Interactions of Flavone and Steroid from A. subintegra as Potential Inhibitors for Porcine Pancreatic Lipase

Background: Obesity is one of serious health condition in the world which contributes too many of chronic diseases. The inhibition of pancreatic lipase is one of promising treatment of obesity problem. <p> Objective: The present study was designed to investigate anti-porcine pancreatic lipase effect of isolated compounds from Aquilaria subintegra and its mechanism. <p> Method: The isolation of crude extract was used serial column chromatography and identified using spectroscopic methods. Then the isolated compounds were assayed for anti-lipase properties using colorimetric assay. The prediction of energy binding between isolated compounds and enzyme was described using YASARA software. <p> Results: Four compounds were successfully isolated from bark of A. subintegra, namely, 5-hydroxy-7,4’-dimethoxyflavone, luteolin-7,3’,4’-trimethyl ether, 5,3’-dihydroxy-7,4’-dimethoxyflavone and β-sitosterol. The results indicated that all compounds were promise to inhibit pancreatic lipase activity in range between of 6% to 53%. This study showed that 5-hydroxy-7,4’-dimethoxyflavone was a competitive inhibitor and decreases the enzyme catalysis. Meanwhile β-sitosterol was a non- competitive inhibitor since the latter was bind allosterically toward enzyme. <p> Conclusion: This finding is significant for further investigation of bioactive compounds from A. subintegra on animal study.