Phytochemical analysis of Clerodendron trichotomum by UHPLC-ESI-MS

In the search for bioactive constituents from Vietnamese plants, the leaves and stems of Callistemon citrinus (Curtis) Skeels were selected for chemical investigation. Phytochemical analysis of plant led to the isolation of eight phenolic compounds including two flavonoids (eucalyptine (1) and 8-demethyleucalyptine (2)), two alcohols (blumenol A (3), n-tetratriacontanol (4)), three benzoic acid derivatives (acid gallic (5), methyl gallate (6) protocatechuic acid (7)), one sterol (b-sitosterol (8)), and along with one sesquiterpene (2,6,10-bisabolatriene (9)). The structures of the natural compounds were determined by spectroscopic evidences including 1D- and 2D-NMR and ESI-MS.

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New Rocaglamide Derivatives from Vietnamese Aglaia species

Natural Product Communications ◽

10.1177/1934578x1400900627 ◽

2014 ◽

Vol 9 (6) ◽

pp. 1934578X1400900

Author(s):

Ngoc Tu Duong ◽

RuAngelie Edrada-Ebel ◽

Rainer Ebel ◽

Wenhan Lin ◽

Anh Tuan Duong ◽

...

Keyword(s):

Nmr Spectroscopy ◽

Insecticidal Activity ◽

Spodoptera Littoralis ◽

2D Nmr ◽

Mass Spectrometric ◽

Phytochemical Analysis ◽

2D Nmr Spectroscopy ◽

Esi Ms

Phytochemical analysis of the leaves of different Aglaia species collected in Vietnam yielded eight rocaglamide derivatives, which are responsible for the strong insecticidal activity against Spodoptera littoralis, including rocaglamide A (1), rocaglamide I (2), rocaglamide W (3), rocaglamide AB (4), rocaglamide J (5), rocaglaol (6), rocaglamide S (7) and the new rocaglamide AY (8). The structures of these compounds were elucidated through extensive 1D and 2D NMR spectroscopy and analysis of their mass spectrometric (ESI-MS) and HRESIMS data.

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Phytochemical analysis of an antiviral fraction of Radix astragali using HPLC–DAD–ESI–MS/MS

Journal of Natural Medicines ◽

10.1007/s11418-009-0381-1 ◽

2009 ◽

Vol 64 (2) ◽

pp. 182-186 ◽

Cited By ~ 9

Author(s):

Li Tang ◽

Ying Liu ◽

Yeling Wang ◽

Chunlin Long

Keyword(s):

Phytochemical Analysis ◽

Radix Astragali ◽

Esi Ms

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RP-HPLC-UV-ESI-MS phytochemical analysis of fruits of Conocarpus erectus L.

Chemical Papers ◽

10.2478/s11696-014-0570-6 ◽

2014 ◽

Vol 68 (10) ◽

Cited By ~ 4

Author(s):

El-Sayed Abdel-Hameed ◽

Salih Bazaid ◽

Mohamed Shohayeb

Keyword(s):

Antioxidant Properties ◽

Reversed Phase ◽

In Vivo Studies ◽

Phytochemical Analysis ◽

Meoh Extract ◽

Esi Ms ◽

Conocarpus Erectus ◽

Rp Hplc

AbstractPrevious work revealed that the defatted methanol (MeOH) extract of fruits of Conocarpus erectus L. (Combretaceae family) exhibited antioxidant, antibacterial and anti-cancer activities. In further studies of this valuable plant, the defatted MeOH extract of C. erectus fruits was subjected to chromatographic fractionation in a silica gel glass column followed by reversed-phase high-performance liquid chromatography-ultraviolet-electrospray ionisation spectrometry analysis (RP-HPLC-UV-ESI-MS). The major and sharp peaks in each sample were identified or tentatively identified based on matching with some standard compounds and a review of the literature. Ellagic acid, vescalagin/castalagin isomer and di-(hexahydroxy diphenoyl) galloyl hexose isomer were tentatively identified as major components with many hydrolysable types of tannins on the basis of a comparison of its mass patterns with relevant items in the literature. Gallic acid, kaempferol 3-O-β-d-glucopyranoside and quercetin 3-O-β-d-glucopyranoside were identified on the basis of matching retention time (t R) and mass spectra with the standards. Polymethoxylated flavonoid isomers were also tentatively identified. The antioxidant properties of all samples were found to be associated with the total content of phenolic compounds. This may be considered as the first detailed phytochemical report in identifying the phytochemicals in C. erectus fruits. Due to the high antioxidant activity exhibited by both the crude MeOH extract and its fractions, it could be used as an effective natural antioxidant after further in vitro and in vivo studies.

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Phytochemical analysis of trifoliate orange during fermentation by HPLC–DAD–ESI–MS/MS coupled with multivariate statistical analysis

Acta Chromatographica ◽

10.1556/1326.2020.00818 ◽

2020 ◽

Author(s):

Dan Gao ◽

Chong Woon Cho ◽

Le Ba Vinh ◽

Jin Hyeok Kim ◽

Young Ho Kim ◽

...

Keyword(s):

Quality Control ◽

Statistical Analysis ◽

Multivariate Statistical Analysis ◽

Partial Least Square ◽

Array Detector ◽

Phytochemical Analysis ◽

Chemical Compositions ◽

Trifoliate Orange ◽

Multivariate Statistical ◽

Esi Ms

AbstractDuring the process of fermentation, the chemical compositions of trifoliate orange (Poncirus trifoliate (L). Raf) changed greatly. To provide a completely phytochemical profile, high-performance liquid chromatography-diode array detector-hyphenated with tandem mass spectrometry (HPLC–DAD–ESI-MS/MS) has been successfully applied to screen and identify the unknown constituents of trifoliate orange during fermentation, which make it available for the quality control of fermented products. Multivariate statistical analysis was performed to classify the trifoliate oranges based on the status of fermentation. A total of 8 components were identified among the samples. Hierarchical Clustering Analysis (HCA) and Principal Component Analysis (PCA) demonstrated the fermented and unfermented trifoliate oranges were obviously different, an effective and reliable Partial Least Square Discriminate Analysis (PLS-DA) technique was more suitable to provide accurate discrimination of test samples based their different chemical patterns. Furthermore, a permutation validated the reliability of PLS-DA and variable importance plot revealed that the characterized syringing, naringin, and poncirin showed the high ability to distinguish the trifoliate oranges during fermentation. The present investigation could provide detailed information for the quality control and evaluation of trifoliate oranges during the fermentation process.

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