scholarly journals Evaluation of Antifungal Activity of Various Plant Latex against Selected Fungal Strains

Author(s):  
Mubashira Begum

Abstract: Latex is a natural plant polymer and milky white fluid distributed throughout the plant body like leaves, stems, roots and fruits of all flowering plants and consists of proteins, alkaloids, starches, sugars, oils, tannins, resins and gums that coagulate on-air exposure. It is secreted by specialized plant cells called Laticifers. Plants exude latex in response to physical damage. The present study was carried out to assess the potential antifungal activity of latex of five different plants namely Plumeria rubra, Plumeria alba, Aloe vera, Calotropis procera and Calotropis gigantea against three different pathogenic strains of fungi. All five latexes were found to show good to moderated activity against all the three fungal strains, namely Trichoderma viride, Aspergillus flavus and Aspergillus niger. The latex of each plant was tested in two volumes (i.e. 10µl & 20µl), and it was found that the antifungal activity was volume-dependent, and a significant difference was also observed in the case of different fungal strains. The antifungal activity of plant latexes was evaluated by Agar well diffusion method; results revealed that among all the five latexes, the fresh latex of Plumeria rubra (Vol: 10µl &20µl) showed excellent antifungal activity against Aspergillus niger and Aspergillus flavus, whereas fresh latex of Aloe vera (Vol: 10µl & 20µl) showed excellent antifungal activity against Trichoderma viride. The results of the current research imply that the antifungal activity of latex varies with the species of plants and the fungal strains used. The results therefore demonstrated that, the used five latexes effectively inhibited the growth of three tested fungi. Hence, these plant latexes are natural, ecofriendly and can be used as good candidates for the treatment of various fungal diseases. In further words, they can be used for therapy of antifungal-resistant fungi. Keywords: Antifungal activity, Proteins, Alkaloids, Tannins, Resins, Laticifers, Plumeria rubra, Plumeria alba, Aloe vera, Calotropis procera, Calotropis gigantea, Aspergillus flavus, Aspergillus niger, Trichoderma viride, Latex, Agar well diffusion.

Author(s):  
Berivan A. Abdullah ◽  
Diana T. Salih ◽  
Asia A. M. Saadullah

Introduction: Fungi live everywhere in the environment, most of them are not dangerous, but some types of fungi can be harmful to humanhealth. The medicinal plants contain many antimicrobial components that make them recently used as powerful drugs. The aim of the present investigation was to examine the antifungal potential and minimum inhibitory concentration (MIC) of three plant extract: Aloe vera gel, cinnamon (Cinnamomum zeylanicum) and turmeric (Curcuma longa) against three fungal species: Aspergillus niger, Candida albicans and Fusarium oxysporum. Materials and Methods: The plant materials were extracted using solvents DMSO and ethanol and then were tested against the selected fungal isolates using well diffusion method. Results and Discussion: Antifungal activity of Aloe vera against Aspergillus niger showed MIC value of 25% whereas for Candida albicans and Fusarium oxysporum the MIC obtained was 100%. Both cinnamon and turmeric showed maximum potency against Aspergillus niger, Candida albicans and Fusarium oxysporum at highest MIC value of 100 %. The degree of inhibition increased correspondingly with increasing concentrations of the plant extracts. Conclusions: the tested plant extracts have an antifungal activity and could be used as alternative drugs.


2010 ◽  
Vol 12 (4) ◽  
pp. 53-57 ◽  
Author(s):  
Agata Markowska-Szczupak ◽  
Krzysztof Ulfig ◽  
Barbara Grzmil ◽  
Antoni Morawski

A preliminary study on antifungal effect of TiO2-based paints in natural indoor light The antifungal activity of four commercial photocatalytic paints (KEIM Ecosil ME, Titanium FA, Photo Silicate and Silicate D) in natural indoor light was investigated. The paints contained TiO2 in rutile and anatase crystalline forms as evidenced by means of the X-ray diffraction analysis. In most cases the paints inhibited growth of fungi viz. Trichoderma viride, Aspergillus niger, Coonemeria crustacea, Eurotium herbariorum, and Dactylomyces sp. The KEIM Ecosil ME paint displayed the highest antifungal effect in the light, which could be explained with the highest anatase content. The paint antifungal activity and the fungal sensitivity to the TiO2-mediated photocatalytic reaction both decreased in the following orders: KEIM Ecosil ME > Titanium FA > Photo Silicate > Silicate D and T. viride > Dactylomyces sp. > A. niger > E. herbariorum.


Author(s):  
P Danish ◽  
Q Ali ◽  
MM Hafeez ◽  
A Malik

Aloe vera is a well-known medicinal plant used in many therapeutic purposes. Naturally it is composed of many useful compounds that have ability to use for treatment of many diseases. The active compounds reported in this plant are saponins, sugar, enzymes, vitamins, aloesin, aloeemodin, aloin, acemannan aloemannan, aloeride, methylchromones, flavonoids, naftoquinones, sterols, minerals, anthraquinones, amino acids, lignin and salicylic acid and other different compounds including fat-soluble and water-soluble vitamins, enzymes, minerals, simple/complex sugars, organic acid and phenolic compounds. In this study aloe vera is used for antibacterial and antifulgal activity against different strains of bacteria and pathogenic fungal strains. Ethanol extract of Aloe vera leaves and roots is applied on these bacterial and fungal strains in different concentrations (15, 20, 25, 30µl). Bacillus cereus, Bacillus subtitis, Bacillus megaterium, Streptococcus pyogenes, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Acinetobacter baumannii, and some other bacterial strains are used for this study. Escherichia coli and Agrobacterium tumefacins shows zone of inhibition around 18mm which consider as good result. Bacillus subtitis and Bacillus megaterium also shows good result around 16mm. Proteus mirabilis and Pseudomonas aeruginosa shows minimum zone of inhibition which is around 11mm. among all used fungal strains (fuserium oxysporum, Candida albicans, Aspergillus fumigatus, Aspergillus niger) fuserium oxysporum and Aspergillus niger shows excellent results around 19mm both against root extract and leaves extract.


Nanoscale ◽  
2018 ◽  
Vol 10 (44) ◽  
pp. 20702-20716 ◽  
Author(s):  
Clemencia Chaves-Lopez ◽  
Hang N. Nguyen ◽  
Rodrigo C. Oliveira ◽  
Enrico T. Nadres ◽  
Antonello Paparella ◽  
...  

The present study compares for the first time the effects of h-MoO3and α-MoO3against two fungal strains:Aspergillus nigerandAspergillus flavus.


2020 ◽  
Vol 7 (17) ◽  
pp. 327-337
Author(s):  
U. N. Emiri ◽  
E. B. Enaregha

The study investigated fungi associated with post harvest tomato fruits sold in the open market in Port Harcourt Metropolis. The antifungal activity (bitter leaf) extracts against spoilage was studied using well-in-agar diffusion method. Results showed that mean percentage incidence of fungi isolated from tomato fruits were Rhizopus stolonifer (56%), Aspergillus niger (62%) and Altermaria altermata (35%). Aqueous and ethanolic extracts of Vernonia amygdalina at different concentrations (20%, 40%, and 60%) (w/v) were used against fungi isolates, while water and ketoconzole (0.5 mg/mL) served as negative and positive control, respectively. Aqueous and ethanolic extracts of V. amygdalina inhibited the growth of all three test fungi. There were significant differences (p > 0.05) in the mean inhibitory effects of plant extracts and control. Antifungal activity measured as diameter of zone of inhibition revealed that V. amygdalina acqueous extract at 60% was very active against Aspergillus niger (16.50 mm) and Alternaria altermata (16.00 mm), while being moderately active against Rhizopus stolonifer (13.00 mm). However, 60% ethanolic extract of V. amygdalina was very active against A. niger (19.00 mm), A. altermata (17.00 mm) and Rhizopus stolonifer (15.80 mm). Phytochemical screening of V. amygdalina revealed the presence of tannins, oxalate, saponnins, flavanoid, cynogenic glucoside, phytate and Alkaloids V. amygdalina could serve as a potentially viable alternative to chemical fungicides in the preservation of post harvest tomato fruits (Lycopersicum esculentum).


2010 ◽  
Vol 9 (2) ◽  
pp. 60
Author(s):  
J.S. Al-Hussaini, and A. M. G. Al-Mohana

The aim of this study was to evaluate the antifungal activity of the ethanolic extract of three local plants ( Elettaria cardamomum, Aloe vera, Thyme Vulgaris) against the growth of pathogenic Candida albicans in culture media. The antifungal activity was carried out by using agar well diffusion method. Ethanolic extracts of Elettaria cardamomum and Aloe vera inhibited the growth of Candida albicans isolates at all concentrations which tested in the present study (25, 50, 100, 150, 200, and 400) mg/ml, while the extract of Thymus vulgaris showed no activity against tested Candida albicans


2015 ◽  
Vol 2 (1) ◽  
pp. 1-6 ◽  
Author(s):  
Apoorva Gupta ◽  
Anand K. Halve

A new series of 4-[3-chloro-2-(4-hydroxy-3-methoxybenzyllidene)-4-oxoazetidin-1-yl]amino-N-(substituted)benzenesulfonamide, 4-{3-chloro-2-[5-methoxy-2-nitro-4-(prop-2-en-1-yloxy)benz yllidene]-4-oxoazetidin-1-yl}]amino}-N-(substituted)benzenesulfonamide and 4-{3-chloro-2-[4-hydroxy-3-methoxy-5-(prop-2-en-1-yl)benzylidene]-4-oxoazetidin-1-yl}amino}-N-(substituted) benzenesulfonamide were synthesized using appropriate synthetic route. The chemical structures of all the synthesized compounds were deduced on the basis of elemental analysis and spectroscopic data. The antifungal activity of the synthesized compounds was screened against several fungus. The synthesized compounds show potent antifungal activity against Aspergillus niger & Aspergillus flavus and significant structure-activity relationship (SAR) trends.


2012 ◽  
Vol 64 (3) ◽  
pp. 911-916 ◽  
Author(s):  
Danka Bukvicki ◽  
M. Veljic ◽  
Marina Sokovic ◽  
Slavica Grujic ◽  
P.D. Marin

The antibacterial and antifungal activity of methanol extracts of the genuine mosses Abietinella abietina, Neckera crispa, Platyhypnidium riparoides, Cratoneuron filicinum var. filicinum and Campylium protensum were evaluated. Antibacterial activity was tested against Gram (+) Staphylococcus aureus, Micrococcus flavus, Bacillus cereus and Gram (-) bacteria Escherichia coli and Salmonella typhimurium. Antifungal activity was tested using micromycetes Trichoderma viride, Penicillium funiculosum, Penicillium ochrochloron, Aspergillus flavus, A. niger and A. fumigatus. The methanol extracts of all moss species showed an antimicrobial effect against the tested microorganisms. Significant antibacterial effect was achieved for Cratoneuron filicinum and Neckera crispa. The most sensitive bacteria were Bacillus subtilis and Micrococcus flavus. Abietinella abietina and Neckera crispa showed an antifungal effect against micromycetes Trichoderma viride, Penicillium ochrachloron, P. funiculosum and Aspergillus flavus.


Author(s):  
B. Mohana ◽  
Shiva Kameshwari ◽  
M. K. Prasana Kumar

Aqueous extract of Urginea indica kunth. (Udupi acccession) was screened for antifungal activity against Fusarium oxysporum, Sclerotium rolfsii, Magnaporthe orzae, Aspergillus flavus, Alternaria alternata, Aspergillus niger and Fusarium moniliformae by poisoned food technique. The results confirmed Urginea indica extracts showed very significant antifungal activity against Fusarium oxysporum and showed significant inhibition for Sclerotium rolfsii and Magnapothea orzaea it showed no activity against Aspergillus niger and Aspergillus flavus. All the activity was evaluated to determine the lowest concentration required to inhibit visible mycelial growth of the pathogen at minmum concentration. Fusarium oxysporum showed very significant inhibition in 10% concentration (Reconfirmed) while Sclerotium showed significant inhibition in 25% concentration followed by Magnoporthe oryzae. The number of sclerotia spores formed was also reduced drastically. These results show that a potential and safe antifungal agent can be obtained from Urginea.


2020 ◽  
Vol 150 ◽  
pp. 02003 ◽  
Author(s):  
Azeddin El Barnossi ◽  
Fatimazahrae Moussaid ◽  
Abdelilah Iraqi Housseini

Candida albicans an opportunistic pathogenic fungus causes many infections in humans. Whereas Aspergillus niger is a fungus that can produce ochratoxins, a group of extremely dangerous secondary metabolites that are classified as potentially carcinogenic to humans and also causing deterioration in grapes, strawberries, etc. The purpose of the current study is to isolate, purify, identify and characterize new microorganisms associated with solid green household waste for the control of C. albicans and A. niger. The antifungal activity of bacterial isolates was carried out in vitro by the agar plug diffusion method, the disk and well diffusion method. The isolate that showed promising activity has been identified by those macroscopic, microscopic and biochemical characteristics. The results obtained in the course of this study showed the isolation of an isolate named Gn-A11-18, which was shown to have significant inhibitory activity with a 42.66% inhibition percentage against A. niger and a 44.66 mm inhibition diameter against C. albicans compared to the controls. The identification of Gn-A11-18 isolate has shown that this isolate belongs to the genus Bacillus with a similarity to Bacillus subtilis and Bacillus tequilensis. In the light of the results of this study, we can suggest that the bioactive compound of Bacillus sp. Gn-A11-18 could become a biological alternate that could have an important role to fight against C. albicans and A. niger.


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