scholarly journals Composition and Antimicrobial Activity of the Leaf Essential oil of Litsea kostermansii from Taiwan

2009 ◽  
Vol 4 (8) ◽  
pp. 1934578X0900400 ◽  
Author(s):  
Chen-Lung Ho ◽  
Eugene I-Chen Wang ◽  
Kuang-Ping Hsu ◽  
Pei-Yeh Lee ◽  
Yu-Chang Su

The hydrodistillated leaf essential oil of Litsea kostermansii was analyzed to determine its composition and yield. Seventy-three compounds were identified, the main components being β-eudesmol (22.5%), γ-eudesmol (18.6%), δ-selinene (8.5%), α-eudesmol (6.0%), and γ-muurolene (4.7%). Oxygenated sesquiterpenes (66.2%) and sesquiterpene hydrocarbons (32.8%) were the predominant groups of compounds. The leaf oil exhibited excellent antimicrobial activities.

2011 ◽  
Vol 6 (2) ◽  
pp. 1934578X1100600 ◽  
Author(s):  
Chen-Lung Ho ◽  
Kuang-Ping Hsu ◽  
Yen-Hsueh Tseng ◽  
Eugene I-Chen Wang ◽  
Pei-Chun Liao ◽  
...  

The hydrodistillated leaf essential oil of Machilus kusanoi was analyzed to determine its composition and yield. Twenty-three compounds were identified, the main components being β-caryophyllene (23.3%), β-eudesmol (17.1%), α-terpineol (16.0%), n-dodecanal (14.2%), and n-decanal (10.4%). Sesquiterpene hydrocarbons (28.1%) and non-terpenoids (25.0%) were the predominant groups of compounds. The leaf oil exhibited excellent antimicrobial and anti-wood-decay fungal activities.


2009 ◽  
Vol 4 (6) ◽  
pp. 1934578X0900400
Author(s):  
Chen-Lung Ho ◽  
Eugene I-Chen Wang ◽  
Pei-Yeh Lee ◽  
Yu-Chang Su

The leaf essential oil of Litsea nakaii was isolated by hydrodistillation and analyzed to determine its composition and yield. Fifty-five compounds were identified, the main components being α-humulene (15.5%), δ-cadinene (9.2%), ( E)-β-ocimene (8.1%), and δ-selinene (7.1%). The leaf oil exhibited excellent antimicrobial activities.


2016 ◽  
Vol 11 (9) ◽  
pp. 1934578X1601100
Author(s):  
Yu-Chang Su ◽  
Kuan-Ping Hsu ◽  
Chen-Lung Ho

The chemical composition and in vitro anti-inflammatory, antioxidant and antimicrobial activities of the leaf essential oil of Machilus konishii has been investigated. The essential oil was isolated using hydrodistillation in a Clevenger-type apparatus, and characterized by GC–FID and GC–MS. Sixty-six compounds were identified, representing 100% of the oil. The main components identified were α-pinene (33.9%), β-pinene (13.9%), and thymol (12.0%). The leaf oil was able to reduce nitric oxide production by lipopolysaccharide-activated murine macrophages RAW 264.7 without reducing the cell viability. In addition, the leaf oil showed strong antioxidant and antimicrobial activities. The major ingredient of the oil that was responsible for the anti-inflammatory, antioxidant and antimicrobial activities was thymol.


2011 ◽  
Vol 6 (9) ◽  
pp. 1934578X1100600
Author(s):  
Chen-Lung Ho ◽  
Pei-Chun Liao ◽  
Eugene I-Chen Wang ◽  
Yu-Chang Su

The hydrodistillated leaf essential oil of Neolitsea parvigemma was analyzed to determine its composition and yield. Sixty-two compounds were identified, the main components being β-caryophyllene (14.2%), β-eudesmol (12.9%), α-cadinol (10.2%) and τ-cadinol (8.8%). Oxygenated sesquiterpenes (48.9%) and sesquiterpene hydrocarbons (48.8%) were the predominant groups of compounds. The antifungal indexes of the leaf oil against the 7 fungi, Aspergillus clavatus, A. niger, Chaetomium globosum, Cladosporium cladosporioides, Myrothecium verrucaria, Penicillium citrinum and Trichoderma viride, were 100.0, 72.3, 100.0, 100.0, 100.0, 75.8 and 88.6% at a 1 mg/mL concentration, respectively. The oil also exhibited anti-wood-decay-fungi activity against Trametes versicolor, Phaneochaete chrysosporium, Phaeolus schweintizii, and Lenzites sulphureu with MIC values of 50, 50, 25 and 25 μg/mL, respectively. For the antifungal and anti-wood-decay fungal activities of the oil, the active source compounds were determined to be α-cadinol, β-eudesmol and τ-cadinol.


2010 ◽  
Vol 5 (7) ◽  
pp. 1934578X1000500
Author(s):  
Chen-Lung Ho ◽  
Pei-Chun Liao ◽  
Kuang-Ping Hsu ◽  
Eugene I-Chen Wang ◽  
Wei-Chih Dong ◽  
...  

The hydrodistillated leaf essential oil of Machilus pseudolongifolia was analyzed to determine its composition and yield. Seventy compounds were identified, the main components being β-eudesmol (26.8%), α-cadinol (20.8%), viridiflorene (8.9%), α-caryophyllene (5.3%), globulol (4.6%) and β-caryophyllene (4.2%). Oxygenated sesquiterpenes (60.1%) and sesquiterpene hydrocarbons (31.4%) were the predominant groups of compounds. The leaf oil exhibited excellent antimicrobial and anti-wood-decay fungal activities.


2012 ◽  
Vol 7 (1) ◽  
pp. 1934578X1200700
Author(s):  
Chen-Lung Ho ◽  
Yu-Chang Su

The chemical composition, and antioxidant and antimicrobial activities of the essential oil isolated from the leaf of Machilus japonica from Taiwan have been investigated. The essential oil from the fresh leaves was isolated using hydrodistillation in a Clevenger-type apparatus, and characterized by GC-FID and GC-MS. A total of 97 compounds were identified, representing 100% of the oil. The main components identified were α-phellandrene (14.5%), α-pinene (12.8%), thymol (12.6%), β-pinene (8.3%), α-terpineol (6.5%) and carvacrol (6.0%). The antioxidant activity of the oil was tested by the DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical scavenging capability test. The results showed that the IC50 was 51.8 μg/mL. The antimicrobial activity of the oil was tested by the disc diffusion and micro-broth dilution methods against ten microbial species. The oil exhibited strong growth suppression against Gram-positive bacteria and yeast, with inhibition zones of 48~54 mm and MIC values of 16.12~32.25 μg/mL, respectively. For the antioxidant and antimicrobial activities of the oil, the active source compounds were determined to be thymol and carvacrol.


Plants ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 453 ◽  
Author(s):  
Nhan Trong Le ◽  
Duc Viet Ho ◽  
Tuan Quoc Doan ◽  
Anh Tuan Le ◽  
Ain Raal ◽  
...  

The present study aimed to determine the antimicrobial activity and chemical composition of leaves-extracted essential oil of Leoheo domatiophorus Chaowasku, D.T. Ngo and H.T. Le (L. domatiophorus), including antibacterial, antimycotic, antitrichomonas and antiviral effects. The essential oil was obtained using hydrodistillation, with an average yield of 0.34 ± 0.01% (v/w, dry leaves). There were 52 constituents as identified by GC/MS with available authentic standards, representing 96.74% of the entire leaves oil. The essential oil was comprised of three main components, namely viridiflorene (16.47%), (-)-δ-cadinene (15.58%) and γ-muurolene (8.00%). The oil showed good antimicrobial activities against several species: Gram-positive strains: Staphylococcus aureus (two strains) and Enterococcus faecalis, with Minimum Inhibitory Concentration (MIC) and Minimum Lethal Concentration (MLC) values from 0.25 to 1% (v/v); Gram-negative strains such as Escherichia coli (two strains), Pseudomonas aeruginosa (two strains) and Klebsiella pneumoniae, with MIC and MLC values between 2% and 8% (v/v); and finally Candida species, having MIC and MLC between 0.12 and 4% (v/v).Antitrichomonas activity of the oil was also undertaken, showing IC50, IC90 and MLC values of 0.008%, 0.016% and 0.03% (v/v), respectively, after 48h of incubation. The essential oil resultedin being completely ineffective against tested viruses, ssRNA+ (HIV-1, YFV, BVDV, Sb-1, CV-B4), ssRNA- (hRSVA2, VSV), dsRNA (Reo-1), and dsDNA (HSV-1, VV) viruses with EC50 values over 100 µg/mL. This is the first, yet comprehensive, scientific report about the chemical composition and pharmacological properties of the essential oil in L. domatiophorus.


2013 ◽  
Vol 8 (4) ◽  
pp. 1934578X1300800 ◽  
Author(s):  
Yu-Chang Su ◽  
Kuan-Ping Hsu ◽  
Eugene I-Chen Wang ◽  
Chen-Lung Ho

This study investigated the chemical composition and in vitro anticancer activities of the essential oil isolated from the leaf of Neolitsea variabillima. The essential oil was isolated using hydrodistillation in a Clevenger-type apparatus, and characterized by GC-FID and GC-MS. Sixty-seven compounds were identified, representing 100% of the oil. The main components identified were trans-β-ocimene (13.4%), α-cadinol (10.5%), terpinen-4-ol (9.3%), τ-cadinol (9.2%), β-caryophyllene (8.8%), and sabinene (6.7%). The anticancer activities of oil were evaluated. The results showed that the oil exhibited cytotoxic activity against human oral, liver, lung, colon, melanoma, and leukemic cancer cells. The presence of β-caryophyllene, τ-cadinol, and α-cadinol significantly contributed to the anticancer activities of N. variabillima leaf oil.


1970 ◽  
Vol 25 (2) ◽  
pp. 85-89 ◽  
Author(s):  
Jaripa Begum ◽  
M Nazrul Islam Bhuiyan ◽  
Jasmin Uddin Chowdhury ◽  
M Nuzmul Hoque ◽  
M Nural Anwar

The essential oil of Carum carvi L. seeds was screened for its antimicrobial activity against ten pathogenic bacteria and six phytopathogenic fungi. The essential oil showed promising inhibitory activity against all the test bacteria, even at 2 ìl/disc. The minimum inhibitory concentration (MIC, 100-300 ppm) and minimum bactericidal concentration (MBC, 200-400 ppm) values of essential oil were determined. The antifungal screening of the essential oil showed 100% inhibition of radial mycelial growth of all the test fungi at 100 ppm. The MIC and minimum fungicidal concentration (MFC) values were found to vary from 50-300 ppm and 200-400 ppm respectively. The essential oil extracted by hydrodistillation from seeds of C. carvi was analyzed by gas chromatography-mass spectrometry (GC-MS). About 10 compounds had been identified in the seeds oils, accounting for more than 99.7% of the oils. The main components of the seeds oil were thymol (48.20%), o-cymene (19.29%), ϒ-terpinen (17.61%) and trimethylene dichloride (8.81%). Keywords: Antimicrobial activities; Carum carvi; Essential oil; GC-MS; ThymolDOI: http://dx.doi.org/10.3329/bjm.v25i2.4867 Bangladesh J Microbiol, Volume 25, Number 2, December 2008, pp 85-89


2011 ◽  
Vol 347-353 ◽  
pp. 1584-1589 ◽  
Author(s):  
De Qiang Li ◽  
Sheng Hao Pan ◽  
Xi Wu Zhu ◽  
Lin Tan ◽  
Yue Fen Cao

The essential oil was obtained at yield of 0.30% by hydrodistillation from fresh leaf tissue of Solidago canadensis L. collected from Zhejiang Province, China. GC and GC/MS analysis identified 46 components, representing 94.6% of the leaf oil. The main components were (-)germacrene D (28.4%), bornyl acetate (9.2%), D-Limonene (5.0%). The essential oil was tested for cytotoxic activities against three tumor cell lines. The inhibitory concentrations of the essential oil at 50% effect level (IC50) were 68.1 μg/ml for SMMC-7721, 71.4 μg/ml for SGC-7901, and 156.9 μg/ml for Hala, respectively.


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