scholarly journals Amiodarone inhibits arrhythmias in hypertensive rats by improving myocardial biomechanical properties

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Yifeng Nie ◽  
Yin He ◽  
Dong Han ◽  
Yuansheng Liu ◽  
Xiang Li
Keyword(s):  
Spontaneously Hypertensive Rats ◽  
High Throughput Sequencing ◽  
Ventricular Myocytes ◽  
Mechanical Stretch ◽  
Biomechanical Properties ◽  
Hypertensive Rats ◽  
Myocardial Mechanics ◽  
Spontaneously Hypertensive ◽  
Single Ventricular Myocytes ◽  
Biomechanical Stimulation

AbstractThe prevalence of arrhythmia in patients with hypertension has gradually attracted widespread attention. However, the relationship between hypertension and arrhythmia still lacks more attention. Herein, we explore the biomechanical mechanism of arrhythmia in hypertensive rats and the effect of amiodarone on biomechanical properties. We applied micro-mechanics and amiodarone to stimulate single ventricular myocytes to compare changes of mechanical parameters and the mechanism was investigated in biomechanics. Then we verified the expression changes of genes and long non-coding RNAs (lncRNAs) related to myocardial mechanics to explore the effect of amiodarone on biomechanical properties. The results found that the stiffness of ventricular myocytes and calcium ion levels in hypertensive rats were significantly increased and amiodarone could alleviate the intracellular calcium response and biomechanical stimulation. In addition, experiments showed spontaneously hypertensive rats were more likely to induce arrhythmia and preoperative amiodarone intervention significantly reduced the occurrence of arrhythmias. Meanwhile, high-throughput sequencing showed the genes and lncRNAs related to myocardial mechanics changed significantly in the spontaneously hypertensive rats that amiodarone was injected. These results strengthen the evidence that hypertension rats are prone to arrhythmia with abnormal myocardial biomechanical properties. Amiodarone effectively inhibit arrhythmia by improving the myocardial biomechanical properties and weakening the sensitivity of mechanical stretch stimulation.

Effects of Amlodipine on Bone Metabolism in Orchidectomised Spontaneously Hypertensive Rats

Pharmacology ◽  
2018 ◽  
Vol 102 (1-2) ◽  
pp. 67-73
Author(s):  
Helena Zivna ◽  
Iveta Gradošová ◽  
Pavel Zivny ◽  
Eva Cermakova ◽  
Vladimir Palicka
Keyword(s):  
Bone Metabolism ◽  
Spontaneously Hypertensive Rats ◽  
Negative Impact ◽  
Biomechanical Testing ◽  
Biomechanical Properties ◽  
Hypertensive Rats ◽  
Mineral Density ◽  
Spontaneously Hypertensive ◽  
Osteopenic Bone

Spontaneously hypertensive rats (SHR) represent a model of essential hypertension. We studied the effect of amlodipine (AML) on bone markers, bone mineral density (BMD), and biomechanical properties of osteopenic bone induced by orchidectomy in male SHR. Rats were allocated to 3 groups and were sacrificed after 12 weeks: sham-operated control; orchidectomised control; and orchidectomised receiving a diet supplemented with AML. Indicators of bone turnover were assessed in bone homogenate, BMD was measured by dual energy X-ray absorptiometry, and the femurs were subjected to biomechanical testing. Long-term AML administration does not have a negative impact on bone metabolism and density in male SHR.

scholarly journals Ameliorated biomechanical properties of carotid arteries by puerarin in spontaneously hypertensive rats

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Xiaoxia Fang ◽  
Sheng Dong ◽  
Yun Wu ◽  
Yun He ◽  
Min Lu ◽  
...  
Keyword(s):  
Vascular Remodeling ◽  
Spontaneously Hypertensive Rats ◽  
Carotid Arteries ◽  
Transient Receptor Potential ◽  
Mechanical Test ◽  
Ischemia Reperfusion ◽  
Biomechanical Properties ◽  
Transient Receptor Potential Channels ◽  
Hypertensive Rats ◽  
Spontaneously Hypertensive

Abstract Background An emerging body of evidence indicates that puerarin (PUE) plays an important role in the treatment of angina pectoris, myocardial ischemia-reperfusion injury, hypertension and other cardiovascular diseases, but how PUE affects the vascular remodeling of hypertensive rats has not been reported yet. This study aimed to investigate the effect and mechanism of PUE on carotid arteries of spontaneously hypertensive rats (SHR) to provide the basis for the clinical application of PUE. Methods Thirty male SHR and six male Wistar Kyoto rats (WKY) aged 3 months were used in this study, SHR rats were randomly divided into 5 groups, PUE(40 or 80 mg/kg/d, ip) and telmisartan (TELMI) (30 mg/kg/d, ig) were administrated for 3 months. We use DMT myography pressure-diameter system to investigate biomechanical properties of carotid arteries, 10 μM pan-classical transient receptor potential channels (TRPCs) inhibitor SKF96365, 200 nM specific TRPC6 inhibitor SAR7334 and 100 μM Orai1 inhibitor ANCOA4 were used in the mechanical test. Results PUE can significantly decrease systolic and diastolic blood pressure, long-term administration of PUE resulted in a mild reduction of thickness and inner diameter of carotid artery. PUE ameliorate NE-response and vascular remodeling mainly through inhibiting TRPCs channel activities of VSMC. Conclusion PUE can ameliorate biomechanical remodeling of carotid arteries through inhibiting TRPCs channel activities of VSMC in spontaneously hypertensive rats.

Contractile properties of left ventricular myocytes isolated from spontaneously hypertensive rats

1995 ◽  
Vol 13 (12) ◽  
pp. 1803???1807 ◽  
Author(s):  
Takamasa Kobayashi ◽  
Mareomi Hamada ◽  
Hideki Okayama ◽  
Yuji Shigematsu ◽  
Takumi Sumimoto ◽  
...  
Keyword(s):  
Spontaneously Hypertensive Rats ◽  
Ventricular Myocytes ◽  
Left Ventricular ◽  
Contractile Properties ◽  
Hypertensive Rats ◽  
Spontaneously Hypertensive
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