Green Synthesis of Silver Nanoparticles using Datura metel Flower Extract Assisted by Ultrasound Method and Its Antibacterial Activity
Background: Green synthesis method of nanoparticles has been developed for several years. Besides providing an environmental-friendly process, the green synthesis of nanoparticles using plant extract provides a synergistic effect of the secondary metabolite. In recent times, the study involving the intensification process in nanoparticle formation also attracts great attention. This research deals with the green synthesis of silver nanoparticles using Datura metel flower extract as an antibacterial agent. The use of an ultrasound-assisted method for the synthesis is also investigated in this study. Methods: Synthesis of silver nanoparticles (AgNPs) using Datura metel flower extract under ultrasound-assisted method has been conducted. Evaluation of the successful synthesis was done using UV-visible spectrophotometry, particle size analyzer, x-ray diffraction, and transmission electron microscopy. The prepared AgNPs were tested as antibacterial against S. aureus, K. pneumoniae, S. pyogenes, and E. coli. Results: The ultrasound-assisted synthesis of AgNPs produces particles ranging from 25-70 nm in size; meanwhile, the reflux method demonstrated the size of 50-170 nm. These particle sizes represent the effect of the antibacterial activity as the ultrasound-assisted synthesized Ag NPs have a higher inhibition zone towards all tested bacteria. Subsequently, these data presented the applicability of Ag NPs synthesis using an ultrasound method as a potential candidate for biomedical applications. Conclusion: The profile of UV-Visible spectra and particle size analyses demonstrated the applicability of the ultrasound technique to produce a smaller size of the nanoparticles with higher antibacterial activity.