Cell Block in Cytological Diagnostics: Review of Preparatory Techniques

2018 ◽  
Vol 62 (4) ◽  
pp. 237-243 ◽  
Author(s):  
Leena Krogerus ◽  
Ivana Kholová
Keyword(s):  
Targeted Therapy ◽  
Study Design ◽  
Molecular Techniques ◽  
Cell Block ◽  
Wide Range ◽  
Blood Clots ◽  
The Future ◽  
Golden Standard ◽  
Agar Method ◽  
Cytological Diagnostics

Objective: The cell block (CB) technique refers to the processing of sediments, blood clots, or grossly visible tissue fragments from cytological specimens into paraffin blocks that can be cut and stained by the same methods used for histopathology. The technique brings additional tissue architectural information. CB can be used for ancillary techniques such as immunocytochemistry and molecular techniques. Study Design: We reviewed the literature on the various preparatory techniques of CBs. Results: There is a wide range of preparatory techniques for CBs and no golden standard for CBs exists: tens of methods are used in various institutions. The majority of the methods are modified in house techniques with a few commercially available kits. The techniques most commonly used are the plasma/thrombin method, the agar method, and commercially available Histogel- and Cellient CB-methods. Dissatisfaction with the cellular yield of the CBs is common. Conclusions: In the CBs, the cytological material is preserved for future use, which is a tremendous advantage in the era of targeted therapy and biobanking. The CB is thus central to the future of cytology: more can be done with less material and with less invasiveness to the patient.

In Vitro Clot Lysis as a Potential Indicator of Thrombus Resistance to Fibrinolysis – Study in Healthy Subjects and Correlation with Blood Fibrinolytic Parameters

1997 ◽  
Vol 77 (04) ◽  
pp. 725-729 ◽  
Author(s):  
Mario Colucci ◽  
Silvia Scopece ◽  
Antonio V Gelato ◽  
Donato Dimonte ◽  
Nicola Semeraro
Keyword(s):  
Plasminogen Activator ◽  
Clot Lysis ◽  
Individual Response ◽  
Plasminogen Activator Inhibitor 1 ◽  
Autologous Plasma ◽  
Wide Range ◽  
Blood Clots ◽  
Physiological Variations ◽  
Pai 1

SummaryUsing an in vitro model of clot lysis, the individual response to a pharmacological concentration of recombinant tissue plasminogen activator (rt-PA) and the influence on this response of the physiological variations of blood parameters known to interfere with the fibrinolytic/thrombolytic process were investigated in 103 healthy donors. 125I-fibrin labelled blood clots were submersed in autologous plasma, supplemented with 500 ng/ml of rt-PA or solvent, and the degree of lysis was determined after 3 h of incubation at 37° C. Baseline plasma levels of t-PA, plasminogen activator inhibitor 1 (PAI-1), plasminogen, α2-anti-plasmin, fibrinogen, lipoprotein (a), thrombomodulin and von Willebrand factor as well as platelet and leukocyte count and clot retraction were also determined in each donor. rt-PA-induced clot lysis varied over a wide range (28-75%) and was significantly related to endogenous t-PA, PAI-1, plasminogen (p <0.001) and age (p <0.01). Multivariate analysis indicated that both PAI-1 antigen and plasminogen independently predicted low response to rt-PA. Surprisingly, however, not only PAI-1 but also plasminogen was negatively correlated with rt-PA-ginduced clot lysis. The observation that neutralization of PAI-1 by specific antibodies, both in plasma and within the clot, did not potentiate clot lysis indicates that the inhibitor, including the platelet-derived form, is insufficient to attenuate the thrombolytic activity of a pharmacological concentration of rt-PA and that its elevation, similarly to the elevation of plasminogen, is not the cause of clot resistance but rather a coincident finding. It is concluded that the in vitro response of blood clots to rt-PA is poorly influenced by the physiological variations of the examined parameters and that factors other than those evaluated in this study interfere with clot dissolution by rt-PA. In vitro clot lysis test might help to identify patients who may be resistant to thrombolytic therapy.

Features of optative modality in Macedonian language (with emphasis on the functionalities of independent da-construction)

2020 ◽  
pp. 301-323
Author(s):  
Natalya I. Kikilo ◽  
Keyword(s):  
Detailed Analysis ◽  
Present Article ◽  
Literary Language ◽  
Natural Context ◽  
Wide Range ◽  
Verb Forms ◽  
Text Types ◽  
The Future ◽  
The Subject ◽  
Ritual Context

In the Macedonian literary language the analytic da-construction used in an independent clause has a wide range of possible modal meanings, the most common of which are imperative and optative. The present article offers a detailed analysis of the semantics and functions of the Macedonian optative da-construction based on fiction and journalistic texts. The first part of the article deals with the specificities of the optative as a category which primarily considers the subject of a wish. In accordance with the semantic characteristics of this category, optative constructions are used in those discourse text types where the speakers are explicitly designated (the most natural context for the optative is the dialogue). The analysis of the Macedonian material includes instances of atypical usage of the optative da-construction, in which the wish of the subject is not apparent and thereby produces new emotional tonalities perceptible to the reader of a fiction/journalistic text. The study describes Macedonian constructions involving two different verb forms: 1) present tense form (da + praes) and 2) imperfective form (da + impf). These constructions formally designate the hypothetical and counterfactual status of the optative situation, respectively. Thus, the examples in the analysis are ordered according to two types of constructions, which reflect the speaker’s view on the probability of the realisation of his/her wish. Unrealistic wishes can be communicated through the present da-construction, while the imperfective construction denotes situations in which the wish can be realised in the future. The second part of the article is devoted to performative optative da-constructions, which express formulas of speech etiquette, wishes and curses. The analysis demonstrates that these constructions lose their magical functions, when used outside of the ritual context, and begin to function as interjections.

scholarly journals Frequency Division Multiplexing of Terahertz Waves Realized by Diffractive Optical Elements

2021 ◽  
Vol 11 (14) ◽  
pp. 6246
Author(s):  
Paweł Komorowski ◽  
Patrycja Czerwińska ◽  
Mateusz Kaluza ◽  
Mateusz Surma ◽  
Przemysław Zagrajek ◽  
...  
Keyword(s):  
Frequency Division Multiplexing ◽  
Diffractive Optical Elements ◽  
Frequency Division ◽  
Terahertz Waves ◽  
Optical Elements ◽  
Telecommunication Systems ◽  
Wide Range ◽  
The Future ◽  
Wireless Telecommunication ◽  
Finite Distance

Recently, one of the most commonly discussed applications of terahertz radiation is wireless telecommunication. It is believed that the future 6G systems will utilize this frequency range. Although the exact technology of future telecommunication systems is not yet known, it is certain that methods for increasing their bandwidth should be investigated in advance. In this paper, we present the diffractive optical elements for the frequency division multiplexing of terahertz waves. The structures have been designed as a combination of a binary phase grating and a converging diffractive lens. The grating allows for differentiating the frequencies, while the lens assures separation and focusing at the finite distance. Designed structures have been manufactured from polyamide PA12 using the SLS 3D printer and verified experimentally. Simulations and experimental results are shown for different focal lengths. Moreover, parallel data transmission is shown for two channels of different carrier frequencies propagating in the same optical path. The designed structure allowed for detecting both signals independently without observable crosstalk. The proposed diffractive elements can work in a wide range of terahertz and sub-terahertz frequencies, depending on the design assumptions. Therefore, they can be considered as an appealing solution, regardless of the band finally used by the future telecommunication systems.

Digital Authoritarianism and the Future of Human Rights

2021 ◽  
pp. 1-27
Author(s):  
Tiberiu Dragu ◽  
Yonatan Lupu
Keyword(s):  
Human Rights ◽  
New Technologies ◽  
Theoretic Model ◽  
Human Rights Abuses ◽  
Human Rights Regime ◽  
Wide Range ◽  
The Future ◽  
Technological Developments ◽  
Game Theoretic ◽  
Strategic Context

Abstract How will advances in digital technology affect the future of human rights and authoritarian rule? Media figures, public intellectuals, and scholars have debated this relationship for decades, with some arguing that new technologies facilitate mobilization against the state and others countering that the same technologies allow authoritarians to strengthen their grip on power. We address this issue by analyzing the first game-theoretic model that accounts for the dual effects of technology within the strategic context of preventive repression. Our game-theoretical analysis suggests that technological developments may not be detrimental to authoritarian control and may, in fact, strengthen authoritarian control by facilitating a wide range of human rights abuses. We show that technological innovation leads to greater levels of abuses to prevent opposition groups from mobilizing and increases the likelihood that authoritarians will succeed in preventing such mobilization. These results have broad implications for the human rights regime, democratization efforts, and the interpretation of recent declines in violent human rights abuses.

Cell Block Optimization: A Comparative Study of Quality Variables in 4 Different Cell Block Methods

2021 ◽  
pp. 1-7
Author(s):  
Marsali Newman ◽  
Matthew Walsh ◽  
Rosemary Jeffrey ◽  
Richard Hiscock
Keyword(s):  
Comparative Study ◽  
Processing Time ◽  
Morphological Features ◽  
Cell Block ◽  
Total Processing Time ◽  
Block Methods ◽  
Molecular Studies ◽  
Agar Method ◽  
Md Anderson ◽  
Ancillary Studies

<b><i>Objective:</i></b> The cell block (CB) is an important adjunct to cytological preparations in diagnostic cytopathology. Optimizing cellular material in the CB is essential to the success of ancillary studies such as immunohistochemistry (IHC) and molecular studies (MS). Our aim was to identify which CB method was most suitable in a variety of specimen types and levels of cellularity. <b><i>Study Design:</i></b> We assessed 4 different CB methods, thrombin clot method (TCM), MD Anderson method (MDAM), gelatin foam method (GFM), and agar method (AM), with descriptive observations and ranking of the methods based on quantity of cells and morphological features. <b><i>Results:</i></b> TCM performed best in ranking for both quantity of cells and morphological features, followed by MDAM, GFM, and AM. Lack of adjuvant in the MDAM resulted in some unique morphological advantages which, however, also resulted in inconsistent performance. In low cellularity cases insufficient cells were frequently identified on slides from MDAM and AM CBs. Technique touch time was similar for all methods, with total processing time being shortest for TCM followed by MDAM, GFM, and AM. <b><i>Conclusions:</i></b> TCM was the most robust CB technique, retaining high scores for ranking of quantity and morphology in a variety of specimen cellularities and specimen types.

scholarly journals Morphometric and Molecular Analyses of Ostertagia leptospicularis Assadov, 1953 from Ruminants: Species Diversity or Host Influence?

Animals ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 182
Author(s):  
Anna Wyrobisz-Papiewska ◽  
Jerzy Kowal ◽  
Elżbieta Łopieńska-Biernat ◽  
Paweł Nosal ◽  
Iwona Polak ◽  
...  
Keyword(s):  
Host Species ◽  
Roe Deer ◽  
Bos Taurus ◽  
Capreolus Capreolus ◽  
Nematode Species ◽  
Molecular Techniques ◽  
Systematic Classification ◽  
Morphological Variations ◽  
Wide Range ◽  
Induced Changes

Ostertagia leptospicularis Assadov, 1953 was formally described in roe deer Capreolus capreolus and has been reported in a wide range of ruminants, including other Cervidae, as well as Bovidae. Nematode specimens derived from various host species exhibit morphological similarity; however, some differences can be observed. It is unclear if this is due to the differential reaction of one nematode species in different host species (i.e., host-induced changes) or because of distinct nematode species in these hosts (i.e., species complex). This paper focuses on specimens resembling O. leptospicularis f. leptospicularis and its closely related species (Ostertagia ostertagi f. ostertagi) collected from various hosts. Morphometric and molecular techniques were applied to assess host-induced changes in nematode morphology and to clarify its systematic classification. There was an overall effect of host species on measurements of nematodes resembling O. leptospicularis (both males and females), but the distinctiveness of the specimens from cattle Bos taurus were highlighted. The results obtained may suggest that the specimens of O. leptospicularis from cattle in Germany and cervids in central Europe belong to different strains. Furthermore, nematodes from the cervid strain appear to circulate within particular host species, which can be seen in the stated morphological variations.

Report on CLEF 2019

2019 ◽  
Vol 53 (2) ◽  
pp. 108-118
Author(s):  
Martin Braschler ◽  
Linda Cappellato ◽  
Fabio Crestani ◽  
Nicola Ferro ◽  
Gundula Heinatz Bürki ◽  
...  
Keyword(s):  
Standard Test ◽  
Future Perspectives ◽  
Research Papers ◽  
Test Collections ◽  
Lessons Learnt ◽  
Wide Range ◽  
The Future ◽  
20Th Anniversary

This is a report on the tenth edition of the Conference and Labs of the Evaluation Forum (CLEF 2019), held from September 9--12, 2019, in Lugano, Switzerland. CLEF was a four day event combining a Conference and an Evaluation Forum. The Conference featured keynotes by Bruce Croft, Yair Neuman, and Miguel Martínez, and presentation of peer reviewed research papers covering a wide range of topics in addition to many posters. The Evaluation Forum consisted to nine Labs: CENTRE, CheckThat, eHealth, eRisk, ImageCLEF, LifeCLEF, PAN, PIR-CLEF, and ProtestNews, addressing a wide range of tasks, media, languages, and ways to go beyond standard test collections. CLEF 2019 marked the 20th anniversary of CLEF, which was celebrated with a dedicated session and a book on the lessons learnt in twenty years of evaluation activities and the future perspectives for CLEF. CLEF 2019 also introduced the Industry Days to further extend the reach and impact of CLEF.

Molecular markers and antioxidant activity in berry crops: Genetic diversity analysis

2012 ◽  
Vol 92 (6) ◽  
pp. 1121-1133 ◽  
Author(s):  
S. C. Debnath ◽  
Y. L. Siow ◽  
J. Petkau ◽  
D. An ◽  
N. V. Bykova
Keyword(s):  
Genetic Diversity ◽  
Antioxidant Activity ◽  
Molecular Markers ◽  
Molecular Techniques ◽  
Diversity Analysis ◽  
Full Potential ◽  
Genetic Diversity Analysis ◽  
Wide Range ◽  
Berry Crops ◽  
Improvement Programs

Debnath, S. C., Siow, Y. L., Petkau, J., An, D. and Bykova, N. V. 2012. Molecular markers and antioxidant activity in berry crops: Genetic diversity analysis. Can. J. Plant Sci. 92: 1121–1133. An improved understanding of important roles of dietary fruits in maintaining human health has led to a dramatic increase of global berry crop production. Berry fruits contain relatively high levels of vitamin C, cellulose and pectin, and produce anthocyanins, which have important therapeutic values, including antitumor, antiulcer, antioxidant and anti-inflammatory activities. There is a need to develop reliable methods to identify berry germplasm and assess genetic diversity/relatedness for dietary properties in berry genotypes for practical breeding purposes through genotype selection in a breeding program for cultivar development, and proprietary-rights protection. The introduction of molecular biology techniques, such as DNA-based markers, allows direct comparison of different genetic materials independent of environmental influences. Significant progress has been made in diversity analysis of wild cranberry, lowbush blueberry, lingonberry and cloudberry germplasm, and in strawberry and raspberry cultivars and advanced breeding lines developed in Canada. Inter simple sequence repeat (ISSR) markers detected an adequate degree of polymorphism to differentiate among berry genotypes, making this technology valuable for cultivar identification and for the more efficient choice of parents in the current berry improvement programs. Although multiple factors affect antioxidant activity, a wide range of genetic diversity has been reported in wild and cultivated berry crops. Diversity analysis based on molecular markers did not agree with those from antioxidant activity. The paper also discusses the issues that still need to be addressed to utilize the full potential of molecular techniques including expressed sequence tag-polymerase chain reaction (EST-PCR) analysis to develop improved environment-friendly berry cultivars suited to the changing needs of growers and consumers.

The Australia and New Zealand v Japan Southern Bluefin Tuna (Jurisdiction and Admissibility) Award of the First Law of the Sea Convention Annex VII Arbitral Tribunal

2001 ◽  
Vol 16 (2) ◽  
pp. 239-293 ◽  
Author(s):  
Barbara Kwiatkowska
Keyword(s):  
New Zealand ◽  
Bluefin Tuna ◽  
Fundamental Principle ◽  
Law Of The Sea ◽  
Arbitral Tribunal ◽  
Compulsory Arbitration ◽  
Wide Range ◽  
The Future ◽  
Questions And Answers ◽  
Southern Bluefin Tuna

AbstractThe Southern Bluefin Tuna (Jurisdiction and Admissihilily) Award of 4 August 2000 marked the first instance of the application of compulsory arbitration under Part XV, Section 2 of the 1982 UN Law of the Sea Convention and of the exercise by the Annex VII Tribunal of la compétence de la compétence pursuant to Article 288(4) over the merits of the instant dispute. The 72-paragraph Award is a decision of pronounced procedural complexity and significant multifaceted impacts of which appreciation requires an in-depth acquaintance with procedural issues of peaceful settlement of disputes in general and the-law-of-the-sea-related disputes in particular. Therefore, the article surveys first the establishment of and the course of proceedings before the five-member Annex VII Arbitral Tribunal, presided over by the immediate former ICJ President, Judge Stephen M. Schwebel, and also comprising Judges Keith, Yamada. Feliciano and Tresselt. Subsequently, the wide range of specific paramount questions and answers of the Tribunal are scrutinised against the background of arguments advanced by the applicants (Australia and New Zealand) and the respondent (Japan) during both written and oral pleadings, including in reliance on the extensive ICJ jurisprudence and treaty practice concerned. On this basis, the article turns to an appraisal of the impacts of the Arbitral Tribunal's paramount holdings and its resultant dismissal of jurisdiction with the scrupulous regard for the fundamental principle of consensuality. Amongst such direct impacts as between the parties to the instant case, the inducements provided by the Award to reach a successful settlement in the future are of particular importance. The Award's indirect impacts concern exposition of the paramount doctrine of parallelism between the umbrella UN Convention and many compatible (fisheries, environmental and other) treaties, as well as of multifaceted, both substantial and procedural effects of that parallelism. All those contributions will importantly guide other courts and tribunals seised in the future under the Convention's Part XV, Section 2.

Future changes in Beijing haze events under different shared socioeconomic pathways

2021 ◽  
Author(s):  
Liang Guo ◽  
Laura Wilcox ◽  
Massimo Bollasina ◽  
Steven Turnock ◽  
Marianne Lund ◽  
...  
Keyword(s):  
Air Pollution ◽  
Global Warming ◽  
Greenhouse Gases ◽  
Weather Patterns ◽  
Greenhouse Gases Emissions ◽  
Wide Range ◽  
The Future ◽  
Aerosol Emissions

&lt;p&gt;The occurrence of severe haze events remains a serious problem in Beijing. Previous studies suggested that the frequency of weather patterns conducive to haze may increase with global warming. The new Shared Socioeconomic Pathways (SSPs) cover a wide range of uncertainties in aerosol and greenhouse gases emissions. Global and Chinese aerosol emissions are projected to decrease in most SSPs, while increases in greenhouse gases and global warming will continue for the rest of the century. The future, therefore, remains unclear.&lt;/p&gt;&lt;p&gt;We quantified the air pollution over Beijing and associated weather patterns using multiple indices calculated from the SSPs&lt;/p&gt;&lt;p&gt;We show that the occurrence of weather patterns conducive to the formation of haze significantly increases by the end of the century due to increases in greenhouse gases. Aerosol reductions also cause an increase in their occurrence, but reduce the severity of haze, and overall reducing aerosol emissions will be beneficial.&lt;/p&gt;

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