scholarly journals Control of residues of thyreostats in slaughter animals in Poland in 2011–2017

2018 ◽  
Vol 62 (4) ◽  
pp. 511-517
Author(s):  
Barbara Woźniak ◽  
Iwona Matraszek-Żuchowska ◽  
Katarzyna Sielska ◽  
Sebastian Witek ◽  
Andrzej Posyniak ◽  
...  
Keyword(s):  
Urine Samples ◽  
Animal Origin ◽  
Tandem Mass ◽  
Consumer Health ◽  
The European Union ◽  
Control Plan ◽  
Chromatography Tandem Mass Spectrometry ◽  
Council Directive ◽  
Liquid Chromatography Tandem Mass ◽  
Cruciferous Plants

AbstractIntroduction: In the European Union, the use of thyreostatic drugs for fattening slaughter animals has been banned since 1981 under Council Directive 81/602/EEC. For protection of consumer health against unwanted residues and in compliance with Directive 96/23, each EU country must monitor thyreostats in samples of animal origin. This paper presents the results of research on thyreostatic residues carried out in Poland in 2011–2017.Material and Methods: The material for testing was urine (n = 3,491), drinking water (n = 127), and muscle samples (n = 349) officially collected by Veterinary Sanitary Inspectors in slaughterhouses and farms throughout the country in accordance with the national residue control plan. The samples were examined for the presence of tapazole, thiouracil, methylthiouracil, propylthiouracil, and phenylthiouracil using liquid chromatography tandem mass spectrometry through an accredited method.Results: In four bovine and three porcine urine samples, the permissible thiouracil concentration was exceeded. In one sample of porcine urine, methyl- and propylthiouracil were found. The presence of thiouracil and its derivatives in urine samples is most likely due to feeding animals diet containing cruciferous plants.Conclusions: The results of research indicate that thyreostats are not used for anabolic purposes in slaughter animals in Poland.

scholarly journals Urinary Concentrations of Steroids in Bulls under Anabolic Treatment by Revalor-XS® Implant

2016 ◽  
Vol 2016 ◽  
pp. 1-16 ◽  
Author(s):  
Giancarlo Biancotto ◽  
Roberto Stella ◽  
Federica Barrucci ◽  
Francesca Lega ◽  
Roberto Angeletti
Keyword(s):  
Time Range ◽  
Tandem Mass ◽  
Endogenous Hormones ◽  
The European Union ◽  
Growth Promoters ◽  
Trenbolone Acetate ◽  
Chromatography Tandem Mass Spectrometry ◽  
Anabolic Treatment ◽  
Highly Sensitive ◽  
Liquid Chromatography Tandem Mass

Despite the European ban of using anabolics in food-producing animals, growth promoters might still be illegally used in the European Union. To control the food chain and guarantee consumers’ health, there is a need of highly sensitive analytical methods for the identification of marker residues of such treatments. In the present study, a group of bulls (n=16) received trenbolone acetate (200 mg) and estradiol (40 mg) by a commercial ear implant during a time range of 71 days, and a second group (n=16) was kept for control. The aim of the research was to measure the residual urinary concentrations of the administered drugs (β-trenbolone andβ-estradiol), their main metabolites (α-trenbolone andα-estradiol), and possible alterations of the urinary profile of other endogenous hormones metabolically related. The analytical method was based on liquid chromatography-tandem mass spectrometry. Results showed average urinary concentrations ofα-trenbolone andα-estradiol during treatment in the range of (0.81÷2.1) ng mL−1and (0.96÷4.4) ng mL−1, respectively, whereasβ-trenbolone andβ-estradiol exhibit urinary concentrations lower than 0.22 ng mL−1in both cases. Data obtained from the urinary profiles of endogenous steroids indicate that they could be useful to indirectly detect the ongoing treatment.

scholarly journals Determination of Urinary Creatinine in Washington State Residents via Liquid Chromatography/Tandem Mass Spectrometry

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Caroline E. West ◽  
Blaine N. Rhodes
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Washington State ◽  
Urine Samples ◽  
Tandem Mass ◽  
Urinary Creatinine ◽  
Spot Urine ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass

A viable, quick, and reliable method for determining urinary creatinine by liquid chromatography/tandem mass spectrometry (LC/MS/MS) was developed and used to evaluate spot urine samples collected for the Washington Environmental Biomonitoring Survey (WEBS): part of the Washington State Department of Health, Public Health Laboratories (PHL). 50 µL of urine was mixed with a 1 : 1 acetonitrile/water solution containing deuterated creatinine as the internal standard and then analyzed by LC/MS/MS. Utilizing electrospray ionization (ESI) in positive mode, the transition ions for creatinine and creatinine-d3were determined to be 114.0 to 44.1 (quantifier), 114.0 to 86.1 (qualifier), and 117.0 to 47.1 (creatinine-d3). The retention time for creatinine was 0.85 minutes. The linear calibration range was 20–4000 mg/L, with a limit of detection at 1.77 mg/L and a limit of quantitation at 5.91 mg/L. LC/MS/MS and the colorimetric Jaffé reaction were associated significantly (Pearsonr=0.9898andR2=0.9797,ρ≤0.0001). The LC/MS/MS method developed at the PHL to determine creatinine in the spot urine samples had shorter retention times, and was more sensitive, reliable, reproducible, and safer than other LC/MS/MS or commercial methods such as the Jaffé reaction or modified versions thereof.

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