Synthetic spectroscopic models related to coenzymes and base pairs. XI. Solid state ultraviolet irradiation of 1,1'-trimethylenebisthymine and photosensitized irradiation of 1,1'-polymethylenebisthymines

1973 ◽  
Vol 95 (7) ◽  
pp. 2320-2324 ◽  
Author(s):  
Nelson J. Leonard ◽  
Robert S. McCredie ◽  
Marshall W. Logue ◽  
Robert L. Cundall
Keyword(s):  
Solid State ◽  
Ultraviolet Irradiation ◽  
Base Pairs

Proton-detected solid-state NMR detects the inter-nucleotide correlations and architecture of dimeric RNA in microcrystals

2017 ◽  
Vol 53 (96) ◽  
pp. 12886-12889 ◽  
Author(s):  
Yufei Yang ◽  
ShengQi Xiang ◽  
Xiaodan Liu ◽  
Xiaojing Pei ◽  
Pengzhi Wu ◽  
...  
Keyword(s):  
Solid State ◽  
Solid State Nmr ◽  
Base Pairs

A novel proton-detected solid-state NMR experiment detects the NH⋯N H-bonds within the Watson–Crick base pairs of RNA in crystals.

Supramolecular architectures in two 1:1 cocrystals of 5-fluorouracil with 5-bromothiophene-2-carboxylic acid and thiophene-2-carboxylic acid

2017 ◽  
Vol 73 (6) ◽  
pp. 481-485 ◽  
Author(s):  
Marimuthu Mohana ◽  
Packianathan Thomas Muthiah ◽  
Colin D. McMillen
Keyword(s):  
Solid State ◽  
Carboxylic Acid ◽  
Hydrogen Bonds ◽  
Crystal Structures ◽  
X Ray Diffraction ◽  
Base Pairs ◽  
X Ray ◽  
Supramolecular Architectures ◽  
Acid Acid ◽  
Carboxylic Acid Dimer

In solid-state engineering, cocrystallization is a strategy actively pursued for pharmaceuticals. Two 1:1 cocrystals of 5-fluorouracil (5FU; systematic name: 5-fluoro-1,3-dihydropyrimidine-2,4-dione), namely 5-fluorouracil–5-bromothiophene-2-carboxylic acid (1/1), C5H3BrO2S·C4H3FN2O2, (I), and 5-fluorouracil–thiophene-2-carboxylic acid (1/1), C4H3FN2O2·C5H4O2S, (II), have been synthesized and characterized by single-crystal X-ray diffraction studies. In both cocrystals, carboxylic acid molecules are linked through an acid–acid R 2 2(8) homosynthon (O—H...O) to form a carboxylic acid dimer and 5FU molecules are connected through two types of base pairs [homosynthon, R 2 2(8) motif] via a pair of N—H...O hydrogen bonds. The crystal structures are further stabilized by C—H...O interactions in (II) and C—Br...O interactions in (I). In both crystal structures, π–π stacking and C—F...π interactions are also observed.

Covalent Organic Polymers with Solid-State Dual-Color Fluorescence Tunable by Ultraviolet Irradiation

2022 ◽  
Author(s):  
Yan Chen ◽  
Wei Li ◽  
Rong-Zhi Gao ◽  
Xiao-Han Wang ◽  
An-na Tang ◽  
...  
Keyword(s):  
Solid State ◽  
Strong Solution ◽  
Ultraviolet Irradiation ◽  
Aldol Condensation ◽  
Fluorescence Property ◽  
Organic Polymers ◽  
Dual Fluorescence ◽  
Dual Color

An aldol condensation-based strategy is developed for preparing covalent organic polymers (COPs) with strong solution and solid-state dual fluorescence. Moreover, the fluorescence property can be facilely tuned by two ways:...

Improved solid-state stability of salts by cocrystallization between conjugate acid–base pairs

CrystEngComm ◽  
2013 ◽  
Vol 15 (29) ◽  
pp. 5756 ◽  
Author(s):  
Sathyanarayana Reddy Perumalla ◽  
Changquan Calvin Sun
Keyword(s):  
Solid State ◽  
Acid Base ◽  
Base Pairs ◽  
Conjugate Acid ◽  
Solid State Stability

transoid and cisoid Conformations in Silver-Mediated Cytosine Base Pairs: Hydrogen Bonding Dictates Argentophilic Interactions in the Solid State

2019 ◽  
Vol 58 (19) ◽  
pp. 13346-13352 ◽  
Author(s):  
Liam Mistry ◽  
Paul G. Waddell ◽  
Nick G. Wright ◽  
Benjamin R. Horrocks ◽  
Andrew Houlton
Keyword(s):  
Hydrogen Bonding ◽  
Solid State ◽  
Base Pairs ◽  
Argentophilic Interactions

scholarly journals Identification of RNA base pairs and complete assignment of nucleobase resonances by proton-detected solid-state NMR spectroscopy at 100 kHz MAS

2021 ◽  
Author(s):  
Philipp Innig Aguion ◽  
John Kirkpatrick ◽  
Teresa Carlomagno ◽  
Alexander Marchanka
Keyword(s):  
Solid State ◽  
Solid State Nmr ◽  
Rna Structure ◽  
Rna Secondary Structure ◽  
Spin Systems ◽  
Base Pairs ◽  
Cellular Processes ◽  
Complete Assignment ◽  
Low Sensitivity ◽  
Rna Complexes

Knowledge of RNA structure, either in isolation or in complex, is fundamental to understand the mechanism of cellular processes. Solid-state NMR (ssNMR) is applicable to high molecular-weight complexes and does not require crystallization; thus, it is well-suited to study RNA as part of large multicomponent assemblies. Recently, we solved the first structures of both RNA and an RNA-protein complex by ssNMR using conventional 13C- and 15N-detection. This approach is limited by the severe overlap of the RNA peaks together with the low sensitivity of multidimensional experiments. Here, we overcome the limitations in sensitivity and resolution by using 1H-detection at fast MAS rates. We develop experiments that allow the identification of complete nucleobase spin-systems together with their site-specific base pair pattern using sub-milligram quantities of one uniformly labelled RNA sample. These experiments provide rapid access to RNA secondary structure by ssNMR in protein-RNA complexes of any size.

scholarly journals Identification of weak non-canonical base pairs around riboswitch-ligand recognition sites by solid-state NMR exchange spectroscopy

2021 ◽  
Author(s):  
Sha Zhao ◽  
Ziyang Wen ◽  
Xinming Li ◽  
Mengbing zou ◽  
Ge Yu ◽  
...  
Keyword(s):  
Solid State ◽  
Base Pair ◽  
Solid State Nmr ◽  
Chemical Exchange ◽  
Critical Role ◽  
Building Blocks ◽  
Weak Base ◽  
Base Pairs ◽  
Canonical Base ◽  
Exchange Spectroscopy

Abstract Base pairs are fundamental building blocks of RNA structures, and their stability and open-close equilibrium constitutes the dynamic picture. Weak base pairs, which feature the characteristics of low stability and rapid base pair opening, often play a critical role in RNA functions. However, site-specific identification of weak base pairs in RNA is challenging. Here, we report a solid-state NMR (SSNMR)-based two-dimensional proton-detected water–RNA exchange spectroscopy (WaterREXSY) to address this challenge. The approach uses the chemical exchange between hydrogen-bonded imino protons within the base pair and excited water molecules to polarize the imino protons for SSNMR observation. This process takes advantages that the imino protons within weak pairs undergo fast exchange rates with water, enabling a quick build-up and efficient detection. This method is used to characterize the weak pair in the riboA71–adenine complex (i.e., the 71nt-aptamer domain of the add adenine riboswitch from Vibrio vulnificus). We identify U47•U51, a weak non-canonical base pair that constitutes the U47•U51•(adenine-U74) base tetrad around the ligand-binding pocket. This result suggests that the breakage of U47•U51 may be the early stage in the process of ligand release.

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