Identification of locally isolated entomopathogenic Fusarium species from the soil of Changa Manga Forest, Pakistan and evaluation of their larvicidal efficacy against Aedes aegypti

Abstract Dengue fever vectored by the mosquito Aedes aegypti is one of the most rapidly spreading insect-borne diseases. Current reliance of dengue vector control is mostly on chemical insecticides. Growing insecticide resistance in the primary mosquito vector, Aedes aegypti, limits the effectiveness of vector control through chemical insecticides. These chemical insecticides also have negative environmental impacts on animals, plants and human health. Myco-biocontrol agents are naturally occurring organisms and are found to be less damaging to the environment as compared to chemical insecticides. In the present study, entomopathogenic potential of local strains of fungi isolated from soil was assessed for the control of dengue vector. Local fungal isolates presents better alternative to introducing a foreign biocontrol strain, as they may be better adapted to environmental conditions of the area to survive and may have more entomopathogenic efficacy against target organism. Larvicidal efficacy of Fusarium equiseti and Fusarium proliferatum was evaluated against Aedes aegypti. Local strains of F. equiseti (MK371718) and F. proliferatum (MK371715) were isolated from the soil of Changa Manga Forest, Pakistan by using insect bait method. Larvicidal activity of two Fusarium spp. was tested against forth instar larvae of A. aegypti in the laboratory, using concentrations 105, 106, 107 and 108 conidia /ml. LC50 values for F. equiseti after 24h, 48h, 72h and 96h of exposure were recorded as 3.8x 108, 2.9x107, 2.0x107, and 7.1x106 conidia /ml respectively while LC50 values for F. proliferatum were recorded as 1.21x108, 9.6x107, 4.2x107, 2.6x107 conidia /ml respectively after 24h, 48h, 72h and 96h of exposure. The results indicate that among two fungal strains F. equiseti was found to be more effective in terms of its larvicidal activity than F. proliferatum against larvae of A. aegypti.

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Larvicidal Effect of Vinca Fruit Extract (Vinca rosea) Against Aedes aegypti Larvae and Secondary Metabolites Profile by Thin Layer Chromatography

Biology Medicine & Natural Product Chemistry ◽

10.14421/biomedich.2014.31.31-33 ◽

2014 ◽

Vol 3 (1) ◽

pp. 31

Author(s):

Rahmawati Ekaputri ◽

Sudarsono Sudarsono ◽

Budi Mulyaningsih

Keyword(s):

Aedes Aegypti ◽

Thin Layer ◽

Thin Layer Chromatography ◽

Larvicidal Activity ◽

Larval Mortality ◽

Probit Analysis ◽

Mosquito Vector ◽

Fruit Extract ◽

Vinca Rosea ◽

Layer Chromatography

Background: Vinca rosea is known contain alkaloids, it was usually used to treat various diseases. Alkaloids from Vinca leaves are also already known have larvicidal activity. Based on this toxicological activity, the fruit of Vinca rosea was selected to investigation its larvicidal activity against the 3rd instar larvae of the mosquito vector of dengue haemorrhagic fever (DHF) Aedes aegypti. Five concentrations of Vinca fruit extract were tested against the 3rd instar Aedes aegypti larvae. The different larval mortality percentages were recorded after 24 hours. Lethal concentration (LC50 anf LC90) of Vinca fruit extract were calculated using Probit analysis. Phytochemical compounds of ethanolic extract also investigated using Thin layer Chromatography (TLC). LC50 and LC90 values of fruit extract were 2.987 mg/ml and 32.861 mg/ml. Alkaloids were detected in extract.

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Co-occurrence of kdr Mutations V1016I and F1534C and Its Association With Phenotypic Resistance to Pyrethroids in Aedes aegypti (Diptera: Culicidae) Populations From Costa Rica

Journal of Medical Entomology ◽

10.1093/jme/tjz241 ◽

2019 ◽

Vol 57 (3) ◽

pp. 830-836

Author(s):

Aryana Zardkoohi ◽

David Castañeda ◽

Juan C Lol ◽

Carmen Castillo ◽

Francisco Lopez ◽

...

Keyword(s):

Costa Rica ◽

Aedes Aegypti ◽

Insecticide Resistance ◽

Vector Control ◽

Strong Association ◽

Resistance Management ◽

Management Plan ◽

Costa Rican ◽

Mosquito Vector ◽

Phenotypic Resistance

Abstract Aedes aegypti (Linnaeus, 1762) is considered the most important mosquito vector species for several arboviruses (e.g., dengue, chikungunya, Zika) in Costa Rica. The primary strategy for the control and prevention of Aedes-borne diseases relies on insecticide-based vector control. However, the emergence of insecticide resistance in the mosquito populations presents a significant threat to these prevention actions. The characterization of the mechanisms driving the insecticide resistance in Ae. aegypti is vital for decision making in vector control programs. Therefore, we analyzed the voltage-gated sodium channel (VGSC) gene for the presence of the V1016I and F1534C kdr mutations in Ae. aegypti populations from Puntarenas and Limon provinces, Costa Rica. The CDC bottle bioassays showed that both Costa Rican Ae. aegypti populations were resistant to permethrin and deltamethrin. In the case of kdr genotyping, results revealed the co-occurrence of V1016I and F1534C mutations in permethrin and deltamethrin-resistant populations, as well as the fixation of the 1534C allele. A strong association between these mutations and permethrin and deltamethrin resistance was found in Puntarenas. Limon did not show this association; however, our results indicate that the Limon population analyzed is not under the same selective pressure as Puntarenas for the VGSC gene. Therefore, our findings make an urgent call to expand the knowledge about the insecticide resistance status and mechanisms in the Costa Rican populations of Ae. aegypti, which must be a priority to develop an effective resistance management plan.

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The development of predictive tools for pre-emptive dengue vector control: a study of Aedes aegypti abundance and meteorological variables in North Queensland, Australia

Tropical Medicine & International Health ◽

10.1111/j.1365-3156.2010.02592.x ◽

2010 ◽

Vol 15 (10) ◽

pp. 1190-1197 ◽

Cited By ~ 51

Author(s):

Aishah H. Azil ◽

Sharron A. Long ◽

Scott A. Ritchie ◽

Craig R. Williams

Keyword(s):

Aedes Aegypti ◽

Vector Control ◽

Meteorological Variables ◽

Dengue Vector ◽

Predictive Tools ◽

Dengue Vector Control

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Ecological Links Between Water Storage Behaviors and Aedes aegypti Production: Implications for Dengue Vector Control in Variable Climates

EcoHealth ◽

10.1007/s10393-010-0301-6 ◽

2010 ◽

Vol 7 (1) ◽

pp. 78-90 ◽

Cited By ~ 46

Author(s):

H. Padmanabha ◽

E. Soto ◽

M. Mosquera ◽

C. C. Lord ◽

L. P. Lounibos

Keyword(s):

Aedes Aegypti ◽

Vector Control ◽

Water Storage ◽

Dengue Vector ◽

Dengue Vector Control

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AKTIVITAS EKSTRAK RIMPANG TEMULAWAK (Curcuma xanthorrhiza Roxb.) SEBAGAI LARVASIDA TERHADAP Aedes aegypti DI KECAMATAN KELAPA LIMA KOTA KUPANG

JURNAL KAJIAN VETERINER ◽

10.35508/jkv.v8i1.2282 ◽

2020 ◽

Vol 8 (1) ◽

pp. 54-68

Author(s):

Deswandi W. S. Berri ◽

Julianty Almet ◽

Diana Agustiani Wuri

Keyword(s):

Aedes Aegypti ◽

Vector Control ◽

Research Method ◽

Hemorrhagic Fever ◽

Positive Control ◽

Negative Control ◽

Control Group ◽

Mosquito Vector ◽

Curcuma Xanthorrhiza ◽

Mosquito Vector Control

Dengue hemorrhagic fever (DHF) is a disease that is found in some tropical and subtropical regions. This disease is caused by dengue virus and is transmitted to humans through the bite of the Aedes aegypti mosquito. the solution taken in controlling DHF is to break the life cycle of the Aedes aegypti mosquito. Vector control is generally carried out using synthetic larvicides, namely abate / temefos, but the use of abate can cause residues, environmental pollution, poisoning and resistance of the eradicated vectors so that natural larvasides from plants are needed namely temulawak rhizome (Curcuma xanthorrhiza Roxb.) for vector control. The purpose of this study was to determine whether temulawak rhizome extract was effective or not in killing Aedes aegypti larvae. This research method includes larva collection, identification and maintenance of mosquitoes, determining sample size, making extracts and testing effectiveness. This study used a control and experiment group with 3 repetitions in the minutes to 15, 30, 45, 60 and 1440 (24 Hours). The control group was positive control using abate and negative control using aquades while the eksperiment group used extract of temulawak rhizome (Curcuma xanthorrhiza Roxb.) with concentrations of 0.6%, 0.8%, 1%, 1.2% and 1.5%. The results of this study indicate that the temulawak rhizome extract (Curcuma xanthorrhiza Roxb.) effective as larvicides because at the lowest concentration 0.6% can kill 100% Aedes aegypti larvae.

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Co-occurrence of kdr mutations V1016I and F1534C in pyrethroid-resistant Aedes aegypti (Diptera: Culicidae) populations from Costa Rica

10.1101/704767 ◽

2019 ◽

Author(s):

Aryana Zardkoohi ◽

David Castañeda ◽

Carmen Castillo ◽

Juan C Lol ◽

Francisco Lopez ◽

...

Keyword(s):

Costa Rica ◽

Aedes Aegypti ◽

Insecticide Resistance ◽

Vector Control ◽

Resistance Management ◽

Management Plan ◽

Costa Rican ◽

Mosquito Vector ◽

Control Programs ◽

Control And Prevention

AbstractAedes aegypti (Linnaeus, 1762) is considered the most important mosquito vector species for several arboviruses (e.g., dengue, chikungunya, Zika) in Costa Rica. The main strategy for the control and prevention of Aedes-borne diseases relies on insecticide-based vector control. However, the emergence of insecticide resistance in the mosquito populations present a big threat for the prevention actions. The characterization of the mechanisms driving the insecticide resistance in Ae. aegypti are vital for decision making in vector control programs. Therefore, we analyzed the voltage-gated sodium channel gene for the presence of the V1016I and F1534C kdr mutations in pyrethroid-resistant Ae. aegypti populations from Puntarenas and Limon provinces, Costa Rica. The CDC bottle bioassays showed that both Costa Rican Ae. aegypti populations were resistant to permethrin and deltamethrin. In the case of kdr genotyping, results revealed the co-occurrence of V1016I and F1534C mutations in permethrin and deltamethrin-resistant populations, as well as the fixation of the 1534C allele. Therefore, our findings make an urgent call to expand the knowledge about the insecticide resistance status and mechanisms in the Costa Rican populations of Ae. aegypti which must be a priority to develop an effective resistance management plan.

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Larvicidal activity of different plant parts of Asparagus setaceus against dengue vector Aedes aegypti

International Journal of Mosquito Research ◽

10.22271/23487941.2021.v8.i5a.554 ◽

2021 ◽

Vol 8 (5) ◽

pp. 01-06

Author(s):

Sumanta Chakraborty ◽

Aniket Singh ◽

Goutam Chandra

Keyword(s):

Aedes Aegypti ◽

Larvicidal Activity ◽

Dengue Vector ◽

Plant Parts

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Transcriptomic analysis and molecular docking reveal genes involved in the response of Aedes aegypti larvae to an essential oil extracted from Eucalyptus

PLoS Neglected Tropical Diseases ◽

10.1371/journal.pntd.0009587 ◽

2021 ◽

Vol 15 (7) ◽

pp. e0009587

Author(s):

Ivana Sierra ◽

Jose Manuel Latorre-Estivalis ◽

Lucila Traverso ◽

Paula V. Gonzalez ◽

Ariel Aptekmann ◽

...

Keyword(s):

Molecular Docking ◽

Essential Oil ◽

Aedes Aegypti ◽

Vector Control ◽

Gene Families ◽

Control Group ◽

Mosquito Vector ◽

Rna Seq ◽

Chemosensory Proteins ◽

The Impact

Background Aedes aegypti (L.) is an urban mosquito, vector of several arboviruses that cause severe diseases in hundreds of million people each year. The resistance to synthetic insecticides developed by Ae. aegypti populations worldwide has contributed to failures in vector control campaigns, increasing the impact of arbovirus diseases. In this context, plant-derived essential oils with larvicidal activity could be an attractive alternative for vector control. However, the mode of action and the detoxificant response of mosquitoes to plant derived compounds have not been established, impairing the optimization of their use. Methods and findings Here we compare gene expression in Ae. aegypti larvae after 14 hrs of exposure to Eucalyptus camaldulensis essential oil with a control group exposed to vehicle (acetone) for the same lapse, by using RNA-Seq. We found differentially expressed genes encoding for cuticle proteins, fatty-acid synthesis, membrane transporters and detoxificant related gene families (i.e. heat shock proteins, cytochromes P450, glutathione transferases, UDP-glycosyltransferases and ABC transporters). Finally, our RNA-Seq and molecular docking results provide evidence pointing to a central involvement of chemosensory proteins in the detoxificant response in mosquitoes. Conclusions and significance Our work contributes to the understanding of the physiological response of Ae. aegypti larvae to an intoxication with a natural toxic distilled from Eucalyptus leafs. The results suggest an involvement of most of the gene families associated to detoxification of xenobiotics in insects. Noteworthy, this work provides important information regarding the implication of chemosensory proteins in the detoxification of a natural larvicide. Understanding the mode of detoxification of Eucalyptus distilled compounds could contribute to their implementation as a tool in mosquito control.

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Insecticide resistance in dengue vectors from hotspots in Selangor, Malaysia

PLoS Neglected Tropical Diseases ◽

10.1371/journal.pntd.0009205 ◽

2021 ◽

Vol 15 (3) ◽

pp. e0009205

Author(s):

Rosilawati Rasli ◽

Yoon Ling Cheong ◽

M. Khairuddin Che Ibrahim ◽

Siti Futri Farahininajua Fikri ◽

Rusydi Najmuddin Norzali ◽

...

Keyword(s):

Aedes Aegypti ◽

Insecticide Resistance ◽

Vector Control ◽

Aedes Albopictus ◽

World Health ◽

Dengue Vector ◽

Organophosphate Insecticide ◽

Potential Threat ◽

Pyrethroid Insecticides ◽

Dengue Vector Control

Background In Malaysia, dengue remains a top priority disease and usage of insecticides is the main method for dengue vector control. Limited baseline insecticide resistance data in dengue hotspots has prompted us to conduct this study. The present study reports the use of a map on the insecticide susceptibility status of Aedes aegypti and Aedes albopictus to provide a quick visualization and overview of the distribution of insecticide resistance. Method and results The insecticide resistance status of Aedes populations collected from 24 dengue hotspot areas from the period of December 2018 until June 2019 was proactively monitored using the World Health Organization standard protocol for adult and larval susceptibility testing was conducted, together with elucidation of the mechanisms involved in observed resistance. For resistance monitoring, susceptibility to three adulticides (permethrin, deltamethrin, and malathion) was tested, as well as susceptibility to the larvicide, temephos. Data showed significant resistance to both deltamethrin and permethrin (pyrethroid insecticides), and to malathion (organophosphate insecticide) in all sampled Aedes aegypti populations, while variable resistance patterns were found in the sampled Aedes albopictus populations. Temephos resistance was observed when larvae were tested using the diagnostic dosage of 0.012mg/L but not at the operational dosage of 1mg/L for both species. Conclusion The present study highlights evidence of a potential threat to the effectiveness of insecticides currently used in dengue vector control, and the urgent requirement for insecticide resistance management to be integrated into the National Dengue Control Program.

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