A population-level strain genotyping method to study pathogen strain dynamics in human infections

Mammals house a diversity of bacteria that affect health in various ways, but the routes by which bacterial lineages are transmitted between hosts remain poorly understood. We experimentally determined microbiota transmission modes by deriving 17 inbred mouse lines from two wild populations and monitoring their gut microbiotas for up to 11 host generations. Individual- and population-level microbiota compositions were maintained within mouse lines throughout the experiment, indicating predominantly vertical inheritance of the microbiota. However, certain bacterial taxa tended to be exchanged horizontally between mouse lines. Consistent with evolutionary theory, the degree of horizontal transmission predicted bacterial genera with pathogenic representatives responsible for human infections and hospitalizations.

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The zoonotic potential of bat-borne coronaviruses

Emerging Topics in Life Sciences ◽

10.1042/etls20200097 ◽

2020 ◽

Vol 4 (4) ◽

pp. 365-381

Author(s):

Ny Anjara Fifi Ravelomanantsoa ◽

Sarah Guth ◽

Angelo Andrianiaina ◽

Santino Andry ◽

Anecia Gentles ◽

...

Keyword(s):

Genetic Material ◽

Field Research ◽

Sympatric Species ◽

Animal Origin ◽

Mammalian Host ◽

Past Century ◽

The Past ◽

Novel Host ◽

Horseshoe Bat ◽

Human Infections

Seven zoonoses — human infections of animal origin — have emerged from the Coronaviridae family in the past century, including three viruses responsible for significant human mortality (SARS-CoV, MERS-CoV, and SARS-CoV-2) in the past twenty years alone. These three viruses, in addition to two older CoV zoonoses (HCoV-229E and HCoV-NL63) are believed to be originally derived from wild bat reservoir species. We review the molecular biology of the bat-derived Alpha- and Betacoronavirus genera, highlighting features that contribute to their potential for cross-species emergence, including the use of well-conserved mammalian host cell machinery for cell entry and a unique capacity for adaptation to novel host environments after host switching. The adaptive capacity of coronaviruses largely results from their large genomes, which reduce the risk of deleterious mutational errors and facilitate range-expanding recombination events by offering heightened redundancy in essential genetic material. Large CoV genomes are made possible by the unique proofreading capacity encoded for their RNA-dependent polymerase. We find that bat-borne SARS-related coronaviruses in the subgenus Sarbecovirus, the source clade for SARS-CoV and SARS-CoV-2, present a particularly poignant pandemic threat, due to the extraordinary viral genetic diversity represented among several sympatric species of their horseshoe bat hosts. To date, Sarbecovirus surveillance has been almost entirely restricted to China. More vigorous field research efforts tracking the circulation of Sarbecoviruses specifically and Betacoronaviruses more generally is needed across a broader global range if we are to avoid future repeats of the COVID-19 pandemic.

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Low Control And High Demands At Work And Suicide: An Australian National Population-level Case-control Study

PsycEXTRA Dataset ◽

10.1037/e506802017-001 ◽

2017 ◽

Author(s):

Anthony D. LaMontagne

Keyword(s):

Case Control Study ◽

Population Level ◽

Case Control ◽

National Population ◽

Control Study

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P032 E-CIGARETTE AND CIGARETTE USE AMONG U.S. ADULT IBD PATIENTS: POPULATION-LEVEL SURVEY

Gastroenterology ◽

10.1053/j.gastro.2019.11.242 ◽

2020 ◽

Vol 158 (3) ◽

pp. S102

Author(s):

Ryan Suk ◽

Heetae Suk ◽

Kalyani Sonawane ◽

Ashish Deshmukh

Keyword(s):

Population Level ◽

Cigarette Use

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Cranial crassicaudiasis in two coastal dolphin species from South Africa is predominantly a disease of immature individuals

Diseases of Aquatic Organisms ◽

10.3354/dao03468 ◽

2020 ◽

Vol 139 ◽

pp. 93-102 ◽

Cited By ~ 3

Author(s):

MF Van Bressem ◽

P Duignan ◽

JA Raga ◽

K Van Waerebeek ◽

N Fraijia-Fernández ◽

...

Keyword(s):

South Africa ◽

Bone Development ◽

Bottlenose Dolphins ◽

Fatal Outcome ◽

Population Level ◽

Cape Coast ◽

Osteolytic Lesions ◽

Immune Mediated ◽

Significant Difference ◽

The Difference

Crassicauda spp. (Nematoda) infest the cranial sinuses of several odontocetes, causing diagnostic trabecular osteolytic lesions. We examined skulls of 77 Indian Ocean humpback dolphins Sousa plumbea and 69 Indo-Pacific bottlenose dolphins Tursiops aduncus, caught in bather-protecting nets off KwaZulu-Natal (KZN) from 1970-2017, and skulls of 6 S. plumbea stranded along the southern Cape coast in South Africa from 1963-2002. Prevalence of cranial crassicaudiasis was evaluated according to sex and cranial maturity. Overall, prevalence in S. plumbea and T. aduncus taken off KZN was 13 and 31.9%, respectively. Parasitosis variably affected 1 or more cranial bones (frontal, pterygoid, maxillary and sphenoid). No significant difference was found by gender for either species, allowing sexes to be pooled. However, there was a significant difference in lesion prevalence by age, with immature T. aduncus 4.6 times more likely affected than adults, while for S. plumbea, the difference was 6.5-fold. As severe osteolytic lesions are unlikely to heal without trace, we propose that infection is more likely to have a fatal outcome for immature dolphins, possibly because of incomplete bone development, lower immune competence in clearing parasites or an over-exuberant inflammatory response in concert with parasitic enzymatic erosion. Cranial osteolysis was not observed in mature males (18 S. plumbea, 21 T. aduncus), suggesting potential cohort-linked immune-mediated resistance to infestation. Crassicauda spp. may play a role in the natural mortality of S. plumbea and T. aduncus, but the pathogenesis and population level impact remain unknown.

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Risk stratification of variants of unknown significance (VUS) in monogenic endocrine tumour genes using population-level genetic data and computational analysis

Endocrine Abstracts ◽

10.1530/endoabs.65.p132 ◽

2019 ◽

Author(s):

Hannah Vennard ◽

Cyrielle Maroteau ◽

Jonathan Berg ◽

David Goudie ◽

Colin Palmer ◽

...

Keyword(s):

Risk Stratification ◽

Computational Analysis ◽

Population Level ◽

Genetic Data ◽

Endocrine Tumour ◽

Variants Of Unknown Significance ◽

Unknown Significance

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Population level risk factors associated with childhood obesity

Obesity Abstracts ◽

10.1530/obabs.01.rfc2.6 ◽

2019 ◽

Author(s):

Claire Beynon ◽

Nora Pashyan ◽

Elizabeth Fisher ◽

Dougal Hargreaves ◽

Linda Bailey ◽

...

Keyword(s):

Risk Factors ◽

Childhood Obesity ◽

Population Level ◽

Factors Associated

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Hungarian diet and nutritional status survey – The OTAP2009 study I. Nutritional status of the Hungarian population

Orvosi Hetilap ◽

10.1556/oh.2012.29375 ◽

2012 ◽

Vol 153 (26) ◽

pp. 1023-1030 ◽

Cited By ~ 13

Author(s):

Éva Martos ◽

Viktória Anna Kovács ◽

Márta Bakacs ◽

Csilla Kaposvári ◽

Andrea Lugasi

Keyword(s):

Nutritional Status ◽

Abdominal Obesity ◽

Public Health Problem ◽

Participation Rate ◽

Population Level ◽

Prevalence Of Obesity ◽

Interview Survey ◽

European Health ◽

European Health Interview Survey ◽

And Gender

Obesity is a leading public health problem, but representative data on measured prevalence among Hungarian adults has been missing since the late eighties. Aim and method: Joining in European Health Interview Survey the aim of the OTAP2009 study was to provide data representative by age and gender on the prevalence of obesity and abdominal obesity among Hungarian adults based on their measured anthropometric data. Results: Participation rate was 35% (n = 1165). Data shows that nearly two-thirds of adults are overweight or obese. 26.2% of men and 30.4% of women are obese. Prevalence of morbid obesity is 3.1% and 2.6% in men and women, respectively. Abdominal obesity is more prevalent among women than men (51.0% vs. 33.2%), and rate is increasing parallel with age in both gender. In elderly, 55% of men and almost 80% of women are abdominally obese. Conclusions: Besides interventions of population level for tackling obesity, individual preventive measures are indispensable. Orv. Hetil., 2012, 153, 1023–1030.

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