Composition, in vitro Cytotoxic, and Antimicrobial Activities of the Flower Essential Oil of Diospyros discolor from Taiwan

This study investigated the chemical composition, in vitro cytotoxic, and antimicrobial activities of the essential oil isolated from the flower of Diospyros discolor from Taiwan. The essential oil was isolated using hydrodistillation in a Clevenger-type apparatus, and characterized by GC-FID and GC-MS. Twenty-eight compounds were identified, representing 100% of the oil. The main components identified were (2 Z,6 E)-farnesol (35.0%), α-cadinol (10.9%), ( E)-nerolidol (6.9%), α-humulene (6.0%), τ-cadinol (5.6%), β-caryophyllene (5.3%), and τ-muurolol (5.3%). The oil exhibited cytotoxic activity against human colon, liver, and lung cancer cells. The active compounds were β-caryophyllene, α-humulene, τ-cadinol, τ-muurolol, α-cadinol, and (2 Z,6 E)-farnesol. The antimicrobial activity of the oil was tested by the disc diffusion and micro-broth dilution methods against ten microbial species. The oil exhibited moderate to strong growth suppression against Bacillus cereus, Staphylococcus aureus, S. epidermidis, Escherichia coli, Enterobacter aerogenes, and Candida albicans, with inhibition zones of 40 to 52 mm and MIC values of 31.25–62.5 μg/mL, respectively. For the antimicrobial activities of the oil, the active compounds were determined to be α-cadinol, τ-cadinol, τ-muurolol and (2 Z,6 E)-farnesol.

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Composition, in-vitro Anticancer, and Antimicrobial Activities of the Leaf Essential Oil of Machilus mushaensis from Taiwan

Natural Product Communications ◽

10.1177/1934578x1300800236 ◽

2013 ◽

Vol 8 (2) ◽

pp. 1934578X1300800

Author(s):

Yu-Chang Su ◽

Chen-Lung Ho

Keyword(s):

Essential Oil ◽

Antimicrobial Activities ◽

Disc Diffusion ◽

Gram Positive Bacteria ◽

Moderate Growth ◽

Leaf Essential Oil ◽

Inhibition Zones ◽

Main Components ◽

Broth Dilution

This study investigated the chemical composition, in-vitro anticancer, and antimicrobial activities of the essential oil isolated from the leaf of Machilus mushaensis from Taiwan. The essential oil was isolated using hydrodistillation in a Clevenger-type apparatus, and characterized by GC-FID and GC-MS. Forty compounds were identified, representing 100% of the oil. The main components identified were n-decanal (61.0%), and α-cadinol (20.8%). The oil exhibited cytotoxic activity against human oral, liver, lung, colon, melanoma, and leukemic cancer cells. The antimicrobial activity of the oil was tested by the disc diffusion and micro-broth dilution methods against ten microbial species. The oil exhibited moderate growth suppression against Gram-positive bacteria and yeast with inhibition zones of 25-29 mm to MIC values of 375-500 μg/mL, respectively. α-Cadinol was found to show promising anticancer and antimicrobial activities.

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Composition, Antioxidant and Antimicrobial Activities of the Leaf Essential Oil of Machilus Japonica from Taiwan

Natural Product Communications ◽

10.1177/1934578x1200700136 ◽

2012 ◽

Vol 7 (1) ◽

pp. 1934578X1200700

Author(s):

Chen-Lung Ho ◽

Yu-Chang Su

Keyword(s):

Essential Oil ◽

Radical Scavenging ◽

Antimicrobial Activities ◽

Disc Diffusion ◽

Gram Positive Bacteria ◽

Leaf Essential Oil ◽

Inhibition Zones ◽

Main Components ◽

Broth Dilution ◽

Antioxidant And Antimicrobial Activities

The chemical composition, and antioxidant and antimicrobial activities of the essential oil isolated from the leaf of Machilus japonica from Taiwan have been investigated. The essential oil from the fresh leaves was isolated using hydrodistillation in a Clevenger-type apparatus, and characterized by GC-FID and GC-MS. A total of 97 compounds were identified, representing 100% of the oil. The main components identified were α-phellandrene (14.5%), α-pinene (12.8%), thymol (12.6%), β-pinene (8.3%), α-terpineol (6.5%) and carvacrol (6.0%). The antioxidant activity of the oil was tested by the DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical scavenging capability test. The results showed that the IC50 was 51.8 μg/mL. The antimicrobial activity of the oil was tested by the disc diffusion and micro-broth dilution methods against ten microbial species. The oil exhibited strong growth suppression against Gram-positive bacteria and yeast, with inhibition zones of 48~54 mm and MIC values of 16.12~32.25 μg/mL, respectively. For the antioxidant and antimicrobial activities of the oil, the active source compounds were determined to be thymol and carvacrol.

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Composition, Anticancer, and Antimicrobial Activities in vitro of the Heartwood Essential Oil of Cunninghamia lanceolata var. konishii from Taiwan

Natural Product Communications ◽

10.1177/1934578x1200700938 ◽

2012 ◽

Vol 7 (9) ◽

pp. 1934578X1200700 ◽

Cited By ~ 5

Author(s):

Yu-Chang Su ◽

Kuan-Ping Hsu ◽

Eugene I-Chen Wang ◽

Chen-Lung Ho

Keyword(s):

Essential Oil ◽

Human Lung ◽

Antimicrobial Activities ◽

Cunninghamia Lanceolata ◽

Gram Positive Bacteria ◽

Inhibition Zones ◽

Oral Cancer Cells ◽

Main Components ◽

Broth Dilution

This study investigated the chemical composition, anticancer, and antimicrobial activities in vitro of the essential oil isolated from the heartwood of Cunninghamia lanceolata var. konishii from Taiwan. The essential oil was isolated using hydrodistillation in a Clevenger-type apparatus, and characterized by GC-FID and GC-MS. Thirty-seven compounds were identified, representing 100% of the oil. The main components identified were cedrol (58.3%), α-cedrene (11.8%), α-terpineol (4.2%) and β-cedrene (3.5%). The oil exhibited cytotoxic activity against human lung, liver and oral cancer cells. The active source compound was cedrol. The antimicrobial activity of the oil was tested by the disc diffusion and micro-broth dilution methods against ten microbial species. The oil exhibited strong growth suppression against Gram-positive bacteria and yeast with inhibition zones of 42~50 mm to MIC values of 31.25~62.5 μg/mL, respectively. For the antimicrobial activities of the oil, the active compound was determined to be cedrol.

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In Vitro Antimicrobial Activity of Essential Oil Extracted from Leaves of Leoheo domatiophorus Chaowasku, D.T. Ngo and H.T. Le in Vietnam

Plants ◽

10.3390/plants9040453 ◽

2020 ◽

Vol 9 (4) ◽

pp. 453 ◽

Cited By ~ 8

Author(s):

Nhan Trong Le ◽

Duc Viet Ho ◽

Tuan Quoc Doan ◽

Anh Tuan Le ◽

Ain Raal ◽

...

Keyword(s):

Antimicrobial Activity ◽

Chemical Composition ◽

Essential Oil ◽

Inhibitory Concentration ◽

Antimicrobial Activities ◽

Antiviral Effects ◽

Scientific Report ◽

Main Components ◽

Hiv 1

The present study aimed to determine the antimicrobial activity and chemical composition of leaves-extracted essential oil of Leoheo domatiophorus Chaowasku, D.T. Ngo and H.T. Le (L. domatiophorus), including antibacterial, antimycotic, antitrichomonas and antiviral effects. The essential oil was obtained using hydrodistillation, with an average yield of 0.34 ± 0.01% (v/w, dry leaves). There were 52 constituents as identified by GC/MS with available authentic standards, representing 96.74% of the entire leaves oil. The essential oil was comprised of three main components, namely viridiflorene (16.47%), (-)-δ-cadinene (15.58%) and γ-muurolene (8.00%). The oil showed good antimicrobial activities against several species: Gram-positive strains: Staphylococcus aureus (two strains) and Enterococcus faecalis, with Minimum Inhibitory Concentration (MIC) and Minimum Lethal Concentration (MLC) values from 0.25 to 1% (v/v); Gram-negative strains such as Escherichia coli (two strains), Pseudomonas aeruginosa (two strains) and Klebsiella pneumoniae, with MIC and MLC values between 2% and 8% (v/v); and finally Candida species, having MIC and MLC between 0.12 and 4% (v/v).Antitrichomonas activity of the oil was also undertaken, showing IC50, IC90 and MLC values of 0.008%, 0.016% and 0.03% (v/v), respectively, after 48h of incubation. The essential oil resultedin being completely ineffective against tested viruses, ssRNA+ (HIV-1, YFV, BVDV, Sb-1, CV-B4), ssRNA- (hRSVA2, VSV), dsRNA (Reo-1), and dsDNA (HSV-1, VV) viruses with EC50 values over 100 µg/mL. This is the first, yet comprehensive, scientific report about the chemical composition and pharmacological properties of the essential oil in L. domatiophorus.

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Chemical Composition and Biological Activity of the Essential Oil from Jamaican Cleome serrata

Natural Product Communications ◽

10.1177/1934578x1200700934 ◽

2012 ◽

Vol 7 (9) ◽

pp. 1934578X1200700 ◽

Cited By ~ 1

Author(s):

Megil J. McNeil ◽

Roy B. R. Porter ◽

Lawrence A. D. Williams

Keyword(s):

Chemical Composition ◽

Essential Oil ◽

Antimicrobial Activities ◽

Diffusion Method ◽

Oil Composition ◽

Filter Paper Disc ◽

Aerial Parts ◽

Main Components ◽

Total Oil

The chemical composition of the essential oil obtained from the aerial parts of Cleome serrata by hydrodistillation was analyzed by employing GC-FID, GC-MS and RI. Fourteen compounds comprising 90.4% of the total oil composition were characterized. The main components identified were ( Z)-phytol (53.0%) and di(2-ethylhexyl)-phthalate (DEHP) (14.7%). The oil was evaluated for its in vitro antimicrobial activities against nine pathogenic microorganisms using the filter paper disc diffusion method. Moderate antimicrobial activity was observed against five of the pathogens assayed. In addition, the essential oil was tested against the sweet potato weevil, Cylas formicarius elegantulus. Strong knockdown insecticidal activity was observed.

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Composition, in vitro Anti-inflammatory, Antioxidant and Antimicrobial Activities of the Leaf Essential Oil of Machilus konishii from Taiwan

Natural Product Communications ◽

10.1177/1934578x1601100944 ◽

2016 ◽

Vol 11 (9) ◽

pp. 1934578X1601100

Author(s):

Yu-Chang Su ◽

Kuan-Ping Hsu ◽

Chen-Lung Ho

Keyword(s):

Essential Oil ◽

Antimicrobial Activities ◽

Raw 264.7 ◽

Nitric Oxide Production ◽

Leaf Oil ◽

Leaf Essential Oil ◽

Anti Inflammatory ◽

Main Components ◽

Antioxidant And Antimicrobial Activities

The chemical composition and in vitro anti-inflammatory, antioxidant and antimicrobial activities of the leaf essential oil of Machilus konishii has been investigated. The essential oil was isolated using hydrodistillation in a Clevenger-type apparatus, and characterized by GC–FID and GC–MS. Sixty-six compounds were identified, representing 100% of the oil. The main components identified were α-pinene (33.9%), β-pinene (13.9%), and thymol (12.0%). The leaf oil was able to reduce nitric oxide production by lipopolysaccharide-activated murine macrophages RAW 264.7 without reducing the cell viability. In addition, the leaf oil showed strong antioxidant and antimicrobial activities. The major ingredient of the oil that was responsible for the anti-inflammatory, antioxidant and antimicrobial activities was thymol.

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Chemical Composition and Biological Activities of the Essential Oil from Artemisia herba-alba Growing Wild in Tunisia

Natural Product Communications ◽

10.1177/1934578x1300800333 ◽

2013 ◽

Vol 8 (3) ◽

pp. 1934578X1300800 ◽

Cited By ~ 2

Author(s):

Ismail Amri ◽

Laura De Martino ◽

Aurelio Marandino ◽

Hamrouni Lamia ◽

Hanana Mohsen ◽

...

Keyword(s):

Chemical Composition ◽

Essential Oil ◽

Biological Activities ◽

Antimicrobial Activities ◽

Lepidium Sativum ◽

Mediterranean Ecosystem ◽

Bacterial Strains ◽

Phytotoxic Activity ◽

Main Components

Aromatic plants can interfere in the Mediterranean ecosystem, mainly by the introduction in the environment of volatile compounds. For this reason, we studied the chemical composition and the possible phytotoxic and antimicrobial activities of the essential oil extracted from leaves of Tunisian Artemisia herba-alba Asso. The chemical composition of the essential oil, obtained by hydrodistillation, was analyzed by GC and GC-MS. In all, 24 compounds were identified. The main components were camphor (39.1.%), chrysanthenone (15.0%) and cis-thujone (7.8%). The essential oil was evaluated for its in vitro phytotoxic activity against germination and initial radical growth of Raphanus sativus L., Lepidium sativum L., Sinapis arvensis L., Triticum durum L. and Phalaris canariensis L. seeds. The radicle elongation of the five seeds was affected to different extents by the oil, while germination was not affected. The oil, when tested against eight selected bacterial strains, showed low antimicrobial activity. The chemical composition of the oil of A. herba-alba can help in the chemosystematics of this complex genus. However, the recorded biological activities seem to be neither ecologically nor medicinally significant.

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Chemical composition and antibacterial activity of Origanum ramonense essential oil on the β-lactamase and extended- spectrum β-lactamase urinary tract isolates

Bangladesh Journal of Pharmacology ◽

10.3329/bjp.v13i3.36897 ◽

2018 ◽

Vol 13 (3) ◽

pp. 280 ◽

Cited By ~ 3

Author(s):

Haitham N. Qaralleh

Keyword(s):

Antibacterial Activity ◽

Urinary Tract ◽

Essential Oil ◽

Video Clip ◽

Enterobacter Aerogenes ◽

Diffusion Method ◽

Disc Diffusion ◽

Extended Spectrum ◽

Total Oil

The aim of this study was to evaluate the antibacterial activity of Origanum ramonense essential oil extracted from the air-dried leaves against β-lactamase and extended-spectrum β-lactamase obtained from the patients with urinary tract infection. The essential oil was extracted by hydrodistillation and analyzed by GC–MS. In vitro antibacterial activity was studied using disc diffusion and microdilution methods. Twenty compounds were identified representing 97.8% of the total oil. The major components were carvacrol (84.6%), p-cymene (4.3%) and γ-terpinene (3.3%). The oil showed a broad spectrum of antibacterial activity against all tested isolates. Staphylococcus aureus, S. epidermidis, Klebsiella pneumoniae and Enterobacter aerogenes had the lowest minimum inhibitory concentration values (0.015 µg/mL) followed by Escherichia coli (0.14 µg/mL). The lowest susceptible strains to oil were Pseudomonas aeruginosa, Proteus mirabilis, E. coli 25922 and P. aeruginosa 10145. The bacteriostatic and bactericidal effects at concentrations as low as 0.015 µg/mL indicated the potent antibacterial activity of O. ramonense. Video Clip of Methodology:Disc Diffusion method: 2 min 16 sec <a href="https://www.youtube.com/v/HUZqSngRvmQ">Full Screen</a> <a href="https://www.youtube.com/watch?v=HUZqSngRvmQ">Alternate</a>

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Antimicrobial Activities of Essential Oil and Methanol Extract ofTeucrium montanum

Evidence-based Complementary and Alternative Medicine ◽

10.1093/ecam/nem108 ◽

2007 ◽

Vol 4 (s1) ◽

pp. 17-20 ◽

Cited By ~ 37

Author(s):

Nenad Vukovic ◽

Tanja Milosevic ◽

Slobodan Sukdolak ◽

Slavica Solujic

Keyword(s):

Essential Oil ◽

Methanol Extract ◽

Antimicrobial Activities ◽

Fungal Species ◽

Diffusion Method ◽

Disc Diffusion ◽

Inhibitory Effects ◽

Disc Diffusion Method ◽

Antifungal Effect

This study was designed to examine the chemical composition of essential oil and thein vitroantimicrobial activities of essential oil and methanol extract ofTeucrium montanum. The inhibitory effects of essential oil and methanol extracts ofT. montanumwere tested against 13 bacterial and three fungal species by using disc-diffusion method. GC/MS analyses revealed that essential oil contains mainly δ-cadinene (17.19%), β-selinene (8.16%) α-calacorene (4.97%), 1,6-dimethyl-4-(1-methylethyl)-naphthalene (4.91%), caryophyllene (4.35%), copaene (4.23%), torreyol (3.91%), 4-terpineol (3.90%), cadina-1,4-diene (3.39%), β-sesquiphellandrene (3.34%), τ-cadinol (3.12%) and γ-curcumene (3.18%). The essential oil has antibacterial as well as antifungal effect.

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Biological Activity and Composition of the Essential Oil of Dracocephalum Moldavica L. Grown in Iran

Natural Product Communications ◽

10.1177/1934578x0800300930 ◽

2008 ◽

Vol 3 (9) ◽

pp. 1934578X0800300 ◽

Cited By ~ 4

Author(s):

Ali Sonboli ◽

Mehran Mojarrad ◽

Abbas Gholipour ◽

Samad Nejad Ebrahimi ◽

Mitra Arman

Keyword(s):

Staphylococcus Aureus ◽

Antimicrobial Activity ◽

Biological Activity ◽

Essential Oil ◽

Geranyl Acetate ◽

Oil Analysis ◽

Inhibition Zones ◽

Main Components ◽

Total Oil

The biological activity and composition of the essential oil of the aerial flowering parts of D. moldavica have been investigated. The oil analysis was performed by GC and GC-MS. Thirteen compounds were identified, representing 99.8% of the oil. Oxygenated monoterpenes (96.3%) were found to be the principal group of compounds, of which neral, geranial, geranyl acetate and geraniol with 32.1, 21.6, 19.9 and 17.6% of the total oil were the main constituents, respectively. The in vitro antimicrobial activity of the essential oil and its main components, citral, geraniol and geranyl acetate, showed that all of the tested microorganisms were highly inhibited by the essential oil with inhibition zones ranged from 15 – 41 mm for bacteria and 29 – 30 mm for fungi. The most sensitive microorganisms were Bacillus subtilis, Staphylococcus aureus and S. epidermidis with the lowest MIC values of 0.9 mg/mL. Considering sensitivity screening, it is conceivable that the activity of the oil from D. moldavica could be attributed mainly to the presence of citral.

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