Beyond dissolution: Xerostomia rinses affect composition and structure of biomimetic dental mineral in vitro

Xerostomia, known as dry mouth, is caused by decreased salivary flow. Treatment with lubricating oral rinses provides temporary relief of dry mouth discomfort; however, it remains unclear how their composition affects mineralized dental tissues. Therefore, the objective of this study was to analyze the effects of common components in xerostomia oral rinses on biomimetic apatite with varying carbonate contents. Carbonated apatite was synthesized and exposed to one of the following solutions for 72 hours at varying pHs: water-based, phosphorus-containing (PBS), mucin-like containing (MLC), or fluoride-containing (FC) solutions. Post-exposure results indicated that apatite mass decreased irrespective of pH and solution composition, while solution buffering was pH dependent. Raman and X-ray diffraction analysis showed that the addition of phosphorus, mucin-like molecules, and fluoride in solution decreases mineral carbonate levels and changed the lattice spacing and crystallinity of bioapatite, indicative of dissolution/recrystallization processes. The mineral recrystallized into a less-carbonated apatite in the PBS and MLC solutions, and into fluorapatite in FC. Tap water did not affect the apatite lattice structure suggesting formation of a labile carbonate surface layer on apatite. These results reveal that solution composition can have varied and complex effects on dental mineral beyond dissolution, which can have long term consequences on mineral solubility and mechanics. Therefore, clinicians should consider these factors when advising treatments for xerostomia patients.

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Effect of a newly developed pastille on the salivary flow rate in subjects with dry mouth symptoms: a randomized, controlled, monocentric clinical study

BMC Oral Health ◽

10.1186/s12903-021-01471-w ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

S. Bielfeldt ◽

D. Wilhelm ◽

C. Neumeister ◽

U. Schwantes ◽

K. -P. Wilhelm

Keyword(s):

Surface Tension ◽

Flow Rate ◽

Gum Arabic ◽

Salivary Flow ◽

Salivary Flow Rate ◽

Dry Mouth ◽

Main Ingredient ◽

Control Product ◽

Swallowing Problems ◽

The Mean

Abstract Background Xerostomia is associated with several diseases and is a side effect of certain drugs, resulting from reduced saliva secretion. Often, aged and sometimes younger people suffer from (idiopathic) xerostomia. Chewing gum and sucking pastilles may relieve symptoms of xerostomia by increasing the salivary flow rate due to the mechanical effect of sucking and gustatory stimulation. Swallowing problems and the urge to cough or experiencing a tickling sensation in the throat might be alleviated through a reduction in dry mouth symptoms. We investigated whether a pastille containing four polysaccharides increased the salivary flow rate and relieved the symptoms of dry mouth. Methods Participating subjects with xerostomia were randomized into two equally balanced treatment groups. Subjects received the pastille on Day 1 and a control product (Parafilm®) on Day 3, or vice versa. Unstimulated saliva was collected every 2.5 min for 0–10 min. Stimulated saliva was collected after subjects sucked the pastille or the control product. The salivary flow rate was determined gravimetrically, and, in parallel, the feeling of dry mouth was assessed using a visual analog scale. Saliva surface tension was measured in pooled saliva samples (0–5 min of sampling). Additionally, in stimulated saliva from six subjects who sucked the pastille, the presence of the main ingredient—gum arabic—was examined by Raman spectroscopy. Results Chewing the pastille significantly increased the mean salivary flow rate by 8.03 g/10 min compared to the mean changes after chewing the control product (+ 3.71 g/10 min; p < 0.0001). The mean score of dry mouth was significantly alleviated by the pastille (− 19.9 ± 17.9 mm) compared to the control product (− 3.3 ± 18.1 mm). No difference between the two products was seen regarding the saliva surface tension. Gum arabic was present in the saliva of all investigated subjects for up to 10 min after sucking the pastille. Conclusions The pastille was well tolerated and effective in increasing the salivary flow rate and reducing mouth dryness after sucking. These results were in line with the detection of the main ingredient, gum arabic, in saliva for up to 10 min after sucking the pastille. Trial registration German Register Clinical Trials (Deutsches Register Klinische Studien, DRKS) DRKS-ID: DRKS00017393, Registered 29 May 2019, https://www.drks.de/drks_web/navigate.do?navigationId=trial. HTML&TRIAL_ID = DRKS00017393.

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Anticholinergic Burden and Dry Mouth in Middle-Aged People

JDR Clinical & Translational Research ◽

10.1177/2380084419844511 ◽

2019 ◽

Vol 5 (1) ◽

pp. 62-70

Author(s):

A. Tiisanoja ◽

A.-M.H. Syrjälä ◽

A. Kullaa ◽

P. Ylöstalo

Keyword(s):

Regression Models ◽

Antihypertensive Drugs ◽

Salivary Flow ◽

Dry Mouth ◽

Subjective Feeling ◽

Oral Diseases ◽

Middle Aged ◽

Aged People ◽

Prophylactic Measures ◽

Anticholinergic Burden

Introduction:Anticholinergic burden refers to the cumulative effect of taking 1 or more drugs with anticholinergic properties. At the moment, little is known about the association between the anticholinergic burden and dry mouth.Objectives:The objective of this article was to study, whether an anticholinergic burden is associated with dry mouth among middle-aged people.Methods:The study population included 1,345 people aged 46 y from the Northern Finland Birth Cohort 1966 (NFBC1966) study, who took part in a clinical medical and dental examination during 2012–2013. Medication data comprised both self-reported drug use and information obtained from the national register. Anticholinergic burden was measured using 10 different anticholinergic scales. Dry mouth was defined on the basis of having either a subjective feeling of dry mouth (xerostomia) or objectively measured low unstimulated or stimulated whole salivary flow rates (hyposalivation). Poisson regression models with robust error variance were used to estimate relative risk (RR). Regression models were adjusted for sex, smoking, diabetes, rheumatoid diseases, depressive symptoms, anxiety, total number of drugs, and antihypertensive drugs.Results:Approximately 14% of the participants reported having xerostomia and about 2% had hyposalivation. The RRs of different anticholinergic scales for xerostomia varied from 1.05 to 1.68. The scales’ RRs were between 0.89 and 2.03 for low unstimulated whole salivary flow (<0.1 mL/min) and between 0.59 and 1.80 for low stimulated whole salivary flow (<0.7 mL/min). Seven of 10 studied anticholinergic scales associated statistically significantly with dry mouth, either with xerostomia or hyposalivation.Conclusion:Most of the anticholinergic scales were associated with dry mouth, either with xerostomia or hyposalivation. There was considerable variation in the strength of the associations between anticholinergic scales and dry mouth.Knowledge Transfer Statement:The findings of this study suggest that dentists should take notice of the use of drugs with anticholinergic properties and their harmful effects among middle-aged people. Dentists should provide these patients with necessary guidance on how to cope with dry mouth and give them prophylactic measures against oral diseases associated with dry mouth.

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Influence of the centrosome on the structure of nucleated microtubules.

The Journal of Cell Biology ◽

10.1083/jcb.100.4.1185 ◽

1985 ◽

Vol 100 (4) ◽

pp. 1185-1191 ◽

Cited By ~ 127

Author(s):

L Evans ◽

T Mitchison ◽

M Kirschner

Keyword(s):

Lattice Structure ◽

Neuroblastoma Cells ◽

Nucleation Sites ◽

Self Assembled ◽

Brain Microtubules

The capacity of the centrosome to influence the lattice structure of nucleated microtubules was studied in vitro. Brain microtubules self-assembled to give predominantly (98%) 14-protofilament microtubules. However, under exactly the same conditions of assembly they grew off of purified centrosomes from neuroblastoma cells to give mostly (82%) 13-protofilament microtubules. Thus, the nucleation sites on the centrosome constrained the microtubule lattice to yield the number of protofilaments usually found in vivo.

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THE UPTAKE OF TRITIATED LYSINE VASOPRESSIN BY RAT AND PIG PITUITARY NEURAL LOBES IN VITRO

Journal of Endocrinology ◽

10.1677/joe.0.0500669 ◽

1971 ◽

Vol 50 (4) ◽

pp. 669-677 ◽

Cited By ~ 4

Author(s):

B. A. EDWARDS

Keyword(s):

Tap Water ◽

Exchange Chromatography ◽

Control Group ◽

Breakdown Product ◽

Nacl Solution ◽

Water Control ◽

Neural Lobe ◽

Lysine Vasopressin ◽

And Control

SUMMARY Uptake of tritiated lysine vasopressin ([3H]LVP) was studied in halved neural lobes of rats (which had been given either tap water (control group) or 2% (w/v) NaCl solution as drinking water for 4 days) as well as in slices of pig neural lobe. Uptake of radioactivity into the neural lobes was shown but analysis of the extracts of incubated lobes of both species by ion exchange chromatography showed that very little of it remained in the tissue as hormone. In addition, some radioactivity was associated with trichloroacetic acid-insoluble proteins. After 90 min of incubation, and after correction for the breakdown, the uptake of unchanged [3H]LVP, expressed as a tissue: medium ratio, was 0·14 ± 0·04 and 0·09 ± 0·03 (mean ± s.e.m.) for the saline-treated and control rats respectively, while the tissue: medium ratios for the breakdown product(s) were 6·47 ± 0·45 and 5·50 ± 0·36. The results suggest uptake of [3H]LVP into the cell with almost complete intracellular breakdown of the hormone.

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Generation and transit pathway of H+ is critical for inhibition of palmar sweating by iontophoresis in water

Journal of Applied Physiology ◽

10.1152/jappl.1993.75.5.2258 ◽

1993 ◽

Vol 75 (5) ◽

pp. 2258-2264 ◽

Cited By ~ 48

Author(s):

K. Sato ◽

D. E. Timm ◽

F. Sato ◽

E. A. Templeton ◽

D. S. Meletiou ◽

...

Keyword(s):

Sweat Rate ◽

Tap Water ◽

Inhibitory Effect ◽

Sweat Glands ◽

Galvanic Current ◽

Silicone Grease ◽

Secretory Coil ◽

Cathodal Current ◽

Palmar Sweating

Passing galvanic current across the skin (known as "tap water iontophoresis" or TWI) inhibits sweating; however, its mechanism of action is unclear. Using improved methods, we confirmed that anodal current has more of an inhibitory effect than cathodal current, water is superior to saline, and the inhibitory effect is a function of the amperage used. To address the importance of current flowing through the pores, a layer of silicone grease was placed on the skin to reduce the shunt pathway across the epidermis. With silicone, total skin conductance decreased 60% without the sweat pores being occluded, swelling of the stratum corneum and collapse of the poral lumen was prevented, and current-induced inhibition of sweating was enhanced, most likely because of an increase in current density in the pores. The pH of anodal water, but not of saline, dropped to 3, whereas that of cathodal water increased to 10 during passage of current through the skin. Acidified anodal water was superior to alkaline water. Sweat glands isolated from TWI-induced anhidrotic palmar skin responded to methacholine in vitro, but the sweat rate and pharmacological sensitivity were slightly lowered. Thus the strong acidity generated by hydrolysis of water in the anodal bath and the further accumulation of H+ in the sweat duct by anodal current may be responsible for TWI-induced inhibition of sweating due to an unknown lesion(s) in the duct or sweat pore. The secretory coil function may also be altered because of exposure to intense acidity during TWI. The importance of H+ movement into the sweat pore for inhibition of sweating could be further exploited to develop new strategies for the control of sweating.

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A Graded Multifunctional Hybrid Scaffold with Superparamagnetic Ability for Periodontal Regeneration

International Journal of Molecular Sciences ◽

10.3390/ijms19113604 ◽

2018 ◽

Vol 19 (11) ◽

pp. 3604 ◽

Cited By ~ 8

Author(s):

Simone Sprio ◽

Elisabetta Campodoni ◽

Monica Sandri ◽

Lorenzo Preti ◽

Tobias Keppler ◽

...

Keyword(s):

Cell Fate ◽

Alveolar Bone ◽

Tape Casting ◽

Periodontal Regeneration ◽

Dental Tissue ◽

Hybrid Scaffold ◽

Dental Tissues ◽

Biomineralization Process ◽

Direct Cell

The regeneration of dental tissues is a still an unmet clinical need; in fact, no therapies have been completely successful in regenerating dental tissue complexes such as periodontium, which is also due to the lack of scaffolds that are able to guide and direct cell fate towards the reconstruction of different mineralized and non-mineralized dental tissues. In this respect, the present work develops a novel multifunctional hybrid scaffold recapitulating the different features of alveolar bone, periodontal ligament, and cementum by integrating the biomineralization process, and tape casting and electrospinning techniques. The scaffold is endowed with a superparamagnetic ability, thanks to the use of a biocompatible, bioactive superparamagnetic apatite phase, as a mineral component that is able to promote osteogenesis and to be activated by remote magnetic signals. The periodontal scaffold was obtained by engineering three different layers, recapitulating the relevant compositional and microstructural features of the target tissues, into a monolithic multifunctional graded device. Physico-chemical, morphological, and ultrastructural analyses, in association with preliminary in vitro investigations carried out with mesenchymal stem cells, confirm that the final scaffold exhibits a good mimicry of the periodontal tissue complex, with excellent cytocompatibility and cell viability, making it very promising for regenerative applications in dentistry.

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Evaluation of coconut water neutralized by different agents on the viability of human fibroblasts: an in vitro study

Revista de Odontologia da UNESP ◽

10.1590/1807-2577.09216 ◽

2016 ◽

Vol 45 (4) ◽

pp. 234-239 ◽

Cited By ~ 1

Author(s):

Priscilla Barbosa Ferreira SOARES ◽

Aletheia Moraes ROCHA ◽

Manuella Verdinelli de Paula REIS ◽

Camilla Christian Gomes MOURA ◽

Carlos José SOARES

Keyword(s):

Cell Viability ◽

Tap Water ◽

Human Fibroblasts ◽

In Vitro Study ◽

Positive Control ◽

Negative Control ◽

Coconut Water ◽

Ph Adjustment ◽

Adjustment Method

Abstract Objective This study evaluated four types of pH adjustment of the coconut water (CW) on viability of human fibroblasts (HFF). Material and method Natural and industrialized CW were adjusted to pH 7.0 using: (1) Sodium Hidroxide (NaOH), (2) Sodium bicarbonate (NaHCO3), (3) Triethanolamine (C6H15NO3), (4) 2-Amino-2-Methil-1-Propanol (C4H11NO). Fibroblasts were plated at 2×104/ well in 96 well plates and maintained in the CW solutions for 2 h and 4 h. Positive control was represented by HFF maintained in DMEM and the negative control by tap water. Cell viability was analyzed by MTT formazan method. Data were analyzed by 3-way ANOVA followed by Tukey’s and Dunnet’s test. Result There are no significant effect on the cell viability regarding type of CW, period of evaluation, and the interactions between CW and period of evaluation, CW and pH adjustment method, pH adjustment method and period of evaluation (p>0.05). Conclusion The product used for CW pH adjustment did not influenced HFF viability, thought there are a tendency of better performance in natural CW.

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Bioengineered Teeth from Cultured Rat Tooth Bud Cells

Journal of Dental Research ◽

10.1177/154405910408300703 ◽

2004 ◽

Vol 83 (7) ◽

pp. 523-528 ◽

Cited By ~ 259

Author(s):

M.T. Duailibi ◽

S.E. Duailibi ◽

C.S. Young ◽

J.D. Bartlett ◽

J.P. Vacanti ◽

...

Keyword(s):

Tissue Engineering ◽

Stem Cells ◽

Mesenchymal Stem Cells ◽

Engineering Applications ◽

Adult Rat ◽

Dental Tissues ◽

Biodegradable Scaffolds ◽

Bioengineered Tooth ◽

Tooth Tissue

The recent bioengineering of complex tooth structures from pig tooth bud tissues suggests the potential for the regeneration of mammalian dental tissues. We have improved tooth bioengineering methods by comparing the utility of cultured rat tooth bud cells obtained from three- to seven-day post-natal (dpn) rats for tooth-tissue-engineering applications. Cell-seeded biodegradable scaffolds were grown in the omenta of adult rat hosts for 12 wks, then harvested. Analyses of 12-week implant tissues demonstrated that dissociated 4-dpn rat tooth bud cells seeded for 1 hr onto PGA or PLGA scaffolds generated bioengineered tooth tissues most reliably. We conclude that tooth-tissue-engineering methods can be used to generate both pig and rat tooth tissues. Furthermore, our ability to bioengineer tooth structures from cultured tooth bud cells suggests that dental epithelial and mesenchymal stem cells can be maintained in vitro for at least 6 days.

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Phosphorylation cascade regulates the formation and maturation of rotaviral replication factories

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.1717944115 ◽

2018 ◽

Vol 115 (51) ◽

pp. E12015-E12023 ◽

Cited By ~ 14

Author(s):

Jeanette M. Criglar ◽

Ramakrishnan Anish ◽

Liya Hu ◽

Sue E. Crawford ◽

Banumathi Sankaran ◽

...

Keyword(s):

Lattice Structure ◽

Crystallographic Analysis ◽

Kinase Assay ◽

Nonstructural Proteins ◽

Cytoplasmic Inclusions ◽

Protein Levels ◽

Dual Specificity ◽

Infected Cells ◽

Phosphorylation Cascade

The rotavirus (RV) genome is replicated and packaged into virus progeny in cytoplasmic inclusions called viroplasms, which require interactions between RV nonstructural proteins NSP2 and NSP5. How viroplasms form remains unknown. We previously found two forms of NSP2 in RV-infected cells: a cytoplasmically dispersed dNSP2, which interacts with hypophosphorylated NSP5; and a viroplasm-specific vNSP2, which interacts with hyperphosphorylated NSP5. Other studies report that CK1α, a ubiquitous cellular kinase, hyperphosphorylates NSP5, but requires NSP2 for reasons that are unclear. Here we show that silencing CK1α in cells before RV infection resulted in (i) >90% decrease in RV replication, (ii) disrupted vNSP2 and NSP5 interaction, (iii) dispersion of vNSP2 throughout the cytoplasm, and (iv) reduced vNSP2 protein levels. Together, these data indicate that CK1α directly affects NSP2. Accordingly, an in vitro kinase assay showed that CK1α phosphorylates serine 313 of NSP2 and triggers NSP2 octamers to form a lattice structure as demonstrated by crystallographic analysis. Additionally, a dual-specificity autokinase activity for NSP2 was identified and confirmed by mass spectrometry. Together, our studies show that phosphorylation of NSP2 involving CK1α controls viroplasm assembly. Considering that CK1α plays a role in the replication of other RNA viruses, similar phosphorylation-dependent mechanisms may exist for other virus pathogens that require cytoplasmic virus factories for replication.

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