Maternal Epilepsy and Umbilical Cord Blood Oxidative Stress Level

Objective. Using a novel assay termed prooxidant-antioxidant balance (PAB) assay to determine prooxidant-antioxidant balance in umbilical cord blood of infants with meconium stained of amniotic fluid (MSAF). Passage of meconium in amniotic fluid is associated with increase of neonatal mortality and morbidity. This complication occurs in about 15% of infants and is more widespread in postterm neonates. About 1.5 percent of neonates with MSAF develop meconium aspiration syndrome.Method. Sera of 29 umbilical cord blood of infants with MSAF and 32 healthy infants (HI) were collected. Both groups had nonsmoker and non-alcoholic mothers with no diseases. The PAB was measured.Result. There was a significant increase of PAB value (32.8±15.9 HK) in umbilical cord blood of infants with MSAF in comparison to HI (24.5±12.6 HK) (P<0.05). There was no significant correlation between PAB value and age of mothers.Conclusion. The increased PAB value in infants with MSAF showed that these infants are exposed to oxidative stress. Further research with larger population is needed to demonstrate the oxidative stress in infants with MSAF.

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Comparison of oxidative stress markers in umbilical cord blood after vaginal and cesarean delivery

Obstetrics & Gynecology Science ◽

10.5468/ogs.2014.57.2.109 ◽

2014 ◽

Vol 57 (2) ◽

pp. 109 ◽

Cited By ~ 19

Author(s):

Eun Ji Noh ◽

Yoon Ha Kim ◽

Moon Kyoung Cho ◽

Jong Woon Kim ◽

Jin Wook Kim ◽

...

Keyword(s):

Oxidative Stress ◽

Cord Blood ◽

Cesarean Delivery ◽

Umbilical Cord ◽

Umbilical Cord Blood ◽

Oxidative Stress Markers ◽

Stress Markers

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Transplacental Transmission of Filarial Infections and Its Impact on Oxidative Stress and Immune Responses during Foetal Life in Bamenda, North West Cameroon

International Journal of TROPICAL DISEASE & Health ◽

10.9734/ijtdh/2020/v41i1330345 ◽

2020 ◽

pp. 26-38

Author(s):

Lemnui Benardette ◽

Oumar Mahamat ◽

Ntonifor Helen Ngum ◽

Ngwenyi Annie- Dorette ◽

Theresia Kuo Akenji

Keyword(s):

Oxidative Stress ◽

Immune Response ◽

Cord Blood ◽

Umbilical Cord ◽

Umbilical Cord Blood ◽

Colorimetric Method ◽

Transplacental Transmission ◽

Oxidative Stress Status ◽

Total Oxidative Stress ◽

Umbilical Cords

Background: Women commonly harbour filarial infections during their childbearing years, raising the possibility that the developing foetus may be exposed to filarial antigens in the uterus and thereby have altered immunity and susceptibility to infection during early childhood. However, there are no concrete proofs to justify the risk of infections in infants born from mothers having filarial infections during pregnancy. Aim: The purpose of this study was to assess the prevalence of microfilariae in umbilical cord blood and respective mothers and to evaluate the relationship between the cord blood filarial infection and the oxidative stress status and concentration of IL-2, IL-10, IL-13, INF-γ and IgG in umbilical cord blood. Methods: This was a nested case–control study of cords and mothers of normal gestational age (>250 days of gestation). A total of 316 pairs of umbilical cords and mothers were examined. The presence of microfilariae was assessed by microscopy in mothers and cords. Oxidative stress status (total oxidative stress and total oxidative defence) and nitric oxide of umbilical cord and mother’s blood were investigated by the colorimetric method. ELISA was carried out for IL-2, IL-10, IL-13, INF-γ in umbilical cord and mother’s blood. Equally, umbilical cords were subjected to ELISA for total IgG. Results: Results obtained showed that microfilariae had a prevalence of 32.9% and 29.7% in umbilical cord blood and women at time of delivery respectively. High levels of total oxidative stress (TOS) with low total oxidative defence (TAD) was found in filarial infected (Mf +ve) umbilical cord and mother’s blood compared to controls or uninfected (Mf -ve) cords and mothers blood. IL-2 was lower in the blood of microfilariae infected cords and mothers, while INF-γ, IL-13 and IL-10 were higher as compared to microfilariae negative cords and mothers. Equally, plasma total IgG concentration was higher in microfilariae positive cords compared to the negative cords and positively correlated with IL-10. Conclusions: There is high frequency of transplacental transmission of microfilariae. Cord blood filarial infections were associated with a high TOS, a protective immune response with low IL-2 and high INF-γ, and a typical Th2 immune response that was associated with higher concentration of immune total IgG regulatory cytokine IL-10 and IL-13 in neonate.

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Oxidative stress status in umbilical cord blood from neonates born to mothers with atopic asthma

The Journal of Maternal-Fetal & Neonatal Medicine ◽

10.3109/14767058.2013.811228 ◽

2013 ◽

Vol 27 (2) ◽

pp. 192-196 ◽

Cited By ~ 3

Author(s):

Yang Ling ◽

Guo Yin-Shi ◽

Shan Hong-Wei ◽

Zhou Yan ◽

Li Qiang ◽

...

Keyword(s):

Oxidative Stress ◽

Cord Blood ◽

Umbilical Cord ◽

Umbilical Cord Blood ◽

Atopic Asthma ◽

Oxidative Stress Status

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Differential expression of markers of oxidative stress and apoptosis in relation to serum ferritin levels in patients with pre-eclampsia

BioMedica ◽

10.51441/biomedica/5-512 ◽

2021 ◽

Vol 37 (3) ◽

pp. 1-8

Author(s):

Jianying Yan ◽

Jie Dong ◽

Xiaoqian Lin ◽

Lichun Chen ◽

Zhuanji Fang ◽

...

Keyword(s):

Oxidative Stress ◽

Cord Blood ◽

Umbilical Cord ◽

Serum Ferritin ◽

Umbilical Cord Blood ◽

Cell Apoptosis ◽

Late Onset ◽

Placental Tissue ◽

Control Group ◽

Pregnant Females

Background and Objective: Pre-eclampsia (PE) is a hypertensive gestational disease appearing during second trimester of pregnancy. Free radicals are released by the placenta in this condition that may cause oxidative damage. This study was designed to determine the serum ferritin (SF) levels in maternal blood, fetal umbilical cord blood and placenta and the changes associated with oxidative stress as well as cell apoptosis to understand the pathogenesis of PE. Methods: This cross-sectional analytical study recruited 60 pregnant females with severe PE and assigned into early and late onset PE groups. Another n = 60 cases of normal pregnant females with similar gestational weeks were selected in the control group. Maternal serum and fetal umbilical cord blood ferritin levels were determined by automatic biochemical immunoassay system. Reverse transcription real-time fluorescence, Western blot and colorimetry were used to determine mRNA expression levels of ferritin and ferritin heavy chain, relative expression of ferritin and superoxide dismutase, malondialdehyde (MDA), and glutathione peroxidase (GSH-Px) levels, respectively. Results: Mean age was 30.89 ± 5.65 and 31.79 ± 5.06 years in early and late onset PE groups respectively. Serum uric acid and creatinine levels of both PE groups were significantly higher than the normal pregnant females. SF levels were also higher in the participants of PE groups. However, the mRNA and ferritin protein levels in placental tissue were significantly lower in PE groups while comparing to controls. The cleaved caspase-3 protein, GSH-Px and MDA levels were significantly higher in both PE groups (p < 0.05). Conclusion: The alterations in factors related to oxidative stress and cell apoptosis in placental tissue may be helpful to understand the pathogenesis of PE and may provide potential biomarkers for the diagnosis of PE.

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