scholarly journals Interactions between Salmonella Enteritidis and food processing facility isolate Bacillus paramycoides B5 in dual-species biofilms

LWT ◽  
2022 ◽  
pp. 113053
Author(s):  
Jing-Guo Xu ◽  
Hui-Xue Hu ◽  
Bei-Zhong Han ◽  
Jing-Yu Chen
Keyword(s):  
Food Processing ◽  
Salmonella Enteritidis ◽  
Processing Facility

scholarly journals Persistent Listeria monocytogenes Isolates from a Poultry-Processing Facility Form More Biofilm but Do Not Have a Greater Resistance to Disinfectants than Sporadic Strains

Pathogens ◽  
2019 ◽  
Vol 8 (4) ◽  
pp. 250 ◽  
Author(s):  
Daniel Rodríguez-Campos ◽  
Cristina Rodríguez-Melcón ◽  
Carlos Alonso-Calleja ◽  
Rosa Capita
Keyword(s):  
Listeria Monocytogenes ◽  
Food Processing ◽  
Biofilm Formation ◽  
Benzalkonium Chloride ◽  
Bacterial Persistence ◽  
Crystal Violet Assay ◽  
Processing Facility ◽  
Poultry Processing ◽  
Sessile Cells ◽  
Selection Of

Some strains of Listeria monocytogenes can persist in food-processing environments, increasing the likelihood of the contamination of foodstuffs. To identify traits that contribute to bacterial persistence, a selection of persistent and sporadic L. monocytogenes isolates from a poultry-processing facility was investigated for biofilm-forming ability (crystal violet assay). The susceptibility of sessile cells to treatments (five minutes) with sodium hypochlorite having 10% active chlorine (SHY: 10,000 ppm, 25,000 ppm, and 50,000 ppm) and benzalkonium chloride (BZK: 2500 ppm, 10,000 ppm, and 25,000 ppm) was also studied. All isolates exhibited biofilm formation on polystyrene. Persistent strains showed larger (p < 0.001) biofilm formation (OD580 = 0.301 ± 0.097) than sporadic strains (OD580 = 0.188 ± 0.082). A greater susceptibility to disinfectants was observed for biofilms of persistent strains than for those of sporadic strains. The application of SHY reduced biofilms only for persistent strains. BZK increased OD580 in persistent strains (2500 ppm) and in sporadic strains (all concentrations). These results indicate that the use of BZK at the concentrations tested could represent a public health risk. Findings in this work suggest a link between persistence and biofilm formation, but do not support a relationship between persistence and the resistance of sessile cells to disinfectants.

scholarly journals Microbes in Our Food, an Ongoing Problem with New Solutions

Antibiotics ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 584
Author(s):  
Birgit M. Prüβ
Keyword(s):  
Food Processing ◽  
Microbial Contamination ◽  
Bacterial Resistance ◽  
Disease Outbreaks ◽  
Basic Research ◽  
The United States ◽  
Bacterial Biofilms ◽  
Special Issue ◽  
Food Borne ◽  
Processing Facility

Despite an increasing number of techniques that are designed to mitigate microbial contamination of food and the resulting food borne disease outbreaks, the United States and many other countries across the world continue to experience impressive numbers of such outbreaks. Microbial contamination can occur during activities that take place in the pre-harvest environment or in the processing facility post-harvest. Current treatments of food that are aimed at reducing bacterial numbers may be only partially effective because of the development of bacterial resistance, the formation of bacterial biofilms, and inactivation of the treatment compound by the food products themselves. This Special Issue will include basic research approaches that are aimed at enhancing our understanding of how contamination occurs throughout the food processing chain, as well as more immediate and applied approaches to the development and use of novel anti-microbials to combat microbes in food. Novel techniques that aim to evaluate the efficacy of novel anti-microbials are included. Overall, we present a broad spectrum of novel approaches to reduce microbial contamination on food at all stages of production.

The microbiome of water and water-associated biofilms in meat processing facilities

2020 ◽  
Author(s):  
Eva M. Wagner ◽  
Sarah Thalguter ◽  
Kathrin Rychli ◽  
Martin Wagner
Keyword(s):  
Food Processing ◽  
Extracellular Dna ◽  
Environmental Research ◽  
Meat Processing ◽  
Food Contact ◽  
Wide Range ◽  
Processing Facility ◽  
Matrix Components ◽  
Biofilm Development ◽  
Food Processing Environment

&lt;p&gt;Drinking water represents a reservoir for microorganisms. Microorganisms from water are able to attach to the inner surface of a water hose and nourish themselves by the leaking carbon molecules. Through the interaction of different species a multi-species biofilm can develop. Especially in the food processing environment biofilms in water hoses represent a risk factor. Within the food processing sector water hoses are often used to remove disinfecting agents from freshly cleaned surfaces, after the cleaning and disinfection procedure. When biofilms are located inside these water hoses, cells or cell clusters can detach, subsequently contaminating cleaned food contact surfaces.&lt;/p&gt; &lt;p&gt;We checked water hoses as a biofilm hotspot in a meat processing facility by using a flocked swab for biofilm sampling inside the water hose and accessory parts (i.e. nozzle). The bacterial load (culture-based and DNA-based) and the presence of matrix components (carbohydrates, proteins and extracellular DNA) were analysed. &amp;#160;&lt;/p&gt; &lt;p&gt;Herby we identified three from six tested water hoses to harbour a biofilm, by being positive for microorganisms and at least two matrix components. This clearly states the need for further understanding of biofilm formation in water hoses. Within the three other hoses, microorganisms could be detected, but no matrix components. We could isolate twelve genera of the water hose associated biofilms using one growth medium (TSA) and two different incubation temperatures (10&amp;#160;&amp;#176;C and 20&amp;#160;&amp;#176;C). There was only on genus that was present within all three water hose biofilms, which was &lt;em&gt;Rhodococcus&lt;/em&gt;. Previously this genus was isolated from a shower head (Lee 2013), and is known to catabolise a wide range of organic compounds. This potentially enables the growth in a nutrient poor environment like the water hose providing secondary colonisers launch aid to contribute to the biofilm. The genera &lt;em&gt;Flavobacterium&lt;/em&gt;, &lt;em&gt;Microbacterium&lt;/em&gt; and Stenotrophomonas were shared among two of the water hose biofilms. Experiments to assess the biofilm forming ability of &lt;em&gt;isolates&lt;/em&gt; of these genera using a mono-species static biofilm model indicate that all three species are able to produce matrix and can therefore be regarded as biofilm producers.&lt;/p&gt; &lt;p&gt;To date, there is limited information about biofilm development and presence in water hoses, especially in the food processing environment. This first identification of biofilms in water hoses and associated parts emphasizes the need of further research on this topic and detailed monitoring at these sites to prevent recontamination. A currently ongoing microbiome study on the water, the used water hoses, and the water-contacting food contact sites in a meat processing facility will give further details about the biofilm presence and possible transmission of microorganisms encountered there.&lt;/p&gt; &lt;p&gt;&amp;#160;&lt;/p&gt; &lt;p&gt;References:&lt;/p&gt; &lt;p&gt;Lee, Yoonjin. 2013. &amp;#8220;An Evaluation of Microbial and Chemical Contamination Sources Related to the Deterioration of Tap Water Quality in the Household Water Supply System.&amp;#8221; International Journal of Environmental Research and Public Health 10(9):4143&amp;#8211;60.&lt;/p&gt;

scholarly journals Implementation of a cogeneration plant for a food processing facility. A case study

2016 ◽  
Vol 102 ◽  
pp. 500-512 ◽  
Author(s):  
Vincenzo Bianco ◽  
Mattia De Rosa ◽  
Federico Scarpa ◽  
Luca A. Tagliafico
Keyword(s):  
Food Processing ◽  
Cogeneration Plant ◽  
Processing Facility

Effect of Time, Temperature and Transport Media on the Recovery of Listeria monocytogenes from Environmental Swabs

2020 ◽  
Author(s):  
Diana Stewart ◽  
Yadwinder Singh Rana ◽  
Kaiping Deng ◽  
Geethaanjali Vijayakumar ◽  
Lanlan Yin ◽  
...  
Keyword(s):  
Stainless Steel ◽  
Logistic Regression ◽  
Listeria Monocytogenes ◽  
Sodium Hypochlorite ◽  
Food Processing ◽  
Storage Time ◽  
Cheese Whey ◽  
Storage Temperature ◽  
Food Matrix ◽  
Processing Facility

Environmental monitoring for Listeria monocytogenes in food processing environments is key for ensuring the safety of ready-to-eat foods. For sampling, swabs are often hydrated with a wetting or transport medium which may contain neutralizers and other ingredients. After swabbing the environment, the swabs may then be transported or shipped cold to an off-site laboratory for testing, ideally within 48 h. Extended shipping times may subject the pathogen to increased temperatures in the presence of the wetting medium, organics, and other chemicals from the processing facility which may confound detection. This study evaluated growth and detection of L. monocytogenes on stainless steel exposed to either buffer or sodium hypochlorite prior to drying. Swabs were rehydrated with Butterfield’s Phosphate Buffer, Neutralizing Buffer, Letheen Broth or Dey-Engley Neutralizing Broth prior to swabbing. Swabs were stored in the presence of no added food, cheese whey or ice cream under both optimal (4°) and sub-optimal (15°C) temperatures for up to 72 h. Overall, there was no growth of L. monocytogenes at 4°C through 72 h storage, though enrichment from these swabs was dependent on the presence and type of food matrix. Pathogen growth during storage at 15°C was more variable and depended on both the food matrix and transport media used, with Dey-Engley and Letheen Broth allowing for the highest population increases. Overall, more enrichments resulting in L. monocytogenes detections were observed when using Letheen Broth and Neutralizing Buffer than Dey-Engley which resulted in fewer detections at 15°C. Logistic regression and Cochran-Mantel-Haenszel (CMH) analyses determined that storage temperature, transport media, and food matrix all significantly affected detection of L. monocytogenes , while storage time did not have a clear effect on recovery from swabs.

Salmonella: A Postmodern Pathogen1

1991 ◽  
Vol 54 (7) ◽  
pp. 563-568 ◽  
Author(s):  
ROBERT V. TAUXE
Keyword(s):  
Public Health ◽  
Food Processing ◽  
Salmonella Enteritidis ◽  
Immunocompromised Patient ◽  
The United States ◽  
Salmonella Infections ◽  
Immunodeficiency Virus ◽  
The Future ◽  
Recent Trends ◽  
Animal Reservoirs

The reported incidence of Salmonella infections in the United States has increased substantially since reporting began in 1943. These infections cause important morbidity, mortality, and economic burden in this country and are particularly severe in the infant, elderly, or immunocompromised patient. Four recent trends suggest that salmonellosis will present an increasing challenge to public health in the future. Antimicrobial resistance is present in an increasing proportion of Salmonella isolates. Salmonella bacteremia has emerged as a serious complication of human immunodeficiency virus infection. Infections caused by the egg-associated serotype Salmonella enteritidis are steadily increasing in incidence and geographic scope, and these infections are now the most common form of salmonellosis in some parts of the country. Finally, contamination of food produced in centralized facilities has led to extremely large and widespread outbreaks. Better understanding of the biology of specific animal reservoirs and of the microbiologic aspects of food processing is needed to control salmonellosis in the future.

scholarly journals Recovering Heat from Condenser Unit Produced Refrigerant System in Food Processing Facility

2019 ◽  
Vol 63 (3) ◽  
pp. 220-229
Author(s):  
Miklós Kassai
Keyword(s):  
Food Processing ◽  
Energy Performance ◽  
Hot Water ◽  
Heat Utilization ◽  
Air Heating ◽  
Payback Time ◽  
Technical Realization ◽  
Processing Facility

The object of this research was to investigate of the energy performance of a national chicken slaughterhouse in order to achieve condenser heat utilization generated by the operation of the existing, refrigerant systems. The paper focuses also for the technical realization of condenser heat utilization by concept plans and payback time calculations. The utilizing heat of a cooling circle's condenser is a quite quickly returnable investment. The time of payback is around 330 days by heat utilizing for hot water preheating and also air heating.

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