scholarly journals In vitro experiments of Pediculus humanus capitis (Phthiraptera: Pediculidae) resistance to permethrin and 6-paradol in East Jakarta: Detoxification enzyme activity and electron microscopic changes in lice

2021 ◽  
pp. 3065-3075
Author(s):  
Rizal Subahar ◽  
Lisawati Susanto ◽  
Rachmanin Aidilla ◽  
Annisa Putri Aulia ◽  
Yulhasri Yulhasri ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Oxidase Activity ◽  
Morphological Changes ◽  
Head Louse ◽  
Pediculus Humanus Capitis ◽  
Detoxification Enzyme ◽  
Pediculus Humanus ◽  
The Dead ◽  
Physical Changes

Background and Aim: Pediculus humanus capitis, the human head louse, remains a global health problem. This study evaluated the resistance of head lice to permethrin and 6-paradol mediated by in vitro detoxification enzyme activity experiments and to describe physical changes in the lice using scanning electron microscopy (SEM). Materials and Methods: The adult stages of P. h. capitis were collected from patients exposed to 1% permethrin and three different concentrations of 6-paradol (0.00005%, 0.0001%, and 0.00015%) using a filter paper diffusion bioassay. Healthy P. h. capitis adults served as the control. The in vitro bioassays were conducted after 10, 20, 30, and 60 min of exposure. The activities of acetylcholinesterase (AChE), glutathione S-transferase (GST), and oxidase were analyzed. Physical changes in the lice were analyzed using SEM. Results: Permethrin and 6-paradol exhibited low toxicity against the lice. At 60 min, 1% permethrin had killed 36.7% of the lice present, while 6-paradol had killed 66.7-86.7%. Permethrin induced significantly elevated AChE, GST, and oxidase activity; 6-paradol also caused significantly elevated AChE, GST, and oxidase activity. Permethrin did not cause any ultrastructural morphological changes on the lice, while 6-paradol severely damaged the head, thorax, respiratory spiracles, and abdomen of the dead lice. Conclusion: This in vitro experimental of P. h. capitis is the first study to report P. h. capitis in East Jakarta shows complete resistance to permethrin and 6-paradol, and to describe the associated increase in AChE, GST, and oxidase activity. It was observed that 6-paradol severely damaged the head, thorax, respiratory spiracles, and abdomen of the dead lice.

Identification and characterization of an esterase involved in malathion resistance in the head louse Pediculus humanus capitis

2014 ◽  
Vol 112 ◽  
pp. 13-18 ◽  
Author(s):  
Deok Ho Kwon ◽  
Ju Hyeon Kim ◽  
Young Ho Kim ◽  
Kyong Sup Yoon ◽  
J. Marshall Clark ◽  
...  
Keyword(s):  
Head Louse ◽  
Pediculus Humanus Capitis ◽  
Pediculus Humanus ◽  
Identification And Characterization

scholarly journals Essential Oils as a Potential Treatment Option for Pediculosis

Planta Medica ◽  
2020 ◽  
Vol 86 (09) ◽  
pp. 619-630 ◽  
Author(s):  
Kerdalidec Candy ◽  
Mohammad Akhoundi ◽  
Valérie Andriantsoanirina ◽  
Rémy Durand ◽  
Christiane Bruel ◽  
...  
Keyword(s):  
Essential Oils ◽  
Head Louse ◽  
Treatment Options ◽  
Residual Activity ◽  
Environmental Toxicity ◽  
Pediculus Humanus ◽  
Synthetic Chemicals ◽  
Major Factors ◽  
Ectoparasite Infestation

AbstractPediculosis is a prevalent ectoparasite infestation caused by lice. The head louse (Pediculus humanus capitis) and body louse (Pediculus humanus humanus) are obligatory parasites whose only known hosts are humans. Pediculosis is probably the most common ectoparasitic infestation, affecting up to 80% of the population in several countries, and particularly prevalent in the infant population worldwide. Several treatment options, including shampoos and creams containing insecticides, have been introduced for the treatment of pediculosis. Recently, the use of synthetic chemicals to control human lice has raised concerns pertaining to human health and the environment. Therefore, increasing efforts have been undertaken to develop effective pediculicides with low environmental toxicity and minimal environmental residual activity. In this study, we focus on the essential oils derived from 22 plant genera, their constituents, and the major factors that play important roles in the effectiveness of these oils in the treatment of pediculosis. Furthermore, we discuss the advantages and limitations of the mentioned essential oils, and ultimately suggest those demonstrating the most effective in vitro pediculicidal activities. The genera such as Aloysia, Cinnamomum, Eucalyptus, Eugenia, Lavandula, Melaleuca, Mentha, Myrcianthes, Origanum, Pimpinella, and Thymus appear to be more efficient against lice. These genera are rich in anethole, 1,8-cineole, cinnamaldehyde, p-cymene, eugenol, linalool, limonene, pulegone, terpinen-4-ol, and thymol compounds.

Ins(1,4,5)P3 3-kinase-A overexpression induces cytoskeletal reorganization via a kinase-independent mechanism

2008 ◽  
Vol 414 (3) ◽  
pp. 407-417 ◽  
Author(s):  
Sabine Windhorst ◽  
Christine Blechner ◽  
Hong-Ying Lin ◽  
Christian Elling ◽  
Marcus Nalaskowski ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Calcium Release ◽  
Morphological Changes ◽  
Three Dimensional ◽  
Culture Conditions ◽  
Enzymatic Function ◽  
Marker Proteins ◽  
Independent Mechanism ◽  
Kinase A

In the present study, effects of increased IP3K-A [Ins(1,4,5)P3 3-kinase-A] expression were analysed. H1299 cells overexpressing IP3K-A formed branching protrusions, and under three-dimensional culture conditions, they exhibited a motile fibroblast-like morphology. They lost the ability to form actin stress fibres and showed increased invasive migration in vitro. Furthermore, expression levels of the mesenchymal marker proteins vimentin and N-cadherin were increased. The enzymatic function of IP3K-A is to phosphorylate the calcium-mobilizing second messenger Ins(1,4,5)P3 to (Ins(1,3,4,5)P4. Accordingly, cells overexpressing IP3K-A showed reduced calcium release and altered concentrations of InsPs, with decreasing concentrations of Ins(1,4,5)P3, InsP6 and Ins(1,2,3,4,5)P5, and increasing concentrations of Ins(1,3,4,5)P4. However, IP3K-A-induced effects on cell morphology do not seem to be dependent on enzyme activity, since a protein devoid of enzyme activity also induced the formation of branching protrusions. Therefore we propose that the morphological changes induced by IP3K-A are mediated by non-enzymatic activities of the protein.

Pyrethroid Pediculicide Resistance of Head Lice in Canada Evaluated by Serial Invasive Signal Amplification Reaction

2010 ◽  
Vol 14 (3) ◽  
pp. 115-118 ◽  
Author(s):  
Danielle Marcoux ◽  
Kathleen G. Palma ◽  
Nalini Kaul ◽  
Hilliary Hodgdon ◽  
Andrea Van Geest ◽  
...  
Keyword(s):  
Allele Frequency ◽  
Signal Amplification ◽  
Head Louse ◽  
Human Head ◽  
The United States ◽  
Head Lice ◽  
Pediculus Humanus Capitis ◽  
Pediculus Humanus ◽  
S Allele ◽  
Amplification Reaction

Background: Most people in the United States and Canada with pediculosis will be treated with neurotoxic pediculicides containing pyrethrins or pyrethroids. Their widespread use led to significant resistance reported from various countries. Although treatment failures are frequently observed in Canada, the resistance frequency to pyrethroid pediculicide of human head lice (Pediculus humanus capitis) has not been determined. Objective: To determine the knockdown resistance ( kdr) allele frequency in human head louse populations in Canada. Methods: Patients infested with Pediculus humanus capitis, aged 4 to 65 years, residents of Ontario, Quebec, and British Columbia, were participants. Head lice were collected by combing and picking the enrolled subjects' hair. Lice were analyzed by serial invasive signal amplification reaction (SISAR) for genotyping the T917I mutation of lice indicating permethrin resistance. The permethrin-resistant kdr allele (R allele) frequency could then be evaluated in the head lice collected in Canada. Results: Of the head louse populations analyzed, 133 of 137 (97.1%) had a resistant (R) allele frequency, whereas only 4 of 137 (2.9%) had a susceptible (S) allele frequency. Conclusions: The 97.1% resistant (R) allele frequency in head lice from Canada could explain the treatment failures encountered with pyrethrin and pyrethroid pediculicide treatments in Canadian populations infested with Pediculus humanus capitis as the latter will not be eliminated by those pediculicides.

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