Comparison of the antibacterial activity against Escherichia coli of silver nanoparticle produced by chemical synthesis with biosynthesis

The synthesis of silver nanoparticles (Ag NPs) has been carried out using different methods, mainly by biological and chemical methods; however, comparing antibacterial activity of Ag NPs synthesized by these methods has not been conducted before. In this study, silver nanoparticles (Ag NPs) were synthesized by methods using reducing agent NaBH4/carboxymethyl cellulose (CMC) and fungal strain Trichoderma asperellum (T.asperellum). The formation of silver nanoparticles was observed visually by color change and identified by Ultraviolet-visible (UV – vis) spectroscopy. The transmission electron microscopy (TEM) image illustrated almost nanoparticles with spherical shape and average diameter of 4.1 ± 0.2 nm and 2.1 ± 0.2 nm of samples produced from chemical reduction and biosynthesis respectively. Both samples after 180 days storing have been separated lightly, but the agglomeration and absorbance peak shifting were not observed which proved the high stability of synthesized Ag NPs. Antimicrobial activity against human bacterial pathogen Escherichia coli (E. coli) showed that the inhibition zone produced by “biosynthesis” and “chemical reduction” Ag NPs were 3.17 cm and 2.42 cm respectively. With nanoparticles size smaller than 2 mm, antibacterial activity of “biosynthesis” Ag NPs against E. coli was 31 % higher than “chemical reduction” Ag NPs, although the concentration of Ag NPs produced by biosynthesis was about 10-fold less.

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Antimicrobial Activity of Silver Nanoparticles Prepared Under an Ultrasonic Field

International Journal of Pharmaceutical Sciences and Nanotechnology ◽

10.37285/ijpsn.2011.4.3.8 ◽

2011 ◽

Vol 4 (3) ◽

pp. 1491-1496

Author(s):

Umadevi M ◽

Rani T ◽

Balakrishnan T ◽

Ramanibai R

Keyword(s):

Escherichia Coli ◽

Antimicrobial Activity ◽

Silver Nanoparticles ◽

Reduction Method ◽

Therapeutic Potential ◽

Chemical Reduction ◽

Ultrasonic Field ◽

Great Promise ◽

Chemical Reduction Method ◽

E Coli

Nanotechnology has great promise for improving the therapeutic potential of medicinal molecules and related agents. In this study, silver nanoparticles of different sizes were synthesized in an ultrasonic field using the chemical reduction method with sodium borohydride as a reducing agent. The size effect of silver nanoparticles on antimicrobial activity were tested against the microorganisms Staphylococcus aureus (MTCC No. 96), Bacillus subtilis (MTCC No. 441), Streptococcus mutans (MTCC No. 497), Escherichia coli (MTCC No. 739) and Pseudomonas aeruginosa (MTCC No. 1934). The results shows that B. subtilis, and E. coli were more sensitive to silver nanoparticles and its size, indicating the superior antimicrobial efficacy of silver nanoparticles.

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Enhancement of the Antibacterial Efficiency of Silver Nanoparticles against Gram-Positive and Gram-Negative Bacteria Using Blue Laser Light

International Journal of Photoenergy ◽

10.1155/2019/2528490 ◽

2019 ◽

Vol 2019 ◽

pp. 1-12 ◽

Cited By ~ 11

Author(s):

Anes Al-Sharqi ◽

Kasing Apun ◽

Micky Vincent ◽

Devagi Kanakaraju ◽

Lesley Maurice Bilung

Keyword(s):

Silver Nanoparticles ◽

Antibacterial Activity ◽

Exposure Time ◽

Cell Membranes ◽

Laser Light ◽

Blue Laser ◽

Resistant Bacteria ◽

Ag Nps ◽

Antibacterial Efficiency ◽

E Coli

Silver nanoparticles (Ag-NPs) possess excellent antibacterial properties and are considered to be an alternative material for treating antibiotic-resistant bacteria. The present study was aimed at enhancing the antibacterial efficiency of Ag-NPs using visible laser light against Escherichia coli and Staphylococcus aureus in vitro. Four concentrations of Ag-NPs (12.5, 25, 50, and 100 μg/ml), synthesized by the chemical reduction method, were utilized to conduct the antibacterial activity of prepared Ag-NPs. The antibacterial efficiencies of photoactivated Ag-NPs against both bacteria were determined by survival assay after exposure to laser irradiation. The mechanism of interactions between Ag-NPs and the bacterial cell membranes was then evaluated via scanning electron microscopy (SEM) and reactive oxygen species analysis to study the cytotoxic action of photoactivated Ag-NPs against both bacterial species. Results showed that the laser-activated Ag-NP treatment reduced the surviving population to 14% of the control in the E. coli population, while the survival in the S. aureus population was reduced to 28% of the control upon 10 min exposure time at the concentration of 50 μg/ml. However, S. aureus showed lower sensitivity after photoactivation compared to E. coli. Moreover, the effects depended on the concentration of Ag-NPs and exposure time to laser light. SEM images of treated bacterial cells indicated that substantial morphological changes occurred in cell membranes after treatment. The results suggested that Ag-NPs in the presence of visible light exhibit strong antibacterial activity which could be used to inactivate harmful and pathogenic microorganisms.

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Photo-induced chemical reduction of silver bromide to silver nanoparticles

Open Chemistry ◽

10.2478/s11532-011-0085-8 ◽

2011 ◽

Vol 9 (6) ◽

pp. 982-989 ◽

Cited By ~ 5

Author(s):

Agnieszka Król-Gracz ◽

Ewa Michalak ◽

Piotr Nowak ◽

Agnieszka Dyonizy

Keyword(s):

Silver Nanoparticles ◽

Chemical Reduction ◽

Silver Bromide ◽

Molar Ratio ◽

Efficient Production ◽

Ag Nps ◽

Transmission Electron ◽

Vis Spectroscopy ◽

Nanoparticle Suspensions ◽

Actinic Radiation

AbstractThis paper discusses the experimental results of the production of nanocolloidal silver using photoreduction method. Ultrafine crystalline gelatine-stabilised aqueous suspensions of silver bromide were used as a substrate for the synthesis of silver nanoparticles (Ag NPs). The influences of the reductant to substrate molar ratio, the medium’s pH, the type of the source of actinic radiation and the time of exposure to the efficient production of the Ag NPs were studied. A typical reaction was suggested, which involves the photo-induced reduction of silver bromide nanocrystals in the presence of ascorbic acid under specified physicochemical conditions. The properties of resultant silver particles were examined using UV-Vis spectroscopy and Dynamic Light Scattering (DLS). In addition, Transmission Electron Microscopy (TEM) was used for imaging the silver nanoparticle suspensions.

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Investigation of Nanoparticle Metallic Core Antibacterial Activity: Gold and Silver Nanoparticles against Escherichia coli and Staphylococcus aureus

International Journal of Molecular Sciences ◽

10.3390/ijms22041905 ◽

2021 ◽

Vol 22 (4) ◽

pp. 1905

Author(s):

Jimmy Gouyau ◽

Raphaël E. Duval ◽

Ariane Boudier ◽

Emmanuel Lamouroux

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Silver Nanoparticles ◽

Antibacterial Activity ◽

Metallic Nanoparticles ◽

Catalytic Reduction ◽

Gold And Silver Nanoparticles ◽

High Antibacterial Activity ◽

Vis Spectroscopy ◽

Broth Microdilution Method

Multidrug-resistant (MDR) bacteria constitute a global health issue. Over the past ten years, interest in nanoparticles, particularly metallic ones, has grown as potential antibacterial candidates. However, as there is no consensus about the procedure to characterize the metallic nanoparticles (MNPs; i.e., metallic aggregates) and evaluate their antibacterial activity, it is impossible to conclude about their real effectiveness as a new antibacterial agent. To give part of the answer to this question, 12 nm gold and silver nanoparticles have been prepared by a chemical approach. After their characterization by transmission electronic microscopy (TEM), Dynamic Light Scattering (DLS), and UltraViolet-visible (UV-vis) spectroscopy, their surface accessibility was tested through the catalytic reduction of the 4-nitrophenol, and their stability in bacterial culture medium was studied. Finally, the antibacterial activities of 12 nm gold and silver nanoparticles facing Staphylococcus aureus and Escherichia coli have been evaluated using the broth microdilution method. The results show that gold nanoparticles have a weak antibacterial activity (i.e., slight inhibition of bacterial growth) against the two bacteria tested. In contrast, silver nanoparticles have no activity on S. aureus but demonstrate a high antibacterial activity against Escherichia coli, with a minimum inhibitory concentration of 128 µmol/L. This high antibacterial activity is also maintained against two MDR-E. coli strains.

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BACTERIAL CELLULOSE FROM RICE WASTE WATER AND ITS COMPOSITE WHICH ARE DEPOSITED NANOPARTICLE AS AN ANTIMICROBIAL MATERIAL

ALCHEMY Jurnal Penelitian Kimia ◽

10.20961/alchemy.v12i1.946 ◽

2016 ◽

Vol 12 (1) ◽

pp. 70 ◽

Cited By ~ 1

Author(s):

Eli Rohaeti ◽

Endang W Laksono ◽

Anna Rakhmawati

Keyword(s):

Waste Water ◽

Antimicrobial Activity ◽

Silver Nanoparticles ◽

Antibacterial Activity ◽

Bacterial Cellulose ◽

Nitrate Solution ◽

Diffusion Method ◽

E Coli ◽

Vis Spectroscopy ◽

Chitosan Composite

<pre>Bacterial cellulose (C) and its composites were synthesized from rice waste water with addition of glycerol (G) and chitosan (Ch).Antibacterial activity of the C, the bacterial cellulose-chitosan composite (CCh), and the bacterial cellulose – glycerol - chitosan composite (CGCh) which were deposited silver nanoparticles against S. aureus, E. coli, and yeast C. albicans has been conducted. Silver nanoparticles was prepared by chemical reduction of a silver nitrate solution, a trisodium citrate as a reductor, and a PVA as a stabilizer. The UV-Vis spectroscopy is used to determine the formation of silver nanoparticles. The characterization was conducted on the bacterial celluloses and those composites including the functional groups by the FTIR, the mechanical properties by Tensile Tester, photos surfaces by SEM, and the test of the antibacterial activity against S. aureus, E. coli, and C. albicans by diffusion method. The silver nanoparticle characterization indicates that the silver nanoparticles are formed at a wavelength of 418.80 nm. The antibacterial test showed an inhibitory effect of the C, the CCh, and the CGCh which are deposited the silver nanoparticles against of S. aureus, E. coli, and C.albicans. The CGChs which are deposited silver nanoparticles has the highest antimicrobial activity against the Staphylococcus aureus ATCC 25923. The CGs which are deposited silver nanoparticles provide the highest antimicrobial activity against the E. coli ATCC 25922 and the yeast Candida albicans ATCC 10231.</pre>

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Evaluation of anti bacteria effects of citrate-reduced silver nanoparticles in Staphylococcus aureus and Escherichia coli

International Journal of Research in Pharmaceutical Sciences ◽

10.26452/ijrps.v10i4.1745 ◽

2019 ◽

Vol 10 (4) ◽

pp. 3636-3643

Author(s):

Hor Jia Wei ◽

Mohd. Syafiq Awang ◽

Nor Dyana ◽

Daruliza Kernain ◽

Yazmin Bustami

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Silver Nanoparticles ◽

Antibacterial Activity ◽

Antibacterial Agent ◽

Reduction Method ◽

Surface Plasmon Resonance Peak ◽

Plasmon Resonance Peak ◽

Vis Spectroscopy ◽

Mic Value

Silver nanoparticles (AgNPs) has long known for its inhibitory and bactericidal effects. However, due to its’ attractive antibacterial property, on-going research with various synthesis strategies actively been conducted. In this study, the synthesis of AgNPs was reported, using a simple chemical reduction method with citrate as the reducing agent TEM was used to characterize the obtained AgNPs. Then, Staphylococcus aureus and Escherichia coli were used to identify the antibacterial activity of AgNPs. The inhibition effects of AgNPs against these two bacteria were observed via disc diffusion, and MIC assays and the effects of AgNPs mode of action on both bacteria were further observed under TEM. The formation of AgNPs at ̴ 400 nm, which is the surface plasmon resonance peak was observed using Uv-Vis spectroscopy. The size of AgNPs mostly in the range of 1-10 nm and their morphology appeared to be spherical. Based on the MIC assay, Escherichia coli exhibit low MIC value with 0.049 mg/ml as compared to Staphylococcus aureus with0.391 mg/ml MIC value; correspond to the effective antibacterial activity by the citrate-reduced AgNPs. Further observation on the bacterial surface structure can be seen with cross-sectional TEM image, and it provides an insight into the AgNPs mechanistic aspects of AgNPs against Staphylococcus aureus and Escherichia coli. Silver nanoparticles have been successfully synthesised using the citrate reduction method. Results obtained in this study thus elucidating promising findings to employed AgNPs as an antibacterial agent, and this composition needs to be further study and develop into an antibacterial agent.

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Photochemical synthesis of silver nanoparticles in H2O/Triton X-100/[Bmim]PF6 ionic liquid microemulsions and their antimicrobial activity

Materials Express ◽

10.1166/mex.2020.1638 ◽

2020 ◽

Vol 10 (2) ◽

pp. 267-271

Author(s):

Ranfeng Ye ◽

Min Ni ◽

Hao Chen ◽

Shengqing Li

Keyword(s):

Escherichia Coli ◽

Antimicrobial Activity ◽

Silver Nanoparticles ◽

Ionic Liquid ◽

Photochemical Synthesis ◽

Ag Nps ◽

Environment Friendly ◽

E Coli ◽

Triton X ◽

Controllable Preparation

This study presented a photoreduction route for synthesis of silver nanoparticles (Ag NPs) in ionic liquid microemulsions (ILMs). The 10 nm Ag NPs were photochemically synthesized in the H2O/Triton X-100/[Bmim]PF6 (HTB) ILMs. Above 99% Escherichia coli (E. coli) BL21 was killed in the presence of 1.56 μg/mL Ag NPs solution after incubation for 20 min, indicating that the Ag NPs prepared in HTB ILMs was applicable for the antimicrobial agent. This proposed approach for controllable preparation of Ag NPs is facile and environment-friendly, which provides great possibilities for the further functional modification of Ag NPs.

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Synergistic Antibacterial Activity of Silver-Loaded Graphene Oxide towards Staphylococcus Aureus and Escherichia Coli

Nanomaterials ◽

10.3390/nano10020366 ◽

2020 ◽

Vol 10 (2) ◽

pp. 366 ◽

Cited By ~ 8

Author(s):

Truong Vi ◽

Selvaraj Kumar ◽

Jong-Hwei Pang ◽

Yu-Kuo Liu ◽

Dave Chen ◽

...

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Bactericidal Effect ◽

Catalytic Performance ◽

Redox Activity ◽

Ag Nps ◽

E Coli ◽

Transmission Electron ◽

Physicochemical Effect

In this study, the physicochemical and surface properties of the GO–Ag composite promote a synergistic antibacterial effect towards both Gram-negative Escherichia coli (E. coli) and Gram-positive Staphylococcus aureus (S. Aureus) bacteria. GO–Ag NPs have a better bactericidal effect on E. coli (73%) and S. Aureus (98.5%) than pristine samples (pure Ag or GO). Transmission electron microscopy (TEM) confirms that the GO layers folded entire bacteria by attaching to the membrane through functional groups, while the Ag NPs penetrated the inner cell, thus damaging the cell membrane and leading to cell death. Cyclic voltammetry (CV) tests showed significant redox activity in GO–Ag NPs, enabling good catalytic performance towards H2O2 reduction. Strong reactive oxygen species (ROS) in GO–Ag NPs suggests that ROS might be associated with bactericidal activity. Therefore, the synergy between the physicochemical effect and ROS production of this material is proposed as the mechanism of its antibacterial activity.

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Synthesis and Comparative Antibacterial Activity of Fatty Acid Capped Silver Nanoparticles

Journal of Pure and Applied Microbiology ◽

10.22207/jpam.14.3.33 ◽

2020 ◽

Vol 14 (3) ◽

pp. 1941-1947

Author(s):

Rama Sharma

Keyword(s):

Silver Nanoparticles ◽

Antibacterial Activity ◽

Fatty Acid ◽

Room Temperature ◽

Reduction Method ◽

Chemical Reduction ◽

Inert Atmosphere ◽

Chemical Reduction Method ◽

Visible Spectroscopy ◽

E Coli

In the present work, the chemical reduction method has been used to synthesize silver nanoparticles using stearic acid capping agents in different concentrations. These nanoparticles are characterized by ultraviolet-visible spectroscopy and dynamic light scattering (DLS). The size of the synthesized silver nanoparticles found between 80-100 nm and stable up to 5 months. These nanoparticles show a very good bactericidal influence on E. coli and S. aureus. In this method silver nanoparticles have synthesized at room temperature without using any inert atmosphere, this is the advantage of this method over others.

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Successful in-vivo treatment of mice infected with Candida glabrata using silver nanoparticles

Bionatura ◽

10.21931/rb/2020.05.04.10 ◽

2020 ◽

Vol 5 (4) ◽

pp. 1340-1345

Author(s):

Teeba H. Mohammad ◽

Mohsen H. Risan ◽

Gamal A. El-Hiti ◽

Dina S. Ahmed ◽

Emad Yousif

Keyword(s):

Silver Nanoparticles ◽

Candida Glabrata ◽

Color Change ◽

Average Diameter ◽

Average Concentration ◽

Control Group ◽

Force Microscopy ◽

Vis Spectroscopy ◽

Ifn Γ

The current study describes the production of silver nanoparticles (AgNPs) to treat Candida glabrata infections. The method involved incubation of silver nitrate (AgNO3) with Aspergillus terreus using a green and straightforward route. The production of AgNPs was confirmed through a color change from transparent yellow to brown as well as by ultraviolet-visible (UV-VIS) spectroscopy. The surface morphology of AgNPs was assessed using a scanning electron microscope. The antifungal activity of AgNPs against C. glabrata was investigated in the serum of 20 infected mice. The mice were divided into four groups, and the level of cytokines: IL-4 and IFN-γ were examined after 21 days. The atomic force microscopy confirmed that the average diameter of AgNPs was 25.1 nm, which is appropriate for delivering silver nanoparticles to treat animals' infection. The concentration of cytokines IL-4 and IFN-γ were significantly (P < 0.05) higher in the C. glabrata-infected group than in the control group. While the cytokines level remained close to average concentration in mice administrated with AgNPs, such a result was comparable with the fourth group of mice (Candida-treated Aspergillus) after treatment with AgNPs.

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