scholarly journals GC-MS analysis of bioactive compounds in ethanolic leaf extract of Hellenia speciosa (J.Koenig) S.R.Dutta

2021 ◽  
Author(s):  
Ramya Raveendran
Keyword(s):  
Methyl Ester ◽  
Bioactive Compounds ◽  
Leaf Extract ◽  
Acid Methyl Ester ◽  
Acid Methyl ◽  
Ms Analysis ◽  
The Family ◽  
Intestinal Worms ◽  
Phytochemical Components ◽  
Ethanolic Leaf Extract

Abstract Hellenia speciosa (J.Koenig) S.R.Dutta, is a plant species belonging to the family Costaceae. It is widely distributed in China, India, Malaysia, Indonesia, tropical and subtropical Asia. In Ayurveda, the rhizome of the plant has been extensively used to treat fever, rash, asthma, bronchitis and, intestinal worms. The objective of the present study was to investigate the phytochemical constituents of the leaf of Hellenia speciosa using Gas Chromatography and Mass Spectroscopy analysis (GC-MS). The GC-MS analysis revealed the presence of 17 phytochemical components in the ethanolic leaf extract of Hellenia speciosa. The prevailing bioactive compounds present in Hellenia speciosa were Thymol (RT-10.019;3.59%), Caryophyllene (RT-11.854:0.62%), Caryophyllene oxide (RT-13.919;1.34%), Artumerone (RT-14.795;1.35%), Hexadecanoic acid methyl ester (RT-17.536; 2.77%), 9,12-Octadecanoic acid methyl ester (RT-19.163;1.35%), Squalene (RT- 24.980;1.19%), Piperine (RT-25.745;3.11%), Beta Tocopherol (RT-26.681;2.88%) Vitamin E (RT- 27.290;2.64%), Progesterone (RT-29.608;3.18%), Caparratriene (RT-29.861;9.72%), and Testosterone (RT-30.73;5.81%). The compounds were identified by comparing their retention time and peak area with that of the literature and by interpretation of mass spectra. The results and findings of the present study suggest that the plant can be used as a valuable source in the field of herbal drug discovery. The presence of bioactive compounds justifies the use of plant leaves for treating various diseases with fewer side effects and recommended the plant of pharmaceutical importance. However, further studies are needed to undertake its bioactivity and toxicity profile.

scholarly journals Bioactive Variability and In Vitro and In Vivo Antioxidant Activity of Unprocessed and Processed Flour of Nine Cultivars of Australian lupin Species: A Comprehensive Substantiation

Antioxidants ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 282 ◽  
Author(s):  
Kishor Mazumder ◽  
Afia Nabila ◽  
Asma Aktar ◽  
Asgar Farahnaky
Keyword(s):  
Antioxidant Activity ◽  
Methyl Ester ◽  
Bioactive Compounds ◽  
Antioxidant Activities ◽  
Acid Methyl Ester ◽  
Heat Processing ◽  
Acid Methyl ◽  
Ms Analysis

The aim of this present investigation was to analyze bioactive compounds, as well as demonstrate the antioxidant activities of nine cultivars of Australian lupin species accompanied by observing the effect of domestic heat processing on their antioxidant activities adopting in vivo and in vitro approaches. Gas chromatography mass spectroscopy (GC-MS) analysis was performed for profiling bioactive compounds present in lupin cultivars. Multiple assay techniques involving quantification of polyphenolics, flavonoids and flavonol, electron transfer (ET) based assay, hydrogen atom transfer (HAT)-based assay and in vivo assays were performed. The major compounds found were hexadecanoic acid methyl ester, 9,12-octadecadienoic acid methyl ester, methyl stearate, lupanine,13-docosenamide and 11-octadecenoic acid (Z)- methyl ester. Mandelup was found to show excellent antioxidant activity. Moreover, Jurien, Gunyidi and Barlock had strong antioxidant activity. Both positive and negative impacts of heat processing were observed on antioxidant activity. Heating and usage of excess water during processing were the key determinants of loss of antioxidants. Negligible loss of antioxidant activity was observed in most of the assays whereas inhibition of both lipid peroxidation (33.53%) and hemolysis of erythrocytes (37.75%) were increased after processing. In addition, in vitro and in vivo antioxidant assays are found to show statistically significant (* p < 0.05 and ** p < 0.01) results, which are supported by the presence of a number of antioxidant compounds in GC-MS analysis.

Identification of potential targets for Thymidylate Synthase and Amp-C βlactamase from of non-alkaloidal fractions of Moringa oleifera leaves

2021 ◽  
Vol 22 ◽  
Author(s):  
Chandresh Kumari ◽  
Amanpreet Kaur Virk ◽  
Swati Kumari ◽  
Tanvi Gupta ◽  
Rajan Rolta ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Methyl Ester ◽  
Thymidylate Synthase ◽  
Leaf Extract ◽  
Moringa Oleifera ◽  
Acid Methyl Ester ◽  
Hexadecanoic Acid ◽  
Acid Methyl ◽  
Moringa Oleifera Leaves ◽  
Ethanolic Leaf Extract

Aim: The aim of present investigation is to identify the potential targets for Thymidylate Synthase and Amp-C βlactamase from of non-alkaloidal fractions of Moringa oleifera leaves. Background: Bioactive constituents from medicinal plants, either as pure compounds or as crude form, provide vast opportunities for new drug discoveries. Due to an increasing demand for chemical diversity in screening programs, seeking therapeutic drugs from natural products, mainly from edible plants, has grown throughout the world. Moringa oleifera has an impressive range of medicinal uses with high nutritional value. Therefore, this medicinal plant has been used widely in traditional Indian medicine for anti-inflammation, anticancer, and antibacterial infections. Methods: In the present investigation, ethanolic extract of Moringa leaves was prepared and then fractionated on the presence/absence of alkaloids. The antimicrobial activity of different fractions of ethanolic leaf extract was evaluated against various pathogens. After this, bioactive molecules present in the non-alkaloidal fractions of ethanolic leaf extract were accomplished through GC-MS analysis and finally the identified phytocompounds were analyzed through docking studies to evaluate their affinity for target proteins viz. Thymidylate Synthase (1HVY) and Amp-C β-lactamase (1FSY). Results: The antimicrobial activity of non-alkaloidal fractions of ethanolic leaf extract was evaluated against various pathogens which exhibited significant antimicrobial activity. Twenty phytocompounds were identified as gas chromatogram of the non-alkaloidal fractions (chloroform and ethyl acetate) of leaf extract of M. oleifera, Four most prominent compounds having highest peak area percentage were identified as Ethane, 1,1,2,2-tetrachloro, (46.45%) 2-Propanone, 1,1,3-trichloro, (13.77%) Heptasiloxane, 1,1,3,3,5,5,7,7,9,9,11,11,13,13-tetradecamethyl (17.87%) and 2,4-Dichlorodiphenylsulfone (17.64%). Other notable compounds were 9,12-Octadecadienoic acid (Z,Z) (14.06%), Oleic acid, 3-(octadecyloxy)propyl ester (12.41%), Fluoranthene (6.98%), Phenol, 2,4-bis( 1,1-dimethylethyl) (4.16%) and Phthalic acid, butyl nonyl ester (3.47%). Only, five compounds viz. 2,6-Bis(1,1-dimethylethyl)phenol(C1), Dodecamethylcyclohexasiloxane(C2), Chlorodimethylethylsilane(C3), Fluoranthene(C4) and Hexadecanoic acid, methyl ester(C5) showed the maximum interaction with 1HVY with highest docking score of -178.51Kcal/mol, - 231.65Kcal/mol, -129.18Kcal/mol, - 173.10Kcal/mol and -220.78Kcal/mol, respectively. Also, three compounds viz. Dodecamethylcyclohexasiloxane(C2), Fluoranthene(C4) and Hexadecanoic acid, methyl ester(C5) showed the maximum interaction with 1FSY with highest docking score of -137.23Kcal/mol, -54.34Kcal/mol and -153.84Kcal/mol, respectively. Conclusion: Moringa plant may provide incredible capabilities to develop pharmacological products. The present finding demonstrated that Moringa oleifera is an excellent plant candidate to be used for improving the health of communities.

Identification of Potential Antimicrobial Compounds from A Marine Streptomyces Sp. SM2.4 Strain (MH752437) Isolated from Rachgoun Island in Western Algeria

2021 ◽  
Vol 17 ◽  
Author(s):  
Nesrine Boublenza ◽  
Nadir Boudjlal Dergal ◽  
Larbi Belyagoubi ◽  
Noujoud Gabed ◽  
Djamel-Eddine Abdelouahid ◽  
...  
Keyword(s):  
Methyl Ester ◽  
Bioactive Compounds ◽  
Crude Extract ◽  
Acid Methyl Ester ◽  
Bioactive Metabolites ◽  
Streptomyces Sp ◽  
Acid Methyl ◽  
Ms Analysis ◽  
Western Algeria ◽  
Marine Streptomyces

Background: Marine actinobacteria are a potential resource for natural products; their secondary bioactive metabolites have shown several biological activities. Most of the isolated and identified actinobacteria in Algeria were usually explored from caves, Saharan soil or palm groves. The marine ecosystem is poorly explored and documented. Methods: Five Streptomyces strains producing bioactive compounds were isolated from Rachgoun Island located in Western Algeria and characterised phenotypically and genotypically using microbiological and 16S rRNA sequencing methods, respectively. The crude extract of the most representative strain “Streptomyces sp. strain SM2.4” and its seven active fractions were characterised by GC/MS analysis. Results: Streptomyces sp. strain SM2.4 revealed the strongest activity against the Gram-positive bacteria Staphylococcus aureus and Bacillus subtilis, the fungus Aspergillus niger and was inactive against Gram-negative bacteria. GC/MS analysis of the methylated crude extract of Streptomyces sp. strain SM2.4 revealed the presence of 11 major compounds including fatty acids methyl ester (12-methyltridecanoic acid methyl ester, 9-hexadecenoic acid methyl ester, hexadecanoic acid methyl ester, 14-methylhexadecanoic acid methyl ester and 16-methylheptadecanoic acid methyl ester), 2,4-di-tert-butylphenol, (4S,4aS,8aR)-4,8a-dimethyloctahydro-4a(2H)-naphthalenol (geosmin), 2,4-dimethylbenzaldehyde, 3,4-difluorobenzaldehyde, dimethylfuran-2,4-dicarboxylate and pyrrolo(1,2-a)pyrazine-1,4-dione, hexahydro-3-(2-methylpropyl)-. Partial purification of the crude extract by Thin-layer chromatography provided seven active fractions which were tested by radial diffusion assay. GC/MS analysis of the active TLC-fractions revealed the presence of a mixture of active compounds from which 2-(bromomethyl)-2-(2-methylphenyl)-1,3-dioxolane was found to be a new 1,3 dioxolane derivative. Furthermore, 3,4-dimethylbenzamide and pyrido[2,3-d] pyridazine-1,4-dione, hexahydro-3-(2-methylpropyl)-, were extracted for the first time from a natural source. Conclusion: Our study reveals that marine Streptomyces sp. strain SM2.4 has an interesting antimicrobial potential due to its panel of bioactive compounds.

scholarly journals Antioxidant Potential of Crude Methanol Leaf extract and Fraction of Mallotus oppositifolius

2021 ◽  
pp. 168-184
Author(s):  
Ifeanyi Peter Onyeka ◽  
Felix Ahamefule Onyegbule ◽  
Christopher Obodike Ezugwu ◽  
Chibueze Jeremiah Ike ◽  
Chidalu B. Ikeotuonye
Keyword(s):  
Methyl Ester ◽  
Ethyl Acetate ◽  
Crude Extract ◽  
Leaf Extract ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Acid Methyl Ester ◽  
Ethyl Acetate Fraction ◽  
Antioxidant Potential ◽  
Acid Methyl

Mallotus oppositifolius (Geiseler) Mull. Arg.  (Euphorbiaceae) is a predominant edible shrub in Nigeria. It is locally used for treatment of common infections and wounds. This study evaluated the toxicity and antioxidant potential of the crude extract and fractions of M. oppositifolius using invitro 1,1-diphenyl-2-picrylhydrazine (DPPH) radical scavenging assay. The result showed abundance of flavonoids (28.21), tannins (17.72), alkaloids (7.79) and Saponins (0.23) in the crude extract of M. oppositifolius leaf extract. Also, the result showed that the LD50 is above 5000 mg/kg, therefore the crude extract is safe for consumption. The result further showed that the ethyl-acetate and butanol had significant (p<0.05) antioxidant activity when compared with the control at 125, 62.5 and 31.125 µg/ml respectively while at 250 and 500 µg/ml there was no significant different in the level of reduction of oxidation between the butanol and ethyl acetate when compared with the control. The result further showed that the butanol and ethyl-acetate fraction of M. oppositifolius had the best antioxidant potential among the fractions studied. The result of GCMS studies of ethyl-acetate fraction showed the presence of 7,10,13-eicosatrienoic acid methyl ester, korormicin, agosterol, phyllanthin, ricinoic acid, 2,4,6-decatrienoic acid 1a,2,5,5a,6,9,10,10a-octahydro-5,5a-dihydroxy-4-(hydroxymethyl)-1,1,7,9, limonin, com-pounds that have shown to possess strong antioxidant activities. Also, the GCMS result of butanol fraction showed the presence of seven compounds which include: ferulylhistamine, hippeastrine, 10,13-eicosadienoic acid methyl ester, ethyl 5,8,11,14-eicosatetraenoate, 6-hydroxy-powelline-N-nitroso-7-demethoxy-aldehyde, 6,7-epoxypregn-4-ene-9,11,18-triol-3,20-dione 11,18-diacetate and 5Alpha-androstane-3,17-dione 17-monooxime,  compounds which may serve as a new lead for the treatment of diseases associated with the oxidative stress.

scholarly journals GC-MS ANALYSIS PROFILE AND BIOACTIVE COMPONENTS OF FLOWERS OF Bergenia ciliata, A WEED OF ROCK CREVICES

2021 ◽  
Vol 27 (4) ◽  
pp. 527-535
Author(s):  
Malik F. H. Ferdosi ◽  
Iqra Haider Khan ◽  
Arshad Javaid ◽  
Hafiz M. Saeed ◽  
Ifrayeem Butt ◽  
...  
Keyword(s):  
Methyl Ester ◽  
Octadecadienoic Acid ◽  
Acid Methyl Ester ◽  
Literature Survey ◽  
Pentadecanoic Acid ◽  
Bioactive Components ◽  
Hexadecanoic Acid ◽  
Acid Methyl ◽  
Ms Analysis ◽  
Hexanedioic Acid

Bergenia ciliata is a weed of hilly areas of Pakistan that generally grows in rock crevices. In the present study, flowers of this weeds were collected from Murree. The dried flowers were extracted in methanol and subjected to GC-MS analysis that showed 7 compounds in it. The predominant compound was hexanedioic acid, bis(2-ethylhexyl) ester (48.88%) followed by γ-sitosterol (22.56%). Moderately occurring compound was cyclohexane, 1,3,5-triphenyl- (12.87%). The remaining compounds namely n-hexadecanoic acid (4.97%), pentadecanoic acid, 14-methyl-, methyl ester (3.77%), 9,12-octadecadienoic acid, methyl ester, (Z,Z)-  (2.94%) and 9,12,15-octadecatrienoic acid, methyl ester, (Z,Z,Z)- (2.92%) were categorized as less abundant compounds. Literature survey indicated that these compounds possess antimicrobial, larvicidal, anti-inflammatory, anticancer and/or antidiabetic properties.

scholarly journals GC-MS Analysis of the Fatty Acid Methyl Ester in Japanese Quail Fat

2015 ◽  
Vol 10 (2) ◽  
pp. 54-57
Author(s):  
Ion Dragalin ◽  
Olga Morarescu ◽  
Maria Sedcenco ◽  
Radu Marin Rosca
Keyword(s):  
Fatty Acid ◽  
Methyl Ester ◽  
Fatty Acid Methyl Ester ◽  
Japanese Quail ◽  
Acid Methyl Ester ◽  
Acid Methyl ◽  
Ms Analysis

scholarly journals GC-MS ANALYSIS OF BIO-ACTIVE COMPOUNDS FROM THE ETHANOLIC EXTRACT OF BALIOSPERMUM MONTANUM (WILD.) MUELL. ARG.

2018 ◽  
Vol 5 (1) ◽  
pp. 42-45
Author(s):  
Reshma K ◽  
Baluprakash T ◽  
Abdul Kaffoor H ◽  
Venkatachalapathi A ◽  
Arumugasamy K
Keyword(s):  
Methyl Ester ◽  
Medicinal Plant ◽  
Bioactive Compounds ◽  
Mass Spectra ◽  
Octadecadienoic Acid ◽  
Acid Methyl Ester ◽  
Ethanolic Extract ◽  
Acid Methyl ◽  
Pharmacological Studies ◽  
Phytochemical Compounds

Baliospermum montanum (Wild.) Muell. Arg., (Euphorbiaceae) is a well known medicinal plant which is used in treatment of various diseases. The present study was focussed on the separation and investigation of the phytochemical compounds from ethanolic extract by GC-MS technique. The mass spectra of thecompounds found in the extract were matched with the National Institute of Standards and Technology (NIST) library. The ethanolic extract revealed the presence of 30 bioactive compounds. The major and minor phytochemical compounds are 2,15-Dithia[3](9,10)anthracenol[3](2,6)pyridinophane,1,2,3,4-tetrahydro showed the highest peak 9.32% followed by phytol-9.08%, Neophytadiene-7.38%, 8,11-Octadecadienoic acid, methyl ester (CAS)-7.06%, 4-ethyl-6-[2-(methoxycarb onyl)ethenyl]-7-[2-(methoxycarbonyl)ethyl]1,3,5,8-tetramethyl-2-vinylporphyrin-6.72%, 2-pentafluorophenylpropanal-6.43%,4,4'-Isopropylidene-bis-(2-cyclo hexyl phenol)-5.91% etc.. Further pharmacological studies are needed to find out the medicinal aspect of these compounds.

Bioactive Compounds Investigation From Methanol Bark Extract of Pterocarpus marsupium Using GC-MS Analysis

2017 ◽  
Vol 8 (03) ◽  
Author(s):  
Aruna L. Hugar ◽  
Amarvani P. Kanjikar ◽  
Ramesh L. Londonkar
Keyword(s):  
Methyl Ester ◽  
Bioactive Compounds ◽  
Acid Methyl Ester ◽  
Bark Extract ◽  
Therapeutic Effects ◽  
Octadecanoic Acid ◽  
New Novel ◽  
Ms Analysis ◽  
Novel Drug ◽  
Therapeutic Activities

Introduction: Medicinal plants still represent untapped sources of novel compounds with potential therapeutic effects for mitigating human maladies.Pterocarpus marsupium, commonly known as Bijasal, is a well-known drug in ayurvedic system of medicine. Materials and methods: The dried bark powder of P.marsupium was extracted by Soxhlet with methanol and analyzed by gas chromatography, while the mass spectra data of the compounds found in the extract was matched with the data available at National Institute of Standards and Technology (NIST) library. Results: The results of the GC-MS analysis provide different peaksdetermining the presence of 10 major phytocomponents, that are 1-Penten-3-one,1-(2,6,6-trimethyl-1-cyclohexen-1-yl)-; Geranyl isovalerate; 2-Pentadecanone,6, 10, 14-trimethyl;Cyclopropaneoctanal, 2-octyl-; Octadecanoic acid;10-Octadecanoic acid, methyl ester; Heptadecanoic acid, 16-methyl,-methyl ester; Phytol; 4,8,12,16-Tetramethyl heptadecan-4-olide; Dasycarpidan-1-methanol, acetate with different therapeutic activities. Conclusion: The presence of these bioactive compounds justifies that the Pterocarpus marsupiumcan offer a base for using this compound as herbal alternative for the synthesis and development of new drug to treat various infectious diseases. However, isolation of individual phytochemical constituents might be useful to formulate a new novel drug.

N-Acetylborrelidin B: a new bioactive metabolite from Streptomyces mutabilis sp. MII

2018 ◽  
Vol 73 (1-2) ◽  
pp. 49-57 ◽  
Author(s):  
Abdelaaty Hamed ◽  
Ahmed S. Abdel-Razek ◽  
Marcel Frese ◽  
Daniel Wibberg ◽  
Atef F. El-Haddad ◽  
...  
Keyword(s):  
Linoleic Acid ◽  
Methyl Ester ◽  
Bioactive Compounds ◽  
Screening Program ◽  
Acid Methyl Ester ◽  
In Vitro Cytotoxicity ◽  
Cervix Carcinoma ◽  
Nmr Studies ◽  
Acid Methyl

AbstractIn the course of our screening program for new bioactive compounds, a naturally new 18-membered macrolide antibiotic,N-acetylborrelidin B (1) along with borrelidin (2) were obtained from the marineStreptomyces mutabilissp. MII. The strain was isolated from a sediment sample collected in the Red Sea at the Hurghada Coast and characterized taxonomically. Additional nine diverse bioactive compounds were reported: 6-prenyl-indole-3-acetonitrile (3), sitosteryl-3β-d-glucoside, campesterol, ferulic acid, linoleic acid methyl ester, linoleic acid,N-acetylanthranilic acid, indole 3-acetic acid methyl ester, indole 3-carboxylic acid, and adenosine. Structure1was confirmed by in-depth NMR studies and by mass spectra, and comparison with related literature data. The antimicrobial activity of the strain extract and compounds1and2were studied using a panel of pathogenic microorganisms. The in vitro cytotoxicity of compounds1and2as well as the crude extract were tested against the human cervix carcinoma cell line (KB-3-1).

scholarly journals Bioassay-guided Identification of Bioactive Compounds from Senna alata L. against Methicillin-resistant Staphylococcus aureus

2021 ◽  
Author(s):  
Suwaiba Muhammad Ladan ◽  
Yusuf Wada ◽  
Mustapha Mohammed ◽  
Sani Ibrahim ◽  
Kabir Musa Yusuf ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Methyl Ester ◽  
Bioactive Compounds ◽  
Diethyl Ether ◽  
Octadecenoic Acid ◽  
Acid Methyl Ester ◽  
Public Health Problem ◽  
Methicillin Resistant ◽  
Acid Methyl ◽  
Senna Alata

Senna alata (Linn) Roxb. plant is widely used to manage various infections in folkloric medicine. Methicillin-resistant Staphylococcus aureus (MRSA) infection continues to be a major global public health problem. This study aims to investigate the bioactive components of S. alata leaves active against MRSA. The leaves of S. alata were sequentially extracted and fractionated using standard methods and screened for activities against MRSA. The diethyl ether active thin layer chromatography (TLC) spot was subjected to infrared (IR) and gas chromatography-mass spectroscopic (GC-MS) studies. The aqueous extract and diethyl ether fraction of S. alata leaves elicited the highest activity against the MRSA. The GC-MS analysis of the fraction produced 15 eluates; only the sub-fraction 13 was effective. The TLC analysis of the sub-fraction 13 revealed three spots; only the second spot produced activity. The GC-MS result of the spot showed six peaks. The spectral results for peak 3 match the data from the IR study suggestive of 9-octadecenoic acid methyl ester. Senna alata leaves possess bioactive compounds closely related to 9-octadecenoic acid methyl ester with potent antibacterial activity against MRSA.

Sign in / Sign up

Export Citation Format

Share Document