Diagnostic Effectiveness of Electrophoresis and Specific Protein Assays, Evaluated by Discriminate Analysis

1972 ◽  
Vol 18 (2) ◽  
pp. 116-123 ◽  
Author(s):  
Mario Werner ◽  
Samuel H Brooks ◽  
Georg Cohnen
Keyword(s):  
Multivariate Analysis ◽  
Clinical Diagnosis ◽  
Serum Protein ◽  
Protein Pattern ◽  
Paper Electrophoresis ◽  
Specific Protein ◽  
Discriminate Analysis ◽  
Disease Discrimination ◽  
Protein Assays

Abstract With use of the serum protein pattern as a model, we compared the "Diagnostic Effectiveness" of tests of different chemical specificity—i.e., the percentage classified correctly according to the clinical diagnosis. When results obtained from a selected population of subjects with selected diseases were evaluated by multivariate analysis, disease discrimination by paper electrophoresis, which resolves only chemically heterogeneous fractions, was similar to that of a battery of specific assays for individual proteins.

Multivariate Analysis of Serum Protein Assays in Chronic Hepatitis and Postnecrotic Cirrhosis

1974 ◽  
Vol 20 (4) ◽  
pp. 428-429 ◽  
Author(s):  
Angelo Agostoni ◽  
Bianca Marasini ◽  
Roberto Stabilini ◽  
Ersilio Del Ninno ◽  
Mirella Pontello
Keyword(s):  
Multivariate Analysis ◽  
Chronic Hepatitis ◽  
Serum Protein ◽  
Chronic Persistent Hepatitis ◽  
Good Discrimination ◽  
Chronic Aggressive Hepatitis ◽  
Postnecrotic Cirrhosis ◽  
Protein Assays

Abstract Pre-albumin, albumin, orosomucoid, α-antitrypsin, α-macroglobulin, β1A-globulin, IgG, IgA, and IgM were assayed in the serum of patients with chronic persistent hepatitis, chronic aggressive hepatitis, and postnecrotic cirrhosis. When results were evaluated by multivariate analysis, a good discrimination was obtained, especially between chronic aggressive hepatitis and postnecrotic cirrhosis.

EFFECT OF TEMPERATURE AND PHOTOPERIOD ON THE SERUM PROTEINS OF THE RAINBOW TROUT, SALMO GAIRDNERI

1962 ◽  
Vol 40 (2) ◽  
pp. 127-130 ◽  
Author(s):  
Herman M. Meisner ◽  
C. P. Hickman Jr.
Keyword(s):  
Rainbow Trout ◽  
Serum Protein ◽  
Serum Proteins ◽  
Protein Pattern ◽  
Paper Electrophoresis ◽  
Effect Of Temperature ◽  
Salmo Gairdneri

The serum of 1- and 2-year-old rainbow trout, acclimated at least 43 days to four conditions of temperature and daylength (16°–8L (hours daily photoperiod), 16°–16L, 8°–8L, and 8°–16L) was examined by paper electrophoresis. The albumin/globulin ratio was significantly greater in trout acclimated to 8 °C (pooled photoperiod) than in those acclimated to 16 °C (pooled photoperiod). Photoperiod had no significant effect on the serum protein pattern.

Electrophoresis, Specific Protein Assays, or Both in Measurement of Plasma Proteins?

1973 ◽  
Vol 19 (1) ◽  
pp. 99-102 ◽  
Author(s):  
Carl Bertil Laurell
Keyword(s):  
Serum Protein ◽  
Plasma Proteins ◽  
Protein Composition ◽  
Relevant Information ◽  
Specific Protein ◽  
Electrophoretic Separation ◽  
Visual Evaluation ◽  
Specific Analysis ◽  
Cumulative Knowledge ◽  
Protein Assays

Abstract Cumulative knowledge of the protein composition of plasma and newer techniques for specific analysis of proteins have made obsolete most flocculation tests, the albumin:globulin ratio, scanning diagrams, and most determinations of electrophoretic fractions. More clinically relevant information about the serum protein composition is obtained by critical visual evaluation of the protein bands obtained after electrophoretic separation of plasma or serum in nonadsorptive supporting media, if supplementary specific analysis is made of a small number of proteins such as albumin, orosomucoid, haptoglobins, ceruloplasmin, and the immunoglobulins of the three predominant classes

Appropriate sera for calibration and control of specific protein assays

1993 ◽  
Vol 53 ◽  
pp. 13-15 ◽  
Author(s):  
O. Blaabjerg ◽  
M. Blom ◽  
H. Gry ◽  
P. Hyltoft Petersen ◽  
A. Uldall
Keyword(s):  
Specific Protein ◽  
And Control ◽  
Protein Assays

scholarly journals Snail mucus from the mantle and foot of two land snails, Lissachatina fulica and Hemiplecta distincta, exhibits different protein profile and biological activity

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Nattaphop Noothuan ◽  
Kantamas Apitanyasai ◽  
Somsak Panha ◽  
Anchalee Tassanakajon
Keyword(s):  
Biological Activities ◽  
Protein Pattern ◽  
Protein Profile ◽  
Antibacterial Properties ◽  
Biological Properties ◽  
Specific Protein ◽  
Land Snails ◽  
Snail Species ◽  
Significant Difference ◽  
Different Types

Abstract Objective Snails secrete different types of mucus that serve several functions, and are increasingly being exploited for medical and cosmetic applications. In this study, we explored the protein pattern and compared the biological properties of the mucus secreted from the mantle collar and foot of two snail species, Lissachatina fulica and Hemiplecta distincta. Result Protein profile showed a different pattern between the two species and between the two secretory parts. The mantle-specific protein bands were further characterized and among them was an antibacterial protein, achacin. Accordingly, the mucus from the mantle exhibited the higher antibacterial activity than that from the foot in both snail species. The mucus from H. distincta, first reported here, also showed antibacterial properties, but with a lower activity compared to that for L. fulica. Snail mucus also exhibited anti-tyrosinase activity and antioxidant activity but with no significant difference between the foot and mantle mucus. These results indicate some different protein compositions and biological activities of snail slime from the mantle and foot, which might be associated with their specific functions in the animal and are useful for medical applications.

scholarly journals Serum protein electrophoretic pattern in piglets during the early postnatal period

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Csilla Tóthová ◽  
Robert Link ◽  
Petronela Kyzeková ◽  
Oskar Nagy
Keyword(s):  
Serum Protein ◽  
Serum Proteins ◽  
Protein Pattern ◽  
Electrophoretic Pattern ◽  
Postnatal Period ◽  
Early Postnatal Period ◽  
Total Proteins ◽  
Newborn Piglets ◽  
Γ Globulins ◽  
Protein Electrophoretic Pattern

AbstractThe pattern of serum proteins, the typical features of the electrophoretogram in newborn piglets and during their postnatal development is not completely described. Therefore, the aim of this study was to characterize the changes in serum protein electrophoretic pattern and features of the electrophoretograms during the early postnatal period. Significant changes during the monitored period were found in all evaluated parameters (P < 0.001). The most marked changes were observed mainly in the period before weaning. The concentrations of total proteins, albumin and γ-globulins were before colostrum intake low, γ-globulins represented the smallest proportion of protein fractions. The proportion of α1-globulins was after birth a dominant protein fraction. Significant increase of total proteins, α2-, β- and γ-globulins and decrease of α1-globulins was found 2 days after colostrum intake. The albumin and A/G values increased after birth gradually until weaning. After weaning a significant changes were found in absolute concentrations of total protein and albumin, and in relative values of β-globulin fractions. Presented results showed marked developmental alterations in the serum protein pattern in piglets along with the age. The study also brings new knowledge in the field of description of typical features of electrophoretograms in the observed period of piglet’s life.

Parallel electrophoretic fractionation of alkaline phosphatase and serum protein on cellulose acetate strips: clinical evaluation.

1976 ◽  
Vol 22 (2) ◽  
pp. 261-263 ◽  
Author(s):  
A Burlina ◽  
L Galzigna
Keyword(s):  
Cellulose Acetate ◽  
Serum Protein ◽  
Protein Pattern ◽  
Enzymatic Reaction ◽  
Coupling Reaction ◽  
Staining Procedure ◽  
Time Saving ◽  
Solid Agar ◽  
Fast Red ◽  
Agar Substrate

Abstract We describe here a simple, time-saving method for separating alkaline phosphatases on cellulose acetate, with a parallel detection of serum protein pattern. The staining procedure is based on a coupling reaction between the reaction product of alpha-naphthol with Fast Red Violet LB. The enzymatic reaction is done by bringing the cellulose acetate strip containing the phosphatase band into contact with the surface of a solid agar/substrate mixture. The whole procedure requires 80 min. The method has some features in common with other recently proposed procedures but is more reproducible and rapid because of the choice of the reagents and an improved yield of the coupling step.

EFFECTS OF PORCINE IMMUNE GLOBULIN ADMINISTRATION ON THE SURVIVAL AND SERUM PROTEIN COMPOSITION OF COLOSTRUM-DEPRIVED PIGS REARED IN A NON-ISOLATED ENVIRONMENT

1961 ◽  
Vol 41 (2) ◽  
pp. 236-252 ◽  
Author(s):  
B. D. Owen ◽  
J. M. Bell ◽  
C. M. Williams ◽  
R. G. Oakes
Keyword(s):  
Oral Administration ◽  
Serum Protein ◽  
Immune Globulin ◽  
Serum Proteins ◽  
Protein Pattern ◽  
Post Partum ◽  
Protein Composition ◽  
Paper Strip ◽  
Percentage Survival ◽  
Immune Globulins

Five experiments involving 90 newborn colostrum-deprived pigs were conducted in an attempt to develop a method of rearing applicable in a non-isolated environment. Immune globulins, prepared by ammonium sulphate fractionation of porcine serum, and comprised of a mixture of approximately 75 per cent γ-globulin and 25 per cent β-globulin, were administered orally or parenterally in varying amounts and for varying periods of time. In two experiments porcine albumin, in serum or in a semi-purified solution, was provided in addition to the immune globulins. The distribution of serum proteins in the pigs was studied from birth to 12 weeks of age by paper strip electrophoresis.Parenteral administration of immune globulins did not provide an effective passive immunity, nor did oral administration for 1 day post-partum. A marked improvement in survival occurred when oral administration was continued for 5 days, and it was further found that this treatment provided apparently complete protection against infection during the 5-day period of administration. Albumin appeared to further improve survival.These results, together with the relatively poor survival obtained with positive control pigs (nursed 24 hours) suggested a continuing need for a supply of immune globulins in the lumen of the intestinal tract. Presumably these globulins were active as coproantibodies.The percentage survival in pigs weighing 3 pounds or more at birth was substantially higher than in smaller pigs. Mortality in these experiments was usually attributable to colibacillosis.Serum immune globulin levels at 2 days of age in the artificially reared pigs were elevated in proportion to the amount of globulins given. The effect of albumin was to create a serum protein pattern resembling that of suckled pigs. A marked decline in γ-globulin levels from 2 days to approximately 6 weeks was observed.

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