Composition, Antioxidant, Antimicrobial and Anti-wood-decay Fungal Activities of the Twig Essential Oil of Taiwania cryptomerioides from Taiwan

This study investigated the chemical composition, antioxidant, antimicrobial and anti-wood-decay fungal activities of the essential oil isolated from the twigs of Taiwania cryptomerioides from Taiwan. The essential oil was isolated using hydrodistillation in a Clevenger-type apparatus, and characterized by GC-FID and GC-MS. A total of 35 compounds were identified, representing 100% of the oil. The main components identified were α-cadinol (45.9%), ferruginol (18.9%) and β-eudesmol (10.8%). The antioxidant activity of the oil was tested by the DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical scavenging capability test. The results showed an IC50 of 90.8 ± 0.2 μg/mL. The active source compound was ferruginol. The antimicrobial activity of the oil was tested by the disc diffusion and micro-broth dilution methods against ten microbial species. The oil exhibited strong growth suppression against Gram-positive bacteria and yeast with inhibition zones of 45~52 mm and MIC values of 31.25~62.5 μg/mL, respectively. The anti-wood-decay fungal activity of the oil was also evaluated. The oil demonstrated excellent activity against four wood-decay-fungal species. For the antimicrobial and anti-wood-decay fungal activities of the oil, the active source compounds were determined to be α-cadinol, β-eudesmol and ferruginol.

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Composition, Antioxidant and Antimicrobial Activities of the Leaf Essential Oil of Machilus Japonica from Taiwan

Natural Product Communications ◽

10.1177/1934578x1200700136 ◽

2012 ◽

Vol 7 (1) ◽

pp. 1934578X1200700

Author(s):

Chen-Lung Ho ◽

Yu-Chang Su

Keyword(s):

Essential Oil ◽

Radical Scavenging ◽

Antimicrobial Activities ◽

Disc Diffusion ◽

Gram Positive Bacteria ◽

Leaf Essential Oil ◽

Inhibition Zones ◽

Main Components ◽

Broth Dilution ◽

Antioxidant And Antimicrobial Activities

The chemical composition, and antioxidant and antimicrobial activities of the essential oil isolated from the leaf of Machilus japonica from Taiwan have been investigated. The essential oil from the fresh leaves was isolated using hydrodistillation in a Clevenger-type apparatus, and characterized by GC-FID and GC-MS. A total of 97 compounds were identified, representing 100% of the oil. The main components identified were α-phellandrene (14.5%), α-pinene (12.8%), thymol (12.6%), β-pinene (8.3%), α-terpineol (6.5%) and carvacrol (6.0%). The antioxidant activity of the oil was tested by the DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical scavenging capability test. The results showed that the IC50 was 51.8 μg/mL. The antimicrobial activity of the oil was tested by the disc diffusion and micro-broth dilution methods against ten microbial species. The oil exhibited strong growth suppression against Gram-positive bacteria and yeast, with inhibition zones of 48~54 mm and MIC values of 16.12~32.25 μg/mL, respectively. For the antioxidant and antimicrobial activities of the oil, the active source compounds were determined to be thymol and carvacrol.

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Chemical Composition, Antimicrobial, Antiradical and Anticholinesterase activity of the Essential Oil of Pulicaria stephanocarpa from Soqotra

Natural Product Communications ◽

10.1177/1934578x1200700137 ◽

2012 ◽

Vol 7 (1) ◽

pp. 1934578X1200700

Author(s):

Nasser A. Awadh Ali ◽

Rebecca A. Crouch ◽

Mohamed A. Al-Fatimi ◽

Norbert Arnold ◽

Axel Teichert ◽

...

Keyword(s):

Chemical Composition ◽

Essential Oil ◽

Radical Scavenging ◽

Gram Positive Bacteria ◽

Ache Inhibitory Activity ◽

Leaf Essential Oil ◽

Radical Scavenging Ability ◽

Main Components ◽

Total Oil ◽

Ellman’S Method

The chemical composition of the hydrodistilled leaf essential oil from Pulicaria stephanocarpa Balf Fil was determined by GC-MS analysis, and its antimicrobial, antioxidant and anticholinesterase (AChE) activities were evaluated. Eighty-three compounds were identified representing 97.2% of the total oil. ( E)-Caryophyllene 13.4%, ( E)-nerolidol 8.5%, caryophyllene oxide 8.5%, α-cadinol 8.2% spathulenol 6.8% and τ-cadinol 4.7%, were the main components. Antimicrobial activity of the oil, evaluated using the disc diffusion and broth dilution methods, demonstrated the highest susceptibility on Gram-positive bacteria and Candida albicans. The free radical scavenging ability of the oil was assessed by the DPPH assay to show antiradical activity with IC50 of 330 μg/mL. Moreover, the oil revealed an AChE inhibitory activity of 47% at a concentration of 200 μg/mL using Ellman's method.

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Composition, Anticancer, and Antimicrobial Activities in vitro of the Heartwood Essential Oil of Cunninghamia lanceolata var. konishii from Taiwan

Natural Product Communications ◽

10.1177/1934578x1200700938 ◽

2012 ◽

Vol 7 (9) ◽

pp. 1934578X1200700 ◽

Cited By ~ 5

Author(s):

Yu-Chang Su ◽

Kuan-Ping Hsu ◽

Eugene I-Chen Wang ◽

Chen-Lung Ho

Keyword(s):

Essential Oil ◽

Human Lung ◽

Antimicrobial Activities ◽

Cunninghamia Lanceolata ◽

Gram Positive Bacteria ◽

Inhibition Zones ◽

Oral Cancer Cells ◽

Main Components ◽

Broth Dilution

This study investigated the chemical composition, anticancer, and antimicrobial activities in vitro of the essential oil isolated from the heartwood of Cunninghamia lanceolata var. konishii from Taiwan. The essential oil was isolated using hydrodistillation in a Clevenger-type apparatus, and characterized by GC-FID and GC-MS. Thirty-seven compounds were identified, representing 100% of the oil. The main components identified were cedrol (58.3%), α-cedrene (11.8%), α-terpineol (4.2%) and β-cedrene (3.5%). The oil exhibited cytotoxic activity against human lung, liver and oral cancer cells. The active source compound was cedrol. The antimicrobial activity of the oil was tested by the disc diffusion and micro-broth dilution methods against ten microbial species. The oil exhibited strong growth suppression against Gram-positive bacteria and yeast with inhibition zones of 42~50 mm to MIC values of 31.25~62.5 μg/mL, respectively. For the antimicrobial activities of the oil, the active compound was determined to be cedrol.

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Composition, in-vitro Anticancer, and Antimicrobial Activities of the Leaf Essential Oil of Machilus mushaensis from Taiwan

Natural Product Communications ◽

10.1177/1934578x1300800236 ◽

2013 ◽

Vol 8 (2) ◽

pp. 1934578X1300800

Author(s):

Yu-Chang Su ◽

Chen-Lung Ho

Keyword(s):

Essential Oil ◽

Antimicrobial Activities ◽

Disc Diffusion ◽

Gram Positive Bacteria ◽

Moderate Growth ◽

Leaf Essential Oil ◽

Inhibition Zones ◽

Main Components ◽

Broth Dilution

This study investigated the chemical composition, in-vitro anticancer, and antimicrobial activities of the essential oil isolated from the leaf of Machilus mushaensis from Taiwan. The essential oil was isolated using hydrodistillation in a Clevenger-type apparatus, and characterized by GC-FID and GC-MS. Forty compounds were identified, representing 100% of the oil. The main components identified were n-decanal (61.0%), and α-cadinol (20.8%). The oil exhibited cytotoxic activity against human oral, liver, lung, colon, melanoma, and leukemic cancer cells. The antimicrobial activity of the oil was tested by the disc diffusion and micro-broth dilution methods against ten microbial species. The oil exhibited moderate growth suppression against Gram-positive bacteria and yeast with inhibition zones of 25-29 mm to MIC values of 375-500 μg/mL, respectively. α-Cadinol was found to show promising anticancer and antimicrobial activities.

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Chemical Constituents of the Essential Oil from Ecuadorian Endemic Species Croton ferrugineus and Its Antimicrobial, Antioxidant and α-Glucosidase Inhibitory Activity

Molecules ◽

10.3390/molecules26154608 ◽

2021 ◽

Vol 26 (15) ◽

pp. 4608

Author(s):

Eduardo Valarezo ◽

Génesis Gaona-Granda ◽

Vladimir Morocho ◽

Luis Cartuche ◽

James Calva ◽

...

Keyword(s):

Gas Chromatography ◽

Essential Oil ◽

Endemic Species ◽

Chemical Constituents ◽

Radical Scavenging ◽

Moderate Activity ◽

Gram Positive Bacteria ◽

Flame Ionization ◽

Total Composition ◽

Radical Scavenging Properties

Croton ferrugineus Kunth is an endemic species of Ecuador used in traditional medicine both for wound healing and as an antiseptic. In this study, fresh Croton ferrugineus leaves were collected and subjected to hydrodistillation for extraction of the essential oil. The chemical composition of the essential oil was determined by gas chromatography equipped with a flame ionization detector and gas chromatography coupled to a mass spectrometer using a non-polar and a polar chromatographic column. The antibacterial activity was assayed against three Gram-positive bacteria, one Gram-negative bacterium and one dermatophyte fungus. The radical scavenging properties of the essential oil was evaluated by means of DPPH and ABTS assays. The chemical analysis allowed us to identify thirty-five compounds representing more than 99.95% of the total composition. Aliphatic sesquiterpene hydrocarbon trans-caryophyllene was the main constituent with 20.47 ± 1.25%. Other main compounds were myrcene (11.47 ± 1.56%), β-phellandrene (10.55 ± 0.02%), germacrene D (7.60 ± 0.60%), and α-humulene (5.49 ± 0.38%). The essential oil from Croton ferrugineus presented moderate activity against Candida albicans (ATCC 10231) with an MIC of 1000 μg/mL, a scavenging capacity SC50 of 901 ± 20 µg/mL with the ABTS method, and very strong antiglucosidase activity with an IC50 of 146 ± 20 µg/mL.

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Chemical Composition and Antimicrobial Activity of Anthriscus nemorosa Root Essential Oil

Natural Product Communications ◽

10.1177/1934578x1100600229 ◽

2011 ◽

Vol 6 (2) ◽

pp. 1934578X1100600

Author(s):

Milica Pavlović ◽

Silvana Petrović ◽

Marina Milenković ◽

Maria Couladis ◽

Olga Tzakou ◽

...

Keyword(s):

Antimicrobial Activity ◽

Essential Oil ◽

Gram Positive Bacteria ◽

Microdilution Method ◽

Broth Microdilution Method ◽

Bacillus Subtilis Atcc ◽

Germacrene D ◽

Main Components ◽

Total Oil ◽

Root Essential Oil

The essential oil obtained by hydrodistillation from the roots of Anthriscus nemorosa (Bieb.) Sprengel (Umbelliferae) was analyzed by GC and GC-MS. Among sixty-two compounds identified (representing 89.0% of the total oil), the main components were: n-nonane (12.1%), n-hexadecanol (6.9%), δ-cadinene (6.4%), β-pinene (6.0%) and germacrene D (5.4%). Furthermore, the antimicrobial activity of the oil was evaluated against the Gram-positive bacteria Staphylococcus epidermidis (ATCC 12228) and Bacillus subtilis (ATCC 6633), the Gramnegative bacterium Escherichia coli (ATCC 25922), and a yeast Candida albicans (ATCC 10259 and ATCC 24433) using the broth microdilution method.

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The Composition, Anti-mildew and Anti-wood-decay Fungal Activities of the Leaf and Fruit Oils of Juniperus Formosana from Taiwan

Natural Product Communications ◽

10.1177/1934578x1300800936 ◽

2013 ◽

Vol 8 (9) ◽

pp. 1934578X1300800 ◽

Cited By ~ 4

Author(s):

Yu-Chang Su ◽

Kuan-Ping Hsu ◽

Eugene I-Chen Wang ◽

Chen-Lung Ho

Keyword(s):

Essential Oil ◽

Wood Decay ◽

Decay Fungi ◽

Active Source ◽

Wood Decay Fungi ◽

Leaf Oil ◽

Fruit Oil ◽

Type Apparatus

In this study, anti-mildew and anti-wood-decay fungal activities of the leaf and fruits essential oil and its constituents from Juniperus formosana were evaluated in vitro against seven mildew fungi and four wood decay fungi, respectively. The main compounds responsible for the anti-mildew and anti-wood-decay fungal activities were also identified. The essential oil from the fresh leaves and fruits of J. formosana were isolated using hydrodistillation in a Clevenger-type apparatus, and characterized by GC-FID and GC-MS, respectively. The leaf oil mainly consisted of α-pinene (41.0%), limonene (11.5%), α-cadinol (11.0%), elemol (6.3%), and β-myrcene (5.8%); the fruit oil was mostly α-pinene (40.9%), β-myrcene (32.4%), α-thujene (5.9%) and limonene (5.9%). Comparing the anti-mildew and anti-wood-decay fungal activities of the oils suggested that the leaf oil was the most effective. For the anti-mildew and anti-wood-decay fungal activities of the leaf oil, the active source compounds were determined to be α-cadinol and elemol.

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Composition and Antimicrobial Activities of the Leaf Essential Oil of Machilus kusanoi from Taiwan

Natural Product Communications ◽

10.1177/1934578x1100600228 ◽

2011 ◽

Vol 6 (2) ◽

pp. 1934578X1100600 ◽

Cited By ~ 2

Author(s):

Chen-Lung Ho ◽

Kuang-Ping Hsu ◽

Yen-Hsueh Tseng ◽

Eugene I-Chen Wang ◽

Pei-Chun Liao ◽

...

Keyword(s):

Essential Oil ◽

Wood Decay ◽

Antimicrobial Activities ◽

Sesquiterpene Hydrocarbons ◽

Leaf Oil ◽

Leaf Essential Oil ◽

Main Components

The hydrodistillated leaf essential oil of Machilus kusanoi was analyzed to determine its composition and yield. Twenty-three compounds were identified, the main components being β-caryophyllene (23.3%), β-eudesmol (17.1%), α-terpineol (16.0%), n-dodecanal (14.2%), and n-decanal (10.4%). Sesquiterpene hydrocarbons (28.1%) and non-terpenoids (25.0%) were the predominant groups of compounds. The leaf oil exhibited excellent antimicrobial and anti-wood-decay fungal activities.

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Chemical Composition, and Cytotoxic, Antioxidant and Antibacterial Activities of the Essential Oil from Ginseng Leaves

Natural Product Communications ◽

10.1177/1934578x1400900637 ◽

2014 ◽

Vol 9 (6) ◽

pp. 1934578X1400900 ◽

Cited By ~ 4

Author(s):

Rui Jiang ◽

Liwei Sun ◽

Yanbing Wang ◽

Jianzeng Liu ◽

Xiaodan Liu ◽

...

Keyword(s):

Essential Oil ◽

Biological Activities ◽

Radical Scavenging ◽

Complex Mixture ◽

Annexin V ◽

Antibacterial Activities ◽

Cytotoxic Activities ◽

Dependent Manner ◽

Concentration Dependent Manner ◽

Gram Positive Bacteria

Panax ginseng C.A.Meyer is one of the most valuable traditional Chinese medicines. In this study, the essential oil of ginseng leaves (EOGL), collected using hydrodistillation and analyzed by GC/MS, contained a complex mixture of aliphatic (69.0%), terpenoid (21.5%) and aromatic compounds (2.4%). Among 54 components identified, the major ones were palmitic acid (36.1%), β-farnesene (15.4%), linoleic acid (9.8%) and phytol (5.6%). In the cytotoxicity study, EOGL exhibited obvious cytotoxic activities against different cancer cell lines, including Hela, A549, ZR-75-1, HT-29, SGC7901 and B16 cells. Furthermore, Annexin V-FITC/PI staining assay indicated that EOGL can induce late apoptosis of ZR-75-1 cells, and the percentage of apoptotic cells increased in a concentration-dependent manner (0.9% to 5.6% and 67.4%). In addition to this, we also found that EOGL exhibited weak DPPH radical scavenging (12.0 ± 0.4 mg/mL) and ABTS radical scavenging activities (1.6 ± 0.1 mg/mL), and showed antibacterial activity against the Gram-positive bacteria, Staphylococcus aureus and Bacillus subtilis, and the Gram-negative bacterium, Escherichia coli. The data suggest that EOGL, which possesses important biological activities, especially significant anticancer activity, could be a potential medicinal resource.

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Antifungal and Antioxidant Activities of the Essential Oil fromAngelica koreanaNakai

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2014/398503 ◽

2014 ◽

Vol 2014 ◽

pp. 1-7 ◽

Cited By ~ 6

Author(s):

Junghyun Roh ◽

Seungwon Shin

Keyword(s):

Essential Oil ◽

Antioxidant Activities ◽

Radical Scavenging ◽

Reducing Power ◽

Minimum Effective Dose ◽

Minimal Inhibitory Concentrations ◽

Oil Fraction ◽

Antioxidant Agents ◽

Main Components ◽

Promising Source

Purpose. The purpose of this study is to determine the antifungal and antioxidant activities of the essential oil fromAngelica koreana.Methods. Essential oil was obtained from the dried roots ofA. koreanaby steam distillation, and its composition was identified by gas chromatography and mass spectrometry (GC-MS). The minimal inhibitory concentrations (MICs) of the oil fraction and its main components were determined by broth dilution assay using common pathogenicAspergillusandTrichophytonspecies. The combined effects of the oils with itraconazole were evaluated using a checkerboard titer test. In addition, 1,1-diphenyl-2-picryl-hydrazil (DPPH) free radical scavenging, nitrite inhibition, and reducing power were determined to assess the antioxidant activity of this oil.Results. The essential oil fraction and its main components showed inhibitory activity against all of the tested fungi, with minimal inhibitory concentrations (MICs) of 250–1000 μg/mL. Furthermore, this oil exhibited synergism when combined with itraconazole.Conclusion. In the treatment of infections caused byAspergillusandTrichophytonspecies, combining itraconazole with eitherA. koreanaessential oil or its main components may reduce the minimum effective dose of itraconazole required and, thus, minimize its side effects. In addition, this oil is a promising source of natural antioxidant agents.

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