A study was planned to ascertain effect of 50 (T1), 60 (T2) and 70 (T3) % lucerne straw in total
mixed ration (TMR) on rumen fermentation and blood metabolites in Kankrej crossbred bullocks.
Two bullocks were assigned in each treatment using 3 x 3 Latin Square design. The rumen and
blood metabolites were evaluated on last day of each period. The pH of strained rumen liquor (SRL)
was slightly alkaline (7.09-7.16) on account of green forage feeding in morning before TMR. The
pH was lowest at 4 hr post-feeding. The total volatile fatty acids (TVFAs) were 75.79-77.67 mEq/
litre and a reverse trend was observed for TVFAs as compared to pH. The values for ammonia,
total, soluble, non-protein and protein nitrogen in SRL were 15.07-17.52, 86.92-90.07, 39.90-46.55,
35.88-40.69 and 46.23-53.50 mg/100 ml, respectively. All nitrogen fractions peaked at 2 hr postfeeding,
except protein nitrogen which peaked at 6 hr, showing maximum activity up to 6 hr postfeeding.
The values for serum protein, phosphorus and calcium as well as blood haemoglobin were
within physiological range. The differences amongst treatments for rumen and blood metabolites
were non-significant. The results suggest that lucerne straw can be incorporated up to 70% in TMR
of bovines for maintenance without adverse effect on rumen fermentation and blood constituents