scholarly journals Recent applications of quantitative analytical FTIR spectroscopy in pharmaceutical, biomedical, and clinical fields: A brief review

2022 ◽  
Vol 41 (1) ◽  
pp. 21-33
Author(s):  
Khairi Mustafa Fahelelbom ◽  
Abdullah Saleh ◽  
Moawia M. A. Al-Tabakha ◽  
Akram A. Ashames
Keyword(s):  
High Performance Liquid Chromatography ◽  
Fourier Transform ◽  
Ftir Spectroscopy ◽  
High Performance ◽  
Scientific Literature ◽  
Current Status ◽  
Full Potential ◽  
Biomedical Sciences ◽  
The Subject ◽  
Shed Light

Abstract Qualitative Fourier transform infrared (FTIR) spectroscopy has long been established and implemented in a wide variety of fields including pharmaceutical, biomedical, and clinical fields. While the quantitative applications are yet to reach their full potential, this technique is flourishing. It is tempting to shed light on modern engaging and the applicability of analytical quantitative FTIR spectroscopy in the aforementioned fields. More importantly, the credibility, validity, and generality of the application will be thoroughly demonstrated by reviewing the latest published work in the scientific literature. Utilizing FTIR spectroscopy in a quantitative approach in pharmaceutical, biomedical, and interdisciplinary fields has many undeniable advantages over traditional procedures. An insightful account will be undertaken in this regard. The technique will be introduced as an appealing alternative to common methods such as high performance liquid chromatography. It is anticipated that the review will offer researchers an update of the current status and prospect on the subject among the pharmacy and biomedical sciences both in academic and industrial fields.

"Cap" sequences is poly(A)-rich RNA from dry wheat embryos

1981 ◽  
Vol 59 (10) ◽  
pp. 868-870 ◽  
Author(s):  
Byron G. Lane
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Messenger Rna ◽  
High Performance ◽  
Higher Plants ◽  
Rna Molecules ◽  
Wheat Embryos ◽  
The Subject ◽  
Widespread Interest

Although template-active RNA in dry seeds and embryos has attracted widespread interest, there have been no published reports about 5′-terminai "capping" sequences in such RNA. Boro[3H]hydride labeling of periodate-oxidized termini and high performance liquid chromatography of cap oligonucleotides have been used to compare terminal sequences in poly(A)-rich RNA from dry and germinating embryos. As is the case in germinating embryos, poly(A)-rich RNA from dry embryos contains only "type 0" cap sequences, i.e., m7G(5′)ppp(5′)N, in which m7G is the 7-methylguanosine cap and N is any of the classical ribonucleosides: adenosine (A), guanosine (G), cytidine (C), and uridine (U). Striking differences between the cell-free translational capacities of bulk messenger RNA (mRNA) populations from dry and germinating embryos are not reflected in signal differences in their proportions of "type 0" cap structures: in general, there is approximately 40% m7G(5′)ppp(5′)A, with roughly equivalent amounts of m7G(5′)ppp(5′)G and m7G(5′)ppp(5′)C accounting for most of the remaining sequences. The findings with mRNA from dry plant embryos serve to emphasize interesting differences between patterns of methylation in the capped and uncapped RNA molecules in higher plants and animals; these differences have not been previously noted in the literature and are the subject of brief comment in this paper.

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