Study of storage on seed germination of Chresta sphaerocephala DC. – Asteraceae

High-altitude fields have been subjected to an intense anthropization process. Due to this, studies on germination, seed storage and propagation of species that compose this plant formation become fundamental. Among such species is the Chresta sphaerocephala DC., Asteraceae. The purpose of this work was to study different temperatures for germination and storage of this species. Four germination temperatures (15°C, 20°C, 25°C and 30°C) and two storage temperatures (-5°C and +5°C) were tested. In order to assess seed vigor, the germination speed index (GSI) and average seed germination time were calculated. ANOVA and Tukey's test were performed at a 5% significance level. In addition, a morphological difference was observed in the analyzed Chresta sphaerocephala seeds, with these being classified as C1 (small seeds), C2 (long thin seeds), C3 (thicker seeds) and C4 (dark colored seeds). As for the storage temperature, 5°C was shown to be the best. When correlated with the germination temperature, 20°C resulted in a statistically higher number of germinated seeds.

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EFFECTS OF STORAGE CONDITIONS AND PRESENCE OF FRUITING BRACTS ON THE GERMINATION OF ATRIPLEX HALIMUS AND SALSOLA VERMICULATA

Experimental Agriculture ◽

10.1017/s0014479797000021 ◽

1997 ◽

Vol 33 (2) ◽

pp. 149-155 ◽

Cited By ~ 16

Author(s):

A. E. OSMAN ◽

F. GHASSALI

Keyword(s):

Seed Germination ◽

Cold Storage ◽

North Africa ◽

Room Temperature ◽

Storage Temperature ◽

Seed Storage ◽

Storage Conditions ◽

Shrub Species ◽

Atriplex Halimus ◽

Different Temperatures

Two shrub species, Atriplex halimus L. and Salsola vermiculata L., are considered useful for rehabilitation of degraded rangelands in west Asia and north Africa. They can be established from direct seeding and are capable of self-sowing. In this study, seed storage at different temperatures and the influence of fruiting bracts on seed germination were examined for the two species during two seasons. Fruits (utricles) were stored at 20–22°C (room temperature), 0°C or −22°C. Germination tests were carried out after 33, 56, 90, 152, 272 and 397 d in storage in the first season and after 44, 76, 104, 170, 288 and 412 d in the second season. Seeds were germinated in their fruiting bracts or after bract removal. Bract removal significantly improved seed germination of both shrubs regardless of storage temperature. For S. vermiculata the increase in germination was in the range of 1.3- to 14.7-fold compared with values for the intact fruit in Season 1 and 0.5 to 3.8 in Season 2. Similarly the ranges for A. halimus were 0.5- to 4.2-fold and 0.7- to 5.3-fold in the two seasons respectively. The effect of cold storage was greater on Salsola than on Atriplex. The reduction of the storage temperature from 21°C to 0°C and −22°C increased the longevity of S. vermiculata seeds by 2.8–46.6 times in Season 1 and by 2.9–2.6 times in Season 2. There was little or no effect on the longevity of A. halimus. A leachate prepared by soaking fruiting bracts from S. vermiculata significantly depressed germination (p < 0.01), the effect being greater on Salsola seeds (20% reduction) than on Atriplex seeds (8% reduction). A leachate from A. halimus produced a slight but non-significant reduction in germination.

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Determination of Optimal Storage Temperature to Maintain Viable Pawpaw [Asimina triloba (L.) Dunal] Seed

HortScience ◽

10.21273/hortsci.35.4.557a ◽

2000 ◽

Vol 35 (4) ◽

pp. 557A-557 ◽

Cited By ~ 1

Author(s):

Kirk W. Pomper ◽

Snake C. Jones ◽

LaTeasa Barnes

Keyword(s):

Native American ◽

Seed Germination ◽

Storage Temperature ◽

Germination Rate ◽

Germplasm Collection ◽

Peat Moss ◽

Short Period ◽

Seed Stratification ◽

And Storage ◽

Storage Temperatures

Pawpaw is a native American tree fruit that has great potential as a new commercial crop. The USDA National Clonal Germplasm Repository for Asimina sp. is located at Kentucky State Univ. (KSU); therefore, germplasm collection and storage are important components of the research program. Recalcitrant seeds do not tolerate desiccation, have a relatively short period of viability, and tend not to tolerate subfreezing temperatures. Since pawpaw seed shows a moderate level of recalcitrance, the objectives of this experiment were to determine which storage temperatures (20, 5, -15, and -70 °C for 8.5 weeks) would maintain viable seed, and whether prior seed stratification (5 °C for 17.5 weeks) would influence survival at the various storage temperatures. Seeds were placed in ziplock bags in moist peat moss and subjected to the range of storage temperatures either before or after stratification. After storage and stratification treatments, seed germination rate was examined for 10 weeks at 25 °C on moist filter paper in petri dishes. Both stratification and storage temperature significantly affected seed germination rate. Seeds did not germinate after storage at subfreezing temperatures, regardless of stratification treatment. The best germination rate, ≈70%, was obtained with stratification followed by storage at 20 °C. However, for long-term storage of viable nongerminating pawpaw seed, stratification followed by storage at 5 °C would be most appropriate. Subfreezing storage temperatures were found to be lethal to pawpaw seeds.

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Effects of Desiccation and Storage Temperature on Seed Germination in Kapok

HortTechnology ◽

10.21273/horttech.26.1.83 ◽

2016 ◽

Vol 26 (1) ◽

pp. 83-88

Author(s):

YanLing Zheng ◽

GaoJuan Zhao ◽

HuanCheng Ma

Keyword(s):

Seed Germination ◽

Storage Temperature ◽

Seed Viability ◽

Germination Percentage ◽

Seed Vigor ◽

Deciduous Tree ◽

Seed Moisture Content ◽

Seed Collection ◽

And Storage ◽

Mature Seeds

Kapok (Bombax ceiba) is a deciduous tree that can grow in the dry-hot valley of southwestern China where its natural regeneration by seedlings is difficult. As mature fruit split open and seeds disperse by wind, it is difficult to collect fully mature seeds. The effects of seed moisture content (MC) and storage temperatures on seed germination of dark-brown seeds collected from split fruit and light-brown seeds collected ≈10–15 days earlier than the time of fruit split were studied to determine the effective germplasm preservation via the seeds. Dark-brown mature seeds could tolerate desiccation to less than 5% MC and could tolerate −20 and −80 °C. Seeds of kapok showed orthodox storage behavior. They can be stored at subzero temperatures with low MC for a long time. For light-brown seeds, germination percentage (GP), germination index (GI), seedling fresh weight (SFW), and vigor index (VI) decreased significantly after seed desiccation. Germination percentage of light-brown seeds with different MC increased to a different extent after being stored at different temperatures for 1 year (76% to 99%), compared with the fresh seeds (73%). Storing fresh seeds at 4 °C was most favorable to keep seed viability and seed vigor of light-brown seeds. Seed collection could be done several days earlier than the time of fruit burst to ensure increased quantity of collected seeds.

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Growth Regulators and Storage Temperature Govern Germination of Coreopsis Seed

HortScience ◽

10.21273/hortsci.27.11.1190 ◽

1992 ◽

Vol 27 (11) ◽

pp. 1190-1193 ◽

Cited By ~ 2

Author(s):

William J. Carpenter ◽

Eric R. Ostmark

Keyword(s):

Relative Humidity ◽

Gibberellic Acid ◽

Growth Regulators ◽

Storage Temperature ◽

Seed Storage ◽

Temperature Regimes ◽

Alternating Temperature ◽

And Storage ◽

Storage Temperatures ◽

Growth Regulator Treatment

The storage and germination environments were evaluated to determine the cause of low total germination percentages and highly irregular germination of Coreopsis lanceolata L. seed. Highest total germination and most rapid and uniform germination of seed occurred at constant 15C, other constant temperatures and all alternating temperature regimes caused lower total germination or delayed it. Seeds tolerated -20C during storage, but total germination was reduced below -5C. Recently harvested seeds had 44% total germination, but 54% to 81% germination was achieved after 6 hours of soaking seeds in 1000 ppm GA3, 1000 ppm ethephon, or 25 ppm kinetin alone or in combination. Growth regulators reduced the number of days to 50% of final germination (T50), and the span in days between 10% and 90% of germination (T90 - T10). Storing fresh seeds without chemical treatment for > 6 months at 5C and 10% to 20% relative humidity (RH), or 15C at 20% to 35% RH, increased total germination to 75% and 80%, respectively. Ten days were required to achieve T50 after 5 to 6 months of storage at 5C and 10% to 20% RH or 15C and 10% to 40% RH, with longer periods to T50 at other storage durations and RH levels. The germination spans (T90 - T10) were lengthened the higher the seed storage temperatures between 5 to 25C, with longer spans as seed storage durations and relative humidities increased. Total germination was similar after storing seeds at 5 or 15C and 10% to 30% RH and after soaking recently harvested seeds in GA3 + ethephon, but the days to T50 and T90 - T10 were shorter after growth regulator treatment. Chemical names used: (2-chloroethyl) phosphonic acid (ethephon); gibberellic acid (GA3); 6-furfurylaminopurine (kinetin).

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Impact of storage conditions on seed germination and seedling growth of wild oat (Avena fatua L.) at different temperatures

Pesticidi i fitomedicina ◽

10.2298/pif1504243s ◽

2015 ◽

Vol 30 (4) ◽

pp. 243-248

Author(s):

Marija Saric-Krsmanovic ◽

Gajic Umiljendic ◽

Ljiljana Santric ◽

Ljiljana Radivojevic

Keyword(s):

Seed Germination ◽

Germination Rate ◽

Seed Storage ◽

Storage Conditions ◽

Avena Fatua ◽

Shoot Length ◽

Wild Oat ◽

Different Temperatures ◽

C 30 ◽

Germinated Seeds

The influence of seed storage conditions and different temperatures (5?C, 10?C, 15?C, 20?C, 25?C, 30?C and 26?C/21?C) during germination and seedling development on seed germination, shoot length and germination rate of wild oat (Avena fatua L.) was examined. Germinated seeds were counted daily over a period of ten days and shoot length was measured on the last day, while germination rates were calculated from those measurements. The results showed that seed storage under controlled conditions (T1: temperature 24?1?C, humidity 40-50%; T2: temperature 26?1?C, humidity 70-80% and T3: temperature 4?C) for periods of 3 (t1) and 12 (t2) months had a significant influence on germination of wild oat seeds. The percentage of germinated seeds under all examined temperatures was higher when they were stored for 12 months under controlled temperature and humidity. The results also showed that temperature had a significant effect on the percentage of germination and germination rate of A. fatua seeds. The highest total germination occurred at 15?C temperature (T1: t1 - 41.25%, t2 - 44.37%; T2: t1 - 28.13%, t2 - 34.37%; T3: t1 - 10.63%, t2 - 12.50%). Germination percentage under an alternating day /night photoperiod at 26?C/21?C temperature was higher in all treatment variants (T1: t1 - 8.13%, t2 - 10.00%; T2: t1 - 11.87%, t2 - 13.13%; T3: t1 - 2.42%, t2 - 2.70%) than germination in the dark at 25?C, 30?C and 5?C.

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Temperature during soybean seed storage and the amount of electrolytes of soaked seeds solution

Scientia Agricola ◽

10.1590/s0103-90162008000500008 ◽

2008 ◽

Vol 65 (5) ◽

pp. 496-501 ◽

Cited By ~ 6

Author(s):

Roberval Daiton Vieira ◽

Dennis Merlin TeKrony ◽

Dennis Bruce Egli ◽

William Patrick Bruenning ◽

Maristela Panobianco

Keyword(s):

Electrical Conductivity ◽

Storage Temperature ◽

Soybean Seed ◽

Storage Period ◽

Seed Storage ◽

Seed Vigor ◽

Soybean Seeds ◽

Glycine Max L ◽

Pea Seeds ◽

And Storage

The electrical conductivity test measures the electrolytes that leach out of seeds when they are immersed in water and this leakage is an indication of seed vigor. The level of standardization reached by the procedures of this test is such that the test is recommended for pea seeds and suggested for other large seeded legumes, including soybean [Glycine max (L.) Merrill]. This study was conducted to contribute to the standardization of this test for soybean seeds by verifying whether the seed storage temperature influences the composition of the leachate from soaked seeds solution. Two soybean seed lots of distinct physiological potential were stored in moisture-proof containers either at constant temperatures of 10ºC and 20ºC or at the temperature of 20ºC during the first seven months of storage followed by a change to 10ºC for the rest of the storage time (nine months). The chemical composition of the soaked water was evaluated every three months from January to October 1998. The highest amount of leakage was observed for potassium, followed by calcium and magnesium, iron and sodium regardless of temperature and storage period. The amount of electrolytes in the soaked water increased as the period of time and the temperature of storage increased. On the other hand the amount of leakage decrease along the time for those seeds stored at 10ºC or transferred from the temperature of 20 to that of 10ºC. The temperature at which soybean seeds remain during storage may affect the amount of electrolytes in the soaked water and consequently the results of the electrical conductivity test.

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Changes of Physicochemical Properties of Starch Syrups Recommended for Winter Feeding of Honeybees during Storage

Agriculture ◽

10.3390/agriculture11040374 ◽

2021 ◽

Vol 11 (4) ◽

pp. 374

Author(s):

Teresa Szczęsna ◽

Ewa Waś ◽

Piotr Semkiw ◽

Piotr Skubida ◽

Katarzyna Jaśkiewicz ◽

...

Keyword(s):

Chemical Composition ◽

Physicochemical Properties ◽

Physicochemical Parameters ◽

Storage Temperature ◽

Storage Conditions ◽

Different Temperatures ◽

And Storage ◽

Bee Colonies ◽

Temperature Time ◽

Winter Feeding

The aim of this study was to determine the influence of storage temperature and time on physicochemical parameters of starch syrups recommended for the winter feeding of bee colonies. The studies included commercially available three starch syrups and an inverted saccharose syrup that were stored at different temperatures: ca. 20 °C, 10–14 °C, and ca. 4 °C. Physicochemical parameters of fresh syrups (immediately after purchase) and syrups after 3, 6, 9, 12, 15, 18, 21, and 24 months of storage at the abovementioned temperatures were measured. It was observed that the rate of unfavorable changes in chemical composition of starch syrups and the inverted saccharose syrup, mainly the changes in the 5-hydroxymethylfurfural (HMF) content, depended on the type of a syrup and storage conditions (temperature, time). Properties of tested starch syrups intended for winter feeding of bees stored at ca. 20 °C maintained unchanged for up to 6 months, whereas the same syrups stored at lower temperatures (10–14 °C) maintained unchanged physicochemical parameters for about 12 months. In higher temperatures, the HMF content increased. To date, the influence of this compound on bees has not been thoroughly investigated.

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Influence of Heat on Seed Germination of Cistus Laurifolius and Cistus Ladanifer

International Journal of Wildland Fire ◽

10.1071/wf9920015 ◽

1992 ◽

Vol 2 (1) ◽

pp. 15 ◽

Cited By ~ 47

Author(s):

L Valbuena ◽

R Tarrega ◽

E Luis

Keyword(s):

Seed Germination ◽

High Temperatures ◽

Exposure Time ◽

Germination Rate ◽

Heat Exposure ◽

Cistus Ladanifer ◽

Different Temperatures ◽

Petri Dishes ◽

Germinated Seeds

The influence of high temperatures on germination of Cistus laurifolius and Cistus ladanifer seeds was analyzed. Seeds were subjected to different temperatures for different times, afterwards they were sowed in plastic petri dishes and monitored for germinated seeds over two months.The germination rate observed in Cistus ldanifer was greater than in Cistus laurifolius. In both species, heat increased germination percentages. For Cistus laurifolius higher temperatures or longer exposure times were needed. Germination percentages of Cistus ladanifer were lower when heat exposure time was 15 minutes.It must be emphasized that germination occurred when seeds were not treated, while seeds exposed to 150�C for 5 minutes or more did not germinate.

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Shelf Life Studies of Three Wasabi Flavoured Sauces

Bangladesh Journal of Scientific and Industrial Research ◽

10.3329/bjsir.v44i2.3665 ◽

1970 ◽

Vol 44 (2) ◽

pp. 147-156

Author(s):

Tamanna Sultana ◽

GP Savage ◽

NG Porter ◽

DL McNeil ◽

JR Sedcole

Keyword(s):

Shelf Life ◽

Storage Temperature ◽

Initial Level ◽

Tomato Sauce ◽

Wasabia Japonica ◽

Different Temperatures ◽

Small Change ◽

Shelf Life Studies ◽

The Stability ◽

Storage Temperatures

Isothiocyanates (ITCs) contained in purees extracted from wasabi (Wasabia japonica (Miq) Matsum) can be used to manufacture a range of interesting spicy foods. In New Zealand, local manufacturers are showing interest in producing various forms of processed wasabi based sauces. However, isothiocyanates have been shown to degrade quickly in some situations. Therefore, in this study, the stability of allyl ITC was investigated in three wasabi flavoured products stored at four different temperatures (4, 10, 20 and 30°C) for 22 weeks. Two creamy (mayonnaise and tartare) sauces and a non-creamy sauce were prepared from an original recipe and flavoured with a known volume of "wasabi oil". Two types of pouches (clear and metallic plastic) were used to store each product and allyl ITC content was measured in the stored sauces at two week intervals. The initial level of allyl ITC found in mayonnaise, tartare and smoky tomato sauces were 415.3, 411.4 and 144.7 mg/ kg respectively, prior to storage. Temperature showed a strong influence in reducing allyl ITC (P=0.005 to <0.001) but no significant effect was identified for the two types of packets used. The non-creamy smoky tomato sauce was very unstable at 10°C or higher temperatures and the allyl ITC contents reduced rapidly with increasing storage temperatures. For instance, at 30°C, a 66% loss occurred by week 2 and a 90% loss occurred by week 6 in the smoky tomato sauce. However, mayonnaise and tartare sauces had a shelf life of 8 to 9 weeks with only a marginal reduction in allyl ITC (2% overall) at all the stored temperatures (4-30°C). These creamy sauces were characterized by a sudden fall in 10 weeks ending in a 69-70% loss of allyl ITC at 22 weeks. No microbial growth occurred in any of the sauces stored at any of the temperatures during the course of this storage experiment though very small change of colour was noticed for the sauces when stored at 30°C. Keywords: Bangladesh J. Sci. Ind. Res. 44(2), 147-156, 2009DOI: 10.3329/bjsir.v44i2.3665Bangladesh J. Sci. Ind. Res. 44(2), 147-156, 2009

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Modeling the Effect of Water Activity, pH, and Temperature on the Probability of Enterotoxin A Production by Staphylococcus aureus

Journal of Food Protection ◽

10.4315/0362-028x.jfp-15-161 ◽

2016 ◽

Vol 79 (1) ◽

pp. 148-152 ◽

Cited By ~ 5

Author(s):

TIAN DING ◽

YAN-YAN YU ◽

CHENG-AN HWANG ◽

QING-LI DONG ◽

SHI-GUO CHEN ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Water Activity ◽

Storage Temperature ◽

Probability Model ◽

Toxin Production ◽

Factors Affecting ◽

Model Predictions ◽

And Storage ◽

Storage Temperatures ◽

Good Agreement

ABSTRACT The objectives of this study were to develop a probability model of Staphylococcus aureus enterotoxin A (SEA) production as affected by water activity (aw), pH, and temperature in broth and assess its applicability for milk. The probability of SEA production was assessed in tryptic soy broth using 24 combinations of aw (0.86 to 0.99), pH (5.0 to 7.0), and storage temperature (10 to 30°C). The observed probabilities were fitted with a logistic regression to develop a probability model. The model had a concordant value of 97.5% and concordant index of 0.98, indicating that the model satisfactorily describes the probability of SEA production. The model showed that aw, pH, and temperature were significant factors affecting the probability of toxin production. The model predictions were in good agreement with the observed values obtained from milk. The model may help manufacturers in selecting product pH and aw and storage temperatures to prevent SEA production.

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