scholarly journals Guidance for Choosing When to Use Electron and/or Light Microscopy and Related ASTM E4 Standards

2004 ◽  
Vol 12 (2) ◽  
pp. 26-27 ◽  
Author(s):  
R. C. Nester
Keyword(s):  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Light Microscopy ◽  
Imaging Systems ◽  
Light Micrograph ◽  
Investigative Techniques ◽  
Scanning Electron

Technical publications sometimes include scanning electron images to characterize a microstructure, when the relevant structure could have very easily been illustrated using a simple light micrograph. When should one use a light generated image? What are the advantages/disadvantages of an electron generated image, particularly; one generated using a scanning electron microscope (SEM)? While there is some overlap in the capabilities of these imaging systems; in general, they are complementary tools, each with their own uses. Standards under the jurisdiction of ASTM Committee E4 on Metallography offer guidance to both new and experienced users of both investigative techniques.

The Broad Applications of the Scanning Electron Microscope

1969 ◽  
Vol 27 ◽  
pp. 20-21
Author(s):  
C. T. Nightingale ◽  
S. E. Summers ◽  
T. P. Turnbull
Keyword(s):  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Light Microscopy ◽  
Surface Topography ◽  
Great Depth ◽  
Resolving Power ◽  
Depth Of Field ◽  
Pictorial Representations ◽  
Scanning Electron

The ease of operation of the scanning electron microscope has insured its wide application in medicine and industry. The micrographs are pictorial representations of surface topography obtained directly from the specimen. The need to replicate is eliminated. The great depth of field and the high resolving power provide far more information than light microscopy.

Additions to the technique of epi-illumination light microscopy for the study of floral and vegetative apices

1989 ◽  
Vol 67 (6) ◽  
pp. 1739-1743 ◽  
Author(s):  
W. A. Charlton ◽  
A. D. Macdonald ◽  
U. Posluszny ◽  
C. P. Wilkins
Keyword(s):  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Light Microscopy ◽  
Staining Techniques ◽  
Scanning Electron

Various staining techniques for apices destined for epi-illumination light microscopy are described and discussed. Appropriate modifications to photographic procedures are described. A technique is described for the subsequent observation of specimens in a scanning electron microscope fitted with a cryo-stage.

scholarly journals Toward Operations in a Surgical Scenario: Characterization of a Microgripper via Light Microscopy Approach

2019 ◽  
Vol 9 (9) ◽  
pp. 1901 ◽  
Author(s):  
Federica Vurchio ◽  
Pietro Ursi ◽  
Francesco Orsini ◽  
Andrea Scorza ◽  
Rocco Crescenzi ◽  
...  
Keyword(s):  
Electron Microscope ◽  
Statistical Analysis ◽  
Scanning Electron Microscope ◽  
Light Microscopy ◽  
Mechanical Systems ◽  
Optical Microscope ◽  
Micro Electro Mechanical Systems ◽  
Mems Technology ◽  
Scanning Electron

Micro Electro Mechanical Systems (MEMS)-Technology based micro mechanisms usually operate within a protected or encapsulated space and, before that, they are fabricated and analyzed within one Scanning Electron Microscope (SEM) vacuum specimen chamber. However, a surgical scenario is much more aggressive and requires several higher abilities in the microsystem, such as the capability of operating within a liquid or wet environment, accuracy, reliability and sophisticated packaging. Unfortunately, testing and characterizing MEMS experimentally without fundamental support of a SEM is rather challenging. This paper shows that in spite of large difficulties due to well-known physical limits, the optical microscope is still able to play an important role in MEMS characterization at room conditions. This outcome is supported by the statistical analysis of two series of measurements, obtained by a light trinocular microscope and a profilometer, respectively.

Comparison of Larval Stored Product Beetle Mandibles by Light Microscope and Scanning Electron Microscope

1981 ◽  
Vol 64 (1) ◽  
pp. 199-224
Author(s):  
John E Kvenberg
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Light Microscopy ◽  
Light Microscope ◽  
Morphological Characteristics ◽  
Scanning Electron ◽  
Conventional Light Microscopy

Abstract Larval stored product beetle mandibles were studied by comparing images made by scanning electron microscopy with those made by conventional light microscopy. Discussion of morphological characteristics is based on illustrations of 25 species

Morphology of the perineal pattern of the root-knot nematodes Meloidogyne hapla and M. incognita

1975 ◽  
Vol 53 (4) ◽  
pp. 370-373 ◽  
Author(s):  
R. H. Mulvey ◽  
P. W. Johnson ◽  
J. L. Townshend ◽  
J. W. Potter
Keyword(s):  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Light Microscopy ◽  
Meloidogyne Hapla ◽  
Root Knot Nematodes ◽  
Scanning Electron

Scanning electron microscope studies of Meloidogyne hapla and M. incognita revealed features of the female perineal pattern previously obscure under light microscopy. M. hapla is characterized by a deep invagination of the cuticle between the anal and vulval areas and a strong demarcation of dorsal and ventral striae. M. incognita lacks the infolding of the cuticle and is characterized by forked striae.

Scanning electron microscopy of sporangia of Coelomomyces

1973 ◽  
Vol 51 (7) ◽  
pp. 1325-1330 ◽  
Author(s):  
C. E. Bland ◽  
J. N. Couch
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Light Microscopy ◽  
Undescribed Species ◽  
Scanning Electron

The scanning electron microscope is used in an examination of the sporangia of 18 described and 3 undescribed species of Coelomomyces. As a possible aid to taxonomy, those species considered in this study are grouped into eight morphologically recognizable groups. The complementary use of light microscopy and scanning electron microscopy in studies of this type is emphasized.

Scanning Electron Microscopy of the Denticles and Eggs of Ascaris lumbricoides and Ascaris suum

1972 ◽  
Vol 30 ◽  
pp. 392-393
Author(s):  
John E. Ubelaker ◽  
Venita F. Allison
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Light Microscopy ◽  
Ascaris Suum ◽  
Slaughter House ◽  
Molar Teeth ◽  
Morphological Differences ◽  
Scanning Electron

Morphological differences between Ascaris of human and porcine origins have been difficult to detect. Sprent has distinguished the two species by the characteristics of the denticles observed by light microscopy. The present study is concerned with the differences in morphology of the denticles and with the examination of the ova of the two species as revealed by scanning electron microscopy.Hospital and slaughter house specimens were acquired locally, fixed in phosphate buffered paraformaldehyde and stored in 70% ethanol overnight. Preparative procedures for scanning electron microscopy were as previously reported. Specimens were examined in a JEOL JSMU-3 scanning electron microscope.The denticles of Ascaris suum are regular in distribution and conoid in shape (Fig. 1, A and B). Each denticle is cuspid; no multiple cusps were observed. The “molar teeth” forms previously described probably result from observations of cusps from which adhering debris was not removed in preparation.

A Simple Light-Microscope Technique for the Examination of Cell Surfaces

1973 ◽  
Vol 13 (1) ◽  
pp. 317-319
Author(s):  
A. ROSS ◽  
I. P. GORMLEY
Keyword(s):  
Heavy Metal ◽  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Light Microscopy ◽  
Light Microscope ◽  
Simple Technique ◽  
Cell Surfaces ◽  
Microscope Technique ◽  
Scanning Electron

A simple technique for the examination of cell surfaces by shadowing them with a heavy metal and examining by light microscopy has been described. Stained preparations can be examined and photographed initially prior to investigation by this technique. It is suggested that this technique could be used instead of the scanning electron microscope or replica techniques where the limited resolution of the light microscope is sufficient.

Spore morphology in the Nidulariaceae (fungi) as revealed by the scanning electron microscope

1977 ◽  
Vol 55 (24) ◽  
pp. 3042-3045 ◽  
Author(s):  
Harold J. Brodie ◽  
H. F. Dietrich
Keyword(s):  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Light Microscopy ◽  
Smooth Surfaces ◽  
Spore Morphology ◽  
Scanning Electron

Basidiospores of species representing each of the five genera in the fungus family Nidulariaceae when examined and photographed using the scanning electron microscope appear to have smooth surfaces. Among the species chosen for study, those representing Mycocalia, Nidularia, and Nidula all possess spores which bear a hilum, which has apparently been overlooked in examinations based on light microscopy.

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