Vitamin B12 deficiency is linked with dyslipidemia in gestational diabetes mellitus.

Objective: To determine the vitamin B12 deficiency and dyslipidemia in Gestational Diabetes mellitus (GDM) diagnosed pregnant women. Study Design: Observational study. Setting: Department of Biochemistry and Gynecology/Obstetrics LUMHS Hospital Jamshoro. Period: January 2018 to December 2018. Material & Methods: A sample of 216 diagnosed GDM pregnant women was selected according to study criteria. Venous blood samples were centrifuged to separate sera; that were used for the estimation of (hexokinase method), blood lipids and Vitamin B12 (ECLIA assay method) by Cobas chemistry analyzer. Statistical SPSS software 21.0 (IBM, Inc USA) was used for study research variables at 95% CI (P ≤ 0.05). Results: Age of GDM cases was 36.12±9.5 years. Mean+/-SD vitamin B12 level was noted 154.7±81.7 ng/mL (P=0.0001). Serum cholesterol, triglycerides and LDLc were elevated and HDLc was low in GDM cases (P=0.0001). Of 216 GDM cases, vitamin B12 deficiency was present in 152 (70.3%) (P=0.0001) and dyslipidemia in 50 (23.1%) (X2=452.0) (P=0.0001). Vitamin B12 shows inverse correlation with RBG (r= -0.41, P=0.005), CHOL (r= -0.25, P=0.024), TAG (r= -0.81, P=0.0001), LDLc (r= -0.797, P=0.0001) and positive correlation with HDLc (r= 0.76, P=0.0001). Conclusion: The present study finds vitamin B12 deficiency in 152 (70.3%) and dyslipidemia in 50 (23.1%). Vitamin B12 deficient GDM women show high cholesterol, triglycerides, LDLc and low HDLc. Hence, it is concluded, the vitamin B12 deficiency is linked with dyslipidemia in Gestational Diabetes mellitus.

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Frequency of Vitamin B12 Deficiency in Pregnant Women with Gestational Diabetes Mellitus

10.53350/pjmhs2115102753 ◽

2021 ◽

Vol 15 (10) ◽

pp. 2753-2755

Author(s):

Shazia Jamil ◽

Naveed Mahmood ◽

Israr-ul- Haque ◽

Rabiah Haque ◽

Muhammad Imran-ul-Hasan ◽

...

Keyword(s):

Diabetes Mellitus ◽

Gestational Diabetes ◽

Gestational Diabetes Mellitus ◽

Vitamin B12 ◽

Pregnant Women ◽

Vitamin B12 Deficiency ◽

Group I ◽

B12 Deficiency ◽

Vitamin B 12 ◽

Group Ii

Objective: To determine the prevalence of vitamin B-12 deficiency in pregnant women with gestational diabetes mellitus. Study Design: Retrospective study Place and Duration of Study: Department of Medicine, OMC Hospital, Jail Road Lahore from January, 2020 to December, 2020. Methods: Two hundred and thirty pregnant females were enrolled age between 18-45 years. Detailed demographics of enrolled cases age, gestational age, gravidity and body mass index were recorded after taking informed written consent. Among 230 cases, 100 women were non GDM (group I) and 130 had gestational diabetes mellitus (group II). Blood samples were taken from all the patients for measuring vitamin B12 status. Vitamin B12 was defined as <300 pg/ml. Results: Mean age of the patients in group I was 31.64±7.45 years with mean BMI 25.88±8.65 kg/m2 while mean age among GDM group was 34.55±5.71 years with mean BMI 27.36±9.44 kg/m2. Mean gestational age in group I was 33.72±4.21 weeks and in group II 35.08±9.27 weeks. In group I 20 (20%) had vitamin B12 deficiency and in group II rate ofvitamin B12 deficiency was high among 90 (69.2%) cases. We found a significantly relation between vitamin B12 and GDM with p value <0.0007. Conclusion: The prevalence of vitamin B-12 deficiency among pregnant women of gestational diabetes mellitus was significantly high as compared to normal pregnant women. Keywords: Pregnant Women, Gestational Diabetes Mellitus, Vitamin B12 deficiency

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Frequency of Hypovitaminosis B12 in Pregnant Women with Gestational Diabetes Mellitus

10.53350/pjmhs211572296 ◽

2021 ◽

Vol 15 (7) ◽

pp. 2296-2298

Author(s):

Aesha Sadaf Rizwan ◽

Kousar Robeen ◽

Afshan Ahmad ◽

Irum Batool Hashmi ◽

Muhammad Tahir ◽

...

Keyword(s):

Diabetes Mellitus ◽

Gestational Diabetes ◽

Gestational Diabetes Mellitus ◽

Vitamin B12 ◽

Pregnant Women ◽

Gestational Age ◽

Vitamin B12 Deficiency ◽

High Rate ◽

P Value ◽

B12 Deficiency

Objective: To determine the frequency of hypovitaminosis B12 in pregnant females presented with gestational diabetes mellitus. Study Design: Retrospective study Place and Duration: The department of Gynaecology and Obstetrics, Category A Hospital, Batkhela Malakand and Muhammad Teaching Hospital, Peshawarfor the duration of six months from October 2020 to March 2021. Methodology: Total 190 pregnant women (95 GDM, 95 non-GDM) were enrolled in this study. Patient’s ages were ranging between 18 to 40 years. Detailed demographics including age, gestational age, body mass index (BMI), and gravidty were recorded after taking informed written consent. Blood samples were taken from all the patients for measuring vitamin B12 status. Hypovitaminosis B12 was defined as <300 pg/ml. Data was analyzed by SPSS 24.0. Results: Mean age of patients was 32.46±6.82 years. Mean BMI was 26.24±2.34 kg/m2. Mean gestational age was 34.56±2.18 weeks. 105 (55.26%) women were multigravida. 80 (42.11%) patients had vitamin B12 level <300 pg/ml. Patients with GDM had high rate of vitaminosis B12 deficiency as compared to non-GDM patients (65.26% Vs 18.95%). A significant association was observed between GDM and hypovitaminosis B12 with p-value <0.0001. Conclusion: Vitamin B12 deficiency was significantly associated with gestational diabetes mellitus with frequency of 65.26%. Keywords: Vitaminosis B12, Gestational Diabetes Mellitus, Pregnancy

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Serum cobalamin status of pregnant women suffering from gestational diabetes mellitus.

The Professional Medical Journal ◽

10.29309/tpmj/2020.27.05.4076 ◽

2020 ◽

Vol 27 (05) ◽

pp. 1004-1010

Author(s):

Saher Fatima ◽

Sadia Saeed ◽

Syeda Fariha Hasnny ◽

Nathumal Maheshwari ◽

Urooj Tabassum ◽

...

Keyword(s):

Diabetes Mellitus ◽

Blood Glucose ◽

Glycemic Control ◽

Gestational Diabetes ◽

Gestational Diabetes Mellitus ◽

Pregnant Women ◽

Fasting Blood Glucose ◽

Inverse Correlation ◽

Cobalamin Deficiency ◽

Assay Method

Objectives: Determining serum cobalamin levels in Pregnant Women suffering from Gestational Diabetes mellitus (GDM) presenting at our tertiary care hospital. Study Design: Case control study. Setting: Department of Gynecology and Medicine, SMBB Medical College Layari General Hospital Karachi. Period: January 2016 to April 2017. Material & Methods: Sample of 100 pregnant women in 2nd and 3rd trimester was selected into; 50 controls and 50 GDM cases through convenient sampling. GDM was defined as pregnant women with fasting blood sugar ≥100 mg/dL. 5 ml blood was collected; 3 ml put into EDTA tubes for complete blood counts and 2 ml for sera. Blood glucose was estimated by hexokinase method, HbA1c by colorimetric method and cobalamin by ECLIA assay method. SPSS software 21.0 (IBM, Inc USA) was used for data analysis using Student t-test and Chi-square test (P ≤ 0.05). Results: Age of control was 30.43±1.49 years and 29.95±1.27 years in cases. Gestational age was 33.67±2.69 weeks in controls and 34.75±2.53 weeks in cases. Control and cases shows serum cobalamin levels of 316.34± 113.77 pg/ml and 253.5±121.32 pg/ml respectively (P=0.009). Serum cobalamin deficiency was noted in 68% of cases and 40% of controls (P<0.05). Glycemic control was bad in majority of cases. Serum cobalamin shows inverse correlation with random blood glucose, fasting blood glucose and Glycated HbA1. Conclusion: We found low serum cobalamin levels in pregnant women suffering from gestational diabetes mellitus that showed inverse correlation with random and fasting blood glucose and glycemic control.

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Cognitive Dysfunction of Pregnant Women with Gestational Diabetes Mellitus in Perinatal Period

Journal of Healthcare Engineering ◽

10.1155/2021/2302379 ◽

2021 ◽

Vol 2021 ◽

pp. 1-6

Author(s):

Siriguleng Sana ◽

Xijin Deng ◽

Lei Guo ◽

Xunhong Wang ◽

Enyou Li

Keyword(s):

Diabetes Mellitus ◽

Blood Glucose ◽

Cognitive Function ◽

Gestational Diabetes ◽

Gestational Diabetes Mellitus ◽

Pregnant Women ◽

Cognitive Dysfunction ◽

Statistical Significance ◽

Venous Blood ◽

Perinatal Period

Purpose. To explore whether pregnant women with gestational diabetes mellitus (GDM) had cognitive impairment and assess cognitive function in normal pregnant women. Methods. A total of 75 consecutive women diagnosed with GDM (GDM group), 70 normal pregnant women (NP group) without diabetes and matched for age, and 51 female volunteers (CG group) with the similar age level, normal blood glucose, and nonpregnancy were included in the study. For the assessment of cognitive functions, Montreal Cognitive Assessment (MoCA) was performed. Venous blood samples were collected to measure blood glucose, glycated hemoglobin (HbA1c), methylglyoxal (MGO), beta amyloid (Aβ), and tau protein. Results. The score of MoCA of GDM was lowest, and the score of the NP group was lower than volunteers ( P < 0.05 ). The incidence of cognitive dysfunction increased significantly in the GDM group with statistical significance ( P < 0.05 ). The levels of tau and MGO in the GDM group were significantly less than those in the NP and CG groups, and Aβ in the GDM group was significantly more than that in the NP and CG groups ( P < 0.05 ), but the differences between NP and CG groups were not statistically significant ( P < 0.05 ). Conclusion. The pregnant women with GDM showed a significant decline in cognitive function, and the normal pregnant women also showed a decline in cognitive function which is very light.

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Expression of H2S in Gestational Diabetes Mellitus and Correlation Analysis with Inflammatory Markers IL-6 and TNF-α

Journal of Diabetes Research ◽

10.1155/2020/3085840 ◽

2020 ◽

Vol 2020 ◽

pp. 1-11 ◽

Cited By ~ 2

Author(s):

Yucui Teng ◽

Shuxia Xuan ◽

Ming Jiang ◽

Li Tian ◽

Jinjing Tian ◽

...

Keyword(s):

Diabetes Mellitus ◽

Gestational Diabetes ◽

Gestational Diabetes Mellitus ◽

Pregnant Women ◽

Inflammatory Markers ◽

Umbilical Vein ◽

Venous Blood ◽

Tnf Α ◽

Mother And Child ◽

The Difference

Background. Gestational diabetes mellitus (GDM) is a severe threat to the health of both mother and child. The pathogenesis of GDM remains unclear, although much research has found that the levels of hydrogen sulfide (H2S) play an important role in complications of pregnancy. Methods. We collected venous blood samples from parturient women and umbilical vein blood (UVB) and peripheral venous blood (PVB) samples one hour after childbirth in the control, GDM-, and GDM+ groups in order to determine the concentration of glucose and H2S in plasma; to measure levels of TNF-α, IL-1β, IL-6, TGF-β1, and ADP in parturient women and the UVB of newborns; and to find the correlation of H2S with regression. Results. We found that, with the elevation of glucose, the level of H2S was decreased in GDM pregnant women and newborns and the concentrations of IL-6 and TNF-α were upregulated. With regression, IL-6 and TNF-α concentrations were positively correlated with the level of blood glucose and negatively correlated with H2S concentration. Conclusion. This study shows that downregulation of H2S participates in the pathogenesis of GDM and is of great significance in understanding the difference of H2S between normal and GDM pregnant women and newborns. This study suggests that IL-6 and TNF-α are correlated with gestational diabetes mellitus. The current study expands the knowledge base regarding H2S and provides new avenues for exploring further the pathogenesis of GDM.

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A study of variations in the iron profile and vitamin – b12 levels as predictive bio-chemical markers for gestational diabetes mellitus (gdm) in pregnant women

International Journal of Pharma and Bio Sciences ◽

10.22376/ijpbs.2018.9.4.b78-83 ◽

2018 ◽

Vol 9 (4) ◽

Author(s):

DR. SHIVA GAUTHAM TEJA SUNKARAPALLI ◽

SUCHITRA KAVURI

Keyword(s):

Diabetes Mellitus ◽

Gestational Diabetes ◽

Gestational Diabetes Mellitus ◽

Vitamin B12 ◽

Pregnant Women ◽

Chemical Markers ◽

Iron Profile

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I/D Polymorphism Gene ACE and Risk of Preeclampsia in Women with Gestational Diabetes Mellitus

Disease Markers ◽

10.1155/2020/8875230 ◽

2020 ◽

Vol 2020 ◽

pp. 1-7

Author(s):

O. P. Dmitrenko ◽

N. S. Karpova ◽

M. K. Nurbekov ◽

O. V. Papysheva

Keyword(s):

Diabetes Mellitus ◽

Gestational Diabetes ◽

Gestational Diabetes Mellitus ◽

Pregnant Women ◽

Venous Blood ◽

Adverse Outcomes ◽

Blood Samples ◽

Ace Gene ◽

Large Patient ◽

D Region

Preeclampsia (PE) and gestational diabetes mellitus (GDM) are the most common complications of pregnancy, which result in adverse outcomes for the mother and the fetus. GDM is regarded as a separate independent risk factor for PE development, as evidenced by a higher preeclampsia rate in gestational diabetes mellitus than in the general population. The role the endothelial cell dysfunction plays is considered to be the most reasonable one in the origin of these diseases. The activity of plasma and tissue angiotensin converting enzyme (ACE) is believed to be genetically controlled. The available data suggests that increased ACE activity due to deletion (D)/insertion (I) in the 16th intron of ACE gene, which is called ACE gene I/D polymorphism, is associated with preeclampsia and varies depending on the studied population and the geography. We did not find any literature data that estimates the influence of ACE gene I/D polymorphism on PE rate in pregnant women with GDM. Therefore, the present study aimed to investigate a relationship between ACE gene I/D polymorphism and preeclampsia development in the case of GDM in the Russian population. The study used the genomic DNA derived by phenol-chloroform extraction method from venous blood samples in 137 pregnant women, including samples of 74 women with GDM accompanied with PE and the blood samples of 63 women with GDM w/o preeclampsia. Genotyping of insertion/deletion in the I/D region (16 intron of АСЕ gene) was conducted by real-time PCR using the TaqMan competing probe technology. The particular features in the frequency array of alleles and genotypes of the ACE gen I/D polymorphism under review, as associated with preeclampsia development risk in pregnant women with GDM, were identified. The acquired data testify to the need to further study of ACE gene I/D region polymorphism association in a large patient sample taking into account the PE and GDM risk factors estimated in the clinical practice.

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The Relationship Between Folate, Vitamin B12 and Gestational Diabetes Mellitus with Proposed Mechanisms and Foetal Implications

Journal of Family & Reproductive Health ◽

10.18502/jfrh.v15i3.7131 ◽

2021 ◽

Author(s):

Angeline Maher ◽

Agata Sobczyńska-Malefora

Keyword(s):

Diabetes Mellitus ◽

Insulin Resistance ◽

Gestational Diabetes ◽

Gestational Diabetes Mellitus ◽

Vitamin B12 ◽

Vitamin B12 Deficiency ◽

Folic Acid Supplementation ◽

Homocysteine Concentration ◽

Vitamin B9 ◽

The Impact

The incidence of gestational diabetes mellitus (GDM) is rising, which warrants attention due to the associated complications during pregnancy and in the long term for both mother and offspring. Studies have suggested a relationship between maternal folate (vitamin B9) and vitamin B12 status and GDM risk. Seemingly the most problematic scenario occurs when there is B-vitamin imbalance, with high folate and low vitamin B12. This nutritional state can occur in vitamin B12 deficient women who exceed the recommended folic acid supplementation. However, the pathological mechanisms behind this relationship are currently unclear and are explored in this review article. A high folate/low B12 can lead to a functional folate deficiency through the methyl-trap phenomenon, impairing re-methylation of homocysteine and regeneration of folates for DNA synthesis and repair. Consequently elevated homocysteine concentration leads to endothelial dysfunction and oxidative stress. Vitamin B12 deficiency also leads to an impairment of the conversion of methylmalonyl-CoA to succinyl-CoA, which has been associated with insulin resistance. Insulin resistance is thought to contribute to the etiology of GDM. More studies are needed to confirm the impact of these and other mechanisms on disease development. However, it highlights a potential avenue for GDM risk modification through a vitamin B12 supplement and improvement of maternal metabolic health.

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