scholarly journals Effect of arginine and threonine in ovo supplementation on immune responses and some serum biochemical attributes in broiler chickens

2018 ◽  
Vol 18 (1) ◽  
pp. 342-349 ◽  
Author(s):  
Majid Toghyani ◽  
Shohreh Tahmasebi ◽  
Mehrdad Modaresi ◽  
Sayed Sadra Ale Saheb Fosoul
Keyword(s):  
Immune Responses ◽  
Broiler Chickens ◽  
In Ovo ◽  
Serum Biochemical

scholarly journals Molecular Response in Intestinal and Immune Tissues to in Ovo Administration of Inulin and the Combination of Inulin and Lactobacillus lactis Subsp. cremoris

2021 ◽  
Vol 7 ◽  
Author(s):  
Aleksandra Dunislawska ◽  
Agnieszka Herosimczyk ◽  
Adam Lepczynski ◽  
Petr Slama ◽  
Anna Slawinska ◽  
...  
Keyword(s):  
Gene Expression ◽  
Energy Metabolism ◽  
Immune Responses ◽  
Intestinal Microbiota ◽  
Broiler Chickens ◽  
In Ovo ◽  
Altered Expression ◽  
Immune Tissues ◽  
Lactobacillus Lactis ◽  
Stimulation Of

Intestinal microbiota are a key factor in maintaining good health and production results in chickens. They play an important role in the stimulation of immune responses, as well as in metabolic processes and nutrient digestion. Bioactive substances such as prebiotics, probiotics, or a combination of the two (synbiotic) can effectively stimulate intestinal microbiota and therefore replace antibiotic growth promoters. Intestinal microbiota might be stimulated at the early stage of embryo development in ovo. The aim of the study was to analyze the expression of genes related to energy metabolism and immune response after the administration of inulin and a synbiotic, in which lactic acid bacteria were combined with inulin in the intestines and immune tissues of chicken broilers. The experiment was performed on male broiler chickens. Eggs were incubated for 21 days in a commercial hatchery. On day 12 of egg incubation, inulin as a prebiotic and inulin with Lactobacillus lactis subsp. cremoris as a synbiotic were delivered to the egg chamber. The control group was injected with physiological saline. On day 35 post-hatching, birds from each group were randomly selected and sacrificed. Tissues (spleen, cecal tonsils, and large intestine) were collected and intended for RNA isolation. The gene panel (ABCG8, HNF4A, ACOX2, APBB1IP, BRSK2, APOA1, and IRS2) was selected based on the microarray dataset and biological functions of genes related to the energy metabolism and immune responses. Isolated RNA was analyzed using the RT-qPCR method, and the relative gene expression was calculated. In our experiment, distinct effects of prebiotics and synbiotics following in ovo delivery were manifested in all analyzed tissues, with the lowest number of genes with altered expression shown in the large intestines of broilers. The results demonstrated that prebiotics or synbiotics provide a potent stimulation of gene expression in the spleen and cecal tonsils of broiler chickens. The overall number of gene expression levels and the magnitude of their changes in the spleen and cecal tonsils were higher in the group of synbiotic chickens compared to the prebiotic group.

scholarly journals In ovo co-administration of vitamins (A and D) and probiotic lactobacilli modulates immune responses in broiler chickens

2022 ◽  
pp. 101717
Author(s):  
Mohammadali Alizadeh ◽  
Jake Astill ◽  
Nadiyah Alqazlan ◽  
Bahram Shojadoost ◽  
Khaled Taha-Abdelaziz ◽  
...  
Keyword(s):  
Immune Responses ◽  
Broiler Chickens ◽  
In Ovo ◽  
Probiotic Lactobacilli

scholarly journals Calcium Carbonate Nanoparticles—Toxicity and Effect of In Ovo Inoculation on Chicken Embryo Development, Broiler Performance and Bone Status

Animals ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 932
Author(s):  
Arkadiusz Matuszewski ◽  
Monika Łukasiewicz ◽  
Jan Niemiec ◽  
Maciej Kamaszewski ◽  
Sławomir Jaworski ◽  
...  
Keyword(s):  
Calcium Carbonate ◽  
Embryo Development ◽  
Bone Quality ◽  
Chicken Embryo ◽  
Broiler Chickens ◽  
Selection Procedure ◽  
High Specificity ◽  
Control Group ◽  
In Ovo

The use of intensive selection procedure in modern broiler chicken lines has led to the development of several skeletal disorders in broiler chickens. Therefore, current research is focused on methods to improve the bone quality in birds. In ovo technology, using nanoparticles with a high specificity to bones, is a potential approach. The present study aimed to evaluate the effect of in ovo inoculation (IOI) of calcium carbonate nanoparticles (CCN) on chicken embryo development, health status, bone characteristics, and on broiler production results and bone quality. After assessing in vitro cell viability, the IOI procedure was performed with an injection of 500 μg/mL CCN. The control group was not inoculated with CCN. Hatchability, weight, and selected bone and serum parameters were measured in embryos. Part of hatchlings were reared under standard conditions until 42 days, and production results, meat quality, and bone quality of broilers were determined. CCN did not show cytotoxicity to cells and chicken embryo and positively influenced bone parameters of the embryos and of broilers later (calcification) without negatively affecting the production results. Thus, the IOI of CCN could modify the molecular responses at the stage of embryogenesis, resulting in better mineralization, and could provide a sustained effect, thereby improving bone quality in adult birds.

Effects of in ovo injection of Astragalus kahericus polysaccharide on early growth, carcass weights and blood metabolites in broiler chickens

2021 ◽  
pp. 1-7
Author(s):  
Hussin H. El-Fakhrany ◽  
Zenat A. Ibrahim ◽  
Elwy A. Ashour ◽  
Ali Osman ◽  
Mahmoud Alagawany
Keyword(s):  
Broiler Chickens ◽  
Early Growth ◽  
Blood Metabolites ◽  
In Ovo ◽  
In Ovo Injection

scholarly journals Effects of Clostridium butyricum- and Bacillus spp.-Based Potential Probiotics on the Growth Performance, Intestinal Morphology, Immune Responses, and Caecal Microbiota in Broilers

Antibiotics ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 624
Author(s):  
Xinfu Zeng ◽  
Qing Li ◽  
Caimei Yang ◽  
Yang Yu ◽  
Zixian Fu ◽  
...  
Keyword(s):  
Immune Responses ◽  
Growth Performance ◽  
Broiler Chickens ◽  
Basal Diet ◽  
Short Chain Fatty Acids ◽  
Clostridium Butyricum ◽  
Intestinal Morphology ◽  
Treatment Groups ◽  
Body Weights ◽  
Bacillus Spp

We aimed to investigate the effects of Clostridium butyricum-, Bacillus subtilis-, and Bacillus licheniformis-based potential probiotics on the growth performance, intestinal morphology, immune responses, and caecal short chain fatty acids (SCFAs) and microbial structure in broiler chickens. Three treatment groups containing a total of 1200 one-day-old AA broilers were included: birds fed with a basal diet only (Con), birds fed with added 1010 probiotics cfu/kg (ProL), and birds fed with added 1011 probiotics cfu/kg (ProH). The dietary probiotics significantly improved the final and average body weights and serum immunoglobulins A, M, and Y. The probiotics also enhanced the ileal morphology and improved the caecal acetate, butyrate, and propionate contents. Furthermore, 16S rRNA sequencing revealed that dietary compound probiotics modulated the caecal microflora composition as follows: (1) all birds shared 2794 observed taxonomic units; (2) treatment groups were well separated in the PCA and PCoA analysis; (3) the relative abundance of Parabacteroides, Ruminococcaceae_UCG-014, Barnesiella, Odoribacter, [Eubacterium_coprostanoligenes_group], [Ruminococcus]_torques_group, and Butyricimonas significantly varied between treatments. The compound probiotics improved the growth performance, serum immune responses, the ratio of ileal villus height to crypt depth, and major caecal SCFAs in broiler chickens. The dietary C. butyricum-, B. subtilis-, and B. licheniformis-based probiotics improved overall broiler health and would benefit the poultry industry.

Effect of In Ovo Administered Reovirus Vaccines on Immune Responses of Specific-Pathogen-Free Chickens

10.1637/7087 ◽  
2004 ◽  
Vol 48 (1) ◽  
pp. 224-228 ◽  
Author(s):  
Z. Y. Guo ◽  
J. J. Giambrone ◽  
Z. Liu ◽  
T. V. Dormitorio ◽  
Hongzhuan Wu
Keyword(s):  
Immune Responses ◽  
Specific Pathogen Free ◽  
In Ovo ◽  
Specific Pathogen
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