scholarly journals Seed priming with hydrogen peroxide alleviates the effects of drought stress in rice (Oryza sativa L.) seedlings

2020 ◽  
Vol 48 (1) ◽  
pp. 273-283
Author(s):  
Weeraphorn JIRA-ANUNKUL ◽  
Wattana PATTANAGUL
Keyword(s):  
Hydrogen Peroxide ◽  
Oryza Sativa ◽  
Drought Stress ◽  
Antioxidant Enzyme ◽  
Enzyme Activities ◽  
Oryza Sativa L ◽  
Seed Priming ◽  
Rice Seedling ◽  
Antioxidant Enzyme Activities ◽  
Guaiacol Peroxidase

Drought stress is a major factor limiting crop growth and yield. Hydrogen peroxide (H2O2) is known as a signalling molecule in the plant cell in which activates multiple physiological changes that play essential roles in tolerance mechanism. This study investigated the effects of seed priming with H2O2 on growth, some physiological characteristics and antioxidant enzyme activities in rice seedling under drought stress. Rice (Oryza sativa L.) cv. Khao Dawk Mali 105 seeds were primed with 0 (distilled water), 1, 5, 10, and 15 mM H2O2 and grown for 21 days. The seedlings were subjected to drought stress by withholding water for 7 days. The results showed that priming with low concentrations of H2O2 improved plant growth and biomass as well as relative water content, malondialdehyde content, electrolyte leakage. Priming with H2O2, however, had no beneficial effect on chlorophyll content, proline and leaf total soluble sugar. Seed priming with appropriate levels of H2O2 also enhanced antioxidant enzyme activities including superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), and guaiacol peroxidase (GPX). It is concluded that seed priming with 2-10 mM H2O2, is beneficial for enhancing drought tolerance in rice seedling by increasing antioxidant capacity, which in turn reduces oxidative stress and damages to the cellular components.

scholarly journals Effects of hydrogen peroxide application on agronomic traits of rice (Oryza sativa L.) under drought stress

2021 ◽  
Vol 67 (No. 4) ◽  
pp. 221-229
Author(s):  
Weeraphorn Jira-anunkul ◽  
Wattana Pattanagul
Keyword(s):  
Hydrogen Peroxide ◽  
Oryza Sativa ◽  
Drought Stress ◽  
Yield Loss ◽  
Agronomic Traits ◽  
Foliar Application ◽  
Oryza Sativa L ◽  
Seed Priming ◽  
Grain Weight ◽  
Signal Molecule

Drought stress is a major environmental factor limiting crop growth and productivity. Hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) plays an essential role during stress response by acting as a signal molecule that activates multiple stress tolerance mechanisms. In this study, the effects of H<sub>2</sub>O<sub>2</sub> on agronomic traits were studied in rice (Oryza sativa L.) cv. Khao Dawk Mali 105 (KDML 105) was subjected to drought stress. H<sub>2</sub>O<sub>2</sub> was applied by either seed priming or foliar application method with a concentration of 1, 5, and 15 mmol/L. The results showed that both seed priming and foliar application with H<sub>2</sub>O<sub>2</sub> improved some yield components. The tiller numbers, number of panicles, number of filled grains, filled grain weight, and harvest index were improved approximately 1.13, 1.04, 1.23, 1.21, and 1.1 times compared to the untreated plants. Foliar application, however, helps the plant by reducing yield loss as indicated by a 0.5-time reduction in the number of unfilled grain and lower unfilled grain weight. It was suggested that 5 mmol/L H<sub>2</sub>O<sub>2</sub> was the most effective concentration to alleviate the effect of drought stress during the reproductive stage in rice.

scholarly journals Concomitant  accumulations of ions, osmoprotectants and antioxidant system-related  substances provide salt tolerance capability to succulent extreme-halophyte Scorzonera hieraciifolia             

2021 ◽  
Author(s):  
Cansu ALTUNTAŞ ◽  
Rabiye TERZİ
Keyword(s):  
Hydrogen Peroxide ◽  
Salt Tolerance ◽  
Antioxidant Enzyme ◽  
Antioxidant System ◽  
Enzyme Activities ◽  
Inorganic Ions ◽  
Antioxidant Enzyme Activities ◽  
Guaiacol Peroxidase ◽  
Tolerance Mechanism ◽  
Calcium Magnesium

Halophytes adapting to live in salinized areas can activate some tolerance mechanism through signal compounds to cope with salinity. However, the role of co-activity of signal compounds in salt tolerance of halophytes is not yet fully understood. We have firstly detected that Scorzonera hieraciifolia with fleshy shoots is a succulent extreme-halophyte and researched the changes in signal compounds involved in the salt tolerance mechanism, including inorganic ions, osmoprotectants and substances related to antioxidant system. The levels of signal compounds such as calcium, magnesium, proline, soluble sugar, hydrogen peroxide, superoxide, ascorbate and glutathione concomitantly increased when thickness of shoot tissues enhanced under excess salinity. There were 3.3-fold, 5-fold, 8-fold and 10-fold enhancements in the levels of inorganic ions (Ca and Mg), hydrogen peroxide, ascorbate and glutathione in the shoots treated with excess salinity, respectively. Contents of sodium, potassium and chlorine, and antioxidant enzyme activities, superoxide dismutase, guaiacol peroxidase, ascorbate peroxidase, catalase and glutathione reductase, also increased in the salinized shoots. Western blot analysis showed that the increases in antioxidant enzyme activities were consistent with increases in their protein contents. The results suggest that extraordinary salt tolerance capacity in Scorzonera hieraciifolia, a succulent extreme-halophyte can be improved by modulated accumulations of signal compounds, especially calcium, magnesium, osmoprotectants, reactive oxygen species and antioxidant substances. Moreover, massive induction of antioxidant enzymes can make strong contributions to salt stress tolerance of S. hieraciifolia.

Cadmium-induced changes in antioxidant enzyme activities in rice (Oryza sativa L. cv. Dongjin)

2002 ◽  
Vol 45 (3) ◽  
pp. 134-140 ◽  
Author(s):  
Mohammad Babar Ali ◽  
Hyun Sik Chun ◽  
Byung Ki Kim ◽  
Chin Bum Lee
Keyword(s):  
Oryza Sativa ◽  
Antioxidant Enzyme ◽  
Enzyme Activities ◽  
Oryza Sativa L ◽  
Antioxidant Enzyme Activities ◽  
Induced Changes

scholarly journals Drought Stress Responses of Sunflower Germplasm Developed after Wide Hybridization

2016 ◽  
Vol 4 (10) ◽  
pp. 859
Author(s):  
Roumiana Dimova Vassilevska-Ivanova ◽  
Lydia Shtereva ◽  
Ira Stancheva ◽  
Maria Geneva
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Drought Stress ◽  
Antioxidant Enzyme ◽  
Ascorbate Peroxidase ◽  
Enzyme Activities ◽  
Antioxidant Enzyme Activities ◽  
Guaiacol Peroxidase ◽  
Fresh Weight ◽  
Breeding Lines

Response of sunflower germplasms viz. cultivated sunflower H. annuus and two breeding lines H. annuus x T. rotundifolia and H. annuus x V. encelioides developed after wide hybridization were used for identification of drought tolerant sunflower genotypes at the seedling growth stage. Three water stress levels of zero (control), -0.4, and -0.8 MPa were developed using polyethyleneglycol-6000 (PEG-6000). Physiological and biochemical stress determining parameters such as root and shoots length, fresh weight, antioxidant enzyme activities (superoxide dismutase (SOD), catalase (CAT), guaiacol peroxidase (GPO), ascorbate peroxidase (APX) and antioxidant metabolite content (total antioxidant capacity, total phenols and total flavonoids content) were compared between seedlings of all three genotypes. Results revealed that sunflower genotypes have similar responses at two osmotic potentials for shoot and root length and fresh weight. The data also showed that drought stresss could induce oxidative stress, as indicated by the increase level of ascorbate peroxidase and guaiacol peroxidase at -04 MPa in H. annuus cv 1114. Although the activity of ascorbate peroxidase and guaiacol peroxidase was differentially influenced by drought, the changes of antioxidant enzyme activities such as catalase, superoxide dismutase, guaiacol peroxidase, and ascorbate peroxidase subjected to drought stress follow a similar pattern in both breeding lines, indicating that similar defense systems might be involved in the oxidative stress injury in sunflowers. Increase in content of phenols and flavonoids were detected for all three genotypes under stress, which showed that these were major antioxidant metabolites in scavenging cellular H2O2.

scholarly journals Study of silicon effects on antioxidant enzyme activities and osmotic adjustment of wheat under drought stress

2011 ◽  
Vol 47 (No. 1) ◽  
pp. 17-27 ◽  
Author(s):  
S. Tale Ahmad ◽  
R. Haddad
Keyword(s):  
Drought Stress ◽  
Antioxidant Enzyme ◽  
Osmotic Adjustment ◽  
Enzyme Activities ◽  
Soluble Protein ◽  
Membrane Damage ◽  
Triticum Aestivum L ◽  
Growth Stages ◽  
Antioxidant Enzyme Activities ◽  
Randomized Design

The effect of silicon (Si) was investigated on the major antioxidant enzyme activities including superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), peroxidase (POD), relative water content (RWC), chlorophyll and soluble protein contents, proline (Pro) and glycine betaine (GB) accumulation in three different growth stages (2<sup>nd</sup>, 4<sup>th</sup> leaf and tillering stages) of wheat (Triticum aestivum L.) plants under drought stress. The experiment was performed in a completely randomized design for three treatments including control, drought and Si-drought (2mM silicate sodium/kg) with three replications in a greenhouse. The results indicated that Si partially offset the negative impacts of drought stress increasing the tolerance of wheat by rising Pro and GB accumulation and soluble protein content. Compared with the plants treated with drought, applied Si significantly enhanced the activities of SOD, CAT, APX and POD. In contrast, drought stress caused a considerable decrease in RWC, chlorophyll and soluble protein contents. This Si effect was time-dependent and became stronger in the tillering stage. The results of the present experiment coincided with the conclusion that Si alleviates water deficit of wheat by preventing the oxidative membrane damage and may be associated with plant osmotic adjustment.

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