Molecular data allow to elucidate the taxonomic placement of some Umbelliferae from Middle Asia and Afghanistan (Pinacantha, Ladyginia, Peucedanum mogoltavicum)

Phytotaxa ◽  
2018 ◽  
Vol 350 (1) ◽  
pp. 42 ◽  
Author(s):  
GALINA V. DEGTJAREVA ◽  
MICHAEL G. PIMENOV ◽  
TAHIR H. SAMIGULLIN

The systematic position of three Apiaceae-Apioideae taxa, Pinacantha porandica, Ladyginia bucharica and Peucedanum mogoltavicum, from Middle Asia and Afghanistan, is clarified based on nrITS DNA sequence data. In the molecular phylogenetic tree, the monotypic Pinacantha is placed in unresolved position within the Ferulinae. Although there is no morphological information on essential characters, we propose a new position of Pinacantha porandica within the genus Ferula. As a result a new combination Ferula porandica is proposed, with a new section Pinacantha to accommodate it. The attribution of Peucedanum mogoltavicum to Ferula has been confirmed, its correct name being Ferula lithophila. The genus Ladyginia should not be included in Ferula, its closest relatives being Mozaffariania and Glaucosciadium from the Glaucosciadium Clade.

2020 ◽  
pp. 200-209
Author(s):  
Sonja Kistenich ◽  
Mika Bendiksby ◽  
Gothamie Weerakoon

The tropical lichen genus Aciculopsora is still very poorly collected. Only eleven collections are known worldwide. We present a molecular phylogenetic tree based on mtSSU and nrITS sequence data from six Aciculopsora specimens. Our results corroborate the monophyly of the genus. We conclude that Aciculopsora consists of three species: A. cinerea, A. longispora comb. nov. (≡ Phyllopsora longispora, = A. salmonea syn. nov.) and A. srilankensis sp. nov. Aciculopsora cinerea occurs in Brazil, A. longispora in Argentina, Costa Rica, Ecuador, and Kenya, and A. srilankensis in Sri Lanka. As such, the genus is new for the Paleotropics, Argentina and Ecuador.


2020 ◽  
Vol 78 ◽  
pp. 33-40
Author(s):  
Hui Geng ◽  
Cheng-De Li ◽  
Jason Mottern ◽  
Andrew Polaszek

Idiococcobius Hayat, syn. nov., is synonymised with Coccobius Ratzeburg, based on morphological and molecular data from a new species of Coccobius from Malaysian Borneo. The new species is sufficiently similar morphologically to the type species of Idiococcobius to place it unambiguously within that genus, but molecular data from the new species, and a reassessment of the morphology of Idiococcobius, indicate synonymy of the two genera. Idiococcobius encarsoides Hayat is therefore transferred to Coccobius; resulting in the new combination: Coccobius encarsoides (Hayat), comb. nov.Coccobius islandicus Geng & Polaszek, sp. nov, is described from morphology and DNA sequence data.


2020 ◽  
Vol 27 (03) ◽  
Author(s):  
VAN HONG THIEN ◽  
TRAN THỊ THU NGAN ◽  
LUU THAO NGUYEN ◽  
TRINH NGOC NAM

Crinum latifolium and C. asiaticum are the two species of genus Crinum, Amaryllidaceae family and they have highly medicinal values. Distinction between two species is challenging due to many similar morphological characteristics. In this study, we used molecular makers to distinguish the two species Crinum latifolium and C. asiaticum. The phylogenetic tree was constructed based on the DNA sequence data of the ITS (internal transcribed spacer) nuclear ribosomal DNA (nrDNA) sequences of Crinum latifolium and C. asiaticum. As a result, C. latifolium and C. asiaticum were sorted into two different groups in the phylogenetic tree. Therefore, C. latifolium and C. asiaticum were carried out to solve the taxonomic ambiguity.


2013 ◽  
Vol 58 (2) ◽  
pp. 467-474 ◽  
Author(s):  
Xiaolan He ◽  
Yu Sun

Abstract The monotypic Pleurocladopsis, endemic to Chile, was established by Schuster in 1964 based on an earlier poorly known species Cephalozia (?) simulans C. Massal. The phylogenetic position of Pleurocladopsis simulans had been considered uncertain until it was placed in the family Schistochilaceae on account of the gynoecial and sporophytic characters. It has been assumed that Pleurocladopsis represents the starting point of evolution in Schistochilaceae. In the present study, the phylogenetic position and taxonomic status of Pleurocladopsis simulans are inferred from phylogenetic analysis of three chloroplast DNA sequence data. The result suggests that the genus was established solely based on the autapomorphic characters, thus obscuring its actual phylogenetic relationship with Schistochila and that these characters are later derived rather than ancestral. The result also confirms that the gynoecial and sporophytic characters are important in taxonomy, but they may be not sufficient at the infrafamilial level and at other lower taxonomic levels. In accordance with the results of the present study, Pleurocladopsis is synonymised with Schistochila, and the new combination Schistochila simulans (C. Massal.) Xiao L. He & Yu Sun is made.


Phytotaxa ◽  
2016 ◽  
Vol 253 (3) ◽  
pp. 179 ◽  
Author(s):  
DAN ZHU ◽  
ZONG-LONG LUO ◽  
DARBHE JAYARAMA BAHT ◽  
ERIC.H.C. MCKENZIE ◽  
ALI H. BAHKALI ◽  
...  

Helminthosporium species from submerged wood in streams in Yunnan Province, China were studied based on morphology and DNA sequence data. Descriptions and illustrations of Helminthosporium velutinum and a new species H. aquaticum are provided. A combined phylogenetic tree, based on SSU, ITS and LSU sequence data, place the species in Massarinaceae, Pleosporales. The polyphyletic nature of Helminthosporium species within Massarinaceae is shown based on ITS sequence data available in GenBank.


2014 ◽  
Vol 62 (3) ◽  
pp. 235 ◽  
Author(s):  
S. Safaei Chaei Kar ◽  
F. Ghanavati ◽  
M. R. Naghavi ◽  
H. Amirabadi-zade ◽  
R. Rabiee

Onobrychis, comprising more than 130 species, is a genus of the family Fabaceae. At this time, the interspecies relationship of this biologically important genus is still a subject of great discussion and debate. To help resolve this disagreement, we used molecular phylogeny to analyse internal transcribed spacer (ITS) and trnL–trnF sequences of 76 species of Onobrychis. Bayesian interference, maximum parsimony and maximum likelihood analyses of nuclear ITS and plastid trnL–trnF DNA sequence data generated trees with strong posterior probability for two groups: Onobrychis subgen. Sisyrosema (including: Heliobrychis, Hymenobrychis, Afghanicae and Anthyllium sections) along with Laxiflorae section in Group I and Onobrychis subgen. Onobrychis (except Laxiflorae section) in the other (Group II). The Laxiflorae section roots back to the ancestral node for Sisyrosema subgen. O. viciifolia (cultivated species), which is closely associated with O. cyri var. cyri, suggesting that the latter may be a wild progenitor of O. viciifolia. The present study supported the paraphyly of subgenera Onobrychis and Sisyrosema. The study proposed the paraphyletic nature of the sections Onobrychis, Dendrobrychis, Heliobrychis and Hymenobrychis. Together with our molecular phylogenetic analyses we present a review of Onobrychis morphology and discuss and compare our results with those of earlier morphological and molecular phylogenetic analyses.


Phytotaxa ◽  
2015 ◽  
Vol 204 (4) ◽  
pp. 253 ◽  
Author(s):  
YONG-YU SU ◽  
YI-LANG HUANG ◽  
LI-JUN CHEN ◽  
PEI-WEN ZHANG ◽  
Zhong-Jian Liu ◽  
...  

A new orchid species, Liparis wenshanensis, discovered in Yunnan, China is described and illustrated in this study based on morphological and molecular analyses. A detailed comparison between the newly discovered orchid and other members of the genus, Liparis, was conducted. The new plant is characterized by the combination of the following features: a long rachis with 45 to 55 flowers; white sepals, petals and column; a greenish lip with a purplish center; strongly recurved and revolute dorsal sepals and petals; strongly recurved, oblong lateral sepals; a cordate lip that is strongly deflexed below the middle, with a two-lobed apex and a two-lobed callus at the base; an arcuate column with a lamella extending along the center almost to the stigma, and with a pair of broad wings toward the apex. These features distinguish the new orchid from all other known species of Liparis. We proceeded to a phylogenetic analysis to ascertain the systematic position of this enigmatic species. Molecular analyses based on nuclear ribosomal ITS and plastid matK DNA sequence data supports the recognition of L. wenshanensis as a distinct species.


2004 ◽  
Vol 17 (2) ◽  
pp. 145 ◽  
Author(s):  
Randall L. Small ◽  
Richard C. Cronn ◽  
Jonathan F. Wendel

Molecular data have had a profound impact on the field of plant systematics, and the application of DNA-sequence data to phylogenetic problems is now routine. The majority of data used in plant molecular phylogenetic studies derives from chloroplast DNA and nuclear rDNA, while the use of low-copy nuclear genes has not been widely adopted. This is due, at least in part, to the greater difficulty of isolating and characterising low-copy nuclear genes relative to chloroplast and rDNA sequences that are readily amplified with universal primers. The higher level of sequence variation characteristic of low-copy nuclear genes, however, often compensates for the experimental effort required to obtain them. In this review, we briefly discuss the strengths and limitations of chloroplast and rDNA sequences, and then focus our attention on the use of low-copy nuclear sequences. Advantages of low-copy nuclear sequences include a higher rate of evolution than for organellar sequences, the potential to accumulate datasets from multiple unlinked loci, and bi-parental inheritance. Challenges intrinsic to the use of low-copy nuclear sequences include distinguishing orthologous loci from divergent paralogous loci in the same gene family, being mindful of the complications arising from concerted evolution or recombination among paralogous sequences, and the presence of intraspecific, intrapopulational and intraindividual polymorphism. Finally, we provide a detailed protocol for the isolation, characterisation and use of low-copy nuclear sequences for phylogenetic studies.


Phytotaxa ◽  
2017 ◽  
Vol 323 (1) ◽  
pp. 27 ◽  
Author(s):  
VLADIMIR E. FEDOSOV ◽  
ALINA V. FEDOROVA ◽  
ELENA A. IGNATOVA ◽  
MICHAEL S. IGNATOV

The genus Seligeria is revised based on morphological and DNA sequence data of nuclear ITS and chloroplastic trnL-F. Fifteen species from most infrageneric units of the genus are recovered in two well supported phylogenetic clusters that are also distinctive in morphology. The clade with the type species of the genus, S. pusilla, includes also S. donniana, S. brevifolia, S. calcarea, S. patula, S. tristichoides, S. trifaria, and S. oelandica. These species are characterized by short, cupulate or turbinate capsules widened towards the mouth, and the lack of a stem central strand. Another clade includes species with rather long, mainly ovate to cylindrical capsules and more or less developed stem central strand: S. campylopoda, S. recurvata, S. subimmersa, S. diversifolia, and S. polaris. These two clusters do not show sister relationships, but the second one appears more closely related to the Blindia clade. To resolve the apparent paraphyly, the latter phylogenetic group is segregated in a genus Blindiadelphus. In some aspects of morphology and ecology it is intermediate between Seligeria s. str. and Blindia, but differs from both genera in subquadrate upper leaf cells and thin- to moderately thick-walled rectangular exothecial cells. Molecular phylogenetic analyses revealed heterogeneity within the specimens previously referred to Blindiadelphus campylopodus, indicating a presence in Asian Russia of an undescribed species that is described here as Blindiadelphus sibiricus. It differs from B. campylopodus by the larger spores and typically rounded leaf apices. The isotype specimen of S. galinae appeared to be nearly identical to S. donniana in the sequences of ITS and trnL-F, and examination of morphology revealed no substantial differences between these species. Thus, we consider S. galinae as a synonym of S. donniana. The genus Blindiadelphus includes species of Seligeria subg. Blindiadelphus and S. subg. Cyrtoseligeria, which however are found intermingled in the molecular phylogenetic analysis. Thus the genus Blindiadelphus is accepted without any infrageneric taxa. The phylogenetic tree is congruent with the subdivision of the genus Seligeria s.str into subg. Seligeria, subg. Anodon, subg. Megalosporia and one newly established subgenus Robustidontia for S. brevifolia.


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