Antibacterial Activity of Polygonum pulchrum Blume Ethanol Extract  on Staphylococcus aureus and Escherichia coli

The emergence of resistant bacteria strain has become a global health concern. It encourages the exploration of potential antibacterial agents, particularly from natural sources. The aim of this study was to investigate the antibacterial activity of ethanol extract of root, stems, leaves, and flowers of Polygonum pulchrum Blume against Staphylococcus aureus and Escherichia coli, through disc diffusion method using cup-plate method. Inhibition zone against S. aureus from roots, stems, leaves, and flowers ethanol extract were 3.5 mm, 2.5 mm, 2.25 mm, and 2.62 mm, respectively, while the inhibition zone against E. coli were 2.25 mm, 2.12 mm, 1.62 mm, and 1.75 mm, respectively. In conclusion, ethanol extract of root, stem, leaves, and flower of P. pulchrum Bl possessed weak antibacterial activity against S. aureus and E. coli.Keywords: P. pulchrum Bl, antibacterial, E. coli, S. aureus, cup-plate technique

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UJI AKTIVITAS ANTIBAKTERI EKSTRAK DAUN TEMBELEKAN (Lantana camara Linn) TERHADAP PERTUMBUHAN Staphylococcus aureus dan Escherichia coli

Biocelebes ◽

10.22487/bioceb.v15i2.15540 ◽

2022 ◽

Vol 15 (2) ◽

pp. 90-97

Author(s):

Gaby Maulida Nurdin

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Ethanol Extract ◽

Lantana Camara ◽

Diffusion Method ◽

E Coli ◽

Activity Test ◽

Growth Inhibitory ◽

One Way Anova

This study aimed to determine the effect of concentration ethanol extract from tembelakan leaf (Lantana camara Linn) on bacteria growth of Staphylococcus aureus and Escherichia coli. Extraction was done by maceration using ethanol 96% and then separated using rotary evaporator. Antibacterial activity test of the ethanol extract by Well agar diffusion method. Variation in crude extract saponin used in this study was 5%, 10%, 15%, 20%, 25% and positive controls were used for comparison with Amoxicilin and Chloramphenicole concentration of 25 µg/mL and DMSO as a negative control. The results of antibacterial activity test is indicated by the formation of growth inhibitory region S. aureus and E. coli. The result of growth inhibitory regions was analyzed by One way ANOVA. One way ANOVA test results indicate that there are effects of ethanol extract concentration of tembelekan leaf (L. camara Linn) against S. aureus and E. coli. Effective concentration of ethanol extract tembelekan leaf (L. camara Linn) when compared with positive control to inhibit the growth of S. aureus and E. coli is at 25% with a relatively strong antibacterial activity. Test with phytochemicals screening method which is showed that tembelekan leaf contains the flavanoid, saponins, and tannins compounds as antibacterial

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UJI AKTIVITAS ANTIBAKTERI DAUN JERUK KALAMANSI (Citrofortunella microcarpa) TERHADAP BAKTERI Staphylococcus aureus DAN Escherichia coli

Alotrop ◽

10.33369/atp.v3i2.10122 ◽

2019 ◽

Vol 3 (2) ◽

Author(s):

Deza Oktasila ◽

Nurhamidah Nurhamidah ◽

Dewi Handayani

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Essential Oil ◽

Ethanol Extract ◽

Inhibition Zone ◽

Raw Material ◽

Diffusion Method ◽

Citrus Leaves ◽

Escherichia Coli Bacteria

This study aims to examine the antibacterial activity of ethanol extract and essential oil of Kalamansi citrus leaves (Citrofortunella microcarpa) against Staphylococcus aureus and Escherichia coli bacteria. The raw material of Kalamansi citrus leaves is obtained from the Village of Pondok Kubang, Bengkulu Tengah (3.70060S, 102.35780E). Ethanol extract from Kalamansi citrus leaves was obtained by maceration using ethanol 96%, then ethanol extract was made dilution concentration 40 ; 20; , 10; and 5%. The essential oil of Kalamansi citrus leaves is obtained by water-vapor distillation, then made variations of concentration 20;, 15;, 10; and 5%. The method used to test the antibacterial activity is the paper disc diffusion method , the antibacterial activity is shown by the diameter of the inhibiting zone formed. The data of antibacterial test result were analyzed by using One Way Anova test which showed the effect of treatment on the growth of test bacteria seen from the value (P <0,01) and continued by Duncan test to know the effect of the treatment. The results showed that ethanol extract of Kalamansi citrus leaves had antibacterial activity against S. aureus and E. coli bacteria with moderate inhibitory diameter is 7.20 and 5.73 mm at concentration 40%, while antibacterial activity on essential oil of Kalamansi citrus leaves is categorized as strong with inhibition zone diameter is 14.83 and 13.00 mm at concentration 20%.

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Relationship Among Antibiotic Residues And Antibacterial Activity Of The Endemic Spurge Honey (Euphorbia Resinifera O. Berg) From Morocco

Emirates Journal of Food and Agriculture ◽

10.9755/ejfa.2020.v32.i11.2190 ◽

2020 ◽

pp. 795

Author(s):

Rania Benjamaa ◽

Abdelkarim Moujanni ◽

Anass Terrab ◽

Rabiaa Eddoha ◽

Maryam Benbachir ◽

...

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Inhibition Zone ◽

Health Concern ◽

Resistant Bacteria ◽

Antibiotic Residues ◽

Antibiotic Resistant ◽

Liquid Chromatography Tandem Mass

Antibiotic-resistant bacteria continue to be of major health concern worldwide. In recent years, several reports and scientific articles claim the contamination of honey by antibiotics, detectable concentrations of antibiotic residues in honey are illegal. They, may cause hypersensitivity or resistance to drug therapy in humans, and are perceived by consumers as undesirable. In this sense, the purpose of this work was to examine the antibacterial activity of the Euphorbia resinifera (E. resinifera) honey against Escherichia coli and Staphylococcus aureus in vitro using the well-agar diffusion assay followed by dilution range to obtain more precise minimum inhibitory concentration values. The second aim is to evaluate the presence of antibiotics in honey using a screening test: Evidence InvestigatorTM, an immuno-enzymatic method for detection of 27 antibiotic residues followed by a liquid chromatography-tandem mass spectrometry (LC-MS/MS) for confirmation of suspect samples; in order to assess the relationship between the presence of antibiotic residues and the antibacterial activity of honey. In this study, a total of 37 E. resinifera honey samples were analyzed. The results show that all samples of honey inhibited the growth of bacteria at the dilutions at 50% (v/v); the highest inhibition zone (25.98 ± 0.11 mm) was recorded from sample 5 for Staphylococcus aureus and (13.84 ± 1.10 mm) in sample 17 for Escherichia coli and that 50% (v/v) dilutions showed significant antibacterial effect compared to other dilutions (6.25, 12.5, 25% (v/v)). In all samples, there were no antibiotic residues detected except for one showing the detection of Trimethoprim at 6.48 µg kg-1. Our research is one of the first studies that relate the he relationship between the presence of antibiotic residues and the antibacterial activity of Euphorbia resinifera honey and showed that the antibacterial activity of honey might be due to the high osmotic nature, a low pH, its content of phenolic compounds and hydrogen peroxide and also to its content of methylglyoxal.

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DAYA HAMBAT EKSTRAK ETANOL Schismatoglottis sp. TERHADAP BAKTERI Staphylococcus aureus dan Escherichia coli

JURNAL ILMIAH SAINS ◽

10.35799/jis.15.2.2015.10231 ◽

2015 ◽

Vol 17 (1) ◽

pp. 149

Author(s):

Fahri Bian ◽

Febby E.F. Kandou ◽

Marhaenus J. Rumondor

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Ethanol Extract ◽

Inhibition Zone ◽

Diffusion Method ◽

Disk Diffusion Method ◽

Inhibition Zone Diameter ◽

E Coli ◽

Zone Diameter ◽

Paper Disk

DAYA HAMBAT EKSTRAK ETANOL Schismatoglottis sp. TERHADAP BAKTERI Staphylococcus aureus dan Escherichia coliABSTRAK Penelitian ini bertujuan untuk mengetahui daya hambat ekstrak etanol daun Schismatoglottis sp. terhadap bakteri Staphylococcus aureus dan Escherichia coli. Daya hambat ekstrak etanol Schismatoglottis sp. terhadap bakteri menggunakan metode Kirby-Bauer, yaitu metode difusi dengan cakram kertas. Ekstrak etanol Schismatoglottis sp. menghambat pertumbuhan S. aureus pada konsentrasi 60% dan 90% dengan diameter zona hambat 16,10 mm dan 31,33 mm serta terhadap E. coli pada konsentrasi 30%, 60%, dan 90% dengan diameter zona hambat berturut-turut 11,72 mm, 16,38 mm, dan 29,27 mm. Berdasarkan hasil penelitian, dapat disimpulkan bahwa ekstrak etanol Schismatoglottis sp. terhadap S. aureus pada konsentrasi 60% dan 90% masing-masing memiliki kekautan antibakteri termasuk kategori kuat dan sangat kuat, sedangkan terhadap E. coli pada konsentrasi 30% dan 60%, memiliki kekuatan antibakteri kategoti kuat sedangkan pada konsentrasi 90% memiliki kekuatan antibakteri kategori sangat kuat. Kata kunci : Schismatoglottis sp, Antibakteri, Staphylococcus aureus, Escherichia coli THE INHIBITION OF ETHANOL EXTRACT OF Schismatoglottis sp. AGAINST BACTERIA Staphylococcus aureus AND Escherichia coli. ABSTRACT This study aimed to determine the inhibition of ethanol extract of leaves Schismatoglottis sp. against S. aureus and E. coli. Inhibition of the ethanol extract of Schismatoglottis sp. against to bacterial using the Kirby-Bauer method, is the paper disk diffusion method. Schismatoglottis sp. ethanol extract inhibit the growth of S. aureus at a concentration of 60% and 90% inhibition zone with a diameter of 16.10 mm and 31.33 mm and against E. coli at a concentration of 30%, 60%, and 90% with inhibition zone diameter respectively 11, 72 mm, 16.38 mm and 29.27 mm. Based on the results, it can be concluded that the ethanol extract Schismatoglottis sp. against S. aureus at concentrations of 60% and 90% respectively have antibacterial kekautan categorized as strong and very strong, while against E. coli at a concentration of 30% and 60%, have a strong kategoti antibacterial strength while at a concentration of 90% has antibacterial strength very strong category. Keywords: Schismatoglottis sp, antibacterials, Staphylococcus aureus, Escherichia coli.

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Potensi Antibakteri Ekstrak Bunga Soka (Ixora coccinea L) terhadap Staphylococcus aureus dan Escherichia coli

AcTion Aceh Nutrition Journal ◽

10.30867/action.v1i2.23 ◽

2016 ◽

Vol 1 (2) ◽

pp. 130

Author(s):

Munira Munira ◽

Riska Maisarah ◽

Muhammad Nasir

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Ethanol Extract ◽

Average Diameter ◽

Inhibition Zone ◽

Diffusion Method ◽

Test Results ◽

E Coli ◽

Ethanol Extracts ◽

Ixora Coccinea

Soka plants (Ixora coccineal L.) often used to treat diarrhea, dysentery and wounds. This study was conducted to determine the effect of soka flower ethanol extract in inhibiting the growth of Staphylococcus aureus and Escherichia coli. This study is experimentally using completely randomized design (CRD) with 4 treatments and 6 repetitions which is distilled water as a control, the ethanol extract of soka flowers concentration are 100%, 75%, and 50%. Microbiological test using the diffusion method of Kirby-Baueur. Phytochemical test results soka ethanol extracts of flowers contain alkaloids, flavonoids, tannins, saponins, and triterpenoid. ANOVA test results ethanol extracts of soka flowers greatly affect the growth of S. aureus and E. coli (P=0.000). Duncan test results an average diameter of inhibition zone for the ethanol extract of soka flowers against S. aureus at a concentration of 100% (14.50 mm) was significantly different from the concentration of 75% (10.33 mm) and concentration of 50% (10.67 mm), while the average diameter of inhibition zone for the ethanol extract of soka flowers against E. coli at 100% (11.00 mm) was not significantly different from the 75% (7.83 mm) and 50% (7.50 mm). The ability of ethanol extract of soka flower in inhibition zone against S. aureus greater than E. coli. Keywords: Ixora coccinea L., inhibition zone, Staphylococcus aureus and Escherichia coli.

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Antibacterial Activity Test of the C-4-methoxyphenylcalix[4]resorcinarene Compound Modified by Hexadecyltrimethylammonium-Bromide against Staphylococcus aureus and Escherichia coli Bacteria

JKPK (Jurnal Kimia dan Pendidikan Kimia) ◽

10.20961/jkpk.v3i3.22742 ◽

2018 ◽

Vol 3 (3) ◽

pp. 201 ◽

Cited By ~ 1

Author(s):

Suryadi Budi Utomo ◽

Mita Fujiyanti ◽

Warih Puji Lestari ◽

Sri Mulyani

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Inhibition Zone ◽

Positive Control ◽

Negative Control ◽

Diffusion Method ◽

Hexadecyltrimethylammonium Bromide ◽

E Coli ◽

Escherichia Coli Bacteria

This study aims to determine the antibacterial activity of C-4-methoxyphenylcalix-[4]resorcinarene modified by Hexadecyltrimethylammonium-Bromide (Resorcinarene-HDTMA-Br) against Staphylococcus aureus and Escherichia coli bacteria. The study was conducted by testing the inhibition zone diameter with paper disc diffusion method. Variations in the concentration of the sample used were 10%; 15%; 20%; 25%; and 30% b/v. In this study, Dimethyl sulfoxide (DMSO) (sampel solvent) was used as a negative control and Chloramphenicol as a positive control. It was found that Resorsinarene-HDTMA-Br had higher antibacterial activity than the unmodified C-4-methoxyphenylcalix[4]resorcinarene (CMFKR) against the S. aureus bacteria. Both CMFKR and Resorcinarene-HDTMA-Br are not active against E. coli.

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Antibacterial Activity of Rhamnus prinoides extracts against Staphylococcus aureus and Escherichia coli

10.21203/rs.2.17220/v2 ◽

2019 ◽

Author(s):

KINDU GETA

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Infectious Diseases ◽

Pathogenic Bacteria ◽

Ethanol Extract ◽

Inhibition Zone ◽

Diffusion Method ◽

Active Components ◽

Microbial Infections

Abstract Background: Infectious diseases caused by bacteria have a large impact on public health. Increasing in resistant microbial infections intensified the search for new, safer, and more efficacious agents to combat serious microbial infections. Plants have played a central part in combating many diseases in human and domestic animal in many local communities, including Africa. Rhamnus prinoides is Ethiopian medicinal plants that have been used traditionally for the treatment of diverse infectious diseases. Therefore, this research was carried out to evaluate antibacterial activity Rhamnus prinoides extracts against pathogenic bacteria. Methods: The antimicrobial activity tests were carried out by the agar well diffusion method. Broth dilution and streak plate methods were used to determine MIC and MBC of extracts respectively. Results: The results revealed that ethanol extracts of fruits showed the highest antibacterial activity against standard strains of S.aureus with mean inhibition zone of 26±0.58, 28.33±1.2 and 33.33±0.9mm at 25, 50 and 100mg/ml respectively. The lowest mean MIC value (1.04 mg/ml) was recorded with ethanol extract of fruits against standard strains of S. aureus and the lowest mean MBC value (2.08 mg/ml) was recorded with ethanol extract of fruits against standard strains and clinical isolates of S. aureu. Conclusion: On the basis of the current findings, Rhamnus prinoides could be a good candidate in the search for new antibacterial agents from natural products against bacterial pathogens. Therefore, further studies are needed to study their toxicology and isolate the bio- active components from this plant. Key words: Antibacterial activity, Escherichia coli, MBC, MIC, Rhamnus prinoides, Staphylococcus aureus

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Aktivitas Antibakteri Minyak Atsiri Daun Tembakau terhadap Pertumbuhan Bakteri Escherichia coli dan Staphylococcus aureus

Jurnal Kefarmasian Indonesia ◽

10.22435/jki.v9i1.1219 ◽

2019 ◽

pp. 48-56 ◽

Cited By ~ 1

Author(s):

Elda Nurnasari ◽

Kristiana Sri Wijayanti

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Essential Oil ◽

Inhibition Zone ◽

Positive Control ◽

Raw Material ◽

Diffusion Method ◽

Tobacco Leaves ◽

E Coli

Tobacco plants are widely used as raw material for cigarettes, but the results of the study show that in tobacco leaves contain compounds that can be used in the pharmaceutical and health fields. Tobacco leaves contain essential oils that have a distinctive aroma. The study aims to examine the antibacterial activity of tobacco essential oil from six sources tobacco such as Temanggung, Yogyakarta, Purwodadi, Boyolali, Blitar and Probolinggo. Tobacco essential oil is obtained by the steam-water distillation method. Antibacterial activity evaluation was carried out by diffusion method and the minimum inhibitory concentration on two bacteria, Escherichia coli and Staphylococcus aureus. The yield of tobacco essential oil ranged from 0.54-3.39%. Tobacco essential oil had antibacterial activity against bacteria E. coli and S. aureus in the presence of inhibition zones in bacterial media. The biggest inhibition zone was in Yogyakarta tobacco essential oil of 26 mm for S. aureus bacteria, while for E. coli bacteria the biggest inhibition zone was Blitar tobacco essential oil which was equal to 21 mm. The inhibition zone was not much different from the inhibition zone in tetracycline antibiotic testing (positive control) which was 25 mm in S. aureus and 21.5 mm bacteria in E. coli bacteria. Tobacco essential oil from Probolinggo was able to inhibit the growth of S. aureus bacteria up to a concentration of 6.25% and E. coli bacteria to a concentration of 12.5%.

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Antibacterial Activity Test of the Ethanol in Leaves Extract of Senduduk (Melastoma malabathricum L.) Against Escherichia coli and Staphylococcus aureus

JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS ◽

10.36987/jpbn.v6i2.1766 ◽

2020 ◽

Vol 6 (2) ◽

pp. 139-152

Author(s):

Alfi Sapitri ◽

Nofita Lara ◽

Panal Sitorus

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Ethanol Extract ◽

Inhibition Zone ◽

Wild Plants ◽

Diffusion Method ◽

Inhibitory Zone ◽

Melastoma Malabathricum ◽

And Staphylococcus Aureus

Senduduk (Melastoma malabathricum L.) is one of the wild plants used as traditional medicine. Senduduk leaves are used as a medicine for sprue, boils, diarrhea and smallpox. The purpose of this study was to determine the antibacterial activity of the ethanol extract of senduduk leaves of the against Escherichia coli and Staphylococcus aureus. Senduduk leaves extracted by maceration using 96% ethanol solvent. Antibacterial activity testing is carried out using the diffusion method using paper discs.This experiment was repeated three times using concentrations of 20%, 40%, 60% and 80%.Ethanol extract of the senduduk leaves contains flavonoids, saponins, tannins and steroids/triterpenoids. The test results showed that the ethanol extract of the leaves has antibacterial activity against Escherichia coli and Staphylococcus aureus which is characterized by the inhibition zone formed around the disc paper. In Escherichia coli bacteria the smallest inhibition zone at a concentration of 20% with a diameter of inhibition zone 11 mm and the largest diameter of the inhibitory zone at a concentration of 80% with a diameter of inhibition zone 19 mm. whereas for the smallest inhibitory zone Staphylococcus aureus bacteria at a concentration of 20% with an inhibition zone diameter of 12.6 mm and the largest inhibitory zone dameter at a concentration of 80% with a diameter of the inhibitory zone 21,3 mm.The results of the study concluded that the ethanol extract of Senduduk leaves has antibacterial activity against Escherichia coli and Staphylococcus aureus.

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ANTIBACTERIAL ACTIVITY TEST OF EXTRACT ETHANOL OF JACKFRUIT LEAVES (Artocarpus heterophyllus. Lamk.) OF BACTERIA Staphylococcus aureus, Escherichia coli, Staphylococcus epidermidis and Salmonella typhi

Journal of Pharmaceutical And Sciences ◽

10.36490/journal-jps.com.v2i2.28 ◽

2020 ◽

Vol 2 (2) ◽

pp. 49-54

Author(s):

Kasta Gurning ◽

Dameria Siahaan ◽

Iksen Iksen

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Staphylococcus Epidermidis ◽

Ethanol Extract ◽

Inhibition Zone ◽

Salmonella Typhi ◽

Diffusion Method ◽

Disk Diffusion Method ◽

Artocarpus Heterophyllus

Jackfruit leaves (Artocarpus heterophyllus Lamk.) have health benefits as an antimicrobial. The leaves contain flavonoids, tannins, saponins which act as antimicrobials. The purpose of this study was to study the potential antibacterial activity of ethanol extract of jackfruit leaves (Artocarpus heterophyllus Lamk.) On the bacteria Staphylococcus aureus, Escherichia coli, Staphylococcus epidermidis, and Salmonella typhi. This research conducted with an experimental method that included the collection and processing of samples, the examination of the simplicia characterization and phytochemical screening. The concentration of jackfruit leaf ethanol extract. Used was at a concentration of 500 mg mL, 400 mg/mL, 300 mg/mL, 200 mg/mL, 100 mg/mL, 100 mg/mL, 50 mg/mL , 25 mg/mL, 10 mg/mL 30 mg/ml chloramphenicol and blanks. Using the disk diffusion method to measure the clear zone against the bacteria Staphylococcus aureus, Escherichia coli, Staphylococcus epidermidis, and Salmonella typhi. Antimicrobial inhibition of ethanol extract of jackfruit leaves against Staphylococcus aureus bacteria at a concentration of 500 mg/mL has a strong antibacterial inhibition with a diameter of 10.8 mm. The bacteria Escherichia coli, Staphylococcus epidermidis, and Salmonella typhi at a concentration of 500 mg/mL have inhibitory power, 9.2 mm, 9.6 mm, and 8.8 mm in the medium category. Positive control chloramphenicol has powerful antibacterial inhibition with an inhibition zone diameter of 28.6 mm.

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