scholarly journals Effect of seed priming with kinetin on germination indices and proline activity of Silybum marianum L. under drought stress conditions

2015 ◽  
Vol 9 (1) ◽  
pp. 6-11
Author(s):  
Abolfazl Masoumi Zavariyan ◽  
Mojtaba Yousefi Rad ◽  
Mohsen Asghari

For investigation of the effect of seed priming by kinetin on germination indices and proline activity of Silybum marianum  L. under drought stress, factorial experiment based on completely randomized design with two factor includingdifferent concentrations of kinetin (0, 10 and 20 ppm) and different levels of drought stress including 0, -6 and -12 barat three replicates was carried out. According to the results, drought stress and kinetin individualy cause to decreases of germination percentage, germination rate, seedling length, seed vigor and seedling dry weight but uses of kinetin in drought stress condition improvment of them. Also, drought stress cause to increases of proline content and catalase activity. Therefore, seed priming with 10 ppm kinetin at drought stress was improved the germination indices of Silybum marianum L.DOI: http://dx.doi.org/10.3126/ijls.v9i1.11919 International Journal of Life Sciences Vol.9(1) 2015 6-11

2015 ◽  
Vol 9 (1) ◽  
pp. 17-22 ◽  
Author(s):  
Abolfazl Masoumi Zavariyan ◽  
Mojtaba Yousefi Rad ◽  
Mohsen Asghari

In order to investigation of the effect of seed priming by pyridoxine on germination and biochemical indices of Silybum marianum L. under drought stress, factorial experiment based on completely randomized design with two factor includingdifferent concentrations of pyridoxine including 0, 0.02% and 0.04% and different levels of drought stress including 0, -6 and -12 bar at three replicates was performed. The results indicated that drought stress affected measured indices at P<0.01 and pyridoxine significantly affected germination percentage and rate, seed vigor, mean time germination, seedling length and dry weight at P<0.01 and peroxidase activity at P<0.05. Interaction between the drought stress and pyridoxine was significantly (P<0.05) affected of germination rate and seed vigor. The results showed that with increasing drought stress, germination indices were decreased and biochemical indices were increased. Seed priming with pyridoxine cause to improvement of germination indices and decreases biochemical indices. According to the results, seed priming with 0.04% pyridoxine cause to improvement of germination indices at drought stress conditions.DOI: http://dx.doi.org/10.3126/ijls.v9i1.11921 International Journal of Life Sciences Vol.9(1) 2015 17-22


2021 ◽  
Vol 911 (1) ◽  
pp. 012086
Author(s):  
Ramlah Arief ◽  
Fauziah Koes ◽  
Oom Komalasari ◽  
Fatmawati

Abstract Seed priming or invigoration is presowing treatments of improving germination aimed to reduce the time from sowing to emergence and improving emergence uniformity. Research on seed priming was conducted Indonesian Cereal Research Institute (ICERI) seed laboratory from January until March 2021 to evaluate the effect of seed priming on sorghum seed vigor. Seed quality parameters includes germination percentages, germination rate, shoot and primary root length, seedling dryweight, Sorghum seed variety of Suri 4 were used as seed material. Results showed that among the priming treatments that give positive effect on germination percentage, germination rate, seedling dry weight were priming treatment with KNO3 1.5%.


2017 ◽  
Vol 39 (4) ◽  
pp. 353-362 ◽  
Author(s):  
Mehdi Aghighi Shahverdi ◽  
Heshmat Omidi ◽  
Seyed Jalal Tabatabaei

Abstract: Poor germination and low seedling growth of stevia (Stevia rebuadiana Bertoni) are common problems in the cultivation of the plant. On the other hand, salinity is one of the abiotic environmental stresses that reduce the performance of the product. The objective in this study was to assess the effect of nutri-priming with selenium (Se), iron (Fe) and boron (B) on stevia seed germination indices and physiological characteristics in sodium chloride (NaCl) salinity condition (0, 30, 60 and 90 mM). Results indicated that the effect of salinity in nutri-priming were significant on germination percentage, germination rate, germination uniformity, germination value, seedling length, seedling vigor index, total chlorophyll, free proline content and enzymatic activity of catalase (CAT) and superoxide dismutase (SOD). Salinity stress decreased the percentage and rate of germination and seed vigor, however, nutri-priming reduces the adverse effects of salinity stress. Salinity also reduced the amount of chlorophyll content, in spite of all that this decrease was very low in terms of nutri-priming with Se, Se+Fe and Se+Fe+B in high salinity level (90 mM). Salinity stress increased proline content and antioxidant enzyme activities of CAT and SOD, that nutri-priming especially with the Se more increased. The finding of this study leads to the conclusion that nutri-priming with nutrients, especially Se and Fe increased the antioxidant capacity of the plant to improve germination and seedling growth of stevia under salinity stress.


2017 ◽  
Vol 9 (2) ◽  
pp. 242-249 ◽  
Author(s):  
Faride BEHBOUDI ◽  
Zeinalabedin TAHMASEBI SARVESTANI ◽  
Mohamad Zaman KASSAEE ◽  
Seyed Ali Mohamad MODARES SANAVI ◽  
Ali SOROOSHZADEH

Plants such as wheat and barley that are strategically important crops need to be considered to develop a comprehensive toxicity profile for nanoparticles (NPs). The present study was aimed to investigate the effects of chitosan and SiO2 NPs on wheat and barley plants. Two factorial experiments (seeds priming and direct exposure) were performed based on a completely randomized design in four replications. Results showed that the seeds priming with the NPs had not significant effect on germination parameters such as Germination Percentage (GP), Germination Rate (GR), Germination Value (GV), Mean Germination Time (MGT), Pick Value (PV) and Mean Daily Germination (MDG). In contrast, exposure of the seeds to the NPs had significant effects on these parameters. In both experiments, treatments had significant effects on shoot, seedling, root length, fresh and dry weight, as well as vigor indexes as compared to the control. In most traits, the best concentration of NPs was 30 ppm, whereas applications of the NPs with 90 ppm displayed adverse effects on majority of the studied traits. According to these results, selectivity in applications of NPs with suitable concentration and method is essential for different plant species.  


2019 ◽  
Vol 41 ◽  
pp. e46030
Author(s):  
João Everthon da Silva Ribeiro ◽  
Arliston Pereira Leite ◽  
Jackson Silva Nóbrega ◽  
Edna Ursulino Alves ◽  
Riselane de Lucena Alcântara Bruno ◽  
...  

Erythroxylum pauferrense is an endemic understory plant species of the Northeast Region of Brazil. The species is of great importance to the region and so ecophysiological studies are needed for its preservation. The objective of the present study was to determine the best substrates and temperatures for testing germination and seed vigor of E. pauferrense. An experiment was performed comprising a completely randomized design in a 5 x 4 factorial scheme, with five temperature regimes (20, 25, 30, 35°C constant and 20-30°C alternating) and four types of substrates (paper, vermiculite, sand and commercial substrate). The following characteristics were evaluated: germination percentage, first germination count, germination speed index, mean germination time, seedling length and dry mass (root and shoot). Paper and vermiculite substrates combined with constant temperatures of 20, 25, 30 and alternated between 20-30°C, provide greater seed germination and vigor while 35°C reduces seed physiological quality.


2014 ◽  
Vol 47 (1) ◽  
pp. 23-29
Author(s):  
S.A. Tabatabaei

ABSTRACT In order to investigate salinity stress on seed reserve utilization and seedling growth of treated seeds of barley (Hordeum vulgare L.), an experiment was carried out. Factorial experiment was carried out in completely randomized design with three replicates. To create salinity stress, NaCl in osmotic levels at 0 (as control), -4, -8, -12 and -16 bar were used. For seed priming, gibberellin (GA) 50 ppm was used. Our results showed that treatment × drought interaction on these traits: germination percentage, weight of utilized (mobilized) seed, seed reserve utilization efficiency, seedling dry weight and seed reserve depletion percentage were significant. The highest germination percentage, weight of utilized (mobilized) seed, seed reserve utilization efficiency, seedling dry weight and seed reserve depletion percentage were attained from priming by gibberellin at control conditions. Thus, priming increased characteristics as compared to the unprimed. Priming improved seed reserve utilization such as: weight of utilized (mobilized) seed reserve, seed reserve depletion percentage, seed reserve utilization efficiency and seedling growth in barley under salinity stress.


2012 ◽  
Vol 45 (1) ◽  
pp. 27-35 ◽  
Author(s):  
F. Khoshsokhan ◽  
M. Babalar ◽  
H. Chaghazardi ◽  
M. Moghadam

Effect of Salinity and Drought Stress on Germination Indices of Two Thymus SpeciesThe environmental stress such as, salinity (soil or water) and drought are serious obstacles for medical plants and field crops in further areas of the world, especially arid and semiarid regions. In order to investigate salinity and drought stress onT. daenensisand T.kotschyanusgermination indices, an experiment was carried out. To create salinity stress, sodium chloride (NaCl) at the levels of 0 (as control), -3, -6 and -9 bar, and for drought stress, polyetylenglycol 6000 (PEG 6000) in osmotic levels at 0 (as control), -3, -6 and -9 bar were used. Effect of drought and salt levels on investigated traits significant at 0.01. Result showed significance different between evaluated indices. Increasing of stress levels lead to reduction of germination and root and shoot length. Salt stress only in high levels reduced germination rate. Therefore,T. daenensisandT. kotschyanushave relative resistance to salinity in germination stage. Decrease in osmotic potential progressively inhibited seed germination of the two thyme species. In all salt treatments, maximum germination percentage inT. kotschyanusrelated to West Azerbaijan population and maximum germination rate related to Gazvin population. But in T. daenensis the highest values (both rate and percentage germination) were observed in Arak population. The germination rate of T.daenensisto the drought stress was more than theT. kotschyanusgermination rate like the germination percentage. Comparing the two stresses, it appears that drought has more effect than salt in germination indices.


2011 ◽  
Vol 3 (4) ◽  
pp. 113-116 ◽  
Author(s):  
Hamdollah ESKANDARI ◽  
Kamyar KAZEMI

Early emergence and stand establishment of cowpea are considered to be the most important yield-contributing factors in rainfed areas. Laboratory tests and afield experiment were conducted in RCB design in 2011 at a research farm in Ramhormoz, Iran, to evaluate the effects of hydropriming (8, 12 and 16 hours duration) and halo priming (solutions of 1.5% KNO3 and 0.8% NaCl) on seedling vigor and field establishment of cowpea. Analysis of variance of laboratory data showed that hydropriming significantly improved germination rate, seed vigor index, and seedling dry weights. However, germination percentage for seeds primed with KNO3 and non-primed seeds were statistically similar, but higher than those for NaCl priming. Overall, hydropriming treatment was comparatively superior in the laboratory tests. Invigoration of cowpea seeds by hydropriming and NaCl priming resulted in higher seedling emergence and establishment in the field, compared to control and seed priming with KNO3. Seedling emergence rate was also enhanced by priming seeds with water, suggesting that hydropriming is a simple, low cost and environmentally friendly technique for improving seed and seedling vigor of cowpea.


Author(s):  
D. J. A. Najorda ◽  
R. J. G. Rosales

The evaluation of seed priming methods on the seed and seedling performance of soursop was conducted January 9 to April 3, 2019 in San Nicolas, Ilocos Norte, Philippines.  The study was conducted to investigate the effect of seed priming methods  capable of breaking dormancy; improve germination rate; determine the seed vigor of soursop; and identify the best seed priming method that provide better seedling performance. The experimental  treatments (unprimed and three priming methods, hydropriming, halopriming and hormonal priming) were laid out in Completely Randomized Design with three replications. A     total of 20 polyethylene bags were used per treatment per replication with one seed sown in every bag. Alternative way to improve seed and seedling performance is the use of these seed priming methods. The seed and seedling performance of soursop were significantly affected by priming methods. Primed seeds had higher percentage germination rate (PGR) than unprimed seeds. But numerically, the highest PGR was hydropriming. Hormonal priming produced significantly taller seedlings at 10 and 40 days after emergence (DAE) than unprimed seeds and more leaves per seedling at 30 to 50 DAE. Hormonal, hydropriming and halopriming produced significantly higher fresh weight of seedlings than unprimed seeds.


2020 ◽  
Vol 1 (6) ◽  
pp. 1-5
Author(s):  
Filza Ghafoor ◽  
Sana Liaqat ◽  
Waseem Iqbal

Seed priming is a pre-sowing treatment used to reduce stress effects in crops .This technique has been used to increase the germination rate of seeds mainly under unfavorable environmental condition. Crop productivity faces many problems like that unavailability of suitable condition that causes unbalanced seedling growth and poor germination. Hormones are essential to improve plant growth, development, crop yield quality and quantity. Auxin is essential hormones that improves germination of seeds and reduce stress effects. Laboratory experiment was conducted to study the effect of seed priming using different concentrations of IAA and IBA on carrot seeds (Daucus carota). In laboratory experiment, three concentrations of IAA and IBA (75 ppm, 50ppm, 25ppm) treated with distilled water with priming duration of 24 hours. Experiment was laid out in CRD with three replications. Data was collected to investigate the effect of seed priming with IAA and IBA under these condition like that (fresh weight, dry weight, speed of germination, root length, seed length, seedling length, final germination rate, mean germination time and dry matter content). Maximum fresh weight, final germination percentage, seedling length, energy of germination, speed of germination and mean germination rate was recorded IBA 75ppm. Maximum dry weight and dry matter content was observed IBA 25ppm.Maximum shoot length was recorded IBA 50ppm. Non-significant difference was recorded in root length.


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