THE INTENSITY OF OXIDATIVE MODIFICATION OF PROTEINS AND PEROXIDATION OF LIPIDS IN THE BLOOD SERUM, BRAIN, LIVER AND PANCREAS TISSUES OF RATS WITH INSULIN RESISTANCE AND CONGENITAL IODINE DEFICIENCY

2019 ◽  
pp. 115-120
Author(s):  
V. B. Stetsevyat ◽  
N. M. Voronych-Semchenko
Keyword(s):  
Insulin Resistance ◽  
Iodine Deficiency ◽  
Thiobarbituric Acid ◽  
Oxidative Modification ◽  
Peroxidation Of Lipids ◽  
Diene Conjugates ◽  
Oxidative Modification Of Proteins ◽  
High Fructose ◽  
Liver And Pancreas ◽  
Dependent Processes

The results of the study about the nature of oxygen-dependent processes in rats that were on a high-fructose diet for 8 weeks under conditions of adequate iodine supply and congenital iodine deprivation is presented in the article. The significant activation of peroxide oxidation of proteins (by increasing the aldo- and keto-derivates of a neutral nature) and lipids (increased of diene conjugates content and products, that are responsible to thiobarbituric acid) of animals with insulin resistance was found. An aggravating factor in the course of these processes is a congenital iodine deficiency. The changes of oxidative modification of proteins in animals with congenital iodine deficiency had multidirectional character, and processes of lipid peroxidation mainly increased. The most pronounced changes of the studied processes were observed in the liver and pancreas of animals with insulin resistance against the background of congenital iodine deficiency.

THE INTENSITY OF OXIDATIVE MODIFICATION OF PROTEINS AND PEROXIDATION OF LIPIDS IN THE BLOOD SERUM, BRAIN, LIVER AND PANCREAS TISSUES OF RATS WITH INSULIN RESISTANCE AND CONGENITAL IODINE DEFICIENCY

2019 ◽  
pp. 115-120
Author(s):  
V. B. Stetsevyat ◽  
N. M. Voronych-Semchenko
Keyword(s):  
Insulin Resistance ◽  
Iodine Deficiency ◽  
Thiobarbituric Acid ◽  
Oxidative Modification ◽  
Peroxidation Of Lipids ◽  
Diene Conjugates ◽  
Oxidative Modification Of Proteins ◽  
High Fructose ◽  
Liver And Pancreas ◽  
Dependent Processes

The results of the study about the nature of oxygen-dependent processes in rats that were on a high-fructose diet for 8 weeks under conditions of adequate iodine supply and congenital iodine deprivation is presented in the article. The significant activation of peroxide oxidation of proteins (by increasing the aldo- and keto-derivates of a neutral nature) and lipids (increased of diene conjugates content and products, that are responsible to thiobarbituric acid) of animals with insulin resistance was found. An aggravating factor in the course of these processes is a congenital iodine deficiency. The changes of oxidative modification of proteins in animals with congenital iodine deficiency had multidirectional character, and processes of lipid peroxidation mainly increased. The most pronounced changes of the studied processes were observed in the liver and pancreas of animals with insulin resistance against the background of congenital iodine deficiency.

scholarly journals Gender Peculiarities of Protein and Lipid Peroxidation of the Oral Liquid Under the Conditions of Preclinical Iodine and Iron Deficiency

2021 ◽  
Vol 27 (1) ◽  
Author(s):  
Olga Zayats
Keyword(s):  
Lipid Peroxidation ◽  
Iron Deficiency ◽  
Oral Fluid ◽  
Thiobarbituric Acid ◽  
Oxidative Modification ◽  
Peroxidation Of Lipids ◽  
Diene Conjugates ◽  
Endogenous Factors ◽  
Dental Pathology ◽  
Oral Liquid

The prevalence of somatic pathology in children increases significantly with each passing year, which is associated with socio-economic, environmental conditions, the complex interaction of exogenous and endogenous factors. This problem may be related to micronutrient imbalances, among which iodine deficiency and sideropenia are quite common. Objective. The aim of the study is to learn the peculiarities of peroxidation of proteins and lipids, the system of antioxidant defence of oral fluid in conditions of preclinical iodine and iron deficiency. Materials and Methods. The study included 115 children aged 6-18 years. The level of peroxidation of proteins was characterized by the content of oxidative modification of protein products (aldehyde derivatives of the main nature) in the oral fluid. Peroxidation of lipids of oral fluid was assessed by the accumulation of diene conjugates (DCs) of polyunsaturated fatty acids and products responding to thiobarbituric acid (TBA-RP). Results. It has been determined that in case of iodine and iron deficiencies, their combination causes significant activation of protein and lipid peroxidation processes in the oral fluid of children, accompanied by an imbalance of the prooxidant-antioxidant system of the oral cavity. Conclusion. Such changes can be predictors of the development of dental pathology

scholarly journals Peculiarities of Metabolism of Rats with Fructose-induced Insulin Resistance Against the Background of Congenital and Acquired Iodine Deficiency

2019 ◽  
Vol 26 (4) ◽  
Author(s):  
Vasyl Stetseviat
Keyword(s):  
Insulin Resistance ◽  
Carbohydrate Metabolism ◽  
Iodine Deficiency ◽  
Total Cholesterol Level ◽  
Glycosylated Hemoglobin ◽  
High Density Lipoproteins ◽  
High Fructose Diet ◽  
High Fructose ◽  
Increased Risk

Animals following a high-fructose diet during eight weeks, have experienced changes in metabolism and the signs of insulin resistance have developed. Under such conditions, moderate hyperglycemia, hyperinsulinemia, hyperuricemia, an increase of the level of glycosylated hemoglobin in whole blood were observed. The significant role of the HOMA-IR index, as an early marker of carbohydrate metabolism disorders at the stage of pre-diabetes, has been confirmed. In experimental animals against the background of the high-fructose diet, the changes in the lipid spectrum of the blood were revealed: an increase of the total cholesterol level, low-density lipoproteins, triglycerides against the background of a high-density lipoproteins decrease. These disorders and a significant increase of the atherogenicity reflect the development of secondary dyslipidemia. In this case, the disorders of carbohydrate metabolism were combined with the degree of dyslipidemia. Males were found to have at increased risk of development the insulin resistance and comorbid pathology. Iodine deficiency, especially of congenital nature, is an aggravating factor of metabolic disorders. The obtained data can serve as a basis for extend of preventive measures and identification of the priority treatment schemes for type 2 diabetes mellitus in residents of endemic regions.

scholarly journals Застосування ентеросорбенту “карболайн” для корекції окиснювальних процесів у щурів різного віку, уражених натрію нітритом на тлі тютюнової інтоксикації

2017 ◽  
Author(s):  
P. H. Lykhatskyi ◽  
L. S. Fira
Keyword(s):  
Sodium Nitrite ◽  
Thiobarbituric Acid ◽  
Oxidative Modification ◽  
Comprehensive Treatment ◽  
Oxidative Modification Of Proteins ◽  
Different Ages ◽  
Extreme Factors ◽  
Old Rats ◽  
Oxidative Processes ◽  
Radical Processes

Introduction. One of the priorities of toxicology and medicine is the study of the characteristics and mechanisms of action of combined xenobiotics – many risk factors for environmentally dependent diseases. Under the influence of extreme factors of various origins (heavy metals, nitrates, pesticides, tobacco, alcohol), formation of ROS, leading to the development of oxidative stress in the organism, which is accompanied by activation of lipid peroxidation and oxidative modification of proteins is enhanced. Under these conditions antioxidants and sorbents are commonly used.The aim of the study – to explore efficiency of enterosorbent karbolayn and its effect on oxidative processes in rats of different ages affected by sodium nitrite on the background of the 30-day tobacco intoxication.Research Methods. Given the defeat of rats of different ages sodium nitrite on the background of tobacco intoxication were tested for the ROS in the population of neutrophils, obtained by centrifugation on a double density gradient 1.077 and 1.093 ficoll-verografin, the content of TBA-active products in reaction with thiobarbituric acid and oxidative modification of proteins 2.4 dynitrofenilhidrazones content.Results and Discussion. The highest activity of free radical processes was marked after the defeat of sodium nitrite in 72 hours to 30 day of suspension toxicity of tobacco smoke. Immature and old rats were much more sensitive to the action of toxins than mature animals. Immature and old rats were much more sensitive to the action of toxins than mature animals. In order to inhibition of oxidative processes activated sorbent used by us karbolayn led to a slight normalization of the studied parameters. There was a trend toward reduction of reactive oxygen species in blood, TBA-active products and 2.4 dynitrophenilhidrazones in the studied tissues.Conclusions. The results indicate the advisability of including karbolayn to comprehensive treatment of poisoning of various origins.

scholarly journals Study of prooxidant activity and some indicators of the pituitary-gonadal system in patients suffering from chronic endometritis

2020 ◽  
Vol 22 (1) ◽  
pp. 66-69
Author(s):  
N V Kirillova ◽  
O M Spasenkova ◽  
A G Platonova
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Protein Modification ◽  
Aliphatic Aldehyde ◽  
Oxidative Modification ◽  
Control Group ◽  
Diene Conjugates ◽  
Oxidative Modification Of Proteins ◽  
Chronic Endometritis ◽  
Oxidative Protein Modification

The state of lipid peroxidation and the oxidative modification of proteins in the blood of 29 women with chronic endometritis aged 27 to 40 years were studied. The control group consisted of 19 healthy women aged 27 to 44 years. The intensity of lipid peroxidation was estimated by the level of diene conjugates and malondialdehyde, the oxidative modification of proteins - by the level of carbonyl derivatives. It was shown that in the blood of women with chronic endometritis, the concentration of aliphatic aldehyde groups and 1,9 times the carbonyl groups in protein molecules increases by 3,3 times compared with the control group. A significant increase in diene conjugates by 34% and malondialdehyde in blood plasma and red blood cells by 47 and 43%, respectively, was established. The increase in lipid peroxidation products and oxidative protein modification in the blood indicates the development of oxidative stress in women suffering from chronic endometritis. Compared with healthy patients with chronic endometritis, a convincing tendency toward an increase in prolactin and anti- Muller hormone against a decrease in progesterone was revealed in the group compared to healthy ones. To predict possible complications in patients with chronic endometritis, it is advisable to study the indicators of protein and lipid peroxidation as markers of oxidative stress and the severity of the inflammatory process. In addition, such patients should be advised to take antioxidant drugs in combination with the basic therapy.

Modern aspects of professional cancer treatment: prospects for the use of medical ozone in the correction of free radical homeostasis

2020 ◽  
Vol 60 (11) ◽  
pp. 767-770
Author(s):  
Evgeniya S. Zhukova ◽  
Anna Yu. Kashina ◽  
Anastasiya M. Irkaeva
Keyword(s):  
Free Radical ◽  
Thiobarbituric Acid ◽  
Oxidative Modification ◽  
Ozone Exposure ◽  
Oxygen Mixture ◽  
Antitumor Therapy ◽  
Oxidative Modification Of Proteins ◽  
Tumor Tissues ◽  
Comprehensive Health ◽  
Medical Ozone

Introduction. The well-known features of oncogenesis make it possible to assume the prospects of medical ozone as part of a comprehensive health-saving therapy for professional oncopathologies. Due to the risk of stimulating the proliferation of tumor cells, research on the search for optimal modes of ozone exposure is relevant. The aim of the study was to evaluate the possibility of using ozonated saline solution (OSS) as part of complex antitumor therapy in an experiment. Materials and methods. The effect of OSS with different concentrations of ozone in the ozone-oxygen mixture on the content of hemoglobin, active products of thiobarbituric acid (TBA) of lipid peroxidation (LP), the degree of oxidative modification of proteins, and the activity of superoxide dismutase and catalase in blood, liver, spleen, and tumor tissues was studied in Mature rats with normal and transplanted cholangiocellular carcinoma. Results. The course effect of the OSS did not have statistically significant changes in the studied biochemical parameters under the conditions of the physiological norm in the experiment. Exposure to OSS during oncogenesis led to a decrease in the content of TBA-active LP products in the liver by more than 30%. Conclusions. Course exposure to OSS with an ozone concentration in an ozone-oxygen mixture of 400 micrograms/l may be promising for the correction of free radical homeostasis as part of complex antitumor therapy, including in oncogenesis due to occupational factors.

scholarly journals Some biochemical parameters of the synovial liquid for estimation of effectiveness of the treatment of the knee joint osteoarthrosis

2012 ◽  
Vol 58 (2) ◽  
pp. 230-236 ◽  
Author(s):  
M.G. Meshcheryakova ◽  
Ya.G. Trilis ◽  
N.V. Kirillova ◽  
A.T. Alpatova ◽  
I.A. Muhin ◽  
...  
Keyword(s):  
Knee Joint ◽  
Biochemical Parameters ◽  
Specific Treatment ◽  
Thiobarbituric Acid ◽  
Oxidative Modification ◽  
Total Protein Content ◽  
Laboratory Diagnostics ◽  
Carbonyl Groups ◽  
Thiobarbituric Acid Reactive Substances ◽  
Oxidative Modification Of Proteins

The knee joint osteoarthrosis is accompanied by activation of the oxidative stress in the synovial liquid. Specific treatment decreased or even normalized such biochemical parameters of the synovial liquid as the carbonyl groups, thiobarbituric acid reactive substances (TBARS) and total protein content. The most demonstrative changes were found for early and late markers of the oxidative modification of proteins. These parameters may be used in laboratory diagnostics of the depth of the degenerative-dystrophic process in the knee joint and for the estimation of the effectiveness of the treatment.

Non-Alcoholic Fatty Liver Disease in Overweight Children and its Relationship with Retinol Serum Levels

2008 ◽  
Vol 78 (1) ◽  
pp. 27-32 ◽  
Author(s):  
Suano de Souza ◽  
Silverio Amancio ◽  
Saccardo Sarni ◽  
Sacchi Pitta ◽  
Fernandes ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Liver Disease ◽  
Fatty Liver ◽  
Lipid Profile ◽  
Fatty Liver Disease ◽  
Thiobarbituric Acid ◽  
Serum Levels ◽  
Control Group ◽  
Alcoholic Fatty Liver ◽  
Alcoholic Fatty Liver Disease

Objectives: To evaluate the frequency of non-alcoholic fatty liver disease, the retinol serum levels, lipid profile, and insulin resistance in overweight/obese children. To relate these biochemical variables with the risk of this disease in the population studied. Methods: The study was cross-sectional and prospective, with 46 overweight/obese school children (28 female, 18 male; mean age 8.6 years). The control group consisted of 45 children, paired by age and gender. Hepatic steatosis, evaluated by ultrasound, was classified as normal, mild, moderate, or severe. Also evaluated were serum retinol levels; thiobarbituric acid reactive substances; lipid profile; and fasting glucose and serum insulin levels, used for the calculation of the Homeostasis Model Assessment. Results: Hepatic ultrasound alterations were found in 56.5% and 48,9% of the overweight/obese and control group children, respectively. Presence of obesity was associated with high levels of triglycerides (OR = 4.6; P = 0.002). In the studied children, the risk of steatosis was related to a trend to a higher percentage of retinol inadequacy (OR = 2.8; p = 0.051); there was no association with thiobarbituric acid reactive substances, lipid profile, or insulin resistance. Conclusions: The high frequency of non-alcoholic fatty liver disease in both groups, evaluated by hepatic ultrasound, in low-socioeconomic level children, independent of nutritional condition and without significant association with insulin resistance, emphasizes that especially in developing countries, other risk factors such as micronutrient deficiencies (e.g. vitamin A) are involved.

The evaluation of lipid peroxidation and oxidative modification of proteins in blood serum under obesity development and the consumption of aqueous kidney beans Phaseolus vulgaris pods extract

2020 ◽  
Vol 33 (1) ◽  
pp. 38-44
Author(s):  
Alona Yurchenko ◽  
Daryna Krenytska ◽  
Olexii Savchuk ◽  
Tetiana Halenova ◽  
Natalia Raksha ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Oxidative Modification ◽  
Resistance Development ◽  
Oxidative Modification Of Proteins ◽  
Kidney Beans ◽  
Calorie Diet ◽  
Diet Control ◽  
High Calorie Diet ◽  
High Calorie

AbstractOur interest has focused on the investigation of the anti-obese potential of kidney beans (P. vulgaris) pods extract. In the course of the study, obesity development in rats was induced with high-calorie diet. Control and obese rats then have consumed with aqueous kidney beans (P. vulgaris) pods extract during 6 weeks (200 mg/kg). Results show that the long-term consumption of P. vulgaris pods extract can lead to the reduction of hyperglycemia and insulin resistance development. Furthermore, we saw a normalization of lipid peroxidation parameters and oxidative modification of protein due to the consumption of the kidney beans (P. vulgaris) pods extract. Our experimental data demonstrate the ability of the kidney beans (P. vulgaris) pod extracts to mitigate obesity development but the details of this mechanism remains to be not fully understood.

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