Antioxidant and antibacterial study of 10 flavonoids revealed rutin as a potential antibiofilm agent in Klebsiella pneumoniae strains isolated from hospitalized patients

Abstract Background: Klebsiella pneumoniae is one of the leading causes of hospital outbreaks worldwide. Also, antibiotic-resistant K. pneumoniae is progressively being involved in invasive infections with high morbidity and mortality. The aim of the current study was to determine antimicrobial susceptibility patterns and the incidences of resistance genes (integron types and β-lactamase-encoded genes) among clinical isolates of K. pneumoniae. Methods: In this cross-sectional study, a total of 100 clinical samples were obtained from hospitalized patients in three teaching hospitals in the north of Iran, from November 2018 and October 2019. Antimicrobial susceptibility testing was performed using disk agar diffusion test in line with CLSI recommendation. For colistin, minimum inhibitory concentration (MIC) was determined using broth microdilution. Based on antibiogram, multi-drug resistant (MDR) and extensive-drug resistant (XDR) strains were detected. Finally, integron types and β-lactamase resistance genes were identified using polymerase chain reaction technique.Results. The most and least clinical samples were related to the urine and bronchoalveolar lavage, respectively. Based on the antibiogram results, amikacin and gentamicin exhibited good activity against K. pneumoniae strains in vitro. High resistance rate (93%) to ampicillin/sulbactam also predict the limited efficacy of this antibiotic. Among all the 100 isolates, the frequency of MDR and XDR strains were 58% and 13%, respectively, while no pan-drug resistant (PDR) isolates were found. The prevalence of blaSHV, blaTEM, blaCTX-M-15, blaKPC, blaOXA-48, blaNDM β-lactamase genes were 91.4%, 82.7%, 79.3%, 29.3%, 36.2% and 6.9%, respectively, however 58% of the isolates were carrying intI gene. Class II and III integrons were not detected in any isolates. Conclusion: The MDR K. pneumoniae is becoming a serious problem in hospitals, with many strains developing resistance to most available antimicrobials. Our results indicate co-presence of a series of β-lactamase and integron types on the MDR strains recovered from hospitalized patients. The increasing rate of these isolates emphasizes the importance of choosing an appropriate antimicrobial regimen based on antibiotic susceptibility pattern.

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Molecular characterization of extended-spectrum-β-lactamases producing Klebsiella pneumoniae and Escherichia coli from hospitalized patients in Oman

BMC Proceedings ◽

10.1186/1753-6561-3-s3-o10 ◽

2009 ◽

Vol 3 (Suppl 3) ◽

pp. O10 ◽

Cited By ~ 1

Author(s):

Lobna Mourad ◽

Matilda Nicklasson ◽

Rania Siam ◽

Guillermo Pimentel

Keyword(s):

Escherichia Coli ◽

Klebsiella Pneumoniae ◽

Molecular Characterization ◽

Hospitalized Patients ◽

Extended Spectrum

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Emerge of bla NDM-1 and bla OXA-48-like harboring carbapenem-resistant Klebsiella pneumoniae isolates from hospitalized patients in southwestern Iran

Journal of the Chinese Medical Association ◽

10.1016/j.jcma.2017.08.015 ◽

2018 ◽

Vol 81 (6) ◽

pp. 536-540 ◽

Cited By ~ 12

Author(s):

Zahra Hosseinzadeh ◽

Hadi Sedigh Ebrahim-Saraie ◽

Jamal Sarvari ◽

Jalal Mardaneh ◽

Behzad Dehghani ◽

...

Keyword(s):

Klebsiella Pneumoniae ◽

Hospitalized Patients ◽

Carbapenem Resistant ◽

Southwestern Iran

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Prevalence of Quinolone Resistance Genes in Klebsiella pneumoniae Strains Isolated from Hospitalized Patients During 2013 - 2014

Archives of Pediatric Infectious Diseases ◽

10.5812/pedinfect.38343 ◽

2016 ◽

Vol Inpress (Inpress) ◽

Cited By ~ 1

Author(s):

Mohsen Heidary ◽

Hossein Goudarzi ◽

Ali Hashemi ◽

Gita Eslami ◽

Mehdi Goudarzi ◽

...

Keyword(s):

Klebsiella Pneumoniae ◽

Resistance Genes ◽

Hospitalized Patients ◽

Quinolone Resistance

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High frequency of multidrug-resistant (MDR) Klebsiella pneumoniae harboring several β-lactamase and integron genes collected from several hospitals in the north of Iran

Annals of Clinical Microbiology and Antimicrobials ◽

10.1186/s12941-021-00476-1 ◽

2021 ◽

Vol 20 (1) ◽

Author(s):

Mojgan Farhadi ◽

Mohammad Ahanjan ◽

Hamid Reza Goli ◽

Mohammad Reza Haghshenas ◽

Mehrdad Gholami

Keyword(s):

Klebsiella Pneumoniae ◽

Resistance Genes ◽

Antimicrobial Susceptibility ◽

Hospitalized Patients ◽

Clinical Samples ◽

High Morbidity ◽

Drug Resistant ◽

The North ◽

Agar Diffusion Test ◽

North Of Iran

Abstract Background Klebsiella pneumoniae is one of the leading causes of hospital outbreaks worldwide. Also, antibiotic-resistant K. pneumoniae is progressively being involved in invasive infections with high morbidity and mortality. The aim of the current study was to determine antimicrobial susceptibility patterns and the incidence of resistance genes (integron types and β-lactamase-encoded genes) among clinical isolates of K. pneumoniae. Methods In this cross-sectional study, a total of 100 clinical samples were obtained from hospitalized patients in three teaching hospitals in the north of Iran, from November 2018 and October 2019. Antimicrobial susceptibility testing was performed using disk agar diffusion test in line with CLSI recommendations. For colistin, minimum inhibitory concentration (MIC) was determined using broth microdilution. Based on antibiogram, multi-drug resistant (MDR) and extensive-drug resistant (XDR) strains were detected. Finally, integron types and β-lactamase resistance genes were identified using polymerase chain reaction technique. Results The most and least clinical samples were related to the urine and bronchoalveolar lavage, respectively. Based on the antibiogram results, amikacin and gentamicin exhibited good activity against K. pneumoniae strains in vitro. The high resistance rate (93%) to ampicillin/sulbactam predicts the limited efficacy of this antibiotic, in the hospitals studied. Among all the 100 isolates, the frequency of MDR and XDR phenotypes were 58% and 13%, respectively, while no pan-drug resistant (PDR) strains were found. In the MDR K. pneumoniae strains, the prevalence of blaSHV, blaTEM, blaCTX-M-15, blaKPC, blaOXA-48, blaNDM β-lactamase genes were 91.4%, 82.7%, 79.3%, 29.3%, 36.2% and 6.9%, respectively, however 91.4% of the isolates were carrying intI gene. Class II and III integrons were not detected in any isolates. Conclusion The MDR K. pneumoniae is becoming a serious problem in hospitals, with many strains developing resistance to most available antimicrobials. Our results indicate co-presence of a series of β-lactamase and integron types on the MDR strains recovered from hospitalized patients. The increasing rate of these isolates emphasizes the importance of choosing an appropriate antimicrobial regimen based on antibiotic susceptibility pattern.

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PREVALENCE OF EXTENDED SPECTRUM BETA-LACTAMASES (ESBLS) PRODUCING ESCHERICHIA COLI AND KLEBSIELLA PNEUMONIAE AMONG HOSPITALIZED PATIENTS FROM NIGERIA

FUDMA Journal of Sciences ◽

10.33003/fjs-2021-0502-676 ◽

2021 ◽

Vol 5 (2) ◽

pp. 584-595

Author(s):

Olivia Sochi Egbule ◽

Bernard O. Ejechi

Keyword(s):

Escherichia Coli ◽

Klebsiella Pneumoniae ◽

General Hospital ◽

Hospitalized Patients ◽

Diffusion Method ◽

Alkaline Lysis ◽

Beta Lactamases ◽

Resistance Patterns ◽

Extended Spectrum Beta Lactamases ◽

Synergy Test

The aim of this study was to determine the resistance patterns and ESBLs production among clinical isolates of Escherichia coli and Klebsiella pneumoniae in two government hospitals of Delta State, Nigeria. Urine, blood and wound samples were aseptically collected from hospitalized patients, bacteriologically processed and isolates identified using standard protocols. Antimicrobial susceptibility testing was determined by disc diffusion method. The plasmid DNA of Multidrug resistance (MDR) isolates were extracted by alkaline lysis method. Phenotypic ESBL production of the MDR isolates was done by Double Disc Synergy Test (DDST) while PCR was used to detect blaCTX-M, blaSHV and blaTEM among isolates. A total of 217 isolates were obtained, of which 161(74.2%) and 56(25.8%) were Escherichia coli and Klebsiella pneumoniae respectively. The antimicrobial resistance varied from one location to another. All isolates obtained from blood of general hospital Warri (GHW) were 100% resistant to amoxicillin clavulanic acid and the cephalosporins (ceftazidime, cefotaxime, and cefuroxime). Isolates from General hospital Agbor (GHA) showed high resistance of 75.0% to cefotaxime, 93.8% to each of ceftazidime and cefuroxime. Overall low resistance to nitrofurantoin was observed in E. coli isolates obtained from urine of GHW (27.5%) and GHA (20.8%). Out of 217 isolates, 75.1% (163/217) were MDR, of which 36.8% and 39.3% produced ESBL by DDST and PCR respectively. The most common ESBL gene was blaCTX-M expressed by 28(17.2%) of the isolates. The high prevalence of MDR and ESBL underscores the need for a continuous local monitoring of antibiotic resistance.

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