scholarly journals IL-4 Level in Rifampicin-Sensitive and Rifampicin-Resistant Lung Tuberculosis Patients

2020 ◽  
Vol 27 (1) ◽  
Author(s):  
Joko Susanto ◽  
Jusak Nugraha ◽  
Soedarsono Soedarsono
Keyword(s):  
Pulmonary Tuberculosis ◽  
Operating Characteristics ◽  
Mycobacterium Tuberculosis Infection ◽  
Cross Sectional ◽  
Tuberculosis Patients ◽  
Lung Tuberculosis ◽  
Characteristics Analysis ◽  
Resistant Group ◽  
The Difference ◽  
Sensitive Group

Tuberculosis remains a global health burden. Mycobacterium tuberculosis infection causes humoral and cellularresponses. Macrophages of patients with pulmonary tuberculosis evolve M1 polarization that blocks infection orimmunosuppressive M2, promoting tissue repair mediated by IL-4, IL-10, and IL-13. Previous research showed a decrease ofIL-4R and IL-10 expression in lung macrophages of anti-TB drug resistance. A molecular test can detectrifampicin- resistance. There has been no study, which showed the difference in serum IL-4 levels in rifampicin-sensitive andrifampicin-resistant tuberculosis patients. This study aimed to determine the difference between circulating IL-4 levels inrifampicin-sensitive and rifampicin-resistant pulmonary tuberculosis patients. This cross-sectional observational studyconsecutively recruited subjects based on positive molecular and acid-fast bacilli microscopic examination from MDR-TBClinic of the Dr. Soetomo Hospital between December 2018 to March 2019. Subjects were classified into arifampicin-sensitive and rifampicin-resistant group. On ELISA measurement, IL-4 data were analyzed with SPSS version 17.Mann-Whitney U test and ROC analysis tests were performed, and p < 0.05 was significant for α=0.05 (95% CI). There wassignificant difference between rifampicin-sensitive group (420±281 pg/mL) and rifampicin-resistant group(253±279 pg/mL) (p=0.014). Receiver operating characteristics analysis showed AUC 0.70, the sensitivity of 81.5%, thespecificity of 63.6%, and the cut-off value of 235.6 pg/mL. There was a significantly higher level of circulating IL-4 in therifampicin-sensitive group than the rifampicin-resistant group. IL-4 level in healthy subjects should be measured as thenormal value in the population. Immunology and metabolic parameters should be performed to increase samplehomogeneity. Further study was also needed to understand the IL-4 role in rifampicin resistance of lung tuberculosispatients in the Indonesia population.

scholarly journals Determinan Kepatuhan Minum Obat Pasien Tuberkulosis Paru

2019 ◽  
Vol 1 (2) ◽  
pp. 176-185
Author(s):  
Christine Vita Gloria ◽  
Zulmeliza Rasyid ◽  
Sherly Vermita W ◽  
Elmia Kursani ◽  
Bizanti Umayyah
Keyword(s):  
Pulmonary Tuberculosis ◽  
Health Center ◽  
Family Support ◽  
Health Workers ◽  
Treatment Compliance ◽  
P Value ◽  
Cross Sectional ◽  
Tuberculosis Patients ◽  
Lung Tuberculosis

This study aims to see whether the determinants are related to treatment compliance for pulmonary tuberculosis patients at the 2019 health center. This type of research is a quantitative observational cross-sectional design. The results showed that with a P value of 0.05, there was a significant relationship between knowledge (P value = 0.005 and POR = 14.276), attitudes (P value = 0.506 and POR = 1.615), family support (P value = 0.038 and POR = 1,961), the role of health workers (P values = 0.026 and POR = 4.440), with medication adherence for pulmonary tuberculosis patients. Conclusions, of the 4 variables there are 3 variables related to adherence to taking pulmonary TB drugs in the Work Area of the Siak Hulu II Health Center, namely knowledge, family support, and the role of health workers and 1 unrelated variable, attitude.   Keywords: Obedience, Medicine, Lung Tuberculosis

scholarly journals How Much Depressed are Lung Tuberculosis Patients in Tangerang, Banten Province, Indonesia?

Medicinus ◽  
2020 ◽  
Vol 7 (6) ◽  
pp. 195
Author(s):  
Tirta Darmawan ◽  
Engelberta Pardamean ◽  
Vinsensa Anabella ◽  
Mark Mark ◽  
Amelia Gianina Widjaja
Keyword(s):  
Pulmonary Tuberculosis ◽  
Depression Scale ◽  
Severe Depression ◽  
Mild Depression ◽  
Cross Sectional ◽  
Tuberculosis Patients ◽  
Lung Tuberculosis ◽  
Low Educated ◽  
Almost All ◽  
Patients Experience

<p><strong>Introduction : </strong>Tuberculosis is an infectious disease caused by Mycobacterium Tuberculosis (MTB) and mostly attacks the lung and other organs. In patients with pulmonary tuberculosis, depression can occur and the factors that can cause it are age, sex, marital status, level of education, emplyment, long suffering from pulmonary tuberculosis and comorbidities. The purpose of this study is to show the prevalence and also degree of depression in patients with lung Tuberculosis in Tangerang, Banten Province, Indonesia.</p><p><strong>Methods : </strong>The study design is cross sectional by asking participants to fill Zung Self-Rating Depression Scale that have been translated to Indonesian language. Participants were 129 patients with lung tuberculosis in Siloam General Hospitals Lippo Karawaci, Puskesmas Kutai and Puskesmas Curug.</p><p><strong>Results : </strong>Mean age of the 137 patients with lung tuberculosis is 47.73 <span style="text-decoration: underline;">+</span> 8.50 years old, and the mean of their height and weight are 163.94<span style="text-decoration: underline;">+</span> 7.59 centimeters and 51.10 <span style="text-decoration: underline;">+</span> 7.40 kilograms. More than a half of the patients are male (69%), almost a half of the patients are low educated (48%) and almost all of the patients have been married (93.8%). More than a half of the lung tuberculosis patients experience deppression (70,5%). Approximately a half of the lung tuberculosis patients are having mild depression (50.4%). There is 1 patient is having severe depression (0.8%).</p><p><strong>Conclusion : </strong>Degree of depression in adult patients suffer from lung Tuberculosis in Tangerang, Banten Province, Indonesia is high. More than a half of adult lung Tuberculosis patients are having depression. Depression is one of problems that should be concerned and also treated in patients with lung tuberculosis. Psychiatrist involvement in managing patients with lung tuberculosis is needed.</p>

scholarly journals Role of atd Angle of Palm in Diagnosis of Pulmonary Tuberculosis

2017 ◽  
Vol 8 (2) ◽  
pp. 85-88
Author(s):  
Afrose Ahmed ◽  
Shamim Ara ◽  
KM Monjurul Alom ◽  
Saida Rashed ◽  
Sadia Rahman ◽  
...  
Keyword(s):  
Pulmonary Tuberculosis ◽  
Interleukin 1 ◽  
High Risk Population ◽  
Cross Sectional ◽  
Tuberculosis Patients ◽  
Medical College ◽  
Non Invasive ◽  
Control Subjects ◽  
Mannose Binding Protein ◽  
The Difference

Background: Dermtoglyphics are the lines and ridges forming a skin pattern, especially on the palm of the hands, fingertips and soles of feet. Differentiations of dermal ridges in early life are genetically determined1. Any disturbance by genetic factors causes development of unusual dermatoglyphics. On the other hand, deficiency of Mannose binding protein (MBP) gene and Interleukin-1 (IL-1) gene cause spread of pulmonary tuberculosis2. The dermatoglyphic pattern in patient with pulmonary tuberculosis can represent an anatomical, non-invasive, inexpensive tool for screening high-risk population, and facilitates early prediction of vulnerable population of PTB.Materials and Methods: A Cross-sectional, analytical type of study was conducted in Department of Anatomy, Dhaka Medical College, Dhaka, from January 2012 to December 2012. The study was performed in 100 patients suffering from pulmonary tuberculosis and 100 healthy individuals.Results: There was significant reduction in mean±SD of atd angle in pulmonary tuberculosis patients in comparison to control subjects. Mean±SD of atd angle was 37.87±3.403 in pulmonary tuberculosis patients and 49.77±4.628 in the control subjects. The difference was highly significant (P<0.001).Conclusion: The atd angle was found significantly narrower in the study group compared to the control subjects.Anwer Khan Modern Medical College Journal Vol. 8, No. 2: Jul 2017, P 85-88

scholarly journals THE DIFFERENCE OF MAP1LC3 LEVEL AS MACROPHAGE AUTOPHAGY MARKER BETWEEN RESISTANT AND SENSITIVE TUBERCULOSIS PATIENTS ON RIFAMPICIN

2018 ◽  
Vol 7 (1) ◽  
pp. 6
Author(s):  
Dian Novita Wulandari ◽  
Jusak Nugraha ◽  
Soedarsono Soedarsono
Keyword(s):  
Defense Mechanisms ◽  
Serum Levels ◽  
Immune Defense ◽  
Cross Sectional ◽  
Tuberculosis Patients ◽  
Macrophage Cells ◽  
Mdr Tb ◽  
Associated Proteins ◽  
Resistant Group ◽  
The Difference

M. tuberculosis (MTB) is an intracelular bacteria that live in the host macrophage cells. Several organs can be affected by tuberculosis but most major illnesses are lung diseases. Immediately after infection, MTB will be phagocytosed by the alveolar macrophage cells and can survive in the phagosome. The macrophage plays a role in innate immunity towards an infection using autophagy by removing the microbe directly via phagocytosis. When bacteria phagocytosized, vacuole membrane formed double membranes called autophagosome, and followed by degradation by lysosome, which known as  autolysosome. Induction of autophagy can be observed on the formation of microtubule-associated proteins 1B lightchain 3B (MAP1LC3B/LC3). MAP1LC3B is protein that have role at autophagic way for selection autophagy substrate and biogenesis. In this study we are used serum from patients TB with rifampicin resistant and rifampicin sensitive as control. Samples were divided using gene expert to differentiate between resistant and sensitive rifampicin.This research aims to compare MAP1LC3B levels in resistant and sensitive rifampicin to study macrophages respond in autophagic way in tuberculosis patients, and give information for define therapy plan to improve therapy for MDR-TB patients. Type of this research is a case control study design with cross sectional research with each groups sample is 19 from age 18-65 years old. Result, MAP1LC3B serum levels on the rifampicin resistant group are lower compared to rifampicin sensitive group. This occur because MTB is able to hide and evade innate immune defense mechanisms. MTB can maintain intracellular growth inside the phagosome by inhibiting phagolysosome formation in autophagy process especially inhibit MAP1LC3B formation by PDIM. 

scholarly journals The Effect of Side Effects Tuberculosis Treatment in the Early Stage Towards Compliance with Tuberculosis Patients

2021 ◽  
Vol 5 (01) ◽  
pp. 29-32
Author(s):  
Joko Sapto Pramono ◽  
Nilam Noorma ◽  
Andi Lis Arming Gandini ◽  
Sopia Fitriani
Keyword(s):  
Side Effects ◽  
Pulmonary Tuberculosis ◽  
Health Workers ◽  
Early Stage ◽  
Treatment Compliance ◽  
Tuberculosis Treatment ◽  
Cross Sectional ◽  
Tuberculosis Patients ◽  
Early Stages ◽  
Significant Difference

Pulmonary tuberculosis treatment causes various side effects including nausea and vomiting, itching, vision problems, and anemia. Drug side effects in the early stages are one of the causes of non-adherence to complete treatment. The purpose of this study was to analyze the effect of the side effects Tuberculosis treatment in the early stages on treatment compliance for tuberculosis patients. This study used a cross sectional design. Samples were taken as many as 71 respondents, the instruments used were side effects of early-stage tuberculosis treatment and compliance with tuberculosis treatment in tuberculosis patients from the Morisky Medication Adherence Scale (MMAS). The results of the study found 97.7% adherent and 2.3% non-adherent, 39.5% mild side effects and 60.5% severe side effects. Chi-square test showed that no significant difference between the side effects of TB treatment in the early stages of tuberculosis treatment compliance in patients p = 0.669 at significant level of 95% (α = 0.05). There was no effect between the side effects of Tuberculosis treatment in the early stages of treatment compliance for Tuberculosis patients. It was recommended that health workers continue to monitor the side effects of tuberculosis treatment and provide motivation to carry out treatment completely. Keywords: side effects; early stage; treatment; pulmonary tuberculosis; compliance

scholarly journals KORELASI KADAR SERUM IFN-γ, EKSPRESI DAN FUNGSI RESEPTOR IFN-γ, DENGAN KEJADIAN TUBERKULOSIS PARU (The Correlation Of Ifn-γ Serum Level, Ifn-γ Receptor Expression And Function, With Pulmonary Tuberculosis)

2013 ◽  
Vol 3 (3) ◽  
Author(s):  
Jahja Teguh Widjaja
Keyword(s):  
Stem Cell ◽  
Serum Level ◽  
Pulmonary Tuberculosis ◽  
Mononuclear Cells ◽  
Receptor Expression ◽  
Cross Sectional ◽  
Tuberculosis Patients ◽  
Ifn Γ ◽  
Cross Sectional Design ◽  
And Function

AbstrakUntuk dapat bekerja dengan efektif menghadapi M. tuberculosis, sistim imunitas seluler memerlukan kadar IFN-γ dan reseptor IFN-γ di sel-sel mononuklear yang bekerja optimal. Tujuan penelitian ini adalah menganalisis korelasi antara kadar serum IFN-, ekspresi dan fungsi reseptor IFN-γ, dengan kejadian penyakit Tuberkulosis Paru. Observasi analitik dengan rancangan potong silang yang membandingkan kadar serum IFN-γ, fungsi dan ekspresi reseptor IFN-γ, pada pasien TB Paru dengan pasangannya yang sehat, serta menganalisis korelasi antara variabel-variabel tersebut dengan kejadian penyakit TB Paru. Penelitian dilakukan pada Juli 2009 sampai September 2010, di RS Immanuel Bandung dan laboratorium Stem Cell & Cancer Institute, Jakarta. Dibandingkan pasangan hidupnya, kadar serum IFN-γ pasien TB tidak berbeda bermakna, ekspresi reseptor IFN-γ pasien TB lebih tinggi (p=0,041), sedangkan fungsi reseptor IFN-γ pasien TB lebih rendah (p=0,011). Analisis korelasi mendapatkan satusatunya variabel yang mempunyai korelasi bermakna dengan kejadian tuberkulosis paru adalah fungsi reseptor IFN-γ yang rendah (p=0,026, OR 5,56). Pada pasien TB Paru ekspresi reseptor IFN-γ lebih tinggi, tetapi fungsi reseptor IFN-γ lebih rendah dari pasangan hidup sehat. Fungsi reseptor IFN-γ yang rendah ini mempunyai korelasi bermakna dengan kejadian tuberkulosis paru.Kata kunci: tuberkulosis, kadar serum IFN-γ, ekspresi dan fungsi reseptor IFN-γ.AbstractIn order to work effectively against M. tuberculosis, the cell mediated immune system needs serum level of IFN-γ and its receptors in the surface of mononuclear cells to function optimally. The objective of this study is to analyze the correlation of IFNγ serum level, expression and function of IFN-γ receptor, with pulmonary tuberculosis. Analytical descriptive method with cross sectional design that compared the serum level of IFN-γ, function and expression of IFN-γ receptor in pulmonary tuberculosis patients with their healthy spouses, and analyzing the correlation between these variables with pulmonary tuberculosis. Study was done from July 2009 until September 2010, in Immanuel hospital Bandung and Stem Cell & Cancer Institute Jakarta. Compared with their healthy spouses the IFN-γ serum level in TB patients was not different statistically, the IFN-γ receptor expression in TB patients was higher (p=0,041), however, the IFN-γ receptor function of TB patients was lower (p=0,011). Correlation analysis showed that the only variable had correlation significantly with pulmonary tuberculosis was low function of IFN-γ receptor (p=0,026,OR 5,56). The expression of IFN-γ receptor in pulmonary tuberculosis patients was higher, while the function of IFN-γ receptor was lower than their healthy spouses. The low function of IFN-γ receptor had significantly correlation with pulmonary tuberculosis. Key Words: Tuberculosis, IFN-γ serum level, IFN-γ receptor expression and function.

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