PSVII-13 The Effects of In Ovo Injection of Nicotinamide Riboside on Avian Myogenesis.

Abstract The objective of this study was to determine the effects of in ovo injection of high yield broilers with nicotinamide riboside (NR) on broiler pectoralis major muscle (PMM) development, growth, and mRNA expression. Fertilized Cobb 700 broiler eggs were randomly assigned to 1 of 4 treatments within a 2×2 factorial design with NR dose (DOS; 0 or 2.5 mM) and injection location (LOC; albumen or yolk) as main effects. Eggs were injected with their assigned treatment at d 10 of incubation. Day-15 and -18 embryos and hatched chicks were euthanized, right PMM and muscle fiber morphometric measurements were collected, and left PMM were utilized for mRNA expression analysis of sirtuin-1 and cyclin D1, 2 and 3. There were no LOC×DOS interactions or main effects for d-15 and -18 PMM measurements (P > 0.23). There were LOC×DOS interactions for hatched chick PMM weight and length, and muscle fiber density (P < 0.04). When NR was injected into the albumen, PMM weight decreased (P = 0.02) and length and fiber density were unaffected (P > 0.33), while PMM weight and fiber density increased when NR was injected into the yolk (P < 0.05). There were no LOC×DOS interactions for all genes analyzed for d-15 and 18-embryos (P > 0.23), but NR embryos had greater expression of all genes (P < 0.03) except d-18 cyclin-D2 (P = 0.08). There were LOC×DOS interactions for hatched chick expression of all genes (P < 0.04). There was no difference in sirturin-1 expression when chicks were injected with 0 mM NR, but 2.5-mM yolk chicks had greater expression than 2.5-mM albumen chicks. When NR was injected into the albumen, expression of all cyclins was unaffected (P > 0.08), while expression increased when NR was injected into the yolk (P < 0.01). Increased expression of sirturin-1 and the cyclins may be responsible for NR catalyzed increase in PMM weight and fiber density.

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Effects of in ovo injection of Astragalus kahericus polysaccharide on early growth, carcass weights and blood metabolites in broiler chickens

Animal Biotechnology ◽

10.1080/10495398.2021.1924763 ◽

2021 ◽

pp. 1-7

Author(s):

Hussin H. El-Fakhrany ◽

Zenat A. Ibrahim ◽

Elwy A. Ashour ◽

Ali Osman ◽

Mahmoud Alagawany

Keyword(s):

Broiler Chickens ◽

Early Growth ◽

Blood Metabolites ◽

In Ovo ◽

In Ovo Injection

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In Ovo Injection of GABA Can Help Body Weight Gain at Hatch, Increase Chick Weight to Egg Weight Ratio, and Improve Broiler Heat Resistance

Animals ◽

10.3390/ani11051364 ◽

2021 ◽

Vol 11 (5) ◽

pp. 1364

Author(s):

Chris Major Ncho ◽

Akshat Goel ◽

Chae-Mi Jeong ◽

Mohamed Youssouf ◽

Yang-Ho Choi

Keyword(s):

Antioxidant Capacity ◽

Total Antioxidant Capacity ◽

Antioxidant Status ◽

Body Weight Gain ◽

Weight Ratio ◽

The Other ◽

Mrna Levels ◽

In Ovo ◽

Total Antioxidant ◽

In Ovo Injection

The aim of this study was to explore the outcomes of an in ovo GABA injection in broilers challenged with HS. In Experiment 1, 210 Arbor Acres eggs were allocated to five treatments: no-injection, and in ovo injection of 0.6 mL of 0%, 5%, 10%, or 20% of GABA. Hatchling weight and CWEWR were significantly increased in the 5% GABA group. In ovo, injection of 10% GABA solution caused a significant decrease in plasma cholesterol and increased plasma total antioxidant capacity of hatchlings. Experiment 2 was conducted with 126 fertile Arbor Acres eggs distributed into one of two groups. At 17.5 days of incubation, one received no injection, and the other was fed 0.6 mL of 10% GABA. On day 10, one subgroup (4 replicates * 3 birds) from each treatment was submitted to HS (38 ± 1 °C for 3 h) while the other was kept at a thermoneutral temperature (29 ± 1 °C). An in ovo injection of GABA significantly increased total antioxidant capacity, but reduced malondialdehyde levels, hepatic mRNA levels of HSP70, FAS, and L-FABP with HS. In conclusion, an in ovo GABA injection improves CWEWR and antioxidant status at hatch, and enhances antioxidant status while downregulating the expression of HSP70 and fatty acid metabolism-related genes in young chicks under HS.

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