Wholemount immunolabeling of mouse gut tissue v1

2021 ◽  
Author(s):  
Marthe Howard ◽  
Andrea Kalinoski
Keyword(s):  
Light Cycle ◽  
Male And Female ◽  
Female Mice ◽  
Reporter Mice ◽  
Food Standard ◽  
Specific Manner ◽  
Experimental Protocols ◽  
Ad Libitum ◽  
Animal Care

Wholemount Immunolabeling-Gut The application was developed for large pieces of mouse gut tissue. Animal care, breeding procedures, and experimental protocols were approved by the UTHSC animal care and use committee. Animals were housed in an AAALAC-approved facility with a 12-hour light cycle with food (standard chow) and water ad libitum. Male and female mice aged 3 to 9 months were used in the reported studies. All reporter mice, except where indicated, were generated by crossing either R26REYFP/EGFP mice or RCL-tdTomato mice with one of the Cre lines which express the reporter in a promoter-specific manner following Cre-mediated recombination. When applicable mice were purchased from the Jackson Laboratories, Bar Harbor, Maine. All genotyping was done using primers recommended by the Jackson Laboratories according to their protocols.

Lead in the Water Supply Alters Swimming-Maze Behavior in Adult Mice

1982 ◽  
Vol 55 (2) ◽  
pp. 507-512 ◽  
Author(s):  
K. Mc Lean ◽  
G. H. Parker ◽  
M. A. Persinger
Keyword(s):  
Drinking Water ◽  
Water Supply ◽  
Tap Water ◽  
Test Block ◽  
Food Regime ◽  
Male And Female ◽  
Female Mice ◽  
Adult Mice ◽  
Body Weights ◽  
Ad Libitum

After about two weeks of exposure to either 20 ppm or approximately 2000 ppm of lead in the drinking water or tap water only and under an ad libitum or restricted food regime, albino male and female mice ( N = 48) were tested for three consecutive days (3 blocks of 3 trials per day) in a swimming maze. Body weights were not altered by lead treatments significantly. The mice treated with the lead displayed longer escape latencies and more errors than the controls on tap water. Statistically significant interactions of lead treatment by test day by test block were also apparent.

scholarly journals Sex-specific regulation of weight and puberty by the Lin28/let-7 axis

2015 ◽  
Vol 228 (3) ◽  
pp. 179-191 ◽  
Author(s):  
Christina Corre ◽  
Gen Shinoda ◽  
Hao Zhu ◽  
Diana L Cousminer ◽  
Christine Crossman ◽  
...  
Keyword(s):  
Late Onset ◽  
Pubertal Timing ◽  
Association Studies ◽  
Growth Patterns ◽  
Age At Menarche ◽  
Genome Wide Association Studies ◽  
Loss Of Function ◽  
Male And Female ◽  
Female Mice ◽  
Specific Manner

Growth and pubertal timing differ in boys and girls. Variants in/nearLIN28Bassociate with age at menarche (AAM) in genome-wide association studies and some AAM-related variants associate with growth in a sex-specific manner. Sex-specific growth patterns in response toLin28bperturbation have been detected in mice, and overexpression ofLin28ahas been shown to alter pubertal timing in female mice. To investigate further howLin28aandLin28baffect growth and puberty in both males and females, we evaluatedLin28bloss-of-function (LOF) mice andLin28again-of-function (GOF) mice. Because bothLin28aandLin28bcan act via the conserved microRNAlet-7, we also examinedlet-7GOF mice. As reported previously,Lin28bLOF led to lighter body weights only in male mice whileLin28aGOF yielded heavier mice of both sexes.Let-7GOF mice weighed less than controls, and males were more affected than females. Timing of puberty was assessed by vaginal opening (VO) and preputial separation (PS). MaleLin28bLOF and malelet-7GOF, but not female, mice displayed alteration of pubertal timing, with later PS than controls. In contrast, both male and femaleLin28aGOF mice displayed late onset of puberty. Together, these data point toward a complex system of regulation byLin28a,Lin28b, andlet-7, in whichLin28bandlet-7can impact both puberty and growth in a sex-specific manner, raising the possibility that this pathway may contribute to differential regulation of male and female growth and puberty in humans.

A subchronic oral toxicity study on medical herb extract, KIOM CRC#BP10A, in male and female mice

Planta Medica ◽  
2015 ◽  
Vol 81 (16) ◽  
Author(s):  
ES Cho ◽  
YJ Lee ◽  
JS Park ◽  
J Kim ◽  
NS Kim ◽  
...  
Keyword(s):  
Toxicity Study ◽  
Oral Toxicity ◽  
Male And Female ◽  
Female Mice ◽  
Subchronic Oral Toxicity Study ◽  
Herb Extract

Early Life Exposure to Antibiotic Alters Energy Balance during Chronic High-Fat Feeding in Adult Male and Female Mice

Diabetes ◽  
2018 ◽  
Vol 67 (Supplement 1) ◽  
pp. 1999-P ◽  
Author(s):  
HYE LIM NOH ◽  
SUJIN SUK ◽  
RANDALL H. FRIEDLINE ◽  
KUNIKAZU INASHIMA ◽  
DUY A. TRAN ◽  
...  
Keyword(s):  
Energy Balance ◽  
Adult Male ◽  
Early Life ◽  
High Fat ◽  
Male And Female ◽  
Female Mice ◽  
Early Life Exposure ◽  
Fat Feeding

Comparison of Antinociceptive Effects Induced by Kappa Opioid Agonists in Male and Female Mice

Analgesia ◽  
1999 ◽  
Vol 4 (3) ◽  
pp. 397-404 ◽  
Author(s):  
Corinne A. Patrick ◽  
M. C. Holden Ko ◽  
James H. Woods
Keyword(s):  
Kappa Opioid ◽  
Male And Female ◽  
Female Mice ◽  
Opioid Agonists ◽  
Antinociceptive Effects

Maternal separation affects the electrophysiological properties of Aδ‐fibres and nociceptive behaviours in male and female mice

2020 ◽  
Vol 80 (6) ◽  
pp. 538-546
Author(s):  
Nancy Paniagua ◽  
Rocío Girón ◽  
Carlos Goicoechea ◽  
Mª Isabel Martín‐Fontelles ◽  
Ana Bagues
Keyword(s):  
Maternal Separation ◽  
Male And Female ◽  
Electrophysiological Properties ◽  
Female Mice

scholarly journals Digging behavior discrimination test to probe burrowing and exploratory digging in male and female mice

2021 ◽  
Author(s):  
Heather L. Pond ◽  
Abigail T. Heller ◽  
Brian M. Gural ◽  
Olivia P. McKissick ◽  
Molly K. Wilkinson ◽  
...  
Keyword(s):  
Discrimination Test ◽  
Male And Female ◽  
Female Mice ◽  
Digging Behavior

scholarly journals Dissociating Representations of Time and Number in Reinforcement-Rate Learning by Deletion of the GluA1 AMPA Receptor Subunit in Mice

2021 ◽  
Vol 32 (2) ◽  
pp. 204-217
Author(s):  
Joseph M. Austen ◽  
Corran Pickering ◽  
Rolf Sprengel ◽  
David J. Sanderson
Keyword(s):  
Associative Strength ◽  
Ampa Receptor ◽  
Reinforcement Rate ◽  
Statistical Properties ◽  
Temporal Information ◽  
Receptor Subunit ◽  
Male And Female ◽  
Female Mice ◽  
Ampa Receptor Subunit ◽  
Numeric Information

Theories of learning differ in whether they assume that learning reflects the strength of an association between memories or symbolic encoding of the statistical properties of events. We provide novel evidence for symbolic encoding of informational variables by demonstrating that sensitivity to time and number in learning is dissociable. Whereas responding in normal mice was dependent on reinforcement rate, responding in mice that lacked the GluA1 AMPA receptor subunit was insensitive to reinforcement rate and, instead, dependent on the number of times a cue had been paired with reinforcement. This suggests that GluA1 is necessary for weighting numeric information by temporal information in order to calculate reinforcement rate. Sample sizes per genotype varied between seven and 23 across six experiments and consisted of both male and female mice. The results provide evidence for explicit encoding of variables by animals rather than implicit encoding via variations in associative strength.

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