scholarly journals The Effectiveness of Herbal Antibacterial Formulations Against Mastitis-Causing Bacteria in Dairy Cattle

2021 ◽  
pp. 151-159
Author(s):  
Sari Suryanah ◽  
Syifa Nurjannah
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Dairy Cattle ◽  
Streptococcus Agalactiae ◽  
Animal Husbandry ◽  
Flower Extract ◽  
Turmeric Extract ◽  
Randomized Design ◽  
Inhibitory Power ◽  
Escherichia Coli Bacteria

This study aimed to determine the effectiveness of antibacterial formulations derived from herbal plants against mastitis-causing bacteria in dairy cattle. Herbal extracts composed of betel leaves extract, kecombrang flower extract, and turmeric extract were tested against Staphylococcus aureus, Streptococcus agalactiae, and Escherichia coli. The research was carried out in a Completely Randomized Design (CRD) using ten treatments and four replications at the Laboratory of Research and Testing, Faculty of Animal Husbandry, Padjadjaran University. The results showed that herbal antibacterial formulations could inhibit the growth of Staphylococcus aureus, Streptococcus agalactiae, and Escherichia coli bacteria. The most inhibitory power was obtained from P8 treatment (50% betel leaves + 50% kecombrang flower + 50% turmeric) categorized in "strong inhibition." The percentage of antibacterial inhibition of P8 formulation compared to chloramphenicol was 32% against Staphylococcus aureus, 33% against Streptococcus agalactiae, and 31% against Escherichia coli. Conclusively, the best herbal antibacterial formulation was a combination of 50% betel leaves extract, 50% kecombrang flower extract, and 50% turmeric extract.

scholarly journals Antimicrobial Susceptibility of Staphylococcus aureus, Streptococcus agalactiae, and Escherichia coli Isolated from Mastitic Dairy Cattle in Ukraine

Antibiotics ◽  
2020 ◽  
Vol 9 (8) ◽  
pp. 469
Author(s):  
Leta Elias ◽  
Ajay S. Balasubramanyam ◽  
Olena Y. Ayshpur ◽  
Iryna U. Mushtuk ◽  
Nataliya O. Sheremet ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Resistance ◽  
Dairy Cattle ◽  
Antimicrobial Susceptibility ◽  
Streptococcus Agalactiae ◽  
Bovine Mastitis ◽  
Animal Husbandry ◽  
Economic Losses ◽  
Susceptibility Profiles

Bovine mastitis is the predominant cause for antimicrobial use on dairy farms and is a major source of economic losses in the dairy industry. In this study, the antimicrobial susceptibility profiles of common mastitis-causing pathogens, Staphylococcus aureus (n = 62), Streptococcus agalactiae (n = 46), and Escherichia coli (n = 129), were determined for dairy cattle with mastitis across 142 Ukrainian farms. The results showed that there were more gentamicin resistant S. aureus isolates (16.95%) identified in this study than previously reported for Ukrainian dairy cattle. Moreover, low levels of amoxicillin susceptibly (13.51%) were observed for St. agalactiae, which contrasted a previous study showing susceptibility levels of >50%. St. agalactiae resistance to tetracycline was observed in 80% of the isolates. Cephalosporin use was most ineffective against E. coli, with 43.27–56% of the isolates exhibiting this resistant trait. Overall, this study performed a preliminary analysis of antimicrobial resistance on mastitis isolates from Ukrainian farms. However, given the limited numbers of the isolates tested in this study and that the publications on antimicrobial resistance in animal husbandry of Ukraine are very few, more extensive investigations are needed to comprehensively examine susceptibility patterns of mastitis-causing pathogens in dairy cattle in Ukraine.

scholarly journals Daya Antibakteri Ekstrak Etanol Ketepeng Cina (Senna alata) terhadap Staphylococcus aureus dan Escherichia coli secara In Vitro

2012 ◽  
Vol 13 (1) ◽  
pp. 63
Author(s):  
Taswin Yacob ◽  
Rita Endriani
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Ethanol Extract ◽  
Positive Control ◽  
Diffusion Method ◽  
Randomized Design ◽  
Senna Alata ◽  
Treatment Of Infection ◽  
Range Test

The benefit and efficacy of ketepeng cina (Senna alata) in the treatment of infection has shown that have antibacterialactivity, inhibiting and killing bacteria that cause infection. The objective of this study was evaluate the antibacterialactivity of ketepeng cina against Staphylococcus aureus dan Escherichia coli in vitro. This study was a laboratoryexperimental research which use completely randomized design with diffusion method. Ethanol extract of Sennaalata leaves devided into 4 doses, i.c. 100, 50, 25 and 12.5. Amoxiclave were used as positive control and aquadestnegative control. The data were analyzed by Analysis of Varian continued with Duncan’s Multiple Range Test. Theresult of this study showed that antimicrobial activity of ethanol extract Senna alata leaves inhibited the growth ofStaphylococcus aureus, but not Escherichia coli. The optimum effect was showed given by the concentration 100at 17.7 mm.

scholarly journals Uji Bioaktivitas Antibakteri Senyawa murni dari Jamur Endofit Sporothrix sp Terhadap Bakteri Escherichia coli dan Staphylococcus aureus

2019 ◽  
Vol 6 (1) ◽  
pp. 37
Author(s):  
Dewi Yudiana Shinta ◽  
Yusmarini Yusmarini ◽  
Herix Sonata MS ◽  
Hilwan Yuda Teruna ◽  
Saryono Saryono
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Endophytic Fungi ◽  
Positive Control ◽  
Significant Inhibition ◽  
New Drugs ◽  
Diffusion Method ◽  
Significant Difference ◽  
Pure Compounds ◽  
Escherichia Coli Bacteria

Modern medicines that are developing now come from active ingredients isolated from plants that require large amounts of plants. The development of new drugs from endophytic fungi found obstacles in the amount of pure compounds produced. Therefore further research is needed by using endophytic fungi as a new antimicrobial producer. This study aims to see the ability or activity of pure compounds produced by Sporothrix sp endophytic fungi from Dahlia tuber (Dahlia variabilis). Test the activity of pure compounds produced by Sporothrix sp. Endophytic fungi on E. coli and Staphylococcus aureus determined by disc diffusion method. With doses of 10, 30 and 50μg/disk. In Escherichia coli bacteria doses 10 and 50μg/disk gave significant inhibition of pure compounds from isolation compared to the positive control of ciprofloxacin, which was marked by a statistically significant test result (p <0.05). In contrast to Staphylococcus aureus there was no significant difference in doses of both doses of 10.30 and 50μg/disk. Determination of pure compounds was carried out by HPLC and Infra Red Spectrophotometry.

scholarly journals Possibility of application of dodicin hydrochloride in prevention of mastitis in cows

2006 ◽  
Vol 60 (3-4) ◽  
pp. 207-221
Author(s):  
Svetlana Joksovic ◽  
Vitomir Cupic ◽  
Vera Katic
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Streptococcus Agalactiae ◽  
Control Group ◽  
Main Approach ◽  
Laboratory Conditions ◽  
Streptococcus Uberis ◽  
Holstein Friesian ◽  
Experimental Group ◽  
Outer Environment

The main approach to curbing mastitis is to prevent the entry of microorganisms from the outer environment into the mammary gland, which is achieved by the use of papilla disinfection following every time of milking. The objective of this work was to examine the antimicrobial activity of the disinfectant dodicin hydrochloride, in fact the preparation that contains this disinfectant (DESU? M), against bacteria, the most frequent causes of mastitis in cows. The efficacy of modified DESU? Mwas examined under laboratory conditions using the quantitative test against the following microorganisms: Escherichia coli, Staphylococcus aureus, Streptococcus agalactiae and Streptococcus uberis, under the conditions in the field. The udders of an experimental group of 20 cows of the Holstein-Friesian breed were immersed in a solution of the modified preparation DESU ? M following every time of milking over a period of three months. In the second group of 10 cows of the Holstein-Friesian breed, marked as the control group, no disinfection was applied following the milkings. The preparation DESU? Mexhibited satisfactory antimicrobial efficacy against the most frequent causes (Escherichia coli, Staphylococcus aureus, Streptococcus agalactiae and Streptococcus uberis) of mastitis in cows under laboratory conditions. With the application of the preparation DESU? M, following every time of milking over a period of three months, the number of somatic cells was reduced by almost one half in comparison with their number at the start of the experiment. No residue of the modified preparation DESU ? M were found in any sample of milk from the experimental group of cows.

scholarly journals SINTESIS NANOKOMPOSIT Nata de coco/TiO2/Ag DAN EFEKTIVITASNYA SEBAGAI ANTIBAKTERI TERHADAP BAKTERI Escherichia coli dan Staphylococcus aureus

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Heriyanto Tinentang ◽  
Henry F Aritonang ◽  
Harry S. J. Koleangan
Keyword(s):  
Electron Microscopy ◽  
Escherichia Coli ◽  
Scanning Electron Microscopy ◽  
Staphylococcus Aureus ◽  
Chemical Reduction ◽  
Bacterial Activity ◽  
Energy Dispersive ◽  
X Ray ◽  
Escherichia Coli Bacteria ◽  
Scanning Electron

Telah dilakukan penelitian tentang kemampuan aktivitas anti bakteri untuk bakteri Staphylococcus aureus (gram positif) dan Escherichia coli (gram negatif) dengan menggunakan nanokomposit nata de coco/TiO2, nata de coco/Ag, dan nata de coco/TiO2/Ag dengan variasi konsentrasi Ag 0,5 M; 0,6 M; 0,7 M; 0,8 M dan 0,9 M  menggunakan metode reduksi kimia. Nanopartikel tersebut dikarakterisasi menggunakan X-Ray Diffractometry (XRD), scanning electron microscopy-energy dispersive X-ray spectroscopy  (SEM-EDS) dan anti bakteri. Untuk uji aktivitas antibakteri menggunakan metode kertas cakram dan dilakukan sebanyak dua kali ulangan untuk tiap-tiap sampel dan bakteri yang diuji. Hasil penelitian menunjukan, aktivitas anti bakteri nanokomposit yang paling baik dalam menghambat pertumbuhan bakteri adalah nanokomposit Nata de coco/TiO2/Ag mampu menghambat pertumbuhan bakteri Escherichiacoli dan Staphylococcusaureus, namun nanokomposit tersebut lebih efektif menghambat pertumbuhan bakteri Escherichiacoli.ABSRACT Research on the ability of anti-bacterial activity for Staphylococcus aureus (gram positive) and Escherichia coli (gram negative) bacteria using nata de coco / nanocomposites TiO2, nata de coco / Ag, and nata de coco / TiO2 / Ag with variations of Ag 0,5 M; 0.6 M; 0.7 M; 0.8 M and 0.9 M using the chemical reduction method. Nanoparticles were characterized using X-Ray Diffractometry (XRD), scanning electron microscopy-energy dispersive X-ray spctroscopy  (SEM-EDS) and anti-bacterial actvity. Test the antibacterial activity using the paper disc method and repeated two times for each sample and bacteria tested. The results showed that the good anti-bacterial activity of nanocomposites in inhibiting bacterial growth was nanocomposite nata de coco /TiO2/Ag  able to inhibit the growth of Escherichia coli and S. aureus, but the nanocomposite is more effective in inhibiting the growth of Escherichia  coli bacteria.

scholarly journals ANTIBAKTERI SENYAWA FENOLIK DARI ALANG -ALANG (IMPERATA CYLINDRICA)

2021 ◽  
Vol 3 (2) ◽  
pp. 124-126
Author(s):  
Jhon Patar Sinurat ◽  
Suci Wulandari ◽  
Rinaldo Berutu
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Phenolic Compounds ◽  
Screening Test ◽  
Inhibition Zone ◽  
Imperata Cylindrica ◽  
Diffusion Method ◽  
Phytochemical Screening ◽  
Agar Diffusion Method ◽  
Escherichia Coli Bacteria

Phytochemical screening test proved that the extract of Reeds (Imperata cylindrica) contained phenolic compounds tested using 5% FeCl3 reagent. Antibacterial test using agar diffusion method against Reeds extract in DMSO solvent. The phenolic compounds obtained from the saputangan leaves Reeds extract were 36.96 grams after undergoing maceration. Reeds extract of phenolic compounds with concentrations (200; 100; 50 and 25 ppm) had strength antibacterial where the average inhibition zone of Staphylococcus aureus bacteria was 10.0 mm and the average inhibition zone was at Escherichia coli bacteria measuring 10.3 mm.

Absence of Bacterial Adherence in the Establishment of Experimental Mastitis in Mice

1978 ◽  
Vol 15 (6) ◽  
pp. 770-775 ◽  
Author(s):  
J. C. Anderson
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Mammary Gland ◽  
Significant Role ◽  
Streptococcus Agalactiae ◽  
Staphylococcus Epidermidis ◽  
Alveolar Epithelium ◽  
Bacterial Adherence ◽  
E Coli ◽  
Suckling Mice

The possibility of adherence of Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli or Streptococcus agalactiae to the epithelium of the mammary gland was investigated by inoculating them into this gland of mice. S. aureus, S. epidermidis and E. coli did not adhere to alveolar epithelium in suckling or non-suckling mice. S. agalactiae adhered to alveolar epithelium in non-suckling mice but adhesion was not sufficiently strong to withstand suckling. Bacterial adherence probably does not play a significant role in the establishment of mastitis by these organisms.

scholarly journals Izolacija bakterijskih patogena kod klinički manifestnih mastitisa mliječnih goveda i njihova antimikrobna osjetljivost u zeničkoj regiji u 2017. godini

2020 ◽  
Vol 51 (1) ◽  
pp. 47-52
Author(s):  
Jasmin Burović
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Klebsiella Pneumoniae ◽  
Yersinia Enterocolitica ◽  
Streptococcus Agalactiae ◽  
Enterococcus Spp

Ova studija predstavlja podatke prikupljene iz Veterinarske stanice “Zevet” d.o.o. Zenica, Bosna i Hercegovina o zastupljenosti bakterijskih patogena kod klinički prisutnih mastitisa mliječnih goveda i profilima njihove antimikrobne osjetljivosti. Za laboratorijsku izolaciju obrađena su 52 uzorka od kojih su 23 bila bakteriološki pozitivna. Najčešći izolati bili su Staphylococcus aureus u 21,74 % uzoraka i Streptococcus agalactiae u 17,39 % izolira- nih uzoraka, koagulaza-negativni stafilokoki (13,04 %), Klebsiella pneumoniae (13,04 %), Ente- rococcus spp. (8,70 %), Escherichia coli (8,70 %), Streptococcus spp., Enterobacter, Serratia spp. i Yersinia enterocolitica (4,35 %). Najveća antimikrobna otpornost među izolatima primjećena je na benzilpenicilin (56,25 %) i oksitetraciklin (46,15 %) s ukupnom otpornosti na uporabljena antimikrobna sredstava od 25,99 %. Na tri testirana antimikrobna sredstva (neomicin, klindamicin i cefotaksim) izolati nisu pokazali otpornost. Ostali antibiotici na koje je primijećena niska ukupna otpornost su ciprofloksacin (5,56 %) i ofloksacin (6,67 %). Samo jedan izolat nije pokazao nikakvu otpornost na testirane antimikrobike (S. aureus). Najveću otpornost među izolatima pokazao je Enterococcus spp., pokazujući senzibilitet samo za tetracikline. S. aureus je pokazao otpornost na benzilpenicilin i cefaleksin u 60 % slučajeva. Svi izolati S. agalactiae su bili otporni na tetracikline, oksitetracikline i gentamicin. Koagulaza negativni stafilokoki pokazali su otpornost na cefaleksin u dva slučaja i otpornost na benzilpenicilin, eri- tromicin i oksacilin u jednom izolatu. Klebsiella pneumoniae je pokazala otpornost na cefaleksin, amoksicilin i benzilpenicilin u svim ispitivanim izolatima, a pokazala je i otpornost na tetracikline u dva izolata. Svi ostali izolati pokazali su otpornost prema najmanje jednom testiranom antimikrobiku. U 46 slučajeva se odmah pristu- palo liječenju s 86,96 % uspjehom koji je procijenjen na osnovu izostanka kliničkog relapsa i smanjenja broja somatskih stanica u dopuštene granice za tri tjedna nakon provedene terapije.

scholarly journals LambdaSa1 and LambdaSa2 Prophage Lysins of Streptococcus agalactiae

2007 ◽  
Vol 73 (22) ◽  
pp. 7150-7154 ◽  
Author(s):  
David G. Pritchard ◽  
Shengli Dong ◽  
Marion C. Kirk ◽  
Robert T. Cartee ◽  
John R. Baker
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Cell Wall ◽  
Streptococcus Agalactiae ◽  
Synthetic Peptide ◽  
Cell Walls ◽  
Peptide Substrate ◽  
Amidase Activity ◽  
Cleavage Specificity ◽  
Endopeptidase Activity

ABSTRACT Putative N-acetylmuramyl-l-alanine amidase genes from LambdaSa1 and LambdaSa2 prophages of Streptococcus agalactiae were cloned and expressed in Escherichia coli. The purified enzymes lysed the cell walls of Streptococcus agalactiae, Streptococcus pneumoniae, and Staphylococcus aureus. The peptidoglycan digestion products in the cell wall lysates were not consistent with amidase activity. Instead, the structure of the muropeptide digestion fragments indicated that both the LambdaSa1 and LambdaSa2 lysins exhibited γ-d-glutaminyl-l-lysine endopeptidase activity. The endopeptidase cleavage specificity of the lysins was confirmed using a synthetic peptide substrate corresponding to a portion of the stem peptide and cross bridge of Streptococcus agalactiae peptidoglycan. The LambdaSa2 lysin also displayed β-d-N-acetylglucosaminidase activity.

scholarly journals UJI FITOKIMIA DAN DAYA HAMBAT EKSTRAK DAUN JUWET (Syzygium cumini) TERHADAP PERTUMBUHAN Escherichia coli DAN Staphylococcus aureus ATCC

2017 ◽  
Vol 5 (2) ◽  
pp. 47 ◽  
Author(s):  
Kadek Sudarmi ◽  
Ida Bagus Gede Darmayasa ◽  
I Ketut Muksin
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Leaf Extract ◽  
Syzygium Cumini ◽  
E Coli ◽  
Randomized Design ◽  
Test Concentration ◽  
Completely Randomized Design ◽  
Difference Test ◽  
Test Level

Bacterial Escherichia coli dan Staphylococcus aureus ATCCis a normal flora bacteria that naturally exist human body. This bacteria may by pathogenic if it exceeds acertain limits. To prevent the disease caused, need to be overcome by using herbal ingredients one of them is juwet leaves (Syzygiumcumini).The purpose of the study was to determine wheter the extract of juwet leaf (S. cumini) can inhibited the growth of E. coli and S. aureus ATCC and to know compounds contained in juwet leaf extract (S. cumini). This research used diffusion wells methodwith 6 treatments and 4 replications. Date analysis using completely randomized design (CRD) with using ANOVA variance analysis. If the date obtained has a real difference test level at the 5% (P? 0,5) that continue with Duncan test. Concentration of the extract tested was 0%, 5%, 10%, 15%, 25% and 50%. Juwet leaf extract is able to inhibited the growth of E. coli and S. aureus ATCC which is shown by the formation of clear zone. Concentration extract leaf which is effective to inhibit E. coli and S. aureus ATCC is the concentration 50% (18,9 mm) for E. coli and (16,5 mm) for S. aureus ATCC. Screening of phytochemical test of juwet leaf extract positive containing alkaloids, phenolic, steroids and saponins.   Keywords : juwet leaf extract (Syzygium cumini), Escherichia coli, Staphylococcus aureus ATCC  

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