Clinical course of neurological disorders in experimental hemorrhagic stroke after treatment with interleukin-2 (ronkoleukinum)

Aim. To study the influence of recombinant interleukin-2 (Ronkoleukinum) on indicators of oxidative protein modification and severity of neurologic signs in rats with experimental hemorrhagic stroke. Methods. Oxidative protein modification (by aldehyde and carboxyl products), the survival rate, neurological deficit by McGrow Stroke-index and animal psychophysiological status were studied on the model of intracerebral hemorrhage in rats after treatment with 0.01 mg/kg of interleukin-2 (Ronkoleukinum). Results. The progression of experimental hemorrhagic stroke in rats was accompanied with typical pathophysiological signs - oxidative protein modification with following neurological and cognitive disorders, followed by death of experimental animals. Administration of interleukin-2 (Ronkoleukinum) 0.01 mg/kg hampered the processes of free radical proteins damage, therefore decreasing the mortality rate in animals with intracerebral hemorrhage. Animals that were administered interleukin-2 (Ronkoleukinum) died only during the first 24 hours after the stroke, with mortality rate significantly lower compared to controls starting form the 4th day of the experiment (р 0.05). The use of interleukin-2 (Ronkoleukinum) also statistically significantly decreased the severity of post-ischemic behavioral, neurological and cognitive disorders, improving the movement activity, psychoneurological status assessed by McGrow scale, stabilizing the memory and passive avoidance reflex recovery. Conclusion: interleukin-2 (Ronkoleukinum) can effectively prevent the formation of post-stroke neuronal disorders, so its use as a nosotropic nootropic agent seems to have a good perspective.

Download Full-text

INTRACEREBRAL HEMORRHAGE (ICH) INCIDENCE PROFILE IN JEMURSARI ISLAMIC HOSPITAL DURING THE PERIOD 2017-2019

Oceana Biomedicina Journal ◽

10.30649/obj.v5i1.62 ◽

2022 ◽

Vol 5 (1) ◽

pp. 41-48

Author(s):

Hidayatullah Hidayatullah ◽

Shobihatus Syifak ◽

Choirotussanijjah Choirotussanijjah

Keyword(s):

Mortality Rate ◽

Intracerebral Hemorrhage ◽

Medical Records ◽

Hemorrhagic Stroke ◽

Epidemiological Studies ◽

Brain Parenchyma ◽

Type B ◽

Medical Record Data ◽

Record Data ◽

Male Patients

Background: Intracerebral hemorrhage or ICH or hemorrhagic stroke is caused by bleeding within brain parenchyma. Riskesdas reported that stroke patients in Indonesia experienced an increase from 7 permil in 2013 to 10.9 permil in 2018. Mortality rate for ICH is estimated 40% in 1 month and 54% in 1 year. Rumah sakit Islam Jemursari (RSI) is the only type B hospital in Wonocolo sub-district, Surabaya city. This study aimed to analyze the pattern of incidents and variations of ICH at RSI Jemursari Surabaya.Method: This was a descriptive observational study. Medical record data is collected from 2017-2019. The data were obtained from medical records section of total number of ICH, gender, age and outcome of patients. Furthermore, data is analyzed and illustrated through a bar chart and the frequency of mortality is calculated.Results: Total ICH patients at Jemursari Hospital were 310 with 192 male patients and 118 female patients over 3 years. Meanwhile, the most groups experienced ICH were 45-64 years, followed by +65 age group. This is consistent with several epidemiological studies related to ICH, where the incidence of ICH increases with increasing age. The mortality rate for ICH patients, in the 2017-2019 periode, was around 23-30%.Conclusion: It can be concluded that male more susceptible to ICH than female subjects. Meanwhile, the mortality rate for ICH patients ranged from 23-30% in the 2017-2019 period. It is necessary to carry out further evaluation related to other data from the patient. So it could describe incidence rate as well as an overview of the ICH profile at RSI Jemursari.

Download Full-text

Relation of Volume of Hemorrhage With Mortality Within First 48 Hours in Hemorrhagic Stroke

KYAMC Journal ◽

10.3329/kyamcj.v12i3.56553 ◽

2021 ◽

Vol 12 (3) ◽

pp. 127-132

Author(s):

Sk Abdullah Al Mamun ◽

Saiyeedur Rahman ◽

Sayedur Rahman Sheikh ◽

Abdul Wadud ◽

Gobindo Gain

Keyword(s):

Mortality Rate ◽

Glasgow Coma Scale ◽

Intracerebral Hemorrhage ◽

Hemorrhagic Stroke ◽

Mortality Rates ◽

Advanced Age ◽

Coma Scale ◽

Level Of Consciousness ◽

Hemorrhage Volume ◽

Scale 8

Background: Hemorrhagic stroke accounts for 10-15% of all strokes with higher mortality rates than cerebral infarction. Intracerebral hemorrhage has a reported 30-day mortality of 44% to 51%, with almost half of the death occurs within the first 48 hours. Advanced age, low level of consciousness, large volume of hemorrhage has been linked with poor outcome. Objectives: To predict early outcome of hemorrhagic stroke patient in relation with age, Glasgow Coma Scale, volume of hemorrhage and ventricular extension. Materials and Methods: Hospital based prospective study carried out in hundred hemorrhagic stroke patients. The formula of ABC/2 was used to calculate hemorrhage volume in bedside by using CT scan. Results: 1st month mortality rates of hemorrhagic stroke was 44% with 45.45% of patients died within the first 48 hours of onset. Mean age of patients of hemorrhagic stroke was 61.2 ± 13.88 years. Mortality rate of intracerebral hemorrhage after age of 60 was 51.06% in 1st month. Volume of intracerebral hemorrhage was the strongest predictor of both 48 hours and 30 days mortality. Using three categories of intracerebral hemorrhage (X for < 30 ml, Y for 30 - 50 ml and Z for > 50 ml group) calculated by ABC/2 formula showed 100% mortality rate in Z group, 50% in Y group and only 12% mortality rate in X group in 1st month. Among all death, 61.5% of Z group 25% of Y group and 16.67% of patients of X group died within 48 hours. Two categories of Glasgow Coma Scale (≤ 8 and ≥ 9) were used and shown death rates 80.77% in GCS ≤ 8 and 4.55% in GCS ≥ 9 in 1st month. Conclusion: Volume of intracerebral hemorrhage in combination with advanced age, initial Glasgow Coma Scale is a powerful and easy to use in both 48 hours and 1st month mortality in patients with spontaneous intracerebral hemorrhage. KYAMC Journal. 2021;12(3): 127-132

Download Full-text

Differential central nervous system responses folloqing single and multiple recombinant interleukin-2 infusions

Journal of Neuroimmunology ◽

10.1016/0165-5728(90)90018-i ◽

1990 ◽

Vol 28 (3) ◽

pp. 249-260 ◽

Cited By ~ 36

Author(s):

Mary D. Ellison ◽

Richard J. Krieg ◽

John T. Povlishock

Keyword(s):

Central Nervous System ◽

Nervous System ◽

Interleukin 2 ◽

Recombinant Interleukin 2

Download Full-text

Low molecular weight B-cell growth factor and recombinant interleukin-2 are together able to generate cytotoxic T lymphocytes with LAK activity from the bone marrow cells of children with acute lymphoblastic leukemia

Blood ◽

10.1182/blood.v74.4.1355.1355 ◽

1989 ◽

Vol 74 (4) ◽

pp. 1355-1359 ◽

Cited By ~ 1

Author(s):

MX Zhou ◽

HW Jr Findley ◽

AH Ragab

Keyword(s):

Bone Marrow ◽

Acute Lymphoblastic Leukemia ◽

Growth Factor ◽

Cytotoxic T Lymphocytes ◽

Lymphoblastic Leukemia ◽

Interleukin 2 ◽

Leukemic Cells ◽

Recombinant Interleukin 2 ◽

B Cell Growth Factor ◽

Lak Activity

Abstract We are reporting here that low-mol wt B-cell growth factor (LMW-BCGF) and recombinant interleukin-2 (rIL-2) are together able to induce CD3+ cytotoxic T lymphocytes (CTL) with lymphokine-activated killer cell (LAK) activity from the bone marrow (BM) cells of children with acute lymphoblastic leukemia (ALL). Ficoll-Hypaque (FH)-separated BM cells were obtained from patients with active disease (at diagnosis N = 13, in relapse N = 15) and in complete remission (CR; N = 12). CD3+ cells were removed by Leu-4 antibody and immunobeads. Cells were cultured (10(5) cells/mL) in semisolid media with rIL-2 (100 mu/mL), LMW-BCGF (0.1 mu/mL), and the combination of rIL-2 plus LMW-BCGF, respectively, for seven to ten days. Pooled colonies were harvested for phenotyping. LMW-BCGF plus rIL-2 induced large numbers of CD3+ colonies from CD3- precursors. rIL-2 alone did not induce colony formation. In addition, cells were cultured in liquid media with LMW-BCGF, rIL-2, and the combination of LMW-BCGF plus rIL-2, respectively, for seven to 21 days. They were harvested for phenotyping, and cytotoxicity assays were performed v K562, Raji, and autologous leukemic cells. LMW-BCGF plus rIL-2 induced significant expansion of CD3+ cells from CD3- precursors, and these cells were activated to kill autologous leukemic cells in addition to Raji and K562 cell lines. LMW-BCGF or rIL-2 alone did not induce significant expansion or activation of cytotoxic CD3- cells. Our hypothesis is that LMW-BCGF plus rIL-2 stimulates the proliferation and activation of CD3- precursors from the BM cells of children with acute leukemia to become CD3+ cells that have LAK activity. This finding may have therapeutic implications.

Download Full-text