Comparative Study of Chemical Constituents & Antioxidant Activity in Shade-Dried and Sun- Dried Fruit Peel of Punica granatum LINN

Background: The natural antioxidants have an important role in the prevention of many diseases. The aim of study is to investigate phytochemical components, antioxidant activity and acute oral toxicity of Pomegranate (Punica granatum L.) fruit peel (PFP) extract. Materials and methods: Phytochemicals of PFP were determined by qualitative chemical tests, thin layer chromatography, total polyphenol and flavonoid contents. The PFP extract was evaluated for antioxidant activity by DPPH assay and MDA assay. In vivo acute oral toxicity test was conducted using Karber-Behrens method to determine LD50. Results: Results illustrated that PFP mainly contains flavonoids, alkaloids, tannins, triterpenes, saponins, and coumarins. PFP extract exhibited the total polyphenol and flavonoid contents with 189.97 mg gallic acid equivalent/g dry weight and 9.42 mg quercetin equivalent/g dry weight, respectively. The DPPH free radical scavenging and anti-lipid peroxidation activities of PFP extract were expressed with IC50 value of 4.80 μg/mL and 0.38 μg/ mL, sequentially. Simultaneously, the Dmax (the maximum dose administered to mice that no toxicity was observed) of PFP extract was determined to be 21.28 g/kg, equivalent to 35.64 g dried herb. Conclusion: The PFP extract is relatively safe and revealed high antioxidant activity. Key words: Punica granatum L.; polyphenols; flavonoids; gallic acid; quercetin; antioxidant activity; acute oral toxicity

Download Full-text

Comparative Study on Two Variants of Laghupanchamula (A Compound Ayurvedic Formulation) for Important Groups of Phytochemicals and Antioxidant Activity

Journal of Drug Research in Ayurvedic Sciences ◽

10.5005/jp-journals-10059-0015 ◽

2017 ◽

Vol 2 (3) ◽

pp. 157-163

Author(s):

Shivani Ghildiyal ◽

Apurva Joshi ◽

Vinod K Joshi

Keyword(s):

Antioxidant Activity ◽

Comparative Study ◽

High Performance ◽

Hplc Analysis ◽

Shikimic Acid ◽

Ricinus Communis ◽

Chemical Constituents ◽

Tribulus Terrestris

ABSTRACT Introduction Laghupanchamula is a compound formulation prepared by combinations of roots of five herbs. Two variants of Laghupanchamula have been described in Ayurvedic classics where beside four common herbs fifth one is either Gokshura (Tribulus terrestris L.) or Eranda (Ricinus communis L.). The objective of the study is to make comparison between two variants of Laghupanchmula with respect to important group of phytochemicals and antioxidant activity to corroborate the science behind their therapeutic utility. Materials and methods Standard methods have been followed for quantitative determination of total quantity of phenols, tannins, flavonoids, and flavonols, and in vitro antioxidant activity in variants of Laghupanchmula formulations. Qualitative high-performance liquid chromatography (HPLC) analysis has also been performed to establish presence/absence of important chemical constituents in formulations. Results Additional quantity of phenols, tannins, flavonoids, and flavonols has been observed in Laghupanchamula variant containing Eranda than the variant containing Gokshura. Greater antioxidant activity has also been found in formulation containing Eranda. The HPLC analysis revealed the presence of shikimic acid, gallic acid, catechin in both formulations, but rutin has been found only in formulation containing Eranda. Conclusion From the results of experiments it has been observed that the formulation containing Eranda has more antioxidant activity as it contains more quantities of phenol, tannins, flavonoids, and flavonols than the formulation containing Gokshura. How to cite this article Ghildiyal S, Joshi A, Joshi VK. Comparative Study on Two Variants of Laghupanchamula (A Compound Ayurvedic Formulation) for Important Groups of Phytochemicals and Antioxidant Activity. J Drug Res Ayurvedic Sci 2017;2(3):157-163.

Download Full-text

Evaluation of antidiabetic, hypolipedimic and antioxidant activity of hydroalcoholic extract of leaves and fruit peel of Punica granatum in male Wistar albino rats

Journal of Natural Science Biology and Medicine ◽

10.4103/0976-9668.149085 ◽

2015 ◽

Vol 6 (1) ◽

pp. 56 ◽

Cited By ~ 26

Author(s):

KartikJ Salwe ◽

DevenderO Sachdev ◽

Yogesh Bahurupi ◽

Manimekalai Kumarappan

Keyword(s):

Antioxidant Activity ◽

Punica Granatum ◽

Albino Rats ◽

Fruit Peel ◽

Hydroalcoholic Extract ◽

Wistar Albino Rats

Download Full-text

COMPARATIVE STUDY ON TOTAL POLYPHENOLS, FLAVONOIDS CONTENT, AND ANTIOXIDANT ACTIVITY OF PROPOLIS FROM VIET NAM AND OTHER COUNTRIES

Science and Technology Development Journal ◽

10.32508/stdj.v14i2.1945 ◽

2011 ◽

Vol 14 (2) ◽

pp. 66-74

Author(s):

Hai Xuan Nguyen ◽

Nhan Trung Nguyen ◽

Mai Thi Thanh Nguyen

Keyword(s):

Antioxidant Activity ◽

Comparative Study ◽

Biological Activities ◽

Chemical Constituents ◽

Total Polyphenols ◽

Anti Cancer ◽

Ic50 Values ◽

Dpph Scavenging Activity ◽

Dpph Scavenging ◽

Plant Sources

Propolis is a resinous mixture that honey-bee collect from tree buds, sap flows, or other botanical sources. The chemical constituents of propolis depends on area as well as plant sources. Almost propolis contains polyphenols and flavonoids, except Myanmar propolis contains triterpenoids. Propolis is well known to various biological activities such as antibacterial, antioxidant, anti-inflammatory and anti-cancer. There is no study on chemical constituents and biological acitivities of Vietnamese propolis, therefore, we comparative study on total polyphenols, flavonoids content, and antioxidant activity of propolis from Vietnam and other countries such as Brazil, Indonesia, Mexico, Myanmar, and China. The results showed that, propolis from China, Mexico, Brazil green type and red type contained high amount of polyphenols and flavonoids, and a corresponding high antioxidant capacity. The IC50 values of DPPH scavenging activity were 33.65, 35.98, 46.59 và 55.49 μg mL-1, respectively, and IC50 values of xanthine oxidase inhibitory activity were 43.84, 6.07, 9.16 và > 100 μg mL-1, respectively. Propolis from Vietnam and Myanmar contained small amount of polyphenol and they showed less antioxidant activity.

Download Full-text