Identification of the circ_PRKDC/ miR ‐20a‐3p/ RASA1 axis in regulating HaCaT keratinocyte migration

Keratinocyte migration is initiated toward the wound skin barrier as a crucial process in wound healing. However, the migratory machinery used by keratinocytes is relatively unknown. Histamine signaling, including an increase in the Ca2+ signal, mediated the enhanced protein expression and chloride/bicarbonate exchange activity of anion exchanger AE2 in keratinocytes. In this study, we applied an agarose spot assay to induce a vectorial motion. The vectorial stimulation of the histamine-containing agarose spot enhanced the HaCaT keratinocyte migration, compared to non-directional stimulation. AE2 is associated with the vectorial movement of HaCaT keratinocytes. Enhanced expression of AE2 was mainly associated with an increase in Ca2+ and was abolished by the treatment with the Ca2+ chelating agent BAPTA-AM. These findings revealed that the directionality of Ca2+-exerted stimulation can play a prominent role in facilitating migration through the involvement of AE2 as a migratory machinery in HaCaT keratinocytes.

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Neuregulin induces HaCaT keratinocyte migration via Rac1-mediated NADPH-oxidase activation

Journal of Cellular Physiology ◽

10.1002/jcp.22649 ◽

2011 ◽

Vol 226 (11) ◽

pp. 3014-3021 ◽

Cited By ~ 17

Author(s):

Jun-Sub Kim ◽

Eun-Jung Bak ◽

Boung-Chul Lee ◽

Yong-Sun Kim ◽

Jae-Bong Park ◽

...

Keyword(s):

Nadph Oxidase ◽

Keratinocyte Migration ◽

Hacat Keratinocyte ◽

Nadph Oxidase Activation

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HaCaT keratinocyte migration is dependent on epidermal growth factor receptor signaling and glycogen synthase kinase-3α

Experimental Cell Research ◽

10.1016/j.yexcr.2006.05.009 ◽

2006 ◽

Vol 312 (15) ◽

pp. 2791-2805 ◽

Cited By ~ 46

Author(s):

Leeni Koivisto ◽

Guoqiao Jiang ◽

Lari Häkkinen ◽

Bosco Chan ◽

Hannu Larjava

Keyword(s):

Epidermal Growth Factor Receptor ◽

Growth Factor ◽

Epidermal Growth Factor ◽

Glycogen Synthase ◽

Growth Factor Receptor ◽

Glycogen Synthase Kinase ◽

Keratinocyte Migration ◽

Epidermal Growth ◽

Growth Factor Receptor Signaling ◽

Hacat Keratinocyte

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Inhibition of Proinflammatory Cytokines in Cutibacterium acnes-Induced Inflammation in HaCaT Cells by Using Buddleja davidii Aqueous Extract

International Journal of Inflammation ◽

10.1155/2020/8063289 ◽

2020 ◽

Vol 2020 ◽

pp. 1-8

Author(s):

Anh Thu Nguyen ◽

Ki-young Kim

Keyword(s):

Proinflammatory Cytokines ◽

Proinflammatory Cytokine ◽

Treatment Options ◽

Mapk Signaling ◽

Cytokine Expression ◽

Proinflammatory Cytokine Expression ◽

Cutibacterium Acnes ◽

Anti Inflammatory ◽

New Treatment ◽

Hacat Keratinocyte

Acne is an inflammatory skin disorder; although some anti-inflammatory medicines for treating acne are available in a market, they have considerable side effects; therefore, new treatment options are needed. In the present study, among the 16 aqueous extracts of plants collected from Jeju Island in Korea which are used to test anti-inflammatory activity, B. davidii showed the strong decline of the proinflammatory cytokine expression against the inflammatory process caused by C. acnes in Human HaCaT keratinocyte cells. B. davidii downregulated the expression of 57% of COX-2, 41% of iNOS, and proinflammatory cytokines 29% of TNF-α, 32% of IL-1β, 21% of IL-6, and 35% of IL-8. Furthermore, B. davidii inhibited NF-κB and MAPK signaling cascades in keratinocytes that activated by toll-like receptor 2 (TLR-2) in response to C. acnes. Given those results, B. davidii is a potential agent to reduce the proinflammatory cytokine expression against C. acnes-induced inflammation and might provide an alternative to the current medications.

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