the Regulation of Intestinal Cell Proliferation and Differentiation

The past years have witnessed an increasing number of reports relative to homeobox genes in endoderm-derived tissues. In this review, we focus on the caudal-related Cdx-1 and Cdx-2 homeobox genes to give an overview of the in vivo, in vitro, and ex vivo approaches that emphasize their primary role in intestinal development and in the control of intestinal cell proliferation, differentiation, and identity. The participation of these genes in colon tumorigenesis and their identification as important actors of the oncogenic process are also discussed.Key words: caudal, epithelial cell proliferation and differentiation, cancer.

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The in ovo injection of methionine improves intestinal cell proliferation and differentiation in chick embryos by activating the JAK2/STAT3 signaling pathway

Animal Nutrition ◽

10.1016/j.aninu.2021.03.009 ◽

2021 ◽

Author(s):

Meng-Jie Chen ◽

Jia-Yi Zhou ◽

Yi-Jun Chen ◽

Xiu-Qi Wang ◽

Hui-Chao Yan ◽

...

Keyword(s):

Cell Proliferation ◽

Signaling Pathway ◽

Intestinal Cell ◽

Chick Embryos ◽

In Ovo ◽

Stat3 Signaling ◽

Cell Proliferation And Differentiation ◽

Stat3 Signaling Pathway ◽

Proliferation And Differentiation ◽

In Ovo Injection

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Biological activities of commercial bovine lactoferrin sources

Biochemistry and Cell Biology ◽

10.1139/bcb-2020-0182 ◽

2020 ◽

Author(s):

Bo Lönnerdal ◽

Xiaogu Du ◽

Rulan Jiang

Keyword(s):

Cell Proliferation ◽

Biological Activities ◽

Intestinal Cell ◽

Bovine Lactoferrin ◽

Cell Proliferation And Differentiation ◽

Sds Page ◽

Proliferation And Differentiation ◽

Intestinal Proliferation ◽

Tgf Β1

Lactoferrin (Lf) samples from several manufacturers were evaluated in vitro. The purity and protein form of each Lf were examined by SDS-PAGE, Western blot, and proteomics analysis. Assays were conducted to evaluate uptake of Lfs and iron from Lfs by enterocytes as well as Lf bioactivities, including effects on intestinal cell proliferation and differentiation, IL-18 secretion, TGF-β1 transcription, and growth of enteropathogenic Escherichia coli (EPEC). Composition of the Lfs varied; some only contain a major Lf band (~80 kDa), and some also contain minor forms. All Lfs and iron from the Lfs were absorbed by Caco-2 cells with varying efficiencies. The bioactivities of the Lfs varied considerably, but there was no consistent trend. All Lfs promoted intestinal cell proliferation, secretion of IL-18, and transcription of TGF-β1. Some Lfs exhibited pro-differentiation effects on Caco-2 cells. Effects of pasteurization (62.5°C for 30 min, 72°C for 15 sec, or 121°C for 5 min) on integrity, uptake and bioactivities were examined using Dicofarm, Tatua, and native bovine Lfs. Results show that pasteurization did not affect protein integrity, but variously affected uptake of Lf, and its effects on intestinal proliferation, differentiation, and EPEC growth. To choose a Lf source for a clinical trial, assessment of bioactivities is recommended.

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85 – Sirt2 Regulates Intestinal Cell Proliferation and Differentiation

Gastroenterology ◽

10.1016/s0016-5085(19)36819-2 ◽

2019 ◽

Vol 156 (6) ◽

pp. S-19

Author(s):

Yuning Zhou ◽

Chang Li ◽

Piotr Rychahou ◽

Heidi L. Weiss ◽

Eun Y. Lee ◽

...

Keyword(s):

Cell Proliferation ◽

Intestinal Cell ◽

Cell Proliferation And Differentiation ◽

Proliferation And Differentiation

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Faculty Opinions recommendation of Adhesion forces and cortical tension couple cell proliferation and differentiation to drive epidermal stratification.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.732284718.793547053 ◽

2018 ◽

Author(s):

Pascal Silberzan

Keyword(s):

Cell Proliferation ◽

Adhesion Forces ◽

Cortical Tension ◽

Cell Proliferation And Differentiation ◽

Proliferation And Differentiation

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Icariin Stimulates hFOB 1.19 Osteoblast Proliferation and Differentiation via OPG/RANKL Mediated by the Estrogen Receptor

Current Pharmaceutical Biotechnology ◽

10.2174/1389201021666200123102550 ◽

2020 ◽

Vol 22 (1) ◽

pp. 168-175 ◽

Cited By ~ 1

Author(s):

Lin-Jun Sun ◽

Chong Li ◽

Xiang-hao Wen ◽

Lu Guo ◽

Zi-Fen Guo ◽

...

Keyword(s):

Cell Proliferation ◽

Estrogen Receptor ◽

Protein Expression ◽

Chinese Herb ◽

Human Osteoblast ◽

Osteoblast Cells ◽

Expression Ratio ◽

Cell Proliferation And Differentiation ◽

Proliferation And Differentiation ◽

Mrna And Protein Expression

Background:: Icariin (ICA), one of the main effective components isolated from the traditional Chinese herb Epimedium brevicornu Maxim., has been reported to possess extensive pharmacological actions, including enhanced sexual function, immune regulation, anti-inflammation, and antiosteoporosis. Methods:: Our study was designed to investigate the effect of ICA on cell proliferation and differentiation and the molecular mechanism of OPG/RANKL mediated by the Estrogen Receptor (ER) in hFOB1.19 human osteoblast cells. Results:: The experimental results show that ICA can stimulate cell proliferation and increase the activity of Alkaline Phosphatase (ALP), Osteocalcin (BGP) and I Collagen (Col I) and a number of calcified nodules. Furthermore, the mRNA and protein expression of OPG and RANKL and the OPG/ RANKL mRNA and protein expression ratios were upregulated by ICA. The above-mentioned results indicated that the optimal concentration of ICA for stimulating osteogenesis was 50ng/mL. Subsequent mechanistic studies comparing 50ng/mL ICA with an estrogen receptor antagonist demonstrated that the effect of the upregulated expression is connected with the estrogen receptor. In conclusion, ICA can regulate bone formation by promoting cell proliferation and differentiation and upregulating the OPG/RANKL expression ratio by the ER in hFOB1.19 human osteoblast cells.

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Costimulation by B7 Modulates Specificity of Cytotoxic T Lymphocytes: A Missing Link That Explains Some Bystander T Cell Activation

Journal of Experimental Medicine ◽

10.1084/jem.186.10.1787 ◽

1997 ◽

Vol 186 (10) ◽

pp. 1787-1791 ◽

Cited By ~ 10

Author(s):

Pan Zheng ◽

Yang Liu

Keyword(s):

Cell Proliferation ◽

T Cells ◽

Influenza Virus ◽

T Cell ◽

T Cell Activation ◽

Cell Activation ◽

T Cell Proliferation ◽

Target Cells ◽

Cell Proliferation And Differentiation ◽

Proliferation And Differentiation

It has been proposed that some bystander T cell activation may in fact be due to T cell antigen receptor (TCR) cross-reactivity that is too low to be detected by the effector cytotoxic T lymphocyte (CTL). However, this hypothesis is not supported by direct evidence since no TCR ligand is known to induce T cell proliferation and differentiation without being recognized by the effector CTL. Here we report that transgenic T cells expressing a T cell receptor to influenza virus A/NT/68 nucleoprotein (NP) 366-374:Db complexes clonally expand and become effector CTLs in response to homologous peptides from either A/PR8/34 (H1N1), A/AA/60 (H2N2), or A/NT/68 (H3N2). However, the effector T cells induced by each of the three peptides kill target cells pulsed with NP peptides from the H3N2 and H2N2 viruses, but not from the H1N1 virus. Thus, NP366–374 from influenza virus H1N1 is the first TCR ligand that can induce T cell proliferation and differentiation without being recognized by CTLs. Since induction of T cell proliferation was mediated by antigen-presenting cells that express costimulatory molecules such as B7, we investigated if cytolysis of H1N1 NP peptide–pulsed targets can be restored by expressing B7-1 on the target cells. Our results revealed that this is the case. These data demonstrated that costimulatory molecule B7 modulates antigen specificity of CTLs, and provides a missing link that explains some of the bystander T cell activation.

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