Antioxidant Status of Sprague-Dawley Female Rat with Curcuminoids Nanoparticles of Balittro Curcuma

2017 ◽  
Vol 4 (1) ◽  
pp. 23-32
Author(s):  
Laksmi Ambarsari ◽  
Riska Febrianti ◽  
Edy Djauhari Purwakusumah
Keyword(s):  
Antioxidant Status ◽  
Female Rat ◽  
Sprague Dawley

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scholarly journals Antioxidant Status of Sprague-Dawley Female Rat with Curcuminoids Nanoparticles of Balittro Curcuma

2020 ◽  
Vol 6 (1) ◽  
Author(s):  
Laksmi Ambarsari ◽  
Riska Febrianti ◽  
Edy Djauhari Purwakusumah
Keyword(s):  
Antioxidant Activity ◽  
Particle Size ◽  
Superoxide Dismutase ◽  
Antioxidant Status ◽  
Clinical Symptoms ◽  
Entrapment Efficiency ◽  
Female Rat ◽  
Solid Lipid Nanoparticle ◽  
Sprague Dawley ◽  
Curcuma Xanthorrhiza

Curcuma (Curcuma xanthorrhiza) is an Indonesian herbs plant with antioxidant activity. This research aimed to measure the increase of curcuminoids bioavailability through the effectivity of nanoparticle curcuminoids as an antioxidant for carbon tetrachloride (CCl4) induced rats. Nanoparticle curcuminoids were produced by homogenization-ultrasonication method showed results particle size of curcuminoids nanoparticle was 141.85±38.82 nm with polydispersity index was 0.233 and entrapment efficiency was 71%. During the treatment, the rat’s body weight was increased. Clinical symptoms of rats (behavior and feces) were normal. Nanoparticle curcuminoids can decrease malondialdehyde concentrations and increase peroxide, glutathione peroxide, and superoxide dismutase activity.   Keywords :  antioxidant, curcuminoid, solid lipid nanoparticle, Curcuma.

scholarly journals EFFICACY OF TRIDHAM AND 1,2,3,4,6-PENTA-O-GALLOYL-β-D-GLUCOSE IN REVERSING LIPID PEROXIDATION LEVELS AND MITOCHONDRIAL ANTIOXIDANT STATUS IN 7,12-DIMETHYLBENZENEANTHRACENE (DMBA) INDUCED BREAST CANCER IN SPRAGUE-DAWLEY RATS

2016 ◽  
Vol 8 (9) ◽  
pp. 288 ◽  
Author(s):  
Stalin Ramakrishnan ◽  
Karthick Dharmalingam ◽  
Sachidanandham T Panchanatham ◽  
Shanthi Palanivelu
Keyword(s):  
Lipid Peroxidation ◽  
Therapeutic Effect ◽  
Mammary Carcinoma ◽  
Antioxidant Status ◽  
Albino Rats ◽  
Histopathological Analysis ◽  
Sprague Dawley ◽  
Standard Drug ◽  
Gastric Intubation ◽  
Group I

<p><strong>Objective: </strong>To determine the effect of <em>Tridham</em> (TD) and 1,2,3,4,6-penta-O-galloyl-β-d-glucose(PGG) on lipid peroxidation levels and mitochondrial antioxidants status in experimental mammary carcinoma.</p><p><strong>Methods</strong>:<strong> </strong><em>Elaecoarpus ganitrus </em>(fruits), <em>Terminalia chebula </em>(seed coats), <em>Prosopis cineraria </em>(leaves)<em>, </em>adult female albino rats of Sprague-Dawley strain weighing 170–190 g and 7,12-dimethylbenzeneanthracene (DMBA) were used for this study. Group I control rats, Group II rats mammary carcinoma induced with DMBA (25 mg in 1 ml olive oil) by gastric intubation. Group III, IV and V DMBA induced rats were treated with TD (400 mg/kg. b. wt/day), PGG (30 mg/kg. b. wt/day) and standard drug, Cyclophosphamide (30 mg/kg. b. wt/day), respectively for 48 d by gastric intubation. Group VI and VII rats served as TD and PGG treated controls, respectively for 48 d by gastric intubation. At the end of the experimental period, the rats were anaesthetized and sacrificed. Mammary glands were isolated and used for biochemical assays and histopathological evaluation.</p><p><strong>Results: </strong>In rats with cancer, the lipid peroxide levels (LPO) were significantly increased and mitochondrial antioxidant levels were decreased. Treatment with TD and PGG decreased LPO levels and increased mitochondrial antioxidant status in mammary carcinoma bearing rats. Histopathological analysis also confirmed the therapeutic effect of TD and PGG. No significant adverse effect was observed in sole drug treated group of rats.</p><p><strong>Conclusion: </strong>TD and PGG have definite therapeutic effect in experimental mammary carcinoma and inhibit growth of cancer cells by restoring mitochondrial antioxidant status and energy metabolism to normal states.</p>

Infarct size is increased in female post-MI rats treated with rapamycin

2009 ◽  
Vol 87 (6) ◽  
pp. 460-470 ◽  
Author(s):  
Claude Lajoie ◽  
Viviane El-Helou ◽  
Cindy Proulx ◽  
Robert Clément ◽  
Hugues Gosselin ◽  
...  
Keyword(s):  
Left Ventricle ◽  
Female Rats ◽  
Coronary Artery Ligation ◽  
Female Rat ◽  
Sprague Dawley ◽  
Ischemic Insult ◽  
Fibrotic Response ◽  
Artery Ligation ◽  
Sprague Dawley Rats ◽  
Scar Healing

Rapamycin represents a recognized drug-based therapeutic approach to treat cardiovascular disease. However, at least in the female heart, rapamycin may suppress the recruitment of putative signalling events conferring cardioprotection. The present study tested the hypothesis that rapamycin-sensitive signalling events contributed to the cardioprotective phenotype of the female rat heart after an ischemic insult. Rapamycin (1.5 mg/kg) was administered to adult female Sprague–Dawley rats 24 h after complete coronary artery ligation and continued for 6 days. Rapamycin abrogated p70S6K phosphorylation in the left ventricle of sham rats and the noninfarcted left ventricle (NILV) of 1-week postmyocardial-infarcted (MI) rats. Scar weight (MI 0.028 ± 0.006, MI+rapamycin 0.064 ± 0.004 g) and surface area (MI 0.37 ± 0.08, MI+rapamycin 0.74 ± 0.03 cm2) were significantly larger in rapamycin-treated post-MI rats. In the NILV of post-MI female rats, rapamycin inhibited the upregulation of eNOS. Furthermore, the increased expression of collagen and TGF-β3 mRNAs in the NILV were attenuated in rapamycin-treated post-MI rats, whereas scar healing was unaffected. The present study has demonstrated that rapamycin-sensitive signalling events were implicated in scar formation and reactive fibrosis. Rapamycin-mediated suppression of eNOS and TGF-β3 mRNA in post-MI female rats may have directly contributed to the larger infarct and attenuation of the reactive fibrotic response, respectively.

Abstract 579: Increased Role of COX-derived Prostanoids in Regulating Ang II Induced Uterine Artery Contraction at Early Pregnancy in a Transgenic Model of Preeclampsia

Hypertension ◽  
2014 ◽  
Vol 64 (suppl_1) ◽  
Author(s):  
Victor M Pulgar ◽  
Liliya M Yamaleyeva ◽  
Jasmina Varagic ◽  
Carolynne M McGee ◽  
Michael Bader ◽  
...  
Keyword(s):  
Uterine Artery ◽  
Inhibitory Effect ◽  
Isometric Tension ◽  
Late Gestation ◽  
Female Rat ◽  
Ang Ii ◽  
Sprague Dawley ◽  
Vascular Control ◽  
Human Renin

The balance between vasodilatory and vasoconstrictor prostanoids contributes to vascular control during pregnancy. Alterations in this balance are involved in the development of hypertensive pregnancy. The transgenic female rat containing the human angiotensinogen (hAGN) gene mated with the male transgenic containing human renin (hREN) is a model of preeclampsia (TgA), and shows hypertension and proteinuria at late gestation. We investigated the role COX-derived mediators have on contractility of the uterine artery (UA) in TgA rats before the hypertensive phenotype develops. UA were isolated from transgenic TgA (n=9) and Sprague-Dawley (n=7) control rats at 7 days of gestation. UA were mounted in a wire myograph for determinations of isometric tension (DMT USA, 620M). Responses to acetylcholine (ACh), phenylephrine (Phe) and sodium nitroprusside (SNP) were measured in control conditions and after preincubation with indomethacin (Indo, 10-5M). Data were fitted to a dose response curve, vasodilatation was expressed as percent of pre-constriction and sensitivity as pD2 (pD2= -Log [EC50]). Responses to ACh reached similar maximal relaxations (64±8 vs 75±6%, p>0.05), and an increased contraction in TgA UA at ACh >10μM (p<0.05) was eliminated by Indo. Contraction to Phe was similar in both groups with an inhibitory effect of Indo on TgA UA (p<0.05). Relaxation to SNP was lower in TgA vs SD UA (92±2 vs 74±5%, p<0.05), this difference was abolished by Indo. Thus, inhibition of COX enzymes had a greater effect on TgA UA suggesting an imbalance towards an increased prostanoids-derived constrictor tone in TgA UA. This imbalance appears before the hypertensive phenotype is established.

Influence of nicotine on the core temperature response to a novel environment in pregnant rats

1997 ◽  
Vol 83 (5) ◽  
pp. 1612-1616 ◽  
Author(s):  
James E. Fewell ◽  
Patricia A. Tang
Keyword(s):  
Open Field ◽  
Core Temperature ◽  
Temperature Response ◽  
Chronic Administration ◽  
Female Rat ◽  
Sprague Dawley ◽  
Pregnant Rats ◽  
Temperature Index ◽  
Novel Environment ◽  
The Core

Fewell, James E., and Patricia A. Tang. Influence of nicotine on the core temperature response to a novel environment in pregnant rats. J. Appl. Physiol.83(5): 1612–1616, 1997.—Exposure of a male or nonpregnant female rat to a novel environment, such as a simulated open field, induces a transient increase in core temperature, which is often called stress-induced hyperthermia. Pregnancy alters this response such that the core temperature index increases significantly during exposure to a simulated open field on day 10 but not on days 15 and 20 of gestation in rats. The present experiments were carried to investigate the effect of chronic administration of nicotine (0, 1, 2, 4, or 8 mg ⋅ kg−1 ⋅ 24 h−1 for 13–15 days) on the core temperature response to a simulated open field in chronically instrumented pregnant ( day 20 or 21 of gestation) and nonpregnant Sprague-Dawley rats. In nonpregnant rats, the core temperature index increased more during exposure to a simulated open field after chronic administration of nicotine at all doses than after chronic administration of vehicle; the core temperature response was not dependent on the dose of nicotine. In pregnant rats, significant increases in core temperature as well as in the core temperature index occurred only during exposure to a simulated open field after chronic administration of nicotine in doses of 2, 4, or 8 mg ⋅ kg−1 ⋅ 24 h−1; the core temperature response was dependent on the dose of nicotine. Our data provide evidence that chronic exposure to nicotine enhances the core temperature response to a simulated open field in nonpregnant rats and unmasks a maternal thermogenic response that is not seen to the same stimulus near term of pregnancy. The possible physiological consequences for the fetus are presently unknown and require investigation.

scholarly journals Fluoxetine Dose and Administration Method Differentially Affect Hippocampal Plasticity in Adult Female Rats

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Jodi L. Pawluski ◽  
Eva van Donkelaar ◽  
Zipporah Abrams ◽  
Virginie Houbart ◽  
Marianne Fillet ◽  
...  
Keyword(s):  
Adult Female ◽  
Female Rats ◽  
Granule Cell Layer ◽  
Female Rat ◽  
Sprague Dawley ◽  
Osmotic Minipump ◽  
Hippocampal Plasticity ◽  
Fluoxetine Treatment ◽  
Administration Method ◽  
Administration Methods

Selective serotonin reuptake inhibitor medications are one of the most common treatments for mood disorders. In humans, these medications are taken orally, usually once per day. Unfortunately, administration of antidepressant medications in rodent models is often through injection, oral gavage, or minipump implant, all relatively stressful procedures. The aim of the present study was to investigate how administration of the commonly used SSRI, fluoxetine, via a wafer cookie, compares to fluoxetine administration using an osmotic minipump, with regards to serum drug levels and hippocampal plasticity. For this experiment, adult female Sprague-Dawley rats were divided over the two administration methods: (1) cookie and (2) osmotic minipump and three fluoxetine treatment doses: 0, 5, or 10 mg/kg/day. Results show that a fluoxetine dose of 5 mg/kg/day, but not 10 mg/kg/day, results in comparable serum levels of fluoxetine and its active metabolite norfluoxetine between the two administration methods. Furthermore, minipump administration of fluoxetine resulted in higher levels of cell proliferation in the granule cell layer (GCL) at a 5 mg dose compared to a 10 mg dose. Synaptophysin expression in the GCL, but not CA3, was significantly lower after fluoxetine treatment, regardless of administration method. These data suggest that the administration method and dose of fluoxetine can differentially affect hippocampal plasticity in the adult female rat.

scholarly journals CLARITY-BPA: Bisphenol A or Propylthiouracil on Thyroid Function and Effects in the Developing Male and Female Rat Brain

Endocrinology ◽  
2019 ◽  
Vol 160 (8) ◽  
pp. 1771-1785 ◽  
Author(s):  
Ruby Bansal ◽  
R Thomas Zoeller
Keyword(s):  
Thyroid Hormone ◽  
Bisphenol A ◽  
Ethinyl Estradiol ◽  
Elevated Serum ◽  
Developing Brain ◽  
Female Rat ◽  
Sprague Dawley ◽  
Negative Effects ◽  
End Points ◽  
Environmental Health Sciences

Abstract The CLARITY-BPA experiment, a large collaboration between the National Institute of Environmental Health Sciences, the National Toxicology Program, and the US Food and Drug Administration, is designed to test the effects of bisphenol A (BPA) on a variety of endocrine systems and end points. The specific aim of this subproject was to test the effect of BPA exposure on thyroid functions and thyroid hormone action in the developing brain. Timed-pregnant National Center for Toxicological Research Sprague-Dawley rats (strain code 23) were dosed by gavage with vehicle control (0.3% carboxymethylcellulose) or one of five doses of BPA [2.5, 25, 250, 2500, or 25,000 µg/kg body weight (bw) per day] or ethinyl estradiol (EE) at 0.05 or 0.50 µg/kg bw/d (n = 8 for each group) beginning on gestational day 6. Beginning on postnatal day (PND) 1 (day of birth is PND 0), the pups were directly gavaged with the same dose of vehicle, BPA, or EE. We also obtained a group of animals treated with 3 ppm propylthiouracil in the drinking water and an equal number of concordant controls. Neither BPA nor EE affected serum thyroid hormones or thyroid hormone‒sensitive end points in the developing brain at PND 15. In contrast, propylthiouracil (PTU) reduced serum T4 to the expected degree (80% reduction) and elevated serum TSH. Few effects of PTU were observed in the male brain and none in the female brain. As a result, it is difficult to interpret the negative effects of BPA on the thyroid in this rat strain because the thyroid system appears to respond differently from that of other rat strains.

scholarly journals Greater natriuretic response to ENaC inhibition in male versus female Sprague-Dawley rats

2020 ◽  
Vol 318 (2) ◽  
pp. R418-R427 ◽  
Author(s):  
Reham H. Soliman ◽  
Jermaine G. Johnston ◽  
Eman Y. Gohar ◽  
Crystal M. Taylor ◽  
David M. Pollock
Keyword(s):  
Protein Expression ◽  
Time Of Day ◽  
Female Rats ◽  
Female Rat ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Endothelin System ◽  
Males And Females ◽  
Epithelial Sodium Channel Enac

Genes for the epithelial sodium channel (ENaC) subunits are expressed in a circadian manner, but whether this results in time-of-day differences in activity is not known. Recent data show that protein expression of ENaC subunits is higher in kidneys from female rats, yet females are more efficient in excreting an acute salt load. Thus, our in vivo study determined whether there is a time-of-day difference as well as a sex difference in the response to ENaC inhibition by benzamil. Our results showed that the natriuretic and diuretic responses to a single dose of benzamil were significantly greater in male compared with female rats whether given at the beginning of the inactive period [Zeitgeber time 0 (ZT0), 7 AM] or active period (ZT12, 7 PM). However, the response to benzamil was not significantly different between ZT0 and ZT12 dosing in either male or female rats. There was no difference in renal cortical α-ENaC protein abundance between ZT0 and ZT12 or males and females. Given previous reports of flow-induced stimulation of endothelin-1 (ET-1) production and sex differences in the renal endothelin system, we measured urinary ET-1 excretion to assess the effects of increased urine flow on intrarenal ET-1. ET-1 excretion was significantly increased following benzamil administration in both sexes, but this increase was significantly greater in females. These results support the hypothesis that ENaC activity is less prominent in maintaining Na+ balance in females independent of renal ET-1. Because ENaC subunit genes and protein expression vary by time of day and are greater in female rat kidneys, this suggests a clear disconnect between ENaC expression and channel activity.

scholarly journals Bioavailability of Melatonin from Lentil Sprouts and Its Role in the Plasmatic Antioxidant Status in Rats

Foods ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 330 ◽  
Author(s):  
Miguel Rebollo-Hernanz ◽  
Yolanda Aguilera ◽  
Teresa Herrera ◽  
L. Tábata Cayuelas ◽  
Montserrat Dueñas ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Antioxidant Status ◽  
Pharmacokinetic Profile ◽  
Total Phenolic ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Melatonin Administration ◽  
Kaempferol Glycosides ◽  
First Time ◽  
Related Compounds

Melatonin is a multifunctional antioxidant neurohormone found in plant foods such as lentil sprouts. We aim to evaluate the effect of lentil sprout intake on the plasmatic levels of melatonin and metabolically related compounds (plasmatic serotonin and urinary 6-sulfatoxymelatonin), total phenolic compounds, and plasmatic antioxidant status, and compare it with synthetic melatonin. The germination of lentils increases the content of melatonin. However, the phenolic content diminished due to the loss of phenolic acids and flavan-3-ols. The flavonol content remained unaltered, being the main phenolic family in lentil sprouts, primarily composed of kaempferol glycosides. Sprague Dawley rats were used to investigate the pharmacokinetic profile of melatonin after oral administration of a lentil sprout extract and to evaluate plasma and urine melatonin and related biomarkers and antioxidant capacity. Melatonin showed maximum concentration (45.4 pg/mL) 90 min after lentil sprout administration. The plasmatic melatonin levels increased after lentil sprout intake (70%, p < 0.05) with respect to the control, 1.2-fold more than after synthetic melatonin ingestion. These increments correlated with urinary 6-sulfatoxymelatonin content (p < 0.05), a key biomarker of plasmatic melatonin. Nonetheless, the phenolic compound content did not exhibit any significant variation. Plasmatic antioxidant status increased in the antioxidant capacity upon both lentil sprout and synthetic melatonin administration. For the first time, we investigated the bioavailability of melatonin from lentil sprouts and its role in plasmatic antioxidant status. We concluded that their intake could increase melatonin plasmatic concentration and attenuate plasmatic oxidative stress.

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