scholarly journals Identification and complete genome characterization of a novel picornavirus in turkey (Meleagris gallopavo)

2012 ◽  
Vol 93 (10) ◽  
pp. 2171-2182 ◽  
Author(s):  
Ákos Boros ◽  
Csaba Nemes ◽  
Péter Pankovics ◽  
Beatrix Kapusinszky ◽  
Eric Delwart ◽  
...  

Members of the family Picornaviridae are important pathogens of humans and animals, although compared with the thousands of known bird species (>10 000), only a few (n = 11) picornaviruses have been identified from avian sources. This study reports the metagenomic detection and complete genome characterization of a novel turkey picornavirus from faecal samples collected from eight turkey farms in Hungary. Using RT-PCR, both healthy (two of three) and affected (seven of eight) commercial turkeys with enteric and/or stunting syndrome were shown to be shedding viruses in seven (88 %) of the eight farms. The viral genome sequence (turkey/M176/2011/HUN; GenBank accession no. JQ691613) shows a high degree of amino acid sequence identity (96 %) to the partial P3 genome region of a picornavirus reported recently in turkey and chickens from the USA and probably belongs to the same species. In the P1 and P2 regions, turkey/M176/2011/HUN is related most closely to, but distinct from, the kobuviruses and turdivirus 1. Complete genome analysis revealed the presence of characteristic picornaviral amino acid motifs, a potential type II-like 5′ UTR internal ribosome entry site (first identified among avian-origin picornaviruses) and a conserved, 48 nt long ‘barbell-like’ structure found at the 3′ UTR of turkey/M176/2011/HUN and members of the picornavirus genera Avihepatovirus and Kobuvirus. The general presence of turkey picornavirus – a novel picornavirus species – in faecal samples from healthy and affected turkeys in Hungary and in the USA suggests the worldwide occurrence and endemic circulation of this virus in turkey farms. Further studies are needed to investigate the aetiological role and pathogenic potential of this picornavirus in food animals.

2020 ◽  
Vol 81 ◽  
pp. 104236 ◽  
Author(s):  
Gábor Reuter ◽  
Ákos Boros ◽  
Róbert Mátics ◽  
Beatrix Kapusinszky ◽  
Eric Delwart ◽  
...  

2017 ◽  
Vol 5 (16) ◽  
Author(s):  
Feng Zhao ◽  
Ying Sun ◽  
Bingxu Qian ◽  
Xiaorong Zhang ◽  
Yantao Wu

ABSTRACT As we all know, porcine deltacoronavirus was first detected in Hong Kong, China. Here, we report the complete genome sequence of the Chinese porcine deltacoronavirus strain CHN/Tianjin/2016, which was collected and amplified from clinical fecal samples in March of 2016.


2017 ◽  
Vol 198 ◽  
pp. 23-33 ◽  
Author(s):  
Zhong Peng ◽  
Wan Liang ◽  
Yuanguo Wang ◽  
Wenjing Liu ◽  
Hongfeng Zhang ◽  
...  

2019 ◽  
Vol 164 (8) ◽  
pp. 2183-2186
Author(s):  
Qian Wang ◽  
Feng Ji ◽  
Suting Wang ◽  
Xiaojuan Lin ◽  
Zexin Tao ◽  
...  

2014 ◽  
Vol 4 (1) ◽  
Author(s):  
Jingjing Tang ◽  
Zexin Tao ◽  
Zhengrong Ding ◽  
Yong Zhang ◽  
Jie Zhang ◽  
...  

Virus Genes ◽  
2014 ◽  
Vol 48 (3) ◽  
pp. 534-537 ◽  
Author(s):  
Qing-Bin Lu ◽  
Ying Wo ◽  
Hong-Yu Wang ◽  
Xiao-Ai Zhang ◽  
Dou-Dou Huang ◽  
...  

2013 ◽  
Vol 94 (9) ◽  
pp. 2029-2035 ◽  
Author(s):  
Ákos Boros ◽  
Tamás Kiss ◽  
Orsolya Kiss ◽  
Péter Pankovics ◽  
Beatrix Kapusinszky ◽  
...  

Despite the continuously growing number of known avian picornaviruses (family Picornaviridae), knowledge of their genetic diversity in wild birds, especially in long-distance migrant species is very limited. In this study, we report the presence of a novel picornavirus identified from one of 18 analysed faecal samples of an Afro-Palearctic migrant bird, the European roller (Coracias garrulus L., 1758), which is distantly related to the marine-mammal-infecting seal aquamavirus A1 (genus Aquamavirus). The phylogenetic analyses and the low sequence identity (P1 26.3 %, P2 25.8 % and P3 28.4 %) suggest that this picornavirus could be the founding member of a novel picornavirus genus that we have provisionally named ‘Kunsagivirus’, with ‘Greplavirus A’ (strain roller/SZAL6-KuV/2011/HUN, GenBank accession no. KC935379) as the candidate type species.


2015 ◽  
Vol 160 (8) ◽  
pp. 1931-1938 ◽  
Author(s):  
Dam Thi Vui ◽  
To Long Thanh ◽  
Nguyen Tung ◽  
Anchalee Srijangwad ◽  
Thitima Tripipat ◽  
...  

2013 ◽  
Vol 94 (3) ◽  
pp. 570-582 ◽  
Author(s):  
Chao-Ting Xiao ◽  
Luis G. Giménez-Lirola ◽  
Priscilla F. Gerber ◽  
Yong-Hou Jiang ◽  
Patrick G. Halbur ◽  
...  

Many astrovirus (AstV) species are associated with enteric disease, although extraintestinal manifestations in mammalian and avian hosts have also been described. In this study, the prevalence rates of porcine AstV types 1–5 (PAstV1–PAstV5) were investigated using faecal samples from 509 pigs of which 488 (95.9 %) came from farms with a history of diarrhoea. All of the five known PAstV types were found to circulate in pigs in the USA, and co-infection of a single pig with two or more PAstV types was frequently observed. A high overall prevalence of 64.0 % (326/509) of PAstV RNA-positive samples was detected, with 97.2 % (317/326) of the PAstV RNA-positive pigs infected with PAstV4. Further genomic sequencing and characterization of the selected isolates revealed low sequence identities (49.2–89.0 %) with known PAstV strains, indicating novel types or genotypes of PAstV2, PAstV4 and PAstV5. Some new features of the genomes of the PAstVs were also discovered. The first complete genome of a PAstV3 isolate was obtained and showed identities of 50.5–55.3 % with mink AstV and the novel human AstVs compared with 38.4–42.7 % with other PAstV types. Phylogenetic analysis revealed that PAstV1, PAstV2 and PAstV3 were more closely related to AstVs from humans and other animals than to each other, indicating past cross-species transmission and the zoonotic potential of these PAstVs.


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