Paphiopedilum charlesworthii var. lannaense, a new slipper orchid from Northern Thailand identified by morphological and AFLP analyses

Phytotaxa ◽  
2022 ◽  
Vol 531 (1) ◽  
pp. 1-17
Author(s):  
WICHARUJ TONGKHAM ◽  
SUPALAK PUMIKONG ◽  
NUTTHA POTAPOHN ◽  
WEENUN BUNDITHYA
Keyword(s):  
Rough Surface ◽  
Molecular Analysis ◽  
Molecular Evidence ◽  
Northern Thailand ◽  
Molecular Analyses ◽  
Yellow Color ◽  
The Family ◽  
Slipper Orchid ◽  
Aflp Technique

A new endemic slipper orchid in the family Orchidaceae from Northern Thailand was described according to morphological and molecular analyses. Morphological information specifies the new slipper orchid Paphiopedilum charlesworthii var. lannaense to be similar to Paphiopedilum charlesworthii (Rolfe) Pfitzer 1895, with the exception of its staminode being obovate-obcordate with yellow color, glittery and rough surface. Molecular analysis by AFLP technique indicates that Paphiopedilum charlesworthii var. lannaense can be included as a member of section Paphiopedilum and is closely related to Paphiopedilum coccineum Perner, H. & Herrmann, R. (2000) (syn. Paphiopedilum barbigerum var. coccineum), from which it differs morphologically by dorsal sepal and petal characters. The morphological and molecular evidence supported that Paphiopedilum charlesworthii var. lannaense is a new slipper orchid in the genus Paphiopedilum (Cypripedioideae, Orchidaceae).

Paphiopedilum notatisepalum, a new species of slipper orchid (Cypripedioideae, Orchidaceae) from China based on morphological and DNA evidence

Phytotaxa ◽  
2017 ◽  
Vol 302 (2) ◽  
pp. 156
Author(s):  
MEINA WANG ◽  
SI-REN LAN ◽  
ZHONG-JIAN LIU
Keyword(s):  
New Species ◽  
Molecular Evidence ◽  
Molecular Analyses ◽  
Pale Yellow ◽  
Dna Evidence ◽  
Green Leaves ◽  
Slipper Orchid ◽  
A New Species

A new species of Paphiopedilum (Orchidaceae) from Yunnan, China, is described and illustrated based on morphological and molecular evidence. Morphological comparisons indicate that the new species P. notatisepalum is highly similar to P. henryanum, from which it differs by its leaves with large yellow spots, shorter scape, larger flower, ovate, white sepals and petals that are pale purple-red with large purple spots and yellow-white margins. Molecular analyses of combined nuclear and plastid datasets (nrITS and matK) indicate that P. notatisepalum is sister to P. barbigerum, which has a green leaves and pale yellow-green sepals and petals. The morphological and molecular evidence support the hypothesis that P. notatisepalum is a new species.

Paphiopedilum erythroanthum, a new species of slipper orchid (Cypripedioideae, Orchidaceae) from China based on morphological and molecular data

Phytotaxa ◽  
2019 ◽  
Vol 406 (5) ◽  
pp. 271-278
Author(s):  
XING-YU LIAO ◽  
DI-YANG ZHANG ◽  
SI-REN LAN ◽  
ZHONG-JIAN LIU
Keyword(s):  
New Species ◽  
Molecular Data ◽  
Molecular Evidence ◽  
Molecular Analyses ◽  
Slipper Orchid ◽  
Morphological And Molecular Data ◽  
A New Species

A new species of Paphiopedilum (Orchidaceae) from Yunnan, China is described and illustrated based on examining morphological and molecular evidence. Morphological comparisons indicate that the new species P. erythroanthum is somewhat similar to P. henryanum, from which it differs by its shorter scape, larger and purple-red flower without purple spots, an obovate dorsal sepal, narrowly oblong petals with recurved apex and a cordate staminode. Molecular analyses of combined nuclear and plastid datasets (ITS and matK) indicate that P. erythroanthum is sister to P. notatisepalum, which has leaves with large yellow spots, a shorter scape, larger flower, white sepals and petals with large purple spots. The morphological and molecular evidence support the hypothesis that P. erythroanthum is a new species.

scholarly journals Astrocoryne cabela, gen. nov. et sp. nov. (Hydrozoa : Sphaerocorynidae), a new sponge-associated hydrozoan

2017 ◽  
Vol 31 (6) ◽  
pp. 734 ◽  
Author(s):  
Davide Maggioni ◽  
Paolo Galli ◽  
Michael L. Berumen ◽  
Roberto Arrigoni ◽  
Davide Seveso ◽  
...  
Keyword(s):  
Red Sea ◽  
New Genus ◽  
Molecular Data ◽  
Molecular Evidence ◽  
New Genus And Species ◽  
Molecular Analyses ◽  
The Family ◽  
Polyp Stage ◽  
Molecular Assessment ◽  
First Time

The family Sphaerocorynidae includes two valid genera and five species, most of which have a confusing taxonomic history. Here, a new genus and species, Astrocoryne cabela, gen. et sp. nov., is described from the Maldives and the Red Sea, based on both morphological and molecular evidence. Astrocoryne cabela has an apomorphy represented by the type of tentacles, here named ‘dicapitate’, and consisting of capitate tentacles with a proximal capitulum-like cluster of nematocysts. Molecular analyses confirmed the monophyly of this species, as well as its belonging to the Sphaerocorynidae, together with Sphaerocoryne spp. and Heterocoryne caribbensis Wedler & Larson, 1986, for which we present molecular data for the first time. Moreover, the high divergence of A. cabela from other species of the family justifies the establishment of a new genus. Interestingly, specimens from the Maldives and the Red Sea showed marked morphological variation in the polyp stage, although only a slight genetic divergence was detected. This study highlights that a comprehensive morpho-molecular assessment of Sphaerocorynidae is strongly needed in order to clarify the taxonomic issues and the diversity of this taxon.

Molecular evidence for non-monophyly of the pinnotheroid crabs (Crustacea : Brachyura : Pinnotheroidea), warranting taxonomic reappraisal

2016 ◽  
Vol 30 (1) ◽  
pp. 1 ◽  
Author(s):  
Emma Palacios Theil ◽  
José A. Cuesta ◽  
Darryl L. Felder
Keyword(s):  
Molecular Phylogeny ◽  
Genetic Markers ◽  
Molecular Genetic ◽  
The Other ◽  
Molecular Evidence ◽  
Generic Level ◽  
Molecular Analyses ◽  
Histone 3 ◽  
The Family ◽  
Molecular Studies

The crabs of the family Pinnotheridae are well known as commensals or parasites, mainly of molluscs and tubeworms. The phylogeny of the group, however, is poorly understood, with preliminary morphological and molecular studies questioning its monophyly. Here we used molecular genetic markers (16S, 12S mitochondrial; histone 3 nuclear) to infer a phylogeny for the family Pinnotheridae De Haan, 1833 to reevaluate the phylogeny and systematics at the level of its subfamilies and genera. Our molecular phylogeny indicated that Parapinnixa cortesi Thoma, Heard, & Vargas, 2005, Parapinnixa hendersoni Rathbun, 1918, Pinnotherelia laevigata H. Milne Edwards & Lucas, 1844, Sakaina yokoyai (Glassell, 1933), Tetrias fischerii (A. Milne-Edwards, 1867) and Tetrias scabripes Rathbun, 1898 should be removed from the family Pinnotheridae, while composition of the present subfamilies, Pinnotherinae De Haan, 1833 and Pinnothereliinae Alcock, 1900, must be revised. At generic level, Clypeasterophilus Campos, 1990, Dissodactylus Smith, 1870, Fabia Dana, 1851, Nepinnotheres Manning, 1993 and Pinnixa White, 1846 were not monophyletic in our analyses. With the exclusion of Pinnotherelia from Pinnotheridae, remaining species of Pinnothereliinae are assigned to Pinnixinae Števčić, 2005, a new subfamily based upon revision and elevation of rank for the tribe Pinnixini Števčić, 2005. In addition, we restructure membership of the subfamily Pinnotherinae and propose Pinnixulalinae, subfam. nov. to accommodate species that were excluded by molecular analyses from the other two subfamilies. These have a firm, wider-than-long carapace with clearly defined regions, strong legs that are usually tuberculate and very setose, and a third maxilliped with an elongate ischiomerus in which the ischium and merus may or may not be indistinguishably fused. Our analyses included 169 pinnotherid exemplars, representing almost half of the genera and about a quarter of the species presently recognised for the family. The relationships within and among some taxa are resolved to greater or lesser extent and the phylogenetic biodiversity of pinnotherid crabs is revealed. However, future publications will most likely result in a further increase in the number of taxa.

Molecular analysis confirms Laurenciella marilzae (Rhodophyta, Rhodomelaceae) in the Mediterranean Sea, a species often misidentified as Laurencia dendroidea

2020 ◽  
Vol 63 (6) ◽  
pp. 527-535
Author(s):  
Donatella Serio ◽  
Giovanni Furnari ◽  
Yola Metti
Keyword(s):  
Mediterranean Sea ◽  
Molecular Analysis ◽  
Molecular Data ◽  
Integrative Approach ◽  
Molecular Analyses ◽  
The Mediterranean ◽  
Laurencia Majuscula

AbstractIt was noted that Mediterranean specimens collected at different stations from around Sicily, Italy and referred to as Laurencia dendroidea (as Laurencia majuscula) were similar to the recently described species Laurenciella marilzae. Presented in this study are the results of an integrative approach using both morphology and molecular data (COI-5P + rbcL) to establish which taxon these specimens should be referred to. Molecular analyses show these specimens belong to Laurenciella, and strongly suggest they are within the species L. marilzae. Morphological examinations of these Mediterranean specimens were also detailed and found to support the conclusion that they belong to L. marilzae.

A REVISED GENERIC SYNOPSIS OF HYACINTHACEAE IN SUB-SAHARAN AFRICA, BASED ON MOLECULAR EVIDENCE, INCLUDING NEW COMBINATIONS AND THE NEW TRIBE PSEUDOPROSPEREAE

2003 ◽  
Vol 60 (3) ◽  
pp. 533-568 ◽  
Author(s):  
J. C. MANNING ◽  
P. GOLDBLATT ◽  
M. F. FAY
Keyword(s):  
Phylogenetic Analysis ◽  
Sub Saharan Africa ◽  
Molecular Evidence ◽  
New Combinations ◽  
Generic Level ◽  
The Family ◽  
Molecular Phylogenetic ◽  
Single Genus ◽  
New Tribe ◽  
Sub Saharan

A revised generic synopsis of sub-Saharan Hyacinthaceae is presented, based on a molecular phylogenetic analysis of the family. Generic rank is accorded only to reciprocally monophyletic clades that can be distinguished by recognizable morphological discontinuities, thereby permitting an appropriate generic assignment of species not included in the analysis. Three subfamilies are recognized within the region. Subfamily Ornithogaloideae, characterized by flattened or angular seeds with tightly adhering testa, is considered to include the single genus Ornithogalum, which is expanded to include the genera Albuca, Dipcadi, Galtonia, Neopatersonia and Pseudogaltonia. Recognizing any of these segregates at generic level renders the genus Ornithogalum polyphyletic, while subdivision of Ornithogalum into smaller, morphologically distinguishable segregates in order to preserve the monophyly of each is not possible. Subfamily Urgineoideae, characterized by flattened or winged seeds with brittle, loosely adhering testa, comprises the two mainland African genera Bowiea and Drimia. The latter is well circumscribed by its deciduous, short-lived perianth and includes the previously recognized genera Litanthus, Rhadamanthus, Schizobasis and Tenicroa. The monotypic Madagascan Igidia is provisionally included in the subfamily as a third genus on the basis of its seeds, pending molecular confirmation of its relationships. Subfamily Hyacinthoideae resolves into three clades, distinguished as tribes Hyacintheae (strictly northern hemisphere and not treated further), Massonieae and Pseudoprospereae tribus nov. Full descriptions and a key to their identification are provided for all genera. New combinations reflecting the generic circumscriptions adopted here are made for most African and all Indian and Madagascan species.

scholarly journals Sixty Years with the Chrysobalanaceae

2020 ◽  
Author(s):  
Ghillean T. Prance
Keyword(s):  
Economic Botany ◽  
Field Studies ◽  
Molecular Evidence ◽  
New Techniques ◽  
Conservation Ecology ◽  
The Past ◽  
New Findings ◽  
The Family ◽  
Molecular Studies ◽  
Economic Use

AbstractA review is given of the studies of Ghillean Prance and associates on the Chrysobalanaceae over the past sixty years. This has focussed on defining the generic boundaries in the family and on monographic work with a worldwide approach to this pantropical family. The importance of field studies for work on monographs and Floras is emphasized. Monographs are still the basis for much work on conservation, ecology and economic botany and are needed as a foundation for molecular studies. The importance of being open to experimenting with new techniques and as a result being willing to change the taxonomy in accordance with new findings is demonstrated and emphasized. The twelve genera of the Chrysobalanaceae at the beginning of this career-long study have now increased to twenty-eight in order to present a much better monophyletic and evolutionary arrangement based on recent molecular evidence. In particular it was necessary to divide and rearrange the originally large genera Parinari and Licania into a number of smaller segregate genera. All known species were included in a worldwide monograph published in 2003. A brief review of the economic use for the family is given.

Assignment of a gene(s) conferring apomixis in Tripsacum to a chromosome arm: cytological and molecular evidence

Genome ◽  
1996 ◽  
Vol 39 (6) ◽  
pp. 1133-1141 ◽  
Author(s):  
B. Kindiger ◽  
D. Bai ◽  
V. Sokolov
Keyword(s):  
Key Words ◽  
Molecular Analysis ◽  
Rflp Markers ◽  
Molecular Evidence ◽  
Addition Lines ◽  
Transfer Genes ◽  
Chromosome Arm ◽  
Successful Transfer ◽  
Apomictic Reproduction

Attempts are underway to locate and transfer genes conferring diplosporous apomixis from Tripsacum to maize. The objective of this study was to evaluate several apomictic and sexual maize–Tripsacum hybrids for the presence or absence of Tripsacum chromosomes, PCR–RAPD generated markers, and RFLP markers that would have an association with apomictic development. Cytological and molecular analysis resulted in the identification of the Tripsacum chromosome arm carrying the gene(s) conferring diplosporous apomixis. Evaluations made on apomictic sexually derived maize + Tripsacum addition lines and an apomictic line possessing a Mz6L–Tr16L translocation were used to establish the location of the gene(s). Results of the study indicate that the successful transfer of a single Tripsacum chromosome is all that is necessary to maintain apomictic reproduction in a maize background. Additional use of this material may facilitate the development of an apomictic maize prototype and the eventual isolation of the gene(s). Key words : apomixis, Tripsacum, mapping, maize.

Molecular analyses of genetic variation and phylogenetic relationship in Indian soap nut ( Sapindus L.) and closely related taxa of the family Sapindaceae

Meta Gene ◽  
2017 ◽  
Vol 13 ◽  
pp. 50-56 ◽  
Author(s):  
Kamalesh S. Mahar ◽  
Lok Man S. Palni ◽  
Shirish A. Ranade ◽  
Veena Pande ◽  
Tikam S. Rana
Keyword(s):  
Genetic Variation ◽  
Phylogenetic Relationship ◽  
Molecular Analyses ◽  
The Family ◽  
Indian Soap

The Phylogenetic Position of Crenea in the Lythraceae Based on Molecular Evidence, and the Transfer of Its Two Species to Ammannia

2021 ◽  
Vol 106 ◽  
pp. 325-339
Author(s):  
Shirley A. Graham ◽  
Peter W. Inglis ◽  
Taciana B. Cavalcanti
Keyword(s):  
Morphological Character ◽  
Phylogenetic Analyses ◽  
Taxonomic Status ◽  
Phylogenetic Position ◽  
Molecular Evidence ◽  
Monophyletic Lineage ◽  
The Family ◽  
Mangrove Vegetation ◽  
Molecular Phylogenetic ◽  
Globally Distributed

Crenea Aubl. (Lythraceae) is a ditypic genus of subshrubs occurring in mangrove vegetation on the coasts of northern South America. Phylogenetic analyses based on morphology have offered unresolved and conflicting phylogenetic positions for the genus in the family. This study presents the first molecular sequences for Crenea, from nrITS, rbcL, trnL, trnL-F, and matK regions. Molecular phylogenetic analyses find full support for Crenea within Ammannia L., a relationship not previously recognized. Ammannia is a globally distributed genus of terrestrial to amphibious herbs mostly occurring in freshwater marshes and wetlands. It was recently reconfigured based on phylogenetic evidence to include the genera Nesaea Comm. ex Kunth and Hionanthera A. Fern. & Diniz. The transfer of Crenea to Ammannia further extends the morphological, ecological, and biogeographical diversity of Ammannia and provides the final evidence defining Ammannia as a monophyletic lineage of the Lythraceae. A revised circumscription of Ammannia s.l. adds several new morphological character states and the first species in the genus restricted to mangrove vegetation. Two changes in taxonomic status are made: Ammannia maritima (Aubl.) S. A. Graham, P. W. Inglis, & T. B. Cavalc., comb. nov., and Ammannia patentinervius (Koehne) S. A. Graham, P. W. Inglis, & T. B. Cavalc., comb. nov. The new combinations are described, a list of exsiccatae examined is provided, and the effects of the reconfiguration to the morphology and biogeography of the genus are detailed.

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